ES2103749T3 - Metodo para la obtencion de una proteina recombinante y su utilizacion en la deteccion del virus de la peste porcina africana (vppa). - Google Patents

Metodo para la obtencion de una proteina recombinante y su utilizacion en la deteccion del virus de la peste porcina africana (vppa).

Info

Publication number
ES2103749T3
ES2103749T3 ES90916994T ES90916994T ES2103749T3 ES 2103749 T3 ES2103749 T3 ES 2103749T3 ES 90916994 T ES90916994 T ES 90916994T ES 90916994 T ES90916994 T ES 90916994T ES 2103749 T3 ES2103749 T3 ES 2103749T3
Authority
ES
Spain
Prior art keywords
protein
obtaining
gene
recombinant protein
ppv
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90916994T
Other languages
English (en)
Inventor
Otin Carlos Lopez
Freije Jose Maria Perez
Diaz Eladio Vinuela
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
Universidad de Oviedo
Original Assignee
Consejo Superior de Investigaciones Cientificas CSIC
Universidad de Oviedo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Superior de Investigaciones Cientificas CSIC, Universidad de Oviedo filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Application granted granted Critical
Publication of ES2103749T3 publication Critical patent/ES2103749T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/12011—Asfarviridae
    • C12N2710/12022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

EL OBJETIVO DEL METODO ES LA DETECCION DEL VIRUS DE LA PESTE PORCINA AFRICANA MEDIANTE UNA PRUEBA INMUNO ENZIMATICA DONDE LA PRINCIPAL PROTEINA DEL VIRUS (P72) ES UTILIZADO COMO ANTIGENO, DICHA PROTEINA ES OBTENIDA POR MANIFESTACION DEL GENE QUE LA CODIFICA. EL GENE SE IDENTIFICA POR HIBRIDACION CON ENSAYOS OLIGONUCLEOTIDOS DERIVADOS DE LA INFORMACION ESTRUCTURAL DE LA PROTEINA PURIFICADA. UNA VEZ QUE LA SECUENCIA DE NUCLEOTIDOS HA SIDO DETERMINADA, EL GENE ES INSERTADO EN EL VECTOR DE FORMULACION PAR3038 AL OBJETO DE OBTENER EL ASOCIANTE DEL PLASMA PS72A. DICHO PLASMIDO HA SIDO UTILIZADO EN LA TRANSFORMACION DE LA CEPA E.COLI BL21(DE3) QUE, TRAS LA INDUCCION CON IPTG, FAVORECE EL ALTO RENDIMIENTO DE PRODUCCION DE PROTEINA ASOCIANTE P72. LA PROTEINA HA SIDO PURIFICADA POR UNA ELEVADA SOLUCION LIQUIDA CROMATOGRAFICA HABIENDOSE SIDO EMPLEADA DIRECTAMENTE EN ENSAYOS ANTIGENEÑANTICUERPO CON MUESTRAS DE SUERO ANIMAL A FIN DE DIAGNOSTICAR LA ENFERMEDAD.
ES90916994T 1989-12-20 1990-11-14 Metodo para la obtencion de una proteina recombinante y su utilizacion en la deteccion del virus de la peste porcina africana (vppa). Expired - Lifetime ES2103749T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES8904300A ES2024734A6 (es) 1989-12-20 1989-12-20 Procedimiento de obtencio de un metodo de ensayo para el virus de la peste porcina africana (vppa).

Publications (1)

Publication Number Publication Date
ES2103749T3 true ES2103749T3 (es) 1997-10-01

Family

ID=8265188

Family Applications (2)

Application Number Title Priority Date Filing Date
ES8904300A Expired - Fee Related ES2024734A6 (es) 1989-12-20 1989-12-20 Procedimiento de obtencio de un metodo de ensayo para el virus de la peste porcina africana (vppa).
ES90916994T Expired - Lifetime ES2103749T3 (es) 1989-12-20 1990-11-14 Metodo para la obtencion de una proteina recombinante y su utilizacion en la deteccion del virus de la peste porcina africana (vppa).

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES8904300A Expired - Fee Related ES2024734A6 (es) 1989-12-20 1989-12-20 Procedimiento de obtencio de un metodo de ensayo para el virus de la peste porcina africana (vppa).

Country Status (6)

Country Link
EP (1) EP0458918B1 (es)
AT (1) ATE152836T1 (es)
DE (1) DE69030655D1 (es)
ES (2) ES2024734A6 (es)
OA (1) OA09643A (es)
WO (1) WO1991009313A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100359022C (zh) * 2006-01-06 2008-01-02 云南出入境检验检疫局检验检疫技术中心 非洲猪瘟病毒的荧光定量pcr检测试剂及制备方法和应用
EP2441831B9 (en) * 2009-06-11 2019-03-06 Inter-University Research Institute Corporation Research Organization of Information and Systems Process for production of protein
CN110951920B (zh) * 2019-12-26 2021-01-15 武汉博杰生物医学科技有限公司 非洲猪瘟病毒的检测试剂盒与检测方法
CN113698474B (zh) * 2020-05-21 2023-06-02 河南省生物工程技术研究中心 一种非洲猪瘟多克隆抗体及非洲猪瘟抗原检测试纸条
CN113151187B (zh) * 2021-03-26 2023-04-18 南京农业大学 一株非洲猪瘟病毒单克隆抗体杂交瘤细胞及其应用

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4735800A (en) * 1983-09-09 1988-04-05 Molecular Genetics, Inc. Vaccines against rift valley fever virus

Also Published As

Publication number Publication date
DE69030655D1 (de) 1997-06-12
ES2024734A6 (es) 1992-03-01
ATE152836T1 (de) 1997-05-15
EP0458918A1 (en) 1991-12-04
OA09643A (en) 1993-04-30
WO1991009313A1 (es) 1991-06-27
EP0458918B1 (en) 1997-05-07

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