ES2112211B1 - Procedimiento para el estudio del ciclo celular de subpoblaciones de celulas presentes en muestras celulares heterogeneas. - Google Patents
Procedimiento para el estudio del ciclo celular de subpoblaciones de celulas presentes en muestras celulares heterogeneas.Info
- Publication number
- ES2112211B1 ES2112211B1 ES09600715A ES9600715A ES2112211B1 ES 2112211 B1 ES2112211 B1 ES 2112211B1 ES 09600715 A ES09600715 A ES 09600715A ES 9600715 A ES9600715 A ES 9600715A ES 2112211 B1 ES2112211 B1 ES 2112211B1
- Authority
- ES
- Spain
- Prior art keywords
- cell
- cell cycle
- procedure
- cells present
- populations
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Zoology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
PROCEDIMIENTO PARA EL ESTUDIO DEL CICLO CELULAR DE SUBPOBLACIONES DE CELULAS PRESENTES EN MUESTRAS CELULARES HETEROGENEAS. COMPRENDE: INCUBAR LA MUESTRA DE FORMA SECUENCIAL CON: 1) UN POOL DE ANTICUERPOS MONOCLONALES DIFERENTES MARCADOS CON UN MISMO FLUOROCROMO Y 2) CON UN FLUOROCROMO QUE SE UNE DE FORMA ESPECIFICA A ACIDOS NUCLEICOS; MEDIR POR CITOMETRIA DE FLUJO LAS EMISIONES FLUORESCENTES; DETERMINAR EL CICLO CELULAR DE FORMA ESPECIFICA DE LAS SUBPOBLACIONES DE CELULAS DE LA MUESTRA IDENTIFICADAS MEDIANTE ANALISIS MULTIPARAMETRICO QUE PERMITE CONOCER TODOS LOS TIPOS CELULARES IDENTIFICADOS LA PROPORCION DE CELULAS EN LAS FASES G0/G1, DE SINTESIS DE ADN (S) Y G2/MITOSIS (G2/M) DEL CICLO CELULAR.
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES09600715A ES2112211B1 (es) | 1996-03-26 | 1996-03-26 | Procedimiento para el estudio del ciclo celular de subpoblaciones de celulas presentes en muestras celulares heterogeneas. |
AT97500058T ATE266100T1 (de) | 1996-03-26 | 1997-03-25 | Verfahren zur analyse vom zellzyklus von zellsubpopulationen aus heterogenen zellproben |
DE69728925T DE69728925T2 (de) | 1996-03-26 | 1997-03-25 | Verfahren zur Analyse vom Zellzyklus von Zellsubpopulationen aus heterogenen Zellproben |
EP97500058A EP0798386B1 (en) | 1996-03-26 | 1997-03-25 | Procedure for the analysis of the cell cycle of cell subpopulations present in heterogeneous cell samples |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES09600715A ES2112211B1 (es) | 1996-03-26 | 1996-03-26 | Procedimiento para el estudio del ciclo celular de subpoblaciones de celulas presentes en muestras celulares heterogeneas. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2112211A1 ES2112211A1 (es) | 1998-03-16 |
ES2112211B1 true ES2112211B1 (es) | 1998-12-16 |
Family
ID=8294309
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES09600715A Expired - Fee Related ES2112211B1 (es) | 1996-03-26 | 1996-03-26 | Procedimiento para el estudio del ciclo celular de subpoblaciones de celulas presentes en muestras celulares heterogeneas. |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP0798386B1 (es) |
AT (1) | ATE266100T1 (es) |
DE (1) | DE69728925T2 (es) |
ES (1) | ES2112211B1 (es) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE19935047C2 (de) * | 1999-07-26 | 2001-05-31 | Johannes L J Frank | Multidimensionale Dynamische Cytofluorophorese (MDCFP) |
US6884624B1 (en) * | 2000-04-20 | 2005-04-26 | Sandia Corporation | Method for measuring the rate of cell reproduction by analysis of nanoliter cell samples |
US7745123B2 (en) | 2004-07-23 | 2010-06-29 | Ge Healthcare Uk Limited | Cell cycle reporting cell line |
EP1771466B1 (en) | 2004-07-23 | 2010-05-26 | GE Healthcare UK Limited | Cell cycle phase markers |
US8101368B2 (en) | 2006-02-13 | 2012-01-24 | Dvs Sciences Inc. | Quantitation of cellular DNA and cell numbers using element labeling |
JP6093696B2 (ja) | 2010-06-09 | 2017-03-08 | ゲンマブ エー/エス | ヒトcd38に対する抗体 |
WO2013012752A2 (en) | 2011-07-15 | 2013-01-24 | Sarepta Therapeutics, Inc. | Methods and compositions for manipulating translation of protein isoforms from alternative initiation start sites |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS59184862A (ja) * | 1983-04-05 | 1984-10-20 | ベクトン・ディッキンソン・アンド・カンパニ− | 試料内細胞の複数副次集団を識別する方法と装置 |
US4780406A (en) * | 1983-10-18 | 1988-10-25 | The Regents Of The University Of California | Flow cytometric measurement of total DNA and incorporated halodeoxyuridine |
ES2051651B1 (es) * | 1992-12-10 | 1995-01-01 | Univ Salamanca | Procedimiento para la cuantificacion simultanea, en una sola medicion, de los principales tipos de linfocitos humanos y sus subpoblaciones. |
-
1996
- 1996-03-26 ES ES09600715A patent/ES2112211B1/es not_active Expired - Fee Related
-
1997
- 1997-03-25 AT AT97500058T patent/ATE266100T1/de not_active IP Right Cessation
- 1997-03-25 EP EP97500058A patent/EP0798386B1/en not_active Expired - Lifetime
- 1997-03-25 DE DE69728925T patent/DE69728925T2/de not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
DE69728925T2 (de) | 2005-04-07 |
ES2112211A1 (es) | 1998-03-16 |
EP0798386A1 (en) | 1997-10-01 |
EP0798386B1 (en) | 2004-05-06 |
DE69728925D1 (de) | 2004-06-09 |
ATE266100T1 (de) | 2004-05-15 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EC2A | Search report published |
Date of ref document: 19980316 Kind code of ref document: A1 Effective date: 19980316 |
|
FD2A | Announcement of lapse in spain |
Effective date: 20160701 |