DE69331684T2 - Verbesserung der immunochemischen anfärbung in aldehyd-fixierten geweben - Google Patents
Verbesserung der immunochemischen anfärbung in aldehyd-fixierten gewebenInfo
- Publication number
- DE69331684T2 DE69331684T2 DE69331684T DE69331684T DE69331684T2 DE 69331684 T2 DE69331684 T2 DE 69331684T2 DE 69331684 T DE69331684 T DE 69331684T DE 69331684 T DE69331684 T DE 69331684T DE 69331684 T2 DE69331684 T2 DE 69331684T2
- Authority
- DE
- Germany
- Prior art keywords
- aldehyd
- improvement
- fixed tissues
- immunochemical staining
- immunochemical
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 230000000984 immunochemical effect Effects 0.000 title 1
- 238000010186 staining Methods 0.000 title 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US92896292A | 1992-08-12 | 1992-08-12 | |
PCT/US1993/007550 WO1994004906A1 (en) | 1992-08-12 | 1993-08-12 | Enhancement of immunochemical staining in aldehyde-fixed tissues |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69331684D1 DE69331684D1 (de) | 2002-04-18 |
DE69331684T2 true DE69331684T2 (de) | 2002-10-31 |
Family
ID=25457093
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69331684T Expired - Fee Related DE69331684T2 (de) | 1992-08-12 | 1993-08-12 | Verbesserung der immunochemischen anfärbung in aldehyd-fixierten geweben |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP0607422B1 (de) |
JP (1) | JP3108099B2 (de) |
DE (1) | DE69331684T2 (de) |
ES (1) | ES2173894T3 (de) |
WO (1) | WO1994004906A1 (de) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE102012101896A1 (de) * | 2012-03-06 | 2013-09-12 | Roberto Gerigk | Formalinfreie Fixierungsmittel für histologische Färbungen von Gewebeproben |
Families Citing this family (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6207408B1 (en) * | 1997-08-20 | 2001-03-27 | University Of Miami | High quality, continuous throughput, tissue fixation-dehydration-fat removal-impregnation method |
US6649368B1 (en) * | 1997-10-24 | 2003-11-18 | Cell Marque Corporation | Composition and method for treating tissue samples |
US6855559B1 (en) | 1998-09-03 | 2005-02-15 | Ventana Medical Systems, Inc. | Removal of embedding media from biological samples and cell conditioning on automated staining instruments |
EP1112479B1 (de) * | 1998-09-03 | 2018-08-15 | Ventana Medical Systems, Inc. | Automatische entfernung von einbettungsmedien von biologischen proben |
US7410753B2 (en) | 1998-09-03 | 2008-08-12 | Ventana Medical Systems, Inc. | Removal of embedding media from biological samples and cell conditioning on automated staining instruments |
US6855552B2 (en) | 1998-09-03 | 2005-02-15 | Ventana Medical Systems | Automated immunohistochemical and in situ hybridization assay formulations |
US6544798B1 (en) * | 1999-02-26 | 2003-04-08 | Ventana Medical Systems, Inc. | Removal of embedding media from biological samples and cell conditioning on automated staining instruments |
DE60229365D1 (de) * | 2002-06-26 | 2008-11-27 | Shigeki Namimatsu | Verfahren zur Aktivierung von Antigenen |
JP4251627B2 (ja) * | 2003-09-19 | 2009-04-08 | 株式会社東芝 | 化学分析装置及びその分注方法 |
US8012693B2 (en) * | 2003-12-16 | 2011-09-06 | 3M Innovative Properties Company | Analysis of chemically crosslinked cellular samples |
EP2132549B1 (de) * | 2007-03-30 | 2013-09-11 | Roche Diagnostics GmbH | Alkylamine zur verbesserten erkennung von komponenten formaldehyd-fixierter biologischer proben |
EP2521778B1 (de) * | 2010-01-04 | 2014-03-19 | QIAGEN Gaithersburg, Inc. | Verfahren, zusammensetzungen und kits zur gewinnung von nukleinsäuren oder proteinen aus festen gewebeproben |
US10018540B2 (en) | 2012-04-10 | 2018-07-10 | Rutgers, The State University Of New Jersey | Clearing agent and mounting media for microscopy |
WO2013155064A1 (en) * | 2012-04-10 | 2013-10-17 | Rutgers, The State University Of New Jersey | Clearing agent and mounting medium for microscopy |
WO2015088667A1 (en) * | 2013-12-12 | 2015-06-18 | University Of Houston System | Universal antigen retrieval compounds and methods of use |
US20230384187A1 (en) * | 2020-11-11 | 2023-11-30 | George Mason Research Foundation, Inc. | Laser capture microdissection visualization chemistry |
-
1993
- 1993-08-12 WO PCT/US1993/007550 patent/WO1994004906A1/en active IP Right Grant
- 1993-08-12 DE DE69331684T patent/DE69331684T2/de not_active Expired - Fee Related
- 1993-08-12 JP JP06506381A patent/JP3108099B2/ja not_active Expired - Fee Related
- 1993-08-12 EP EP93919972A patent/EP0607422B1/de not_active Expired - Lifetime
- 1993-08-12 ES ES93919972T patent/ES2173894T3/es not_active Expired - Lifetime
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE102012101896A1 (de) * | 2012-03-06 | 2013-09-12 | Roberto Gerigk | Formalinfreie Fixierungsmittel für histologische Färbungen von Gewebeproben |
Also Published As
Publication number | Publication date |
---|---|
EP0607422A4 (en) | 1996-10-16 |
EP0607422A1 (de) | 1994-07-27 |
JP3108099B2 (ja) | 2000-11-13 |
JPH07500674A (ja) | 1995-01-19 |
DE69331684D1 (de) | 2002-04-18 |
WO1994004906A1 (en) | 1994-03-03 |
EP0607422B1 (de) | 2002-03-13 |
ES2173894T3 (es) | 2002-11-01 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition | ||
8339 | Ceased/non-payment of the annual fee |