ES2117203T5 - Procedimiento de produccion de enzima dextranasa y decodificacion de su adn. - Google Patents
Procedimiento de produccion de enzima dextranasa y decodificacion de su adn.Info
- Publication number
- ES2117203T5 ES2117203T5 ES94203614T ES94203614T ES2117203T5 ES 2117203 T5 ES2117203 T5 ES 2117203T5 ES 94203614 T ES94203614 T ES 94203614T ES 94203614 T ES94203614 T ES 94203614T ES 2117203 T5 ES2117203 T5 ES 2117203T5
- Authority
- ES
- Spain
- Prior art keywords
- dextranase
- decodification
- dna
- procedure
- enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108010001682 Dextranase Proteins 0.000 title abstract 5
- 238000004519 manufacturing process Methods 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 241001136556 Talaromyces minioluteus Species 0.000 abstract 2
- 241000235058 Komagataella pastoris Species 0.000 abstract 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract 1
- 239000002299 complementary DNA Substances 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
- 108020004999 messenger RNA Proteins 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
- C12N9/2454—Dextranase (3.2.1.11)
Abstract
UN METODO PARA EL AISLAMIENTO Y LA EXPRESION DE UN GEN QUE CODIFICA LA DEXTRANASA DEL HONGO PENICILLIUM MINIOLUTEUM. ESTA ENZIMA DE ORIGEN FUNGICO SE PRODUCE POR EXPRESION EN ALTOS NIVELES DE LEVADURA. PARA ESTE PROPOSITO, SE AISLA Y SE SECUENCIA UNA COPIA DE ADNC DEL ARNM QUE CODIFICA LA ENZIMA DE DEXTRANASA DEL HONGO PENICILLIUM MINIOLUTEUM . ESTE ADNC SE TRANSFIERE A CELULAS DE PICHIA PASTORIS. SE HAN OBTENIDO LEVADURAS RECOMBINANTES CAPACES DE SEGREGAR LA DEXTRANASA AL MEDIO DE CULTIVO. LA ENZIMA DE DEXTRANASA OBTENIDA PUEDE USARSE, POR EJEMPLO, EN LA INDUSTRIA AZUCARERA PARA HIDROLIZAR EL DEXTRANO EN JUGOS DE CAÑA PARA INCREMENTAR LA PRODUCCION DE AZUCAR.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CU1993115 | 1993-12-14 |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2117203T3 ES2117203T3 (es) | 1998-08-01 |
ES2117203T5 true ES2117203T5 (es) | 2001-08-16 |
Family
ID=5459391
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES94203614T Expired - Lifetime ES2117203T5 (es) | 1993-12-14 | 1994-12-13 | Procedimiento de produccion de enzima dextranasa y decodificacion de su adn. |
Country Status (10)
Country | Link |
---|---|
US (1) | US5637491A (es) |
EP (1) | EP0663443B2 (es) |
AT (1) | ATE164627T1 (es) |
AU (1) | AU685181B2 (es) |
BR (1) | BR9404998A (es) |
CO (1) | CO4440441A1 (es) |
DE (1) | DE69409354T3 (es) |
DK (1) | DK0663443T4 (es) |
ES (1) | ES2117203T5 (es) |
ZA (1) | ZA949799B (es) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU685181B2 (en) * | 1993-12-14 | 1998-01-15 | Centro De Ingenieria Genetica Y Biotecnologia | Dextranase enzyme, method for its production and DNA encoding the enzyme |
ES2174248T3 (es) * | 1996-04-16 | 2002-11-01 | Novozymes As | Composiciones para la eliminacion de una placa dental. |
AU719536B2 (en) * | 1996-06-28 | 2000-05-11 | Novozymes A/S | A recombinant enzyme with dextranase activitiy |
KR100604401B1 (ko) * | 2004-01-30 | 2006-07-25 | 주식회사 라이프엔자 | 아밀로펙틴, 전분, 글리코겐 및 아밀로즈 분해활성을 갖는단백질, 그 단백질을 코딩하는 유전자, 그 발현 세포 및상기 단백질의 생산 방법 |
KR20050078077A (ko) * | 2004-01-30 | 2005-08-04 | 주식회사 라이프엔자 | 뮤탠, 이눌린 및 레반을 분해하는 단백질, 그 단백질을코딩하는 유전자, 그 발현 세포 및 상기 단백질의 생산 방법 |
JPWO2004075621A1 (ja) * | 2004-03-11 | 2007-08-23 | 株式会社資生堂 | 抗老化剤およびコラーゲン産生促進剤 |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU685181B2 (en) * | 1993-12-14 | 1998-01-15 | Centro De Ingenieria Genetica Y Biotecnologia | Dextranase enzyme, method for its production and DNA encoding the enzyme |
-
1994
- 1994-12-07 AU AU80274/94A patent/AU685181B2/en not_active Ceased
- 1994-12-08 ZA ZA949799A patent/ZA949799B/xx unknown
- 1994-12-13 ES ES94203614T patent/ES2117203T5/es not_active Expired - Lifetime
- 1994-12-13 DK DK94203614T patent/DK0663443T4/da active
- 1994-12-13 EP EP94203614A patent/EP0663443B2/en not_active Expired - Lifetime
- 1994-12-13 DE DE69409354T patent/DE69409354T3/de not_active Expired - Fee Related
- 1994-12-13 AT AT94203614T patent/ATE164627T1/de not_active IP Right Cessation
- 1994-12-13 US US08/354,618 patent/US5637491A/en not_active Expired - Fee Related
- 1994-12-14 BR BR9404998A patent/BR9404998A/pt not_active IP Right Cessation
- 1994-12-14 CO CO94056588A patent/CO4440441A1/es unknown
Also Published As
Publication number | Publication date |
---|---|
ES2117203T3 (es) | 1998-08-01 |
AU685181B2 (en) | 1998-01-15 |
EP0663443A1 (en) | 1995-07-19 |
DE69409354T2 (de) | 1998-07-23 |
DK0663443T3 (da) | 1998-12-21 |
DK0663443T4 (da) | 2001-12-17 |
BR9404998A (pt) | 1995-08-08 |
AU8027494A (en) | 1995-06-22 |
CO4440441A1 (es) | 1997-05-07 |
EP0663443B1 (en) | 1998-04-01 |
ATE164627T1 (de) | 1998-04-15 |
DE69409354T3 (de) | 2001-06-07 |
ZA949799B (en) | 1996-02-05 |
DE69409354D1 (de) | 1998-05-07 |
US5637491A (en) | 1997-06-10 |
EP0663443B2 (en) | 2001-03-21 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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