ES2179760A1 - Genes codificantes de beta-agarasas y su utilizacion para producir enzimas de biodegradacion de agares. - Google Patents
Genes codificantes de beta-agarasas y su utilizacion para producir enzimas de biodegradacion de agares.Info
- Publication number
- ES2179760A1 ES2179760A1 ES200050080A ES200050080A ES2179760A1 ES 2179760 A1 ES2179760 A1 ES 2179760A1 ES 200050080 A ES200050080 A ES 200050080A ES 200050080 A ES200050080 A ES 200050080A ES 2179760 A1 ES2179760 A1 ES 2179760A1
- Authority
- ES
- Spain
- Prior art keywords
- beta
- seq
- agaa
- dsm
- drobachiensis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01081—Beta-agarase (3.2.1.81)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
GENES CODIFICANTES DE B-AGARASAS Y SU UTILIZACION PARA PRODUCIR ENZIMAS DE BIODEGRADACION DE AGARES. La invención se relaciona con la nueva cepa de Cytophaga drobachiensis depositada en la Colección DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen Gmbh (Colección Alemana de microorganismos y Cultivos Celulares)) el 8 de mayo de 1.998 bajo el número DSM 12170, con el gen agaA codificante de una B-agarasa y que tiene la SEC ID N 1, con el gen agaB codificante de una B-agarasa y que tiene la SEC ID N.3, cuyos genes codifican para una B-agarasa de Cytophaga drobachiensis DSM 12170, y con la secuencia específica de ácido nucleico del gen agaA codificante de un fragmento peptídico específico AgaA'' que tiene conservada la actividad B-agarasa y que tiene la SEC ID N 5, así como con la proteína AgaA de C. drobachiensis DSM 12170 que tiene la SEC ID nN 2, la proteína AgaA de C. drobachiiensis DSM 12170 que tiene la SEC ID N 4 y dicho fragmento peptídico AgaA'' de C. drobachiensis DSM 12170que tiene la SEC ID N 6.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES200200647A ES2214936B1 (es) | 1998-06-12 | 2002-03-18 | Gen codificante de una beta-agarasa y su utilizacion para producir enzimas de biodegradacion de agares. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR9807419A FR2779736B1 (fr) | 1998-06-12 | 1998-06-12 | Genes codant pour des beta-agarases et leur utilisation pour la production d'enzymes de biodegradation des agars |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2179760A1 true ES2179760A1 (es) | 2003-01-16 |
ES2179760B1 ES2179760B1 (es) | 2004-03-16 |
Family
ID=9527318
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES200050080A Expired - Fee Related ES2179760B1 (es) | 1998-06-12 | 1999-06-11 | Genes codificantes de beta-agarasas y su utilizacion para producir enzimas de biodegradacion de agares. |
ES200200647A Expired - Fee Related ES2214936B1 (es) | 1998-06-12 | 2002-03-18 | Gen codificante de una beta-agarasa y su utilizacion para producir enzimas de biodegradacion de agares. |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES200200647A Expired - Fee Related ES2214936B1 (es) | 1998-06-12 | 2002-03-18 | Gen codificante de una beta-agarasa y su utilizacion para producir enzimas de biodegradacion de agares. |
Country Status (7)
Country | Link |
---|---|
US (1) | US6511838B1 (es) |
AU (1) | AU4148699A (es) |
DE (1) | DE19983297B3 (es) |
ES (2) | ES2179760B1 (es) |
FR (1) | FR2779736B1 (es) |
GB (1) | GB2354000B (es) |
WO (1) | WO1999066052A1 (es) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100601331B1 (ko) * | 2001-02-27 | 2006-07-14 | 다카라 바이오 가부시키가이샤 | 아가레이즈 및 그의 유전자 |
BRPI0916186B8 (pt) | 2008-07-17 | 2021-05-25 | Medigen Inc | vetor de plasmídeo e seu uso, vacina para febre amarela e para vírus de rna infeccioso e vírus atenuado vivo clonalmente purificado homogêneo e seu uso |
CN110066779A (zh) * | 2019-04-17 | 2019-07-30 | 武汉轻工大学 | 琼脂糖酶基因、重组质粒、重组菌株、琼脂糖酶及其应用 |
CN114934033B (zh) * | 2022-03-04 | 2023-08-04 | 青岛海洋生物医药研究院股份有限公司 | 一种琼胶酶突变体及其编码基因和应用 |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5418156A (en) * | 1991-04-15 | 1995-05-23 | University Of Maryland | Agarase enzyme system from alteromonas strain 2-40 |
-
1998
- 1998-06-12 FR FR9807419A patent/FR2779736B1/fr not_active Expired - Lifetime
-
1999
- 1999-06-11 ES ES200050080A patent/ES2179760B1/es not_active Expired - Fee Related
- 1999-06-11 GB GB0030283A patent/GB2354000B/en not_active Expired - Lifetime
- 1999-06-11 US US09/719,402 patent/US6511838B1/en not_active Expired - Lifetime
- 1999-06-11 AU AU41486/99A patent/AU4148699A/en not_active Abandoned
- 1999-06-11 WO PCT/FR1999/001397 patent/WO1999066052A1/fr active IP Right Grant
- 1999-06-11 DE DE19983297T patent/DE19983297B3/de not_active Expired - Lifetime
-
2002
- 2002-03-18 ES ES200200647A patent/ES2214936B1/es not_active Expired - Fee Related
Non-Patent Citations (4)
Title |
---|
BARBEYRON, T. et al. "The Kappa-carrageenase of the marine bacterium Cytophaga drobachiensis. Structural and phylogenetic relationships within family-16 glycoside hydrolases". MOLECULAR BIOLOGY AND EVOLUTION, 1998, Vol. 15, Nö 5, páginas 528-537. Todo el documento. * |
DUCKWORTH, M. et al. "The action of a bacterial agarase on agarose, porphyran and alkali-treated porphyran". BIOCHEMICAL JOURNAL, 1969, Vol. 113, pßginas 687-692. Todo el documento. * |
POTIN, P. et al. "Purification and characterization of a new K-carrageenase from a marine Cytophaga-like bacterium". EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, Vol. 201, Nö 1, páginas 241-247. Todo el documento. * |
VAN DER MEULEN, H.J. et al. "Production and characterization of the agarase of Cytophaga flevensis". ANTONIE VAN LEEUWENHOEK, 1975, Vol. 41, pßginas 431-447. Todo el documento. * |
Also Published As
Publication number | Publication date |
---|---|
ES2214936A1 (es) | 2004-09-16 |
DE19983297T1 (de) | 2001-05-10 |
ES2179760B1 (es) | 2004-03-16 |
ES2214936B1 (es) | 2005-12-16 |
FR2779736A1 (fr) | 1999-12-17 |
US6511838B1 (en) | 2003-01-28 |
GB2354000B (en) | 2004-01-07 |
GB2354000A (en) | 2001-03-14 |
DE19983297B3 (de) | 2013-07-04 |
AU4148699A (en) | 2000-01-05 |
GB0030283D0 (en) | 2001-01-24 |
WO1999066052A1 (fr) | 1999-12-23 |
FR2779736B1 (fr) | 2002-12-13 |
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Legal Events
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