ES2098781T3 - Metodo y estuches de ensayo para la deteccion de bacterias. - Google Patents

Metodo y estuches de ensayo para la deteccion de bacterias.

Info

Publication number
ES2098781T3
ES2098781T3 ES93920986T ES93920986T ES2098781T3 ES 2098781 T3 ES2098781 T3 ES 2098781T3 ES 93920986 T ES93920986 T ES 93920986T ES 93920986 T ES93920986 T ES 93920986T ES 2098781 T3 ES2098781 T3 ES 2098781T3
Authority
ES
Spain
Prior art keywords
phage
detection
bacteria
specific
test cases
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93920986T
Other languages
English (en)
Inventor
Michael Frederick Sanders
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Minister of Agriculture Fisheries and Food UK
Original Assignee
Minister of Agriculture Fisheries and Food UK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Minister of Agriculture Fisheries and Food UK filed Critical Minister of Agriculture Fisheries and Food UK
Application granted granted Critical
Publication of ES2098781T3 publication Critical patent/ES2098781T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2304/00—Chemical means of detecting microorganisms
    • C12Q2304/60—Chemiluminescent detection using ATP-luciferin-luciferase system
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/24—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • G01N2333/255—Salmonella (G)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

SE DESCRIBE UN METODO PARA LA DETECCION, IDENTIFICACION Y/O CUANTIFICACION DE ORGANISMOS OBJETIVOS DE GENES BACTERIALES ESPECIFICOS, ESPECIES O SEROTIPOS, BASADO EN EL CASO DE LIBERACION DE CONTENIDOS CELULARES, PARTICULARMENTE NUCLEOTIDOS EJ. ATP SOBRE LA LISIS DE PAREDES CELULARES BACTERIANAS EN LA INCUBACION CON BACTERIOFAGOS ESPECIFICAS PARA ELLOS. CUANDO LAS PARTICULAS DE NUEVA FAGE SON LIBERADAS AL FINAL DEL CICLO DE REPLICACION DE FAGE, LOS NIVELES DE NUCLEOTIDOS SON MEDIDOS Y COMPARADOS CON CONTROLES. EL METODO PROPORCIONA LA DETECCION DE BACTERIAS ESPECIFICAS EN LAS QUE LA INSERCION DE LOS GENES LUX DEL GENOMA FAGE NO SE REQUIERE YA QUE ES MAS FACIL Y SENSIBLE QUE LAS TECNICAS QUE UTILIZAN FAGES NO MODIFICADAS CONOCIDAS. EL METODO ESTA SOLO LIMITADO POR LA DISPONIBILIDAD DE TIPOS DE FAGES ADECUADOS PARA EL ATAQUE SELECTIVO DE LA BACTERIA OBJETIVO QUE DEBE DETECTARSE Y PUEDE DETECTAR UNA SALMONELA SIMPLE EN UNA MUESTRA DE LECHE EN MENOS DE 12 HORAS.
ES93920986T 1992-09-22 1993-09-22 Metodo y estuches de ensayo para la deteccion de bacterias. Expired - Lifetime ES2098781T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB929220027A GB9220027D0 (en) 1992-09-22 1992-09-22 Method and kits for detection of bacteria

Publications (1)

Publication Number Publication Date
ES2098781T3 true ES2098781T3 (es) 1997-05-01

Family

ID=10722313

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93920986T Expired - Lifetime ES2098781T3 (es) 1992-09-22 1993-09-22 Metodo y estuches de ensayo para la deteccion de bacterias.

Country Status (13)

Country Link
EP (1) EP0663016B1 (es)
JP (1) JP3229884B2 (es)
AT (1) ATE149572T1 (es)
AU (1) AU677223B2 (es)
DE (1) DE69308581T2 (es)
DK (1) DK0663016T3 (es)
ES (1) ES2098781T3 (es)
GB (2) GB9220027D0 (es)
GR (1) GR3023654T3 (es)
IL (1) IL107027A (es)
NZ (1) NZ256076A (es)
WO (1) WO1994006931A1 (es)
ZA (1) ZA936813B (es)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2295893B (en) * 1993-08-18 1997-10-08 Mini Agriculture & Fisheries Method and test kits for detection of bacteriophage
GB9317139D0 (en) * 1993-08-18 1993-10-06 Miniser For Agriculture Fisher Method and test kits for detection of bacteriophage
EP0781349B1 (de) * 1994-09-09 2003-01-15 Biotec Laboratories Limited Aufschluss von bakterienzellen durch phagenlysine
BR9907161A (pt) * 1998-01-21 2000-10-24 Secr Defence Uso de um ensaio quanto à adenilato quinase em um teste in vitro, processos para determinar a suscetibilidade de uma bactéria a um reagente, para determinar a sensibilidade de uma bactéria a um antibiótico ou agente bioestático, para determinar a fase de cultivo de uma cultura bacteriana , e, kit de teste para analisar a sensibilidade de bactérias a antibióticos
NZ508724A (en) 1998-06-04 2003-04-29 Microsens Biophage Ltd Analytical method using at least two viruses to detect a target material in a sample
PT1198713E (pt) * 1999-07-30 2006-06-30 Profos Ag Deteccao e identificacao de estirpes bacterianas
CN1260353C (zh) 2001-07-18 2006-06-21 波兰科学院免疫和实验治疗研究所 用于治疗细菌感染的多价噬菌体制备方法
US7166425B2 (en) * 2002-04-12 2007-01-23 Colorado School Of Mines Method for detecting low concentrations of a target bacterium that uses phages to infect target bacterial cells
US8216780B2 (en) 2002-04-12 2012-07-10 Microphage (Tm) Incorporated Method for enhanced sensitivity in bacteriophage-based diagnostic assays
US8092990B2 (en) 2005-03-31 2012-01-10 Colorado School Of Mines Apparatus and method for detecting microscopic organisms using bacteriophage
GB0606822D0 (en) 2006-04-05 2006-05-17 Alaska Food Diagnostics Assay System
JP5150856B2 (ja) 2007-06-15 2013-02-27 マイクロファージ・インコーポレーテッド バクテリオファージ増幅を向上させた微生物の検出方法
US8697434B2 (en) 2008-01-11 2014-04-15 Colorado School Of Mines Detection of phage amplification by SERS nanoparticles
US9441204B2 (en) 2008-04-03 2016-09-13 Colorado School Of Mines Compositions and methods for detecting Yersinia pestis bacteria
PL215522B1 (pl) 2008-09-29 2013-12-31 Inst Immunologii I Terapii Doswiadczalnej Pan Nowe szczepy bakteriofagów do leczenia zakazen bakteryjnych, zwlaszcza szczepami bakterii lekoopornych rodzaju Enterococcus
PL212258B1 (pl) 2010-06-20 2012-09-28 Inst Immunologii I Terapii Doswiadczalnej Pan Nowe szczepy bakteriofagów do leczenia zakazen bakteryjnych, zwlaszcza szczepami bakterii lekoopornych rodzaju Stenotrophomonas
WO2012158041A1 (en) * 2011-05-18 2012-11-22 Rna Holding B.V. Diagnostic methods and kits for determining the presence of a microorganism in a sample
WO2013024421A1 (en) * 2011-08-12 2013-02-21 Universidade Do Minho Peptides and derivatives thereof for detection and control of salmonella
CN109750085A (zh) * 2019-03-18 2019-05-14 希拓生物科技有限公司 一种精准快速检测目标超级细菌的检测方法
CZ309429B6 (cs) * 2020-03-31 2023-01-04 MB PHARMA s.r.o Lyofilizovaná testovací sada ke stanovení antimikrobiální biologické účinnosti fágových přípravků na bakterii Staphylococcus aureus

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5558096A (en) * 1978-10-25 1980-04-30 Noda Sangyo Kagaku Kenkyusho Method of making novel recombined dna
GB8728296D0 (en) * 1987-12-03 1988-01-06 Health Lab Service Board Assay of sailicylates/reduced pyridine nucleotides
WO1990001559A1 (en) * 1988-08-02 1990-02-22 London Biotechnology Limited An amplification assay for hydrolase enzymes

Also Published As

Publication number Publication date
DE69308581D1 (de) 1997-04-10
IL107027A0 (en) 1993-12-28
DE69308581T2 (de) 1997-06-26
GR3023654T3 (en) 1997-09-30
EP0663016A1 (en) 1995-07-19
JP3229884B2 (ja) 2001-11-19
AU4827593A (en) 1994-04-12
DK0663016T3 (da) 1997-09-01
AU677223B2 (en) 1997-04-17
GB2285684A (en) 1995-07-19
WO1994006931A1 (en) 1994-03-31
JPH08503847A (ja) 1996-04-30
EP0663016B1 (en) 1997-03-05
IL107027A (en) 1997-08-14
ZA936813B (en) 1994-07-01
ATE149572T1 (de) 1997-03-15
GB2285684B (en) 1996-05-29
NZ256076A (en) 1996-01-26
GB9220027D0 (en) 1992-11-04
GB9504693D0 (en) 1995-05-03

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