ES2103902T3 - Promotor regulado por calcio. - Google Patents

Promotor regulado por calcio.

Info

Publication number
ES2103902T3
ES2103902T3 ES92311292T ES92311292T ES2103902T3 ES 2103902 T3 ES2103902 T3 ES 2103902T3 ES 92311292 T ES92311292 T ES 92311292T ES 92311292 T ES92311292 T ES 92311292T ES 2103902 T3 ES2103902 T3 ES 2103902T3
Authority
ES
Spain
Prior art keywords
polypeptide
enzyme
regulated promoter
recombinant vector
operatively linked
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92311292T
Other languages
English (en)
Inventor
Antonio Daza Ortega
Jose Antonio Gil
Tomas Vigal Garcia
Juan Francisco Martin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck SL
Original Assignee
Laboratorios Serono SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Laboratorios Serono SA filed Critical Laboratorios Serono SA
Application granted granted Critical
Publication of ES2103902T3 publication Critical patent/ES2103902T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/11—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of two atoms of oxygen (1.13.11)
    • C12Y113/11001—Catechol 1,2-dioxygenase (1.13.11.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/11—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of two atoms of oxygen (1.13.11)
    • C12Y113/11002—Catechol 2,3-dioxygenase (1.13.11.2)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fodder In General (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Dental Preparations (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Hydrogenated Pyridines (AREA)

Abstract

SE PRESENTA UN NUEVO PROMOTOR DE CALCIO REGULADO PARA UTILIZARLO PARA AUMENTAR LA PRODUCCION DE ENZIMAS EXTRACELULARES O POLIPEPTIDOS HETEROLOGOS, UN VECTOR QUE INCLUYE LA SECUENCIA DE ADN DEL PROMOTOR OPERATIVAMENTE ENLAZADA A UN ADN QUE CODIFICA DICHA ENZIMA O POLIPEPTIDO Y UN ORGANISMO HUESPED TRANSFORMADO CON EL VECTOR RECOMBINANTE QUE INCLUYE EL PROMOTOR OPERATIVAMENTE ENLAZADO AL ADN QUE CODIFICA DICHA ENZIMA O POLIPEPTIDO. LA PRESENTE INVENCION SE REFIERE ADEMAS A SISTEMAS DE EXPRESION DE STREPTOMYCES Y A METODOS PARA LA EXPRESION DE SECUENCIAS DE ADN EXTRAÑAS EN STREPTOMYCES Y PAR SEGREGAR EN EL MEDIO CIRCUNDANTE POLIPEPTIDOS Y PROTEINAS CODIFICADOS POR ESAS SECUENCIAS DE ADN EXTRAÑAS.
ES92311292T 1991-12-12 1992-12-10 Promotor regulado por calcio. Expired - Lifetime ES2103902T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9102765 1991-12-12

Publications (1)

Publication Number Publication Date
ES2103902T3 true ES2103902T3 (es) 1997-10-01

Family

ID=8274462

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92311292T Expired - Lifetime ES2103902T3 (es) 1991-12-12 1992-12-10 Promotor regulado por calcio.

Country Status (15)

Country Link
US (2) US5385841A (es)
EP (1) EP0546833B1 (es)
JP (1) JP3340164B2 (es)
AT (1) ATE154392T1 (es)
AU (1) AU661073B2 (es)
CA (1) CA2084351C (es)
DE (1) DE69220336T2 (es)
DK (1) DK0546833T3 (es)
ES (1) ES2103902T3 (es)
GR (1) GR3024625T3 (es)
IL (1) IL103975A (es)
LT (1) LT3133B (es)
LV (1) LV10728B (es)
RU (1) RU2112802C1 (es)
ZA (1) ZA929284B (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA929284B (en) * 1991-12-12 1993-06-02 Serono Lab P0438, an new calcium-regulated promoter.
US6381353B1 (en) 1996-08-30 2002-04-30 Marvin Weiss System for counting colonies of micro-organisms in petri dishes and other culture media

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8315579D0 (en) 1983-06-07 1983-07-13 Biogen Nv Streptomyces expression systems
US4766066A (en) * 1984-09-27 1988-08-23 Eli Lilly And Company Method of using bacteriophage lambda p1 promoter to produce a functional polypeptide in streptomyces
US4745056A (en) * 1984-10-23 1988-05-17 Biotechnica International, Inc. Streptomyces secretion vector
DE3536182A1 (de) * 1985-10-10 1987-04-16 Hoechst Ag Promotor-anordnung fuer streptomyceten-vektoren
US5200327A (en) * 1985-11-06 1993-04-06 Cangene Corporation Expression system for the secretion of bioactive human granulocyte macrophage colony stimulating factor (GM-CSF) and other heterologous proteins from streptomyces
AU1299988A (en) 1987-03-09 1988-10-10 Cetus Corporation Expression of heterologous genes in streptomyces species
US5118617A (en) * 1989-05-19 1992-06-02 Applied Research Systems Ars Holding N.V. Saf polypeptide, gene coding therefor, saf promoter and expression methods for expressing foreign dna sequences in streptomyces
ZA929284B (en) * 1991-12-12 1993-06-02 Serono Lab P0438, an new calcium-regulated promoter.

Also Published As

Publication number Publication date
LTIP237A (en) 1994-07-15
CA2084351A1 (en) 1993-06-13
DE69220336T2 (de) 1997-09-25
JP3340164B2 (ja) 2002-11-05
US5660999A (en) 1997-08-26
LT3133B (en) 1995-01-31
EP0546833A3 (es) 1994-04-06
EP0546833B1 (en) 1997-06-11
EP0546833A2 (en) 1993-06-16
AU2979192A (en) 1993-06-17
IL103975A0 (en) 1993-05-13
AU661073B2 (en) 1995-07-13
ATE154392T1 (de) 1997-06-15
LV10728A (lv) 1995-06-20
JPH05336972A (ja) 1993-12-21
CA2084351C (en) 2007-04-10
ZA929284B (en) 1993-06-02
DK0546833T3 (da) 1997-09-15
DE69220336D1 (de) 1997-07-17
LV10728B (en) 1995-12-20
GR3024625T3 (es) 1997-12-31
IL103975A (en) 1998-08-16
RU2112802C1 (ru) 1998-06-10
US5385841A (en) 1995-01-31

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