ES2110462T3 - Modificacion del metabolismo de las celulas via nucleasas extracelulares. - Google Patents

Modificacion del metabolismo de las celulas via nucleasas extracelulares.

Info

Publication number
ES2110462T3
ES2110462T3 ES92119359T ES92119359T ES2110462T3 ES 2110462 T3 ES2110462 T3 ES 2110462T3 ES 92119359 T ES92119359 T ES 92119359T ES 92119359 T ES92119359 T ES 92119359T ES 2110462 T3 ES2110462 T3 ES 2110462T3
Authority
ES
Spain
Prior art keywords
cells
metabolism
modification
cells via
extra
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92119359T
Other languages
English (en)
Inventor
Scott E Tackett
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Application granted granted Critical
Publication of ES2110462T3 publication Critical patent/ES2110462T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/08—Reducing the nucleic acid content
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/10—Protozoa; Culture media therefor

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

LA INVENCION SE REFIERE A UN METODO PARA ALTERAR Y REGULAR LA EXPRESION DE GENES DE LAS CELULAS, POR MEDIO DE LA PUESTA EN CONTACTO DE LAS CELULAS CON UNA NUCLEASA CAPAZ DE DEGRAVAR EL DNA Y/O EL RNA EXTRACELULAR. LA NUCLEASA DEGRADARA LOS ACIDOS NUCLEICOS EXTRACELULARES (NA) EN NUCLEOTIDOS O OLIGONUCLEOTIDOS QUE SON DEMASIADO CORTOS PARA TENER AFINIDAD SUBSTANCIAL PARA EL DNA CROMOSOMICO. POR MEDIO DE ESTE METODO, SE HAN CREADO CULTIVOS DE CELULAS CON PROPIEDADES DESEABLES, INCLUYENDO MAYOR UNIFORMIDAD FENOTIPICA Y MAYORES NIVELES DE REPRODUCCION. LA INVENCION TAMBIEN COMPRENDE UN CULTIVO DE CELULAS QUE HA SIDO TRATADO MEDIANTE ESTE METODO, Y LAS CELULAS DESCENDIENTES DE CELULAS QUE HAN SIDO TRATADAS DE ACUERDO CON ESTE METODO.
ES92119359T 1987-03-23 1992-11-12 Modificacion del metabolismo de las celulas via nucleasas extracelulares. Expired - Lifetime ES2110462T3 (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US2927487A 1987-03-23 1987-03-23
EP92119359A EP0597146B1 (en) 1987-03-23 1992-11-12 Modifying cell metabolism via extra-cellular nucleases
BR9204509A BR9204509A (pt) 1987-03-23 1992-11-23 Processo para tratar células eucarioticas, processo para alterar a expressão de genes em células eucarióticas, processos para alterar a distribuição ou concentração e distribuição de ácido nucléico não cromossomômico em células eucarióticas, processo para alterar geneticamente células eucarióticas em um modo hereditário e cultura de células eucarióticas.
JP4351317A JPH06178690A (ja) 1987-03-23 1992-12-07 細胞外ヌクレアーゼによる細胞代謝の改変

Publications (1)

Publication Number Publication Date
ES2110462T3 true ES2110462T3 (es) 1998-02-16

Family

ID=27425214

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92119359T Expired - Lifetime ES2110462T3 (es) 1987-03-23 1992-11-12 Modificacion del metabolismo de las celulas via nucleasas extracelulares.

Country Status (6)

Country Link
EP (1) EP0597146B1 (es)
JP (1) JPH06178690A (es)
AT (1) ATE159048T1 (es)
BR (1) BR9204509A (es)
DE (1) DE69222645T2 (es)
ES (1) ES2110462T3 (es)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5006472A (en) * 1988-06-03 1991-04-09 Miles Inc. Enzymatic purification process
DE3939771A1 (de) * 1989-12-01 1991-06-06 Behringwerke Ag Verfahren zur biologischen inaktivierung von nukleinsaeuren

Also Published As

Publication number Publication date
ATE159048T1 (de) 1997-10-15
DE69222645D1 (de) 1997-11-13
JPH06178690A (ja) 1994-06-28
DE69222645T2 (de) 1998-02-05
EP0597146B1 (en) 1997-10-08
BR9204509A (pt) 1994-05-31
EP0597146A1 (en) 1994-05-18

Similar Documents

Publication Publication Date Title
DE69943389D1 (de) Verfahren und mittel zum erhalt von veränderten phänotypen
BR8200191A (pt) Celula viva renina preprorrenina prorrenina processo para obter expressao de preporrenina prorrenina e renina gene de prorrenina preprorrenina e renina e coli material dna recombinador saccharomyces cerevisiae polipetidio processo para exprimir um gene
HUP0202705A2 (hu) Eljárások és mikroorganizmusok pantovegyületek előállítására
DE69627698T2 (de) Eine in kaskaden verlaufende vervielfältigungsreaktion von nukleinsäuren
LTIP1826A (en) Process for preparing transformed prokaryotic host cell, stable gene amplification in the chromosome dna of prokaryotic microorganisms
WO1998012311A3 (en) Use of a non-mammalian dna virus to express an exogenous gene in a mammalian cell
DK1210123T3 (da) Enkeltstrengede oligodeoxynucleotidvektorer egnede til mutation
ES2180579T3 (es) Transferencia de genes en celulas epiteliales pancreaticas.
EP0928290A4 (en) OLIGORIBONUCLEOTIDES AND RIBONUCLEASES FOR THE CLIPPING OF RNS
ES2056799T3 (es) Moleculas de adn codante para las regiones de control fmdh y gen estructural para una proteina que tiene una actividad de fmdh y su uso.
ATE414767T1 (de) Nukleinsäure-linker und deren verwendung in der gensynthese
EP0792375A4 (en) ENZYMATIC DNA MOLECULES
ATE541940T1 (de) Intakte minizellen als vektoren für dna-transfer und gentherapie in vitro und in vivo
JPS55156591A (en) Method for stabilizing property of bacteria containing plasmid
Usher et al. Optimizing CRISPR/Cas9 editing of repetitive single nucleotide variants
Voß et al. Production of supercoiled multimeric plasmid DNA for biopharmaceutical application
AR043862A1 (es) Procedimiento para la obtencion de estirpes celulares de mastocitos a partir de tejidos de cerdo y procedimiento para la produccion de moleculas de tipo de la heparina
Tarashima et al. Gene Expression of 4′-Thioguanine DNA via 4′-Thiocytosine RNA
ES2110462T3 (es) Modificacion del metabolismo de las celulas via nucleasas extracelulares.
HUP0101961A2 (hu) Az FSH-béta-gén módosítása homológ rekombinációval
FI793884A (fi) Stabila plasmider med stort kopieantal
Takayuki et al. Decomposition of nucleic acid molecules in pulsed electric field and its release from recombinantEscherichia coli
DE69111371D1 (de) Synthetisches Gen für menschliches Parathyroidhormon.
ATE113655T1 (de) Expressionsvektor zur regulierbaren expression von fremdgenen in prokaryonten.
SE9402642D0 (sv) Gene Therapy

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 597146

Country of ref document: ES