ES2117067T3 - Procedimiento para la determinacion inmunoquimica de un analito. - Google Patents
Procedimiento para la determinacion inmunoquimica de un analito.Info
- Publication number
- ES2117067T3 ES2117067T3 ES93107931T ES93107931T ES2117067T3 ES 2117067 T3 ES2117067 T3 ES 2117067T3 ES 93107931 T ES93107931 T ES 93107931T ES 93107931 T ES93107931 T ES 93107931T ES 2117067 T3 ES2117067 T3 ES 2117067T3
- Authority
- ES
- Spain
- Prior art keywords
- analyte
- specific binding
- binding component
- procedure
- immunochemical determination
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/962—Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PROCEDIMIENTO PARA DETERMINAR INMUNOQUIMICAMENTE UN ANALITO EN UNA MUESTRA MEDIANTE UN PRIMER ASOCIADO DE UNION ESPECIFICO DONDE SE INMOVILIZA AL LIGANDO DE UNION ESPECIFICO EN UN PORTADOR Y SE DETERMINA EL GRADO DE UNION DEL ANALITO A LOS ESPECIFICOS LIGANDOS DE UNION MEDIANTE UN SEGUNDO LIGANDO DE UNION ESPECIFICO QUE PORTA DIRECTAMENTE O INDIRECTAMENTE UNA MARCA. SE CARACTERIZA PORQUE AL PROCESO SE LE AÑADE UN FACTOR DE UNION QUE MUESTRA MAS UN LUGAR DE UNION PARA EL AGENTE QUE SE VA A ANALIZAR, DICHO AGENTE QUE NO ES MARCADO Y NO PRESENTA NINGUNA AFINIDAD CON EL LIGANDO DE UNION ESPECIFICO INMOVILIZADO.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE4218257A DE4218257A1 (de) | 1992-06-03 | 1992-06-03 | Verfahren zur immunchemischen Bestimmung eines Analyten |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2117067T3 true ES2117067T3 (es) | 1998-08-01 |
Family
ID=6460278
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES93107931T Expired - Lifetime ES2117067T3 (es) | 1992-06-03 | 1993-05-14 | Procedimiento para la determinacion inmunoquimica de un analito. |
Country Status (8)
Country | Link |
---|---|
US (1) | US5914243A (es) |
EP (1) | EP0572845B1 (es) |
JP (1) | JP3327488B2 (es) |
AT (1) | ATE164682T1 (es) |
AU (1) | AU668937B2 (es) |
CA (1) | CA2097545C (es) |
DE (2) | DE4218257A1 (es) |
ES (1) | ES2117067T3 (es) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE4309393A1 (de) * | 1993-03-23 | 1994-09-29 | Boehringer Mannheim Gmbh | Verringerung des Hook-Effekts in Immuntests mit teilchenförmigem Trägermaterial |
DE19629444A1 (de) * | 1996-07-22 | 1998-01-29 | Behringwerke Ag | Sensitivitätssteigerung bei der immunchemischen Bestimmung eines Analyten |
DE59712575D1 (de) * | 1996-12-19 | 2006-04-20 | Dade Behring Marburg Gmbh | Immundissoziation zur Verbesserung der immunchemischen Bestimmung eines Analyten |
ES2180241T3 (es) | 1998-05-06 | 2003-02-01 | Roche Diagnostics Gmbh | Eliminacion de interferencias causadas por factores reumaticos. |
GB0027064D0 (en) * | 2000-11-06 | 2000-12-20 | Randox Lab Ltd | Multi-analyte immunoassay |
US20040049351A1 (en) * | 2002-08-28 | 2004-03-11 | Matson Robert S. | Immunosorbent assay in microarray format |
DE102004052729A1 (de) | 2004-10-30 | 2006-05-04 | Roche Diagnostics Gmbh | Immunkomplex-spezifsicher Antikörper zur Reduktion des Nullwerts beim Nachweis von Antigen-spezifisch gebundenen Antikörpern einer bestimmten Immunglobulinklasse in Array-Testformaten |
US20080138842A1 (en) * | 2006-12-11 | 2008-06-12 | Hans Boehringer | Indirect lateral flow sandwich assay |
CN102520191B (zh) * | 2011-12-19 | 2014-02-26 | 苏州大学 | 一种运用elisa测定蛋白质浓度的方法 |
CN106324253A (zh) * | 2016-08-12 | 2017-01-11 | 江苏泽成生物技术有限公司 | 一种梅毒螺旋体抗体测定试剂盒及其检测方法 |
US20220229062A1 (en) * | 2021-01-21 | 2022-07-21 | Diagnostic Biosystems | Method for rapid immunohistochemical detection of an antigen from a biological sample |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4228237A (en) * | 1978-09-21 | 1980-10-14 | Calbiochem-Behring Corp. | Methods for the detection and determination of ligands |
US4495296A (en) * | 1979-05-21 | 1985-01-22 | New York Blood Center, Inc. | Labeled anti-hapten antibodies and their use as a universal reagent for solid phase radio- and/or enzyme-immunoassays |
US4376110A (en) * | 1980-08-04 | 1983-03-08 | Hybritech, Incorporated | Immunometric assays using monoclonal antibodies |
US5011771A (en) * | 1984-04-12 | 1991-04-30 | The General Hospital Corporation | Multiepitopic immunometric assay |
US4743542A (en) * | 1985-04-11 | 1988-05-10 | Ortho Diagnostic | Method for forestalling the hook effect in a multi-ligand immunoassay system |
DE3834766A1 (de) * | 1988-10-12 | 1990-04-26 | Boehringer Mannheim Gmbh | Verfahren zur bestimmung einer spezifisch bindefaehigen substanz |
CA2008100A1 (en) * | 1989-01-20 | 1990-07-20 | Hubert Bayer | Method for the determination of immunologically detectable substance |
WO1991006857A1 (fr) * | 1989-11-02 | 1991-05-16 | Teijin Limited | Procede d'analyse immunologique de la thrombomoduline humaine, et reactif et kit utilises a cet effet |
-
1992
- 1992-06-03 DE DE4218257A patent/DE4218257A1/de not_active Withdrawn
-
1993
- 1993-05-14 EP EP93107931A patent/EP0572845B1/de not_active Expired - Lifetime
- 1993-05-14 AT AT93107931T patent/ATE164682T1/de not_active IP Right Cessation
- 1993-05-14 ES ES93107931T patent/ES2117067T3/es not_active Expired - Lifetime
- 1993-05-14 DE DE59308328T patent/DE59308328D1/de not_active Expired - Lifetime
- 1993-06-02 AU AU39966/93A patent/AU668937B2/en not_active Ceased
- 1993-06-02 CA CA002097545A patent/CA2097545C/en not_active Expired - Fee Related
- 1993-06-02 JP JP13156793A patent/JP3327488B2/ja not_active Expired - Fee Related
-
1995
- 1995-05-15 US US08/441,175 patent/US5914243A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
CA2097545A1 (en) | 1993-12-04 |
EP0572845B1 (de) | 1998-04-01 |
CA2097545C (en) | 2004-08-17 |
ATE164682T1 (de) | 1998-04-15 |
EP0572845A1 (de) | 1993-12-08 |
AU668937B2 (en) | 1996-05-23 |
AU3996693A (en) | 1993-12-09 |
JPH0658937A (ja) | 1994-03-04 |
US5914243A (en) | 1999-06-22 |
DE4218257A1 (de) | 1993-12-09 |
DE59308328D1 (de) | 1998-05-07 |
JP3327488B2 (ja) | 2002-09-24 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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