ES2156283T3 - Procedimiento de ensayo in vitro para la deteccion de efectos embriotoxicos/teratogenicos inducidos por sustancias quimicas. - Google Patents

Procedimiento de ensayo in vitro para la deteccion de efectos embriotoxicos/teratogenicos inducidos por sustancias quimicas.

Info

Publication number
ES2156283T3
ES2156283T3 ES96922735T ES96922735T ES2156283T3 ES 2156283 T3 ES2156283 T3 ES 2156283T3 ES 96922735 T ES96922735 T ES 96922735T ES 96922735 T ES96922735 T ES 96922735T ES 2156283 T3 ES2156283 T3 ES 2156283T3
Authority
ES
Spain
Prior art keywords
pct
detection
cells
test procedure
differentiation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96922735T
Other languages
English (en)
Inventor
Anna Magdalene Wobus
Wolfgang-Michael Franz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut fuer Pflanzengenetik und Kulturpflanzenforschung
Original Assignee
Institut fuer Pflanzengenetik und Kulturpflanzenforschung
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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Application filed by Institut fuer Pflanzengenetik und Kulturpflanzenforschung filed Critical Institut fuer Pflanzengenetik und Kulturpflanzenforschung
Application granted granted Critical
Publication of ES2156283T3 publication Critical patent/ES2156283T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5073—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5023—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on expression patterns
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5082—Supracellular entities, e.g. tissue, organisms
    • G01N33/5088—Supracellular entities, e.g. tissue, organisms of vertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/142—Toxicological screening, e.g. expression profiles which identify toxicity

Abstract

LA INVENCION TRATA DE UN PROCEDIMIENTO PARA LA REALIZACION DE PRUEBAS IN VITRO CON EL FIN DE DETECTAR EFECTOS INDUCIDOS QUIMICAMENTE EN EL DESARROLLO EMBRIONAL Y PODER HACER UNA DIFERENCIACION EN LA EXPLORACION DE LA EMBRIOTOXICIDAD/TERATOGENETICA, BASADA EN DIFERENTES CELULAS DE CEPA (ES) EMBRIONALES PLURIPOTENTES DE RATON Y RATA UTILIZANDO CELULAS DE GERMENES (EG) EMBRIONALES OBTENIDAS A PARTIR DE CELULAS DE GERMENES PRIMORDIALES. EL PROCEDIMIENTO PARA LA REALIZACION DE PRUEBAS SEGUN LA INVENCION, SE CARACTERIZA PORQUE SE SELECCIONAN UNOS CLONES DE CELULA ES O EG ESTABLES TRANSGENICOS, QUE CONTIENEN UNOS PROMOTORES ESPECIFICOS DEL TEJIDO Y UNOS GENES REPORTER, REALIZANDOSE UNA EXPRESION DEPENDIENTE DE LA DIFERENCIACION DE GENES ESPECIFICOS DEL TEJIDO MEDIANTE LA DIFERENCIACION DE CELULAS ES, EN PRESENCIA DE UNAS SUSTANCIAS EMBRIOTOXICAS QUE ACTUAN EN DETERMINADOS MOMENTOS EN DIFERENTES DERIVADOS DEL RECORRIDO DE GERMINACION Y DETECTANDOSE A CONTINUACION LA ACTIVACION INDUCIDA QUIMICAMENTE, ASI COMO LA REPRESION Y MODULACION DE GENES ESPECIFICOS DEL TEJIDO Y QUE REGULAN EL DESARROLLO EMBRIONAL.
ES96922735T 1995-06-28 1996-06-27 Procedimiento de ensayo in vitro para la deteccion de efectos embriotoxicos/teratogenicos inducidos por sustancias quimicas. Expired - Lifetime ES2156283T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19525285A DE19525285C2 (de) 1995-06-28 1995-06-28 In vitro Testverfahren zum Nachweis Chemikalien-induzierter embryotoxischer/teratogener Effekte

Publications (1)

Publication Number Publication Date
ES2156283T3 true ES2156283T3 (es) 2001-06-16

Family

ID=7766572

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96922735T Expired - Lifetime ES2156283T3 (es) 1995-06-28 1996-06-27 Procedimiento de ensayo in vitro para la deteccion de efectos embriotoxicos/teratogenicos inducidos por sustancias quimicas.

Country Status (7)

Country Link
US (1) US6007993A (es)
EP (1) EP0835325B1 (es)
AT (1) ATE199400T1 (es)
DE (2) DE19525285C2 (es)
DK (1) DK0835325T3 (es)
ES (1) ES2156283T3 (es)
WO (1) WO1997001644A1 (es)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MXPA01005745A (es) * 1998-12-09 2003-07-14 Vistagen Inc Tipificacion de la toxicidad utilizando cuerpos embrioides.
AU2001268449A1 (en) 2000-06-14 2001-12-24 Vistagen, Inc. Toxicity typing using liver stem cells
AU2002241758A1 (en) * 2000-10-26 2002-06-18 University Of Florida Drug screening system
IL160507A0 (en) * 2001-08-24 2004-07-25 Advanced Cell Tech Inc Screening assays for identifying differentiation-inducing agents and production of differentiated cells for cell therapy
EP1641912B1 (en) 2003-06-20 2016-04-27 Axiogenesis Ag Tissue modelling in embryonic stem (es) cell system
ITMI20040467A1 (it) * 2004-03-12 2004-06-12 Istituto Sperimentale Italiano Uso di gameti ed embrioni preimpianto per la determinazione della tossicita' di composti biologicamente attivi
JP4814875B2 (ja) 2004-05-11 2011-11-16 アキシオジェネシス エージー ｉｎｖｉｔｒｏ分化細胞に基づく薬物発見のための検定
US8431397B2 (en) * 2004-09-14 2013-04-30 The Trustees Of Columbia University In The City Of New York Differentiation of human mesenchymal stem cells to cardiac progenitor cells that promote cardiac repair
WO2006032054A2 (en) * 2004-09-14 2006-03-23 The Trustees Of Columbia University In The City Of New York Differentiation of human mesenchymal stem cells to cardiac progenitor cells that promote cardiac repair
CA2613529A1 (en) * 2005-06-22 2007-01-04 Geron Corporation Reporter hepatocytes and other cells for drug screening and toxicity testing
US20060292694A1 (en) * 2005-06-22 2006-12-28 Roslin Institute Reporter hepatocytes and other cells for drug screening and toxicity testing
US20060292695A1 (en) * 2005-06-22 2006-12-28 Roslin Institute Methods and kits for drug screening and toxicity testing using promoter-reporter cells derived from embryonic stem cells
US9155762B2 (en) * 2005-12-08 2015-10-13 University Of Louisville Research Foundation, Inc. Uses and isolation of stem cells from bone marrow
PL2535403T3 (pl) * 2005-12-08 2019-10-31 Univ Louisville Res Found Inc Bardzo małe komórki macierzyste typu embrionalnego (VSEL) oraz sposoby ich izolowania i zastosowania
WO2007084585A2 (en) * 2006-01-19 2007-07-26 Magee-Womans Health Corporation Non-human primate embryonic stem and germ cells: methods of use and methods of making same
GB0911060D0 (en) 2009-06-26 2009-08-12 Ge Healthcare Uk Ltd Methods for predicting the toxicity of a chemical
WO2011115993A2 (en) * 2010-03-15 2011-09-22 The Johns Hopkins University Method for determining substance non-toxicity
JP5814337B2 (ja) * 2010-03-22 2015-11-17 ステミナ バイオマーカー ディスカバリー， インコーポレイテッド ヒト幹細胞様細胞及びメタボロミクスを使用した医薬のヒト発生毒性の予測
RU2685914C1 (ru) 2013-12-11 2019-04-23 Регенерон Фармасьютикалс, Инк. Способы и композиции для направленной модификации генома
WO2021015611A1 (en) * 2019-07-19 2021-01-28 Toxys B.V. Methods to determine the effect of an agent on mammalian embryonic development.

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4744909A (en) * 1987-02-02 1988-05-17 Vertech Treatment Systems, Inc. Method of effecting accelerated oxidation reaction
EP0390857A4 (en) * 1987-12-15 1991-04-17 The Trustees Of Princeton University Transgenic testing systems for mutagens and carcinogens
EP0370813A3 (en) * 1988-11-25 1991-06-19 Exemplar Corporation Rapid screening mutagenesis and teratogenesis assay
US5346812A (en) * 1989-09-07 1994-09-13 The University Of Miami Teratogen assay

Also Published As

Publication number Publication date
DE59606520D1 (de) 2001-04-05
EP0835325B1 (de) 2001-02-28
DE19525285A1 (de) 1997-01-02
WO1997001644A1 (de) 1997-01-16
DE19525285C2 (de) 1999-04-15
DK0835325T3 (da) 2001-07-16
ATE199400T1 (de) 2001-03-15
US6007993A (en) 1999-12-28
EP0835325A1 (de) 1998-04-15

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