ES2173894T3 - Incremento de la coloracion inmunoloquimica en tejidos fijados por aldehido. - Google Patents

Incremento de la coloracion inmunoloquimica en tejidos fijados por aldehido.

Info

Publication number
ES2173894T3
ES2173894T3 ES93919972T ES93919972T ES2173894T3 ES 2173894 T3 ES2173894 T3 ES 2173894T3 ES 93919972 T ES93919972 T ES 93919972T ES 93919972 T ES93919972 T ES 93919972T ES 2173894 T3 ES2173894 T3 ES 2173894T3
Authority
ES
Spain
Prior art keywords
aldehido
fabric
immunolochemical
coloration
increment
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93919972T
Other languages
English (en)
Inventor
Shan-Rong Shi
Atul K Tandon
Krishan L Kalra
Nagesh Malhotra
Sheng-Hui Su
Cheng-Zhi Yu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biogenex Laboratories Inc
Original Assignee
Biogenex Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biogenex Laboratories Inc filed Critical Biogenex Laboratories Inc
Application granted granted Critical
Publication of ES2173894T3 publication Critical patent/ES2173894T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding

Abstract

UN METODO PARA REPONER LA INMUNOREACTIVIDAD DE UN TEJIDO,PARTICULARMENTE UN TEJIDO DESCALCIFICADO COMPUESTO DE UN AGENTE FIJADOR ALDEHIDO Y ASENTADO EN UN MEDIO ALOJADOR QUE COMPRENDE NORMALMENTE CELOIDINA, COMPRENDE LOS PASOS DE: CONTACTAR EL TEJIDO CON UNA DISOLUCION REACTIVA LIBERADORA DE ALDEHIDO COMPUESTA DE UN DISOLVENTE Y UN REACTIVO LIBERADOR DE ALDEHIDO Y; ELIMINAR EL ALDEHIDO LIBERADO MEDIANTE EL REACTIVO LIBERADOR A PARTIR DEL CONTACTO CON EL TEJIDO, REACCIONANDO EL ALDEHIDO DE FORMA SUSTANCIALMENTE IRREVERSIBLE PARA FORMAR UN DERIVADO NO ALDEHIDO Y; ELIMINAR EL EXCESO DE BASE A PARTIR DEL TEJIDO. UNA DISOLUCION REFERIDA PARA UN TEJIDO DESCALCIFICADO ASENTADO EN CELOIDINA COMPRENDE UN HIDROXIDO SODICO METANOLICO DE SATURACION ALREDEDOR DE UNO-TRES.
ES93919972T 1992-08-12 1993-08-12 Incremento de la coloracion inmunoloquimica en tejidos fijados por aldehido. Expired - Lifetime ES2173894T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US92896292A 1992-08-12 1992-08-12

Publications (1)

Publication Number Publication Date
ES2173894T3 true ES2173894T3 (es) 2002-11-01

Family

ID=25457093

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93919972T Expired - Lifetime ES2173894T3 (es) 1992-08-12 1993-08-12 Incremento de la coloracion inmunoloquimica en tejidos fijados por aldehido.

Country Status (5)

Country Link
EP (1) EP0607422B1 (es)
JP (1) JP3108099B2 (es)
DE (1) DE69331684T2 (es)
ES (1) ES2173894T3 (es)
WO (1) WO1994004906A1 (es)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1005633T3 (da) * 1997-08-20 2008-12-15 Univ Miami Vævsfikserings-dehydratiserings-fedtfjernelses-imprægneringsmetode med höj kvalitet og kontinuerligt gennemlöb
US6649368B1 (en) * 1997-10-24 2003-11-18 Cell Marque Corporation Composition and method for treating tissue samples
WO2000014507A1 (en) * 1998-09-03 2000-03-16 Ventana Medical Systems, Inc. Removal of embedding media from biological samples and cell conditioning on automated staining instruments
US6855559B1 (en) 1998-09-03 2005-02-15 Ventana Medical Systems, Inc. Removal of embedding media from biological samples and cell conditioning on automated staining instruments
US7410753B2 (en) 1998-09-03 2008-08-12 Ventana Medical Systems, Inc. Removal of embedding media from biological samples and cell conditioning on automated staining instruments
US6855552B2 (en) 1998-09-03 2005-02-15 Ventana Medical Systems Automated immunohistochemical and in situ hybridization assay formulations
US6544798B1 (en) * 1999-02-26 2003-04-08 Ventana Medical Systems, Inc. Removal of embedding media from biological samples and cell conditioning on automated staining instruments
DE60229365D1 (de) * 2002-06-26 2008-11-27 Shigeki Namimatsu Verfahren zur Aktivierung von Antigenen
JP4251627B2 (ja) * 2003-09-19 2009-04-08 株式会社東芝 化学分析装置及びその分注方法
US8012693B2 (en) * 2003-12-16 2011-09-06 3M Innovative Properties Company Analysis of chemically crosslinked cellular samples
CN105441549A (zh) 2007-03-30 2016-03-30 霍夫曼-拉罗奇有限公司 烷基胺改进甲醛固定的生物样品组分的检测
EP2521778B1 (en) * 2010-01-04 2014-03-19 QIAGEN Gaithersburg, Inc. Methods, compositions, and kits for recovery of nucleic acids or proteins from fixed tissue samples
DE102012101896A1 (de) * 2012-03-06 2013-09-12 Roberto Gerigk Formalinfreie Fixierungsmittel für histologische Färbungen von Gewebeproben
US10018540B2 (en) 2012-04-10 2018-07-10 Rutgers, The State University Of New Jersey Clearing agent and mounting media for microscopy
US9464971B2 (en) 2012-04-10 2016-10-11 Rutgers, The State University Of New Jersey Clearing agent and mounting medium for microscopy
US9506928B2 (en) * 2013-12-12 2016-11-29 University Of Houston System Methods for retrieving antigens using aldehyde scavenging agents
CA3197977A1 (en) * 2020-11-11 2022-05-19 Lance A. Liotta Laser capture microdissection visualization chemistry

Also Published As

Publication number Publication date
EP0607422A1 (en) 1994-07-27
EP0607422B1 (en) 2002-03-13
DE69331684T2 (de) 2002-10-31
EP0607422A4 (en) 1996-10-16
DE69331684D1 (de) 2002-04-18
JPH07500674A (ja) 1995-01-19
JP3108099B2 (ja) 2000-11-13
WO1994004906A1 (en) 1994-03-03

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