ES2167278B1 - Enzima con actividad peroxidasa aislada de la alcachofa (cynara scolymus, l.) procedimiento para su aislamiento y purificacion y aplicaciones. - Google Patents

Enzima con actividad peroxidasa aislada de la alcachofa (cynara scolymus, l.) procedimiento para su aislamiento y purificacion y aplicaciones.

Info

Publication number
ES2167278B1
ES2167278B1 ES200002553A ES200002553A ES2167278B1 ES 2167278 B1 ES2167278 B1 ES 2167278B1 ES 200002553 A ES200002553 A ES 200002553A ES 200002553 A ES200002553 A ES 200002553A ES 2167278 B1 ES2167278 B1 ES 2167278B1
Authority
ES
Spain
Prior art keywords
enzyme
artichoke
peroxidase activity
purification
applications
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES200002553A
Other languages
English (en)
Other versions
ES2167278A1 (es
Inventor
Lopez Jose Neptuno Rodriguez
Molina Dorotea Lopez
Serrano Jose Bautista Tudela
Canovas Francisco Garcia
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universidad de Murcia
Original Assignee
Universidad de Murcia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universidad de Murcia filed Critical Universidad de Murcia
Priority to ES200002553A priority Critical patent/ES2167278B1/es
Priority to AU2001295630A priority patent/AU2001295630A1/en
Priority to PCT/ES2001/000400 priority patent/WO2002034898A1/es
Priority to EP01976325A priority patent/EP1336653A1/en
Publication of ES2167278A1 publication Critical patent/ES2167278A1/es
Application granted granted Critical
Publication of ES2167278B1 publication Critical patent/ES2167278B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Enzima con actividad peroxidasa aislada de la alcachofa (Cynara scolymus, L.), procedimiento para su aislamiento y purificación y aplicaciones. La enzima con actividad peroxidasa aislada a partir de alcachofa (Cynara scolymus, L.) es una proteína glicosilada que puede obtenerse mediante un procedimiento que comprende la extracción de dicha enzima con actividad peroxidasa a partir de tejidos de alcachofa, la separación de dicha enzima de otras enzimas con actividad oxidasa eventualmente presentes en el material vegetal, y, opcionalmente, la purificación parcial o a homogeneidad de dicha enzima con actividad peroxidasa. Esta enzima puede utilizarse en la oxidación de sustratos reductores, como reactivo clínico o de diagnóstico, como enzima marcadora en inmunoensayos, para la elaboración de biosensores, como biocatalizador para la obtención de productos de interés biotecnológico e industrial y en aplicaciones medioambientales.
ES200002553A 2000-10-24 2000-10-24 Enzima con actividad peroxidasa aislada de la alcachofa (cynara scolymus, l.) procedimiento para su aislamiento y purificacion y aplicaciones. Expired - Fee Related ES2167278B1 (es)

Priority Applications (4)

Application Number Priority Date Filing Date Title
ES200002553A ES2167278B1 (es) 2000-10-24 2000-10-24 Enzima con actividad peroxidasa aislada de la alcachofa (cynara scolymus, l.) procedimiento para su aislamiento y purificacion y aplicaciones.
AU2001295630A AU2001295630A1 (en) 2000-10-24 2001-10-24 Enzyme with peroxidase activity isolated from artichoke (cynara scolymus, l.), process for isolation and purification and applications
PCT/ES2001/000400 WO2002034898A1 (es) 2000-10-24 2001-10-24 Enzima con actividad peroxidasa aislada de la alcachofa cynara scolymus, l., procedimiento para su aislamiento y purificación y aplicaciones
EP01976325A EP1336653A1 (en) 2000-10-24 2001-10-24 Enzyme with peroxidase activity isolated from artichoke (cynara scolymus, l.), process for isolation and purification and applications

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES200002553A ES2167278B1 (es) 2000-10-24 2000-10-24 Enzima con actividad peroxidasa aislada de la alcachofa (cynara scolymus, l.) procedimiento para su aislamiento y purificacion y aplicaciones.

Publications (2)

Publication Number Publication Date
ES2167278A1 ES2167278A1 (es) 2002-05-01
ES2167278B1 true ES2167278B1 (es) 2003-08-16

Family

ID=8495379

Family Applications (1)

Application Number Title Priority Date Filing Date
ES200002553A Expired - Fee Related ES2167278B1 (es) 2000-10-24 2000-10-24 Enzima con actividad peroxidasa aislada de la alcachofa (cynara scolymus, l.) procedimiento para su aislamiento y purificacion y aplicaciones.

Country Status (4)

Country Link
EP (1) EP1336653A1 (es)
AU (1) AU2001295630A1 (es)
ES (1) ES2167278B1 (es)
WO (1) WO2002034898A1 (es)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5178762A (en) * 1990-10-18 1993-01-12 The Mead Corporation Soybean peroxidase treatment of contaminated substances
DE69232002T2 (de) * 1991-06-07 2002-06-06 Johnson & Johnson Clin Diag Immunoassays unter Verwendung von markierten Hapten-Analogen
US5391488A (en) * 1993-04-19 1995-02-21 Enzymol International, Inc. Biocatalytic oxidation of a reactive primary alcohol using soybean peroxidase
IT1279043B1 (it) * 1995-05-24 1997-12-04 Co Ri Al Scpa Metodo per la determinazione di acido lattico in materiali organici di interesse alimentare e biosensore per l'esecuzione di tale metodo
US5866695A (en) * 1995-10-27 1999-02-02 Indiana Crop Improvement Association Soybean peroxydase gene family and an assay for detecting soybean peroxidase activity

Also Published As

Publication number Publication date
ES2167278A1 (es) 2002-05-01
EP1336653A1 (en) 2003-08-20
AU2001295630A1 (en) 2002-05-06
WO2002034898A8 (es) 2003-10-16
WO2002034898A1 (es) 2002-05-02

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