ES2146666T3 - Procedimiento para la obtencion recombinante de proteinas en levadura. - Google Patents

Procedimiento para la obtencion recombinante de proteinas en levadura.

Info

Publication number
ES2146666T3
ES2146666T3 ES94929554T ES94929554T ES2146666T3 ES 2146666 T3 ES2146666 T3 ES 2146666T3 ES 94929554 T ES94929554 T ES 94929554T ES 94929554 T ES94929554 T ES 94929554T ES 2146666 T3 ES2146666 T3 ES 2146666T3
Authority
ES
Spain
Prior art keywords
procedure
yeast
yeast proteins
recombinant
obtaining
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94929554T
Other languages
English (en)
Inventor
Jurgen Schweden
Claus Bollschweiler
Michael Piontek
Ulrike Weydemann
Gerd Gellissen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF SE
Dynavax GmbH
Original Assignee
BASF SE
Rhein Biotech GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BASF SE, Rhein Biotech GmbH filed Critical BASF SE
Application granted granted Critical
Publication of ES2146666T3 publication Critical patent/ES2146666T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PROCEDIMIENTO PARA LA OBTENCION DE RECOMBINANTES DE LEVADURA CARACTERIZADO PORQUE LA LEVADURA SE TRANSFORMA CON UNA SECUENCIA DE EXPRESION QUE LLEVA LOS SIGUIENTES COMPONENTES ESTRUCTURALES CODIFICADOS: L-A-P-GEN, DONDE L ES UNA SECUENCIA DE UNA NEUROHORMONA PEPTIDICA ANIMAL, A ES UN ADAPTADOR QUE GENERA UNA ESTRUCTURA EN ALFA-HELICE, P ES UNA SEÑAL DE PROCESAMIENTO Y GEN ES UN GEN ESTRUCTURAL PARA LA PROTEINA DESEADA.
ES94929554T 1993-10-28 1994-10-17 Procedimiento para la obtencion recombinante de proteinas en levadura. Expired - Lifetime ES2146666T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE4336810A DE4336810A1 (de) 1993-10-28 1993-10-28 Verfahren zur rekombinanten Herstellung von Proteinen in Hefe

Publications (1)

Publication Number Publication Date
ES2146666T3 true ES2146666T3 (es) 2000-08-16

Family

ID=6501242

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94929554T Expired - Lifetime ES2146666T3 (es) 1993-10-28 1994-10-17 Procedimiento para la obtencion recombinante de proteinas en levadura.

Country Status (9)

Country Link
US (1) US5672487A (es)
EP (1) EP0725822B1 (es)
JP (1) JPH09503919A (es)
AT (1) ATE192780T1 (es)
DE (2) DE4336810A1 (es)
DK (1) DK0725822T3 (es)
ES (1) ES2146666T3 (es)
PT (1) PT725822E (es)
WO (1) WO1995011976A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6890730B1 (en) 1999-12-10 2005-05-10 The Salk Institute Sequence and method for increasing protein expression in cellular expression systems
ATE513046T1 (de) 2001-03-09 2011-07-15 Univ Kentucky Res Found Cytochrom p450s und verwendungen davon
US20060234351A1 (en) * 2002-09-13 2006-10-19 Sahib Maharaj K Yeast protein expression secretion system

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK172738B1 (da) * 1983-04-07 1999-06-21 Chiron Corp Fremgangsmåde til at udtrykke modent insulin og DNA-struktur til anvendelse ved fremgangsmåden
JP2535066B2 (ja) * 1987-03-20 1996-09-18 クリエイティブ・バイオマリキュールズ・インコーポレーテッド 組換え体タンパク質製造のためのリ―ダ―配列

Also Published As

Publication number Publication date
ATE192780T1 (de) 2000-05-15
WO1995011976A1 (de) 1995-05-04
EP0725822A1 (de) 1996-08-14
US5672487A (en) 1997-09-30
DK0725822T3 (da) 2000-12-04
JPH09503919A (ja) 1997-04-22
DE59409341D1 (de) 2000-06-15
EP0725822B1 (de) 2000-05-10
DE4336810A1 (de) 1995-05-18
PT725822E (pt) 2000-11-30

Similar Documents

Publication Publication Date Title
IE883612L (en) Vectors and compounds for direct expression of activated human protein c
NZ232742A (en) Yeast heterologous protein production method, vectors, dna constructs and proteins so produced and aprotinin (1-58) so produced
AU1575088A (en) Process for the purification of recombinant polypeptides
BR9609325A (pt) Uso de um polipeptídeo ou proteína com sequência de aminoácidos processos para preparar um produto melhorado e para produzir polipeptídeos ou proteínas recombinantes organismo hospedeiro recombinante de tipo alimentício produto e polipeptídeo ou proteína de tipo alimentício recombinante substancialmente puro ou isolado
AU7149691A (en) Process for enzymatical cleavage of recombinant proteins by means of iga proteases
AU5053796A (en) Cytoplasmic expression of antibodies, antibody fragments and antibody fragment fusion molecules in E. Coli
FI865003A (fi) Menetelmä trombiini-inhibiittorien tuottamiseksi
AU8110487A (en) DNA molecules coding for proteins targeted to plant cell walls
CA2260365A1 (en) Differentiation-suppressive polypeptide
EP0494702A3 (en) Production of factor xiiia by gene technology
AU7873798A (en) N-terminally extended proteins expressed in yeast
DE3881023D1 (de) Proteine, expressionsvektoren, die fuer solche proteine kodierende dna enthalten und zellen, die solche expressionsvektoren enthalten.
ES2146666T3 (es) Procedimiento para la obtencion recombinante de proteinas en levadura.
DE3877035D1 (de) Gentechnologisches verfahren zur herstellung von polypeptiden.
AU7515491A (en) Process for producing foreign proteins in streptomyces
ES2145838T3 (es) Procedimiento para la obtencion recombinante de proteinas en la levadura hansenula.
PE42695A1 (es) Procedimiento para producir proteina c
CA2025350A1 (en) Process for production of c-terminal .alpha.-amidated peptide
SE9504019D0 (sv) Method for production of proteins
KR890008166A (ko) 융합 단백질의 선택적 분해 방법
HU912398D0 (en) An in-vitro process for fusion of proteins
CA2175068A1 (en) The recombinant production of proteins in yeast
FI870501A (fi) Menetelmä melanoomaan liittyvän p97-antigeenin antigeenisen sukulaispeptidin tai -proteiinin valmistamiseksi, sekä siinä käyttökelpoiset yhdistelmävirukset, yhdistelmävektorit ja isäntäsolut
AU654155B2 (en) A DNA encoding a protein which binds to the enhancer of alpha-fetoprotein gene
GR890100542A (el) Συστηματα εκφρασεως για την παρασκευη πολυπεπτιδιων σε προκαρυοτικα κυτταρα.

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 725822

Country of ref document: ES