ES2133503T3 - Dosificacion inmunologica de un antigeno o un hapteno. - Google Patents
Dosificacion inmunologica de un antigeno o un hapteno.Info
- Publication number
- ES2133503T3 ES2133503T3 ES94400166T ES94400166T ES2133503T3 ES 2133503 T3 ES2133503 T3 ES 2133503T3 ES 94400166 T ES94400166 T ES 94400166T ES 94400166 T ES94400166 T ES 94400166T ES 2133503 T3 ES2133503 T3 ES 2133503T3
- Authority
- ES
- Spain
- Prior art keywords
- antigen
- hapten
- antibodies
- epitope
- antibody
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/815—Test for named compound or class of compounds
- Y10S436/817—Steroids or hormones
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/822—Identified hapten
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
LA INVENCION SE REFIERE A UN PROCEDIMIENTO DE DOSIFICACION INMUNOMETRICA DE UN ANTIGENO O DE UN HAPTENO. CONFORME AL PROCEDIMIENTO, SE PONE EN CONTACTO (FIGURA 1A) EL ANTIGENO O EL HAPTENO (3) A DOSIFICAR CON ANTICUERPOS (2) FIJOS SOBRE UNA FASE SOLIDA, PARA ENLAZAR INMUNOLOGICAMENTE EL ANTIGENO O EL HAPTENO CON EL ANTICUERPO. A CONTINUACION SE REALIZA (FIGURA 1B) UN TRATAMIENTO DE INMOVILIZACION DEL ANTIGENO O DEL HAPTENO (3) MEDIANTE ENLACE COVALENTE (4) SOBRE LA FASE SOLIDA (1), LIBERANDO SU EPITOPE (FIGURA 1C); DESPUES SE LE PONE EN CONTACTO (FIGURA 1D) CON ANTICUERPOS MARCADOS (5) Y SE DETERMINA (FIGURA 1E) LA CANTIDAD DE ANTICUERPOS MARCADOS FIJOS, PARA DEDUCIR DE ELLO LA CONCENTRACION DE ANTIGENO O HAPTENO DE PARTIDA. EN VIRTUD DE ESTA ETAPA DE INMOVILIZACION Y DE LIBERACION DEL EPITOPE, SE OBTIENE UNA SENSIBILIDAD ELEVADA UTILIZANDO SOLAMENTE UN ANTICUERPO.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR9300869A FR2700855B1 (fr) | 1993-01-28 | 1993-01-28 | Dosage immunométrique d'un antigène ou d'un haptène. |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2133503T3 true ES2133503T3 (es) | 1999-09-16 |
Family
ID=9443449
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES94400166T Expired - Lifetime ES2133503T3 (es) | 1993-01-28 | 1994-01-26 | Dosificacion inmunologica de un antigeno o un hapteno. |
Country Status (7)
Country | Link |
---|---|
US (1) | US5476770A (es) |
EP (1) | EP0609144B1 (es) |
JP (1) | JP3310090B2 (es) |
CA (1) | CA2113383C (es) |
DE (1) | DE69418501T2 (es) |
ES (1) | ES2133503T3 (es) |
FR (1) | FR2700855B1 (es) |
Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5688506A (en) * | 1994-01-27 | 1997-11-18 | Aphton Corp. | Immunogens against gonadotropin releasing hormone |
US5619955A (en) * | 1995-06-29 | 1997-04-15 | Stone Products, Inc. | Harness gripping aid for tandem riders |
FI100276B (fi) * | 1996-02-06 | 1997-10-31 | Orion Yhtymae Oy | Menetelmä analyyttien ei-kilpailevaa määritystä varten |
FR2799839A1 (fr) * | 2000-02-02 | 2001-04-20 | Commissariat Energie Atomique | Procede pour determiner la capacite antioxydante de composes chimiques et procedes de dosage et d'immobilisation utilisables pour cette determination |
US6769347B1 (en) | 2002-11-26 | 2004-08-03 | Recon/Optical, Inc. | Dual elevation weapon station and method of use |
US7429472B2 (en) | 2003-01-31 | 2008-09-30 | Promega Corporation | Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule |
KR20050109934A (ko) | 2003-01-31 | 2005-11-22 | 프로메가 코포레이션 | 관능기의 단백질로의 공유 테더링 |
FR2853965B1 (fr) * | 2003-04-15 | 2006-09-15 | Commissariat Energie Atomique | Procede de criblage de conditions operatoires d'une reaction chimique de couplage et trousses pour la mise en oeuvre de ce procede |
US20050227351A1 (en) * | 2004-04-08 | 2005-10-13 | Awdalla Essam T | Methods and compound protein molecules used for inducing cytotoxic cell-mediated immune response in a mammal against a pathogenic cell |
US20070087400A1 (en) * | 2004-07-30 | 2007-04-19 | Aldis Darzins | Covalent tethering of functional groups to proteins and substrates therefor |
US7425436B2 (en) | 2004-07-30 | 2008-09-16 | Promega Corporation | Covalent tethering of functional groups to proteins and substrates therefor |
EP1989548A2 (en) * | 2006-02-08 | 2008-11-12 | Promega Corporation | Compositions and methods for capturing and analyzing cross-linked biomolecules |
US8420367B2 (en) | 2006-10-30 | 2013-04-16 | Promega Corporation | Polynucleotides encoding mutant hydrolase proteins with enhanced kinetics and functional expression |
DE102007045099A1 (de) * | 2007-09-20 | 2008-10-30 | Dade Behring Marburg Gmbh | Verfahren zur Bestimmung eines Analyten mittels Ultraschall |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4376110A (en) * | 1980-08-04 | 1983-03-08 | Hybritech, Incorporated | Immunometric assays using monoclonal antibodies |
US4459359A (en) * | 1981-11-19 | 1984-07-10 | New York Blood Center, Inc. | Sensitive immunoassays of antigens or antibodies sequestered within immune complexes |
US4703001A (en) * | 1985-10-23 | 1987-10-27 | Synbiotics, Corporation | Immunoassay for the detection of serum analytes using pH dependent chastropic acids |
US4794090A (en) * | 1986-09-26 | 1988-12-27 | W. R. Grace & Co.-Conn. | Immobilization support for biologicals |
US5236830A (en) * | 1988-11-10 | 1993-08-17 | Eiji Ishikawa | Method of assay for antigen |
DK100490D0 (da) * | 1990-04-24 | 1990-04-24 | Novo Nordisk As | Overflademodifikation |
ES2089057T3 (es) * | 1990-07-18 | 1996-10-01 | Abbott Lab | Un reactivo sustituto de un analito para uso en metodos de ensayo de fijacion especifica, dispositivos y kits. |
-
1993
- 1993-01-28 FR FR9300869A patent/FR2700855B1/fr not_active Expired - Fee Related
-
1994
- 1994-01-13 CA CA002113383A patent/CA2113383C/en not_active Expired - Fee Related
- 1994-01-24 US US08/184,935 patent/US5476770A/en not_active Expired - Lifetime
- 1994-01-26 DE DE69418501T patent/DE69418501T2/de not_active Expired - Fee Related
- 1994-01-26 ES ES94400166T patent/ES2133503T3/es not_active Expired - Lifetime
- 1994-01-26 EP EP94400166A patent/EP0609144B1/fr not_active Expired - Lifetime
- 1994-01-28 JP JP00885894A patent/JP3310090B2/ja not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
EP0609144A1 (fr) | 1994-08-03 |
FR2700855A1 (fr) | 1994-07-29 |
US5476770A (en) | 1995-12-19 |
JP3310090B2 (ja) | 2002-07-29 |
DE69418501D1 (de) | 1999-06-24 |
JPH06273416A (ja) | 1994-09-30 |
CA2113383C (en) | 2007-04-24 |
DE69418501T2 (de) | 1999-12-16 |
EP0609144B1 (fr) | 1999-05-19 |
FR2700855B1 (fr) | 1995-03-03 |
CA2113383A1 (en) | 1994-07-29 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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