ES2079467T3 - Procedimiento y dispositivo para la transformacion genetica de celulas. - Google Patents

Procedimiento y dispositivo para la transformacion genetica de celulas.

Info

Publication number
ES2079467T3
ES2079467T3 ES90810970T ES90810970T ES2079467T3 ES 2079467 T3 ES2079467 T3 ES 2079467T3 ES 90810970 T ES90810970 T ES 90810970T ES 90810970 T ES90810970 T ES 90810970T ES 2079467 T3 ES2079467 T3 ES 2079467T3
Authority
ES
Spain
Prior art keywords
cells
procedure
gold particles
atomised
genetic transformation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90810970T
Other languages
English (en)
Inventor
Christof Dr Sautter
Heinz Waldner
Ingo Prof Dr Potrykus
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis AG
Eidgenoessische Technische Hochschule Zurich ETHZ
Original Assignee
Ciba Geigy AG
Eidgenoessische Technische Hochschule Zurich ETHZ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ciba Geigy AG, Eidgenoessische Technische Hochschule Zurich ETHZ filed Critical Ciba Geigy AG
Application granted granted Critical
Publication of ES2079467T3 publication Critical patent/ES2079467T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/89—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Abstract

LA INVENCION SE REFIERE A LA TRANSFORMACION GENETICA DE CELULAS Y CONSISTE EN; PULVERIZAR UNAS GOTAS (T) DE SOLUCION DE DNA CON PARTICULAS DE ORO SUSPENDIDAS A TRAVES DE PRESIONADORES Y EN LA DESEMBOCADURA DE UNA CANULA (42) PERTENECIENTE A UNA CAMARA DE PRESION TUBULAR (17); PRESIONAR LAS GOTAS QUE LLEVAN LAS PARTICULAS SUSPENDIDAS Y LOS DNA MIGRATORIOS; ACELERAR LAS GOTAS; ENFOCAR LAS GOTAS EN EL EXTREMO DE LA CAMARA DE PRESION A TRAVES DE UNA RESTRICCION (17); HACER PASAR LAS GOTAS A TRAVES DEL ESPACIO LIBRE DE UNA CAMARA (2) DE ENSAYO DE EVACUACION; FIJAR LAS CELULAS EN UNA ZONA PUNTUAL LIMITADORA DE UN SOPORTE (25) Y HACER PENETRAR A LAS GOTAS EN EL SOPORTE.
ES90810970T 1989-12-19 1990-12-11 Procedimiento y dispositivo para la transformacion genetica de celulas. Expired - Lifetime ES2079467T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CH456289 1989-12-19

Publications (1)

Publication Number Publication Date
ES2079467T3 true ES2079467T3 (es) 1996-01-16

Family

ID=4278390

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90810970T Expired - Lifetime ES2079467T3 (es) 1989-12-19 1990-12-11 Procedimiento y dispositivo para la transformacion genetica de celulas.

Country Status (12)

Country Link
US (2) US5976880A (es)
EP (1) EP0434616B1 (es)
JP (1) JPH0568575A (es)
AT (1) ATE130371T1 (es)
AU (1) AU636422B2 (es)
CA (1) CA2032443A1 (es)
DE (1) DE59009881D1 (es)
ES (1) ES2079467T3 (es)
HU (1) HUT56141A (es)
IE (1) IE904582A1 (es)
IL (1) IL96689A0 (es)
NZ (1) NZ236486A (es)

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Also Published As

Publication number Publication date
HUT56141A (en) 1991-07-29
IL96689A0 (en) 1991-09-16
ATE130371T1 (de) 1995-12-15
JPH0568575A (ja) 1993-03-23
DE59009881D1 (de) 1995-12-21
AU6818290A (en) 1991-06-27
NZ236486A (en) 1992-04-28
US5976880A (en) 1999-11-02
EP0434616B1 (de) 1995-11-15
EP0434616A1 (de) 1991-06-26
IE904582A1 (en) 1991-06-19
AU636422B2 (en) 1993-04-29
US5877023A (en) 1999-03-02
CA2032443A1 (en) 1991-06-20

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