ES2122257T3 - Proteinas de fusion de la toxina del antrax y usos de las mismas. - Google Patents

Proteinas de fusion de la toxina del antrax y usos de las mismas.

Info

Publication number
ES2122257T3
ES2122257T3 ES94911385T ES94911385T ES2122257T3 ES 2122257 T3 ES2122257 T3 ES 2122257T3 ES 94911385 T ES94911385 T ES 94911385T ES 94911385 T ES94911385 T ES 94911385T ES 2122257 T3 ES2122257 T3 ES 2122257T3
Authority
ES
Spain
Prior art keywords
protein
domain
cell
native
binding domain
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94911385T
Other languages
English (en)
Inventor
Stephen H Leppla
Kurt Klimpel
Naveen Arora
Yogendra Singh
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
US Department of Health and Human Services
Original Assignee
US Department of Health and Human Services
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/021,601 external-priority patent/US5591631A/en
Application filed by US Department of Health and Human Services filed Critical US Department of Health and Human Services
Application granted granted Critical
Publication of ES2122257T3 publication Critical patent/ES2122257T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/62—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
    • A61K47/64—Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
    • A61K47/6425—Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent the peptide or protein in the drug conjugate being a receptor, e.g. CD4, a cell surface antigen, i.e. not a peptide ligand targeting the antigen, or a cell surface determinant, i.e. a part of the surface of a cell
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/21—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pseudomonadaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/77—Internalization into the cell
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/55—Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16211—Human Immunodeficiency Virus, HIV concerning HIV gagpol
    • C12N2740/16222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Microbiology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

EN LA PRESENTE INVENCION SE PRESENTA UN ACIDO NUCLEICO QUE CODIFICA UNA PROTEINA DE FUSION QUE CONTIENE UNA SECUENCIA DE NUCLEOTIDOS QUE CODIFICA EL DOMINO DE ENLACE DEL ANTIGENO PROTECTOR (PA) DE ANTRAX DE LA PROTEINA DE FACTOR LETAL (LF) DE ANTRAX NATIVA Y UNA SECUENCIA DE NUCLEOTIDOS QUE CODIFICA UN DOMINIO INDUCTOR DE UNA ACTIVIDAD DE UNA SEGUNDA PROTEINA. TAMBIEN SE PRESENTA UN ACIDO NUCLEICO QUE CODIFICA UNA PROTEINA DE FUSION QUE CONTIENE UNA SECUENCIA DE NUCLEOTIDOS QUE CODIFICA EL DOMINIO DE TRANSUBICACION Y L DOMINIO DE ENLACE DE LF DE LA PROTEINA DEL PA DE ANTRAX NATIVA Y UNA SECUENCIA DE NUCLEOTIDOS QUE CODIFICA UN DOMINIO DE LIGANDOS QUE ESPECIFICAMENTE SE UNE A UN OBJETIVO CELULAR. TAMBIEN SE PRESENTAN LAS PROTEINAS CODIFICADAS POR EL ACIDO NUCLEICO DE LA INVENCION, ASI COMO UN METODO PARA PROPORCIONAR UNA ACTIVIDAD A UNA CELULA UTILIZANDO TALES PROTEINAS DE FUSION. EN LA INVENCION TAMBIEN SE PRESENTAN PROTEINAS QUE INCLUYEN UN ANTIGENO PROTECTOR DE ANTRAX QUE SE HA MUTADO PARA SUSTITUIR EL LUGAR DE LIXIVIACION DE TRIPSINA POR RESIDUOS RECONOCIDOS ESPECIFICAMENTE POR LA PROTEASA DEL VIH-1.
ES94911385T 1993-02-12 1994-02-14 Proteinas de fusion de la toxina del antrax y usos de las mismas. Expired - Lifetime ES2122257T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/021,601 US5591631A (en) 1993-02-12 1993-02-12 Anthrax toxin fusion proteins, nucleic acid encoding same
US08/082,849 US5677274A (en) 1993-02-12 1993-06-25 Anthrax toxin fusion proteins and related methods

Publications (1)

Publication Number Publication Date
ES2122257T3 true ES2122257T3 (es) 1998-12-16

Family

ID=26694882

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94911385T Expired - Lifetime ES2122257T3 (es) 1993-02-12 1994-02-14 Proteinas de fusion de la toxina del antrax y usos de las mismas.

Country Status (8)

Country Link
US (1) US5677274A (es)
EP (1) EP0684997B1 (es)
AT (1) ATE169959T1 (es)
AU (1) AU682500B2 (es)
CA (1) CA2155514A1 (es)
DE (1) DE69412593T2 (es)
ES (1) ES2122257T3 (es)
WO (1) WO1994018332A2 (es)

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Also Published As

Publication number Publication date
DE69412593D1 (de) 1998-09-24
DE69412593T2 (de) 1999-02-18
AU6392294A (en) 1994-08-29
US5677274A (en) 1997-10-14
AU682500B2 (en) 1997-10-09
WO1994018332A2 (en) 1994-08-18
WO1994018332A3 (en) 1994-10-13
EP0684997B1 (en) 1998-08-19
ATE169959T1 (de) 1998-09-15
EP0684997A1 (en) 1995-12-06
CA2155514A1 (en) 1994-08-18

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