DE69331684T2 - Verbesserung der immunochemischen anfärbung in aldehyd-fixierten geweben - Google Patents

Verbesserung der immunochemischen anfärbung in aldehyd-fixierten geweben

Info

Publication number
DE69331684T2
DE69331684T2 DE69331684T DE69331684T DE69331684T2 DE 69331684 T2 DE69331684 T2 DE 69331684T2 DE 69331684 T DE69331684 T DE 69331684T DE 69331684 T DE69331684 T DE 69331684T DE 69331684 T2 DE69331684 T2 DE 69331684T2
Authority
DE
Germany
Prior art keywords
aldehyd
improvement
fixed tissues
immunochemical staining
immunochemical
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69331684T
Other languages
English (en)
Other versions
DE69331684D1 (de
Inventor
Shan-Rong Shi
K Tandon
L Kalra
Nagesh Malhotra
Sheng-Hui Su
Cheng-Zhi Yu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BIOGENEX LAB SAN RAMON
Biogenex Laboratories Inc
Original Assignee
BIOGENEX LAB SAN RAMON
Biogenex Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BIOGENEX LAB SAN RAMON, Biogenex Laboratories Inc filed Critical BIOGENEX LAB SAN RAMON
Publication of DE69331684D1 publication Critical patent/DE69331684D1/de
Application granted granted Critical
Publication of DE69331684T2 publication Critical patent/DE69331684T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5306Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
DE69331684T 1992-08-12 1993-08-12 Verbesserung der immunochemischen anfärbung in aldehyd-fixierten geweben Expired - Fee Related DE69331684T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US92896292A 1992-08-12 1992-08-12
PCT/US1993/007550 WO1994004906A1 (en) 1992-08-12 1993-08-12 Enhancement of immunochemical staining in aldehyde-fixed tissues

Publications (2)

Publication Number Publication Date
DE69331684D1 DE69331684D1 (de) 2002-04-18
DE69331684T2 true DE69331684T2 (de) 2002-10-31

Family

ID=25457093

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69331684T Expired - Fee Related DE69331684T2 (de) 1992-08-12 1993-08-12 Verbesserung der immunochemischen anfärbung in aldehyd-fixierten geweben

Country Status (5)

Country Link
EP (1) EP0607422B1 (de)
JP (1) JP3108099B2 (de)
DE (1) DE69331684T2 (de)
ES (1) ES2173894T3 (de)
WO (1) WO1994004906A1 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102012101896A1 (de) * 2012-03-06 2013-09-12 Roberto Gerigk Formalinfreie Fixierungsmittel für histologische Färbungen von Gewebeproben

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6207408B1 (en) * 1997-08-20 2001-03-27 University Of Miami High quality, continuous throughput, tissue fixation-dehydration-fat removal-impregnation method
US6649368B1 (en) * 1997-10-24 2003-11-18 Cell Marque Corporation Composition and method for treating tissue samples
US6855559B1 (en) 1998-09-03 2005-02-15 Ventana Medical Systems, Inc. Removal of embedding media from biological samples and cell conditioning on automated staining instruments
EP1112479B1 (de) * 1998-09-03 2018-08-15 Ventana Medical Systems, Inc. Automatische entfernung von einbettungsmedien von biologischen proben
US7410753B2 (en) 1998-09-03 2008-08-12 Ventana Medical Systems, Inc. Removal of embedding media from biological samples and cell conditioning on automated staining instruments
US6855552B2 (en) 1998-09-03 2005-02-15 Ventana Medical Systems Automated immunohistochemical and in situ hybridization assay formulations
US6544798B1 (en) * 1999-02-26 2003-04-08 Ventana Medical Systems, Inc. Removal of embedding media from biological samples and cell conditioning on automated staining instruments
DE60229365D1 (de) * 2002-06-26 2008-11-27 Shigeki Namimatsu Verfahren zur Aktivierung von Antigenen
JP4251627B2 (ja) * 2003-09-19 2009-04-08 株式会社東芝 化学分析装置及びその分注方法
US8012693B2 (en) * 2003-12-16 2011-09-06 3M Innovative Properties Company Analysis of chemically crosslinked cellular samples
EP2132549B1 (de) * 2007-03-30 2013-09-11 Roche Diagnostics GmbH Alkylamine zur verbesserten erkennung von komponenten formaldehyd-fixierter biologischer proben
EP2521778B1 (de) * 2010-01-04 2014-03-19 QIAGEN Gaithersburg, Inc. Verfahren, zusammensetzungen und kits zur gewinnung von nukleinsäuren oder proteinen aus festen gewebeproben
US10018540B2 (en) 2012-04-10 2018-07-10 Rutgers, The State University Of New Jersey Clearing agent and mounting media for microscopy
WO2013155064A1 (en) * 2012-04-10 2013-10-17 Rutgers, The State University Of New Jersey Clearing agent and mounting medium for microscopy
WO2015088667A1 (en) * 2013-12-12 2015-06-18 University Of Houston System Universal antigen retrieval compounds and methods of use
US20230384187A1 (en) * 2020-11-11 2023-11-30 George Mason Research Foundation, Inc. Laser capture microdissection visualization chemistry

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102012101896A1 (de) * 2012-03-06 2013-09-12 Roberto Gerigk Formalinfreie Fixierungsmittel für histologische Färbungen von Gewebeproben

Also Published As

Publication number Publication date
EP0607422A4 (en) 1996-10-16
EP0607422A1 (de) 1994-07-27
JP3108099B2 (ja) 2000-11-13
JPH07500674A (ja) 1995-01-19
DE69331684D1 (de) 2002-04-18
WO1994004906A1 (en) 1994-03-03
EP0607422B1 (de) 2002-03-13
ES2173894T3 (es) 2002-11-01

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee