ES2150407T3 - Secuenciacion multiple. - Google Patents

Secuenciacion multiple.

Info

Publication number
ES2150407T3
ES2150407T3 ES88307391T ES88307391T ES2150407T3 ES 2150407 T3 ES2150407 T3 ES 2150407T3 ES 88307391 T ES88307391 T ES 88307391T ES 88307391 T ES88307391 T ES 88307391T ES 2150407 T3 ES2150407 T3 ES 2150407T3
Authority
ES
Spain
Prior art keywords
tag sequence
fragments
dna
vector
bases
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES88307391T
Other languages
English (en)
Inventor
George M Church
Stephen Kieffer-Higgins
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Harvard College
Original Assignee
Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard College filed Critical Harvard College
Application granted granted Critical
Publication of ES2150407T3 publication Critical patent/ES2150407T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/803—Physical recovery methods, e.g. chromatography, grinding

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Diaphragms For Electromechanical Transducers (AREA)
  • Laminated Bodies (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

ESTA INVENCION CARACTERIZA VECTORES Y UN METODO PARA FORMAR SECUENCIAS DE ADN. EL METODO INCLUYE LOS PASOS DE: A)LIGAR EL ADN A UN VECTOR QUE COMPRENDE UNA SECUENCIA DE MARCADO, LA SECUENCIA DE MARCADO INCLUYE AL MENOS 15 BASES, Y LA SECUENCIA DE MARCADO NO HIBRIDIZARA AL ADN BAJO CONDICIONES DE HIBRIDACION ESTRICTAS Y ES UNICA EN EL VECTOR PARA FORMAR UN VECTOR HIBRIDO B)TRATAR EL VECTOR HIBRIDO EN UNA PLURALIDAD DE RECIPIENTES PARA PRODUCIR FRAGMENTOS QUE COMPRENDEN LA SECUENCIA DE MARCADO, Y LOS FRAGMENTOS DIFIEREN EN LONGITUD Y TERMINAN EN UNA BASE O BASES FIJAS Y LA BASE FIJA CONOCIDA (O BASES) DIFIEREN EN CADA RECIPIENTE C)SEPARAR LOS FRAGMENTOS DE CADA RECIPIENTE SEGUN SU TAMAÑO D)HIBRIDIZAR LOS FRAGMENTOS CON UN OLIGONUCLEOTIDO CAPAZ DE HIBRIDIZAR ESPECIFICAMENTE CON LA SECUENCIA DE MARCADO Y, E)DETECTAR EL MODELO DE HIBRIDACION DE LA SECUENCIA DE MERCADO, Y EL MODELO REFLEJA LA SECUENCIA NUCLEOTIDA DEL ADN.
ES88307391T 1987-08-11 1988-08-10 Secuenciacion multiple. Expired - Lifetime ES2150407T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8462387A 1987-08-11 1987-08-11
US07/228,596 US4942124A (en) 1987-08-11 1988-08-04 Multiplex sequencing

Publications (1)

Publication Number Publication Date
ES2150407T3 true ES2150407T3 (es) 2000-12-01

Family

ID=26771208

Family Applications (1)

Application Number Title Priority Date Filing Date
ES88307391T Expired - Lifetime ES2150407T3 (es) 1987-08-11 1988-08-10 Secuenciacion multiple.

Country Status (7)

Country Link
US (1) US4942124A (es)
EP (1) EP0303459B1 (es)
JP (1) JP2665775B2 (es)
AT (1) ATE196656T1 (es)
CA (1) CA1339727C (es)
DE (1) DE3856427T2 (es)
ES (1) ES2150407T3 (es)

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* Cited by examiner, † Cited by third party
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EP0303459A2 (en) 1989-02-15
EP0303459B1 (en) 2000-09-27
JP2665775B2 (ja) 1997-10-22
JPH01137982A (ja) 1989-05-30
CA1339727C (en) 1998-03-17
EP0303459A3 (en) 1990-01-17
DE3856427T2 (de) 2001-02-08
US4942124A (en) 1990-07-17
ATE196656T1 (de) 2000-10-15
DE3856427D1 (de) 2000-11-02

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