ES2152337T3 - Metodo para detectar biomoleculas por medio de un sistema de amplificacion enzimatica. - Google Patents

Metodo para detectar biomoleculas por medio de un sistema de amplificacion enzimatica.

Info

Publication number
ES2152337T3
ES2152337T3 ES95103225T ES95103225T ES2152337T3 ES 2152337 T3 ES2152337 T3 ES 2152337T3 ES 95103225 T ES95103225 T ES 95103225T ES 95103225 T ES95103225 T ES 95103225T ES 2152337 T3 ES2152337 T3 ES 2152337T3
Authority
ES
Spain
Prior art keywords
enzymatic amplification
amplification system
semi
detecting biomolecules
biomolecules
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95103225T
Other languages
English (en)
Inventor
Adelio Rigo
Giuliana Bernacca
Ciana Leopoldo Della
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ministero dell Universita e della Ricerca Scientifica e Tecnologica (MURST)
Original Assignee
Ministero dell Universita e della Ricerca Scientifica e Tecnologica (MURST)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ministero dell Universita e della Ricerca Scientifica e Tecnologica (MURST) filed Critical Ministero dell Universita e della Ricerca Scientifica e Tecnologica (MURST)
Application granted granted Critical
Publication of ES2152337T3 publication Critical patent/ES2152337T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Emergency Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

UNAS BIOMOLECULAS TALES COMO ANTICUERPOS, ANTIGENOS O ADN, PUEDEN MEDIRSE EN CONCENTRACIONES PARTICULARMENTE BAJAS MEDIANTE AMPEROMETRIA CON EL USO DE UNA ENZIMA MARCADORA TAL COMO FOSFATASA ALCALINA JUNTO CON UNA NUEVA CLASE DE SUSTRATOS DE FOSFATO HIDROXIARIL PARA- U ORTO- DE FORMULA GENERAL (I), EN LA QUE AR ES FENIL O NAFTIL POSIBLEMENTE SUSTITUIDOS, QUE PROPORCIONAN UN INCREMENTO DE QUINONAS MEDIANTE LA OXIDACION EN EL ELECTRODO. ESTE SISTEMA PUEDE UTILIZARSE JUNTO CON UN SEGUNDO SISTEMA DE AMPLIFICACION ENZIMATICO CONSTITUIDO POR EL PROPIO ELECTRODO DE MEDICION Y UN SISTEMA DE OXIDASA GLUCOSA-GLUCOSA QUE POSIBILITA QUE LOS LIMITES DE SENSITIVIDAD DE LAS MEDICIONES BIOMOLECULARES SEAN REDUCIDOS, O LOS TIEMPOS DE DETERMINACION SEAN REDUCIDOS CONSIDERABLEMENTE.
ES95103225T 1994-03-11 1995-03-07 Metodo para detectar biomoleculas por medio de un sistema de amplificacion enzimatica. Expired - Lifetime ES2152337T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT94TO000177A IT1267410B1 (it) 1994-03-11 1994-03-11 Procedimento per la determinazione di biomolecole mediante un sistema di amplificazione enzimatica.

Publications (1)

Publication Number Publication Date
ES2152337T3 true ES2152337T3 (es) 2001-02-01

Family

ID=11412296

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95103225T Expired - Lifetime ES2152337T3 (es) 1994-03-11 1995-03-07 Metodo para detectar biomoleculas por medio de un sistema de amplificacion enzimatica.

Country Status (4)

Country Link
EP (1) EP0671625B1 (es)
DE (1) DE69518995T2 (es)
ES (1) ES2152337T3 (es)
IT (1) IT1267410B1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2805545B1 (fr) * 2000-02-24 2002-05-17 Argene Sa Procede electrochimique de detection d'acides nucleiques
DE102018200262A1 (de) 2018-01-10 2019-07-11 Robert Bosch Gmbh Biomolekulares Detektionsverfahren

Also Published As

Publication number Publication date
EP0671625A1 (en) 1995-09-13
DE69518995D1 (de) 2000-11-09
EP0671625B1 (en) 2000-10-04
ITTO940177A0 (it) 1994-03-11
IT1267410B1 (it) 1997-02-05
ITTO940177A1 (it) 1995-09-11
DE69518995T2 (de) 2001-03-22

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