ES2112211A1 - Procedimiento para el estudio del ciclo celular de subpoblaciones de celulas presentes en muestras celulares heterogeneas. - Google Patents

Procedimiento para el estudio del ciclo celular de subpoblaciones de celulas presentes en muestras celulares heterogeneas.

Info

Publication number
ES2112211A1
ES2112211A1 ES09600715A ES9600715A ES2112211A1 ES 2112211 A1 ES2112211 A1 ES 2112211A1 ES 09600715 A ES09600715 A ES 09600715A ES 9600715 A ES9600715 A ES 9600715A ES 2112211 A1 ES2112211 A1 ES 2112211A1
Authority
ES
Spain
Prior art keywords
cell
analysis
procedure
cell cycle
heterogeneous
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES09600715A
Other languages
English (en)
Other versions
ES2112211B1 (es
Inventor
De Matos Correia E Vale Orfao
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universidad de Salamanca
Original Assignee
Universidad de Salamanca
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universidad de Salamanca filed Critical Universidad de Salamanca
Priority to ES09600715A priority Critical patent/ES2112211B1/es
Priority to EP97500058A priority patent/EP0798386B1/en
Priority to AT97500058T priority patent/ATE266100T1/de
Priority to DE69728925T priority patent/DE69728925T2/de
Publication of ES2112211A1 publication Critical patent/ES2112211A1/es
Application granted granted Critical
Publication of ES2112211B1 publication Critical patent/ES2112211B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Zoology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

PROCEDIMIENTO PARA EL ESTUDIO DEL CICLO CELULAR DE SUBPOBLACIONES DE CELULAS PRESENTES EN MUESTRAS CELULARES HETEROGENEAS. COMPRENDE: INCUBAR LA MUESTRA DE FORMA SECUENCIAL CON: 1) UN POOL DE ANTICUERPOS MONOCLONALES DIFERENTES MARCADOS CON UN MISMO FLUOROCROMO Y 2) CON UN FLUOROCROMO QUE SE UNE DE FORMA ESPECIFICA A ACIDOS NUCLEICOS; MEDIR POR CITOMETRIA DE FLUJO LAS EMISIONES FLUORESCENTES; DETERMINAR EL CICLO CELULAR DE FORMA ESPECIFICA DE LAS SUBPOBLACIONES DE CELULAS DE LA MUESTRA IDENTIFICADAS MEDIANTE ANALISIS MULTIPARAMETRICO QUE PERMITE CONOCER TODOS LOS TIPOS CELULARES IDENTIFICADOS LA PROPORCION DE CELULAS EN LAS FASES G0/G1, DE SINTESIS DE ADN (S) Y G2/MITOSIS (G2/M) DEL CICLO CELULAR.
ES09600715A 1996-03-26 1996-03-26 Procedimiento para el estudio del ciclo celular de subpoblaciones de celulas presentes en muestras celulares heterogeneas. Expired - Fee Related ES2112211B1 (es)

Priority Applications (4)

Application Number Priority Date Filing Date Title
ES09600715A ES2112211B1 (es) 1996-03-26 1996-03-26 Procedimiento para el estudio del ciclo celular de subpoblaciones de celulas presentes en muestras celulares heterogeneas.
EP97500058A EP0798386B1 (en) 1996-03-26 1997-03-25 Procedure for the analysis of the cell cycle of cell subpopulations present in heterogeneous cell samples
AT97500058T ATE266100T1 (de) 1996-03-26 1997-03-25 Verfahren zur analyse vom zellzyklus von zellsubpopulationen aus heterogenen zellproben
DE69728925T DE69728925T2 (de) 1996-03-26 1997-03-25 Verfahren zur Analyse vom Zellzyklus von Zellsubpopulationen aus heterogenen Zellproben

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES09600715A ES2112211B1 (es) 1996-03-26 1996-03-26 Procedimiento para el estudio del ciclo celular de subpoblaciones de celulas presentes en muestras celulares heterogeneas.

Publications (2)

Publication Number Publication Date
ES2112211A1 true ES2112211A1 (es) 1998-03-16
ES2112211B1 ES2112211B1 (es) 1998-12-16

Family

ID=8294309

Family Applications (1)

Application Number Title Priority Date Filing Date
ES09600715A Expired - Fee Related ES2112211B1 (es) 1996-03-26 1996-03-26 Procedimiento para el estudio del ciclo celular de subpoblaciones de celulas presentes en muestras celulares heterogeneas.

Country Status (4)

Country Link
EP (1) EP0798386B1 (es)
AT (1) ATE266100T1 (es)
DE (1) DE69728925T2 (es)
ES (1) ES2112211B1 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19935047C2 (de) * 1999-07-26 2001-05-31 Johannes L J Frank Multidimensionale Dynamische Cytofluorophorese (MDCFP)
US6884624B1 (en) * 2000-04-20 2005-04-26 Sandia Corporation Method for measuring the rate of cell reproduction by analysis of nanoliter cell samples
AU2005263993B2 (en) 2004-07-23 2009-11-12 Ge Healthcare Uk Limited Cell cycle reporting cell line
US7745123B2 (en) 2004-07-23 2010-06-29 Ge Healthcare Uk Limited Cell cycle reporting cell line
US8101368B2 (en) 2006-02-13 2012-01-24 Dvs Sciences Inc. Quantitation of cellular DNA and cell numbers using element labeling
RS59769B1 (sr) 2010-06-09 2020-02-28 Genmab As Antitela protiv humanog cd38
WO2013012752A2 (en) 2011-07-15 2013-01-24 Sarepta Therapeutics, Inc. Methods and compositions for manipulating translation of protein isoforms from alternative initiation start sites

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0121262A2 (en) * 1983-04-05 1984-10-10 Becton Dickinson and Company Method and apparatus for distinguishing multiple subpopulations of cells in a sample
US4780406A (en) * 1983-10-18 1988-10-25 The Regents Of The University Of California Flow cytometric measurement of total DNA and incorporated halodeoxyuridine
ES2051651B1 (es) * 1992-12-10 1995-01-01 Univ Salamanca Procedimiento para la cuantificacion simultanea, en una sola medicion, de los principales tipos de linfocitos humanos y sus subpoblaciones.

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0121262A2 (en) * 1983-04-05 1984-10-10 Becton Dickinson and Company Method and apparatus for distinguishing multiple subpopulations of cells in a sample
US4780406A (en) * 1983-10-18 1988-10-25 The Regents Of The University Of California Flow cytometric measurement of total DNA and incorporated halodeoxyuridine
ES2051651B1 (es) * 1992-12-10 1995-01-01 Univ Salamanca Procedimiento para la cuantificacion simultanea, en una sola medicion, de los principales tipos de linfocitos humanos y sus subpoblaciones.

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ANDREEFF M. et al. "Cellular ras Oncogene Expression and Cell Cycle Measured by Flow Cytometry in Hematopoietic Cell Lines". BLOOD. 1986. Vol. 67 (3), pßginas 676-681 *
McMURRAY B.P. et al. "Sequential flow cytometric analysis of cell-cycle related changes in LFA-1 (CD18 CD11A) expression by Trisomy 21 (Down's Syndrome) limphoblastoid cells" CELL TISSUE KINET. 1989. Vol. 22 (3), pßginas 223-233 *

Also Published As

Publication number Publication date
DE69728925D1 (de) 2004-06-09
DE69728925T2 (de) 2005-04-07
EP0798386A1 (en) 1997-10-01
EP0798386B1 (en) 2004-05-06
ATE266100T1 (de) 2004-05-15
ES2112211B1 (es) 1998-12-16

Similar Documents

Publication Publication Date Title
AU574281B2 (en) Process for the quantification of cell populations or subpopulations and a reagent suitable therefor
DK1064303T3 (da) Monoklonale antistoffer, der er specifikke for det prostataspecifikke membranantigens ekstracellulære domæne
IL126314A0 (en) Monoclonal specific antibodies
DE69622696D1 (de) Zellzählung durch einen immunoassay
ES2153134T3 (es) Dosificado inmunologico de topiramato, analogos y anticuerpos.
ES2051651A1 (es) Procedimiento para la cuantificacion simultanea, en una sola medicion, de los principales tipos de linfocitos humanos y sus subpoblaciones.
ES2112211A1 (es) Procedimiento para el estudio del ciclo celular de subpoblaciones de celulas presentes en muestras celulares heterogeneas.
KR890702039A (ko) 1,25-디하이드록시 비타민 d의 분석 방법
DK0460190T3 (da) Fremgangsmåde til påvisning af maligne sygdomme
RU2000114841A (ru) Антитела против фосфорилированного vasp (стимулированного вазодилататором фосфопротеина), гибридомные клетки для их получения и их применение
GR920100123A (el) Αντιδραστήριο πολυχρωματικής κηλίδωσης και μέ?οδος ανάλυσης.
Barnett et al. Automated immunofluorescent speciation of oral bacteria using flow cytometry
MY103022A (en) Monoclonal antibodies recognizing l-thyroxine
WO1998029726A3 (en) Method of detecting and determining the concentration of total troponin i in a biological sample
Rosato et al. Simultaneous analysis of surface marker expression and cell cycle progression in human peripheral blood mononuclear cells
Swirsky et al. The characterisation of monoclonal antibodies against haemopoietic cells: comparison of an immunoperoxidase method with fluorescence activated cell sorting
TR200100643T2 (tr) Yüksek-çekimli antikorlar
DK1192443T3 (da) Fremgangsmåde til kvantificering af endotel-mikrosfærer
US9285367B2 (en) Three-color reagent for measurement of CD4 positive lymphocytes by flow cytometry
Hiramatsu et al. Surface properties of LDL-binding lymphocytes in human peripheral blood.
Kleine et al. Segregation of reactivity to the mitogens lipopolysaccharide, lipoprotein and dextran sulfate in clones of the B cell lymphoma line WEHI 279.1
FR2599513B1 (fr) Anticorps monoclonaux inhibant la liaison de ige soluble aux lymphocytes humains, hybridomes monoclonaux produisant de tels anticorps, leur utilisation
ES8705232A1 (es) Un metodo para preparar un anticuerpo monoclonal destinado autilizarse como auxiliar de diagnostico para detectar cancerde pulmon
Vilpo et al. An improved radioimmunoassay for the quantitation of DNA methylation
Vitale et al. Improved bromodeoxyuridine/DNA analysis by anti-BudR monoclonal antibody versus right angle light scatter

Legal Events

Date Code Title Description
EC2A Search report published

Date of ref document: 19980316

Kind code of ref document: A1

Effective date: 19980316

FD2A Announcement of lapse in spain

Effective date: 20160701