ES2106737T3 - Enzimas de fosfatasa alcalina con actividad especifica mejorada para uso en reactivos indicadores. - Google Patents

Enzimas de fosfatasa alcalina con actividad especifica mejorada para uso en reactivos indicadores.

Info

Publication number
ES2106737T3
ES2106737T3 ES91101338T ES91101338T ES2106737T3 ES 2106737 T3 ES2106737 T3 ES 2106737T3 ES 91101338 T ES91101338 T ES 91101338T ES 91101338 T ES91101338 T ES 91101338T ES 2106737 T3 ES2106737 T3 ES 2106737T3
Authority
ES
Spain
Prior art keywords
alkaline phosphatase
specific activity
image
improved specific
phosphatase enzymes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91101338T
Other languages
English (en)
Inventor
Wlodzimierz Mandecki
Susan J Tomazic-Allen
Mary Ann Shallcross
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Application granted granted Critical
Publication of ES2106737T3 publication Critical patent/ES2106737T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates

Abstract

LA PRESENTE INVENCION, INCLUYE LA REALIZACION DE ALGUNAS MUTACIONES EN EL GEN ALCALINO FOSFATOSO DE LA ESCHERICHIA COLI, QUE TIENE COMO RESULTADO LA PRODUCCION DE UNA ENCIMA CON UNA ACTIVIDAD ESPECIFICA INCREMENTADA. LAS ENCIMAS MUTANTES OBTENIDAS, TIENEN DE 1,5 HASTA 36 DOBLE ACTIVIDAD ESPECIFICA SUPERIOR, MIENTRAS QUE MANTIENEN LA ESTABILIDAD CON LA TEMPERATURA, SIGNIFICATIVAMENTE MAYOR QUE LAS ENCIMAS ALCALINO FOSFATOSAS DE LOS MAMIFEROS. LA UTILIDAD DE LA ALCALINO FOSFATOSA MUTANTE DE LA E. COLI, COMO ETIQUETA ENCIMATICA EN LOS ENSAYOS OBLIGATORIOS, ESTA TAMBIEN DEMOSTRADA.
ES91101338T 1990-02-07 1991-02-01 Enzimas de fosfatasa alcalina con actividad especifica mejorada para uso en reactivos indicadores. Expired - Lifetime ES2106737T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US47619990A 1990-02-07 1990-02-07

Publications (1)

Publication Number Publication Date
ES2106737T3 true ES2106737T3 (es) 1997-11-16

Family

ID=23890914

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91101338T Expired - Lifetime ES2106737T3 (es) 1990-02-07 1991-02-01 Enzimas de fosfatasa alcalina con actividad especifica mejorada para uso en reactivos indicadores.

Country Status (8)

Country Link
EP (1) EP0441252B1 (es)
JP (1) JP2620416B2 (es)
KR (1) KR910015700A (es)
AT (1) ATE157702T1 (es)
AU (1) AU656313B2 (es)
CA (1) CA2035826A1 (es)
DE (1) DE69127482T2 (es)
ES (1) ES2106737T3 (es)

Families Citing this family (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994001531A1 (en) * 1992-07-07 1994-01-20 Abbott Laboratories Alkaline phosphatase enzymes having improved specific activity for use in indicator reagents
US5304472A (en) * 1992-11-20 1994-04-19 Genentech, Inc. Method of controlling polypeptide production in bacterial cells
JPH08507686A (ja) * 1993-03-09 1996-08-20 アボツト・ラボラトリーズ 遺伝子操作酵素及びそれらの診断アッセイ用結合体
WO1995005455A1 (en) * 1993-08-13 1995-02-23 Rijksuniversiteit Te Groningen Pharmaceutical composition comprising phosphatase or a derivative thereof
DE69631787T2 (de) * 1995-12-07 2005-03-10 Diversa Corp., San Diego Verfahren zum screening enzymatischer aktivität
US7885697B2 (en) 2004-07-13 2011-02-08 Dexcom, Inc. Transcutaneous analyte sensor
US7432097B2 (en) 1997-08-13 2008-10-07 Verenium Corporation Phytases, nucleic acids encoding them and methods of making and using them
US7078035B2 (en) 1997-08-13 2006-07-18 Diversa Corporation Phytases, nucleic acids encoding them and methods for making and using them
US6720014B1 (en) 1997-08-13 2004-04-13 Diversa Corporation Phytase-containing foodstuffs and methods of making and using them
US6183740B1 (en) 1997-08-13 2001-02-06 Diversa Corporation Recombinant bacterial phytases and uses thereof
US8260393B2 (en) 2003-07-25 2012-09-04 Dexcom, Inc. Systems and methods for replacing signal data artifacts in a glucose sensor data stream
US9282925B2 (en) 2002-02-12 2016-03-15 Dexcom, Inc. Systems and methods for replacing signal artifacts in a glucose sensor data stream
US8010174B2 (en) 2003-08-22 2011-08-30 Dexcom, Inc. Systems and methods for replacing signal artifacts in a glucose sensor data stream
WO2004076628A2 (en) 2003-02-24 2004-09-10 New England Biolabs, Inc. Overexpression, purification and characterization of a thermolabile phosphatase
US8423113B2 (en) 2003-07-25 2013-04-16 Dexcom, Inc. Systems and methods for processing sensor data
US7774145B2 (en) 2003-08-01 2010-08-10 Dexcom, Inc. Transcutaneous analyte sensor
US6931327B2 (en) 2003-08-01 2005-08-16 Dexcom, Inc. System and methods for processing analyte sensor data
US8369919B2 (en) 2003-08-01 2013-02-05 Dexcom, Inc. Systems and methods for processing sensor data
US8275437B2 (en) 2003-08-01 2012-09-25 Dexcom, Inc. Transcutaneous analyte sensor
US8761856B2 (en) 2003-08-01 2014-06-24 Dexcom, Inc. System and methods for processing analyte sensor data
US20190357827A1 (en) 2003-08-01 2019-11-28 Dexcom, Inc. Analyte sensor
US20100168657A1 (en) 2003-08-01 2010-07-01 Dexcom, Inc. System and methods for processing analyte sensor data
US7591801B2 (en) 2004-02-26 2009-09-22 Dexcom, Inc. Integrated delivery device for continuous glucose sensor
US8160669B2 (en) 2003-08-01 2012-04-17 Dexcom, Inc. Transcutaneous analyte sensor
US7925321B2 (en) 2003-08-01 2011-04-12 Dexcom, Inc. System and methods for processing analyte sensor data
US7920906B2 (en) 2005-03-10 2011-04-05 Dexcom, Inc. System and methods for processing analyte sensor data for sensor calibration
US20140121989A1 (en) 2003-08-22 2014-05-01 Dexcom, Inc. Systems and methods for processing analyte sensor data
US9247900B2 (en) 2004-07-13 2016-02-02 Dexcom, Inc. Analyte sensor
WO2005051170A2 (en) 2003-11-19 2005-06-09 Dexcom, Inc. Integrated receiver for continuous analyte sensor
US11633133B2 (en) 2003-12-05 2023-04-25 Dexcom, Inc. Dual electrode system for a continuous analyte sensor
US8423114B2 (en) 2006-10-04 2013-04-16 Dexcom, Inc. Dual electrode system for a continuous analyte sensor
US8364231B2 (en) 2006-10-04 2013-01-29 Dexcom, Inc. Analyte sensor
US8774886B2 (en) 2006-10-04 2014-07-08 Dexcom, Inc. Analyte sensor
EP2239566B1 (en) 2003-12-05 2014-04-23 DexCom, Inc. Calibration techniques for a continuous analyte sensor
WO2005057175A2 (en) 2003-12-09 2005-06-23 Dexcom, Inc. Signal processing for continuous analyte sensor
US8808228B2 (en) 2004-02-26 2014-08-19 Dexcom, Inc. Integrated medicament delivery device for use with continuous analyte sensor
US8452368B2 (en) 2004-07-13 2013-05-28 Dexcom, Inc. Transcutaneous analyte sensor
US7857760B2 (en) 2004-07-13 2010-12-28 Dexcom, Inc. Analyte sensor
EP1991110B1 (en) 2006-03-09 2018-11-07 DexCom, Inc. Systems and methods for processing analyte sensor data
EP1985697A1 (en) 2007-04-27 2008-10-29 AM-Pharma B.V. Modified phosphatases
CA2688184A1 (en) 2007-06-08 2008-12-18 Dexcom, Inc. Integrated medicament delivery device for use with continuous analyte sensor
US9452258B2 (en) 2007-10-09 2016-09-27 Dexcom, Inc. Integrated insulin delivery system with continuous glucose sensor
CA2715628A1 (en) 2008-02-21 2009-08-27 Dexcom, Inc. Systems and methods for processing, transmitting and displaying sensor data
US9801575B2 (en) 2011-04-15 2017-10-31 Dexcom, Inc. Advanced analyte sensor calibration and error detection
EP3925533B1 (en) 2009-04-30 2024-04-10 DexCom, Inc. Performance reports associated with continuous sensor data from multiple analysis time periods
NZ596459A (en) 2009-05-21 2013-11-29 Verenium Corp Phytases, nucleic acids encoding them and methods for making and using them
EP2620162A1 (de) 2012-01-27 2013-07-31 Life Science Inkubator Enzym-Polymer-Konjugat zur Endotoxin-Detoxifikation
ES2710663T3 (es) * 2012-02-16 2019-04-26 Elanco Us Inc Procedimientos y composiciones para reducir el impacto ambiental de residuos animales
JP5866715B2 (ja) 2013-10-18 2016-02-17 国立大学法人九州大学 タンパク質検出用融合タンパク質およびタンパク質の検出方法
DK3097188T3 (en) 2014-01-24 2018-10-08 Am Pharma Bv DOWNSTREAM PREPARATION OF AN ALKALIC PHOSPHATASE
CN106164262B (zh) 2014-01-24 2020-08-07 安-法玛公司 嵌合型碱性磷酸酶样蛋白
US11331022B2 (en) 2017-10-24 2022-05-17 Dexcom, Inc. Pre-connected analyte sensors
US20190120785A1 (en) 2017-10-24 2019-04-25 Dexcom, Inc. Pre-connected analyte sensors

Also Published As

Publication number Publication date
ATE157702T1 (de) 1997-09-15
DE69127482T2 (de) 1998-03-19
EP0441252A3 (en) 1991-11-21
AU656313B2 (en) 1995-02-02
JPH04349881A (ja) 1992-12-04
CA2035826A1 (en) 1991-08-08
EP0441252A2 (en) 1991-08-14
AU7083791A (en) 1991-08-08
DE69127482D1 (de) 1997-10-09
JP2620416B2 (ja) 1997-06-11
EP0441252B1 (en) 1997-09-03
KR910015700A (ko) 1991-09-30

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