ES2050369T3 - Pistola de particulas mejorada. - Google Patents

Pistola de particulas mejorada.

Info

Publication number
ES2050369T3
ES2050369T3 ES90304872T ES90304872T ES2050369T3 ES 2050369 T3 ES2050369 T3 ES 2050369T3 ES 90304872 T ES90304872 T ES 90304872T ES 90304872 T ES90304872 T ES 90304872T ES 2050369 T3 ES2050369 T3 ES 2050369T3
Authority
ES
Spain
Prior art keywords
cartridge
macroprojectile
barrel
stopping
biological material
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90304872T
Other languages
English (en)
Inventor
Dwight Tomes
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pioneer Hi Bred International Inc
Original Assignee
Pioneer Hi Bred International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred International Inc filed Critical Pioneer Hi Bred International Inc
Application granted granted Critical
Publication of ES2050369T3 publication Critical patent/ES2050369T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Sustainable Development (AREA)
  • Plant Pathology (AREA)
  • Mechanical Engineering (AREA)
  • Manufacturing Of Micro-Capsules (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • General Preparation And Processing Of Foods (AREA)
  • Developing Agents For Electrophotography (AREA)

Abstract

SE PRESENTA UN CAÑON DE PARTICULAS, ADECUADO PARA TRANSFERIR MATERIALES BIOLOGICOS AL INTERIOR DEL CITOPLASMA DE CELULAS VIVAS SIN QUE SEAN TRANSMITIDOS RESTOS SIGNIFICATIVOS AL INTERIOR DE LAS CELULAS, EL CAÑON COMPRENDE MEDIOS PARA MANTENER LAS CELULAS VIVAS; UNA CAMARA DE CARTUCHO (16) EN UN EXTREMO PAR RECIBIR UN MACROPROYECTIL QUE LLEVA MATERIAL BIOLOGICO Y UN CARTUCHO DE FUEGO (24) QUE CONTIENE CARGA SUFICIENTE, CUANDO EL CARTUCHO SE DISPARA, PARA IMPULSAR EL MATERIAL BIOLOGICO AL INTERIOR DE LAS CELULAS VIVAS, UN ALMA RALLADA (26) Y UNA BOCACHA (14) EN EL OTRO EXTREMO DEL CILINDRO (12); MEDIOS PERCUTORES DE DISPARO (22) ASOCIADOS CON LA CAMARA DEL CARTUCHO (16); UNA PLANCHA DE PARO (46) POSICIONADA ENTRE EL CILINDRO Y LOS MEDIOS QUE MANTIENEN LA CELULA PARA PARAR EL MACROPROYECTIL, TENIENDO DICHOS MEDIOS DE PARO UN ORIFICIO QUE PERMITA QUE EL MATERIAL BIOLOGICO CORTADO SOBRE EL MACROPROYECTIL PASEN A TRAVES DE EL; Y UNA CAMARA DE VACIO QUE RODEA EL EXTREMO DE LA BOCACHA DEL CILINDRO, LA PLANCHA DE PARO (46) Y LOS MEDIOS PARA LA SUJECCION DE LA CELULA.
ES90304872T 1989-05-12 1990-05-04 Pistola de particulas mejorada. Expired - Lifetime ES2050369T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US35107589A 1989-05-12 1989-05-12

Publications (1)

Publication Number Publication Date
ES2050369T3 true ES2050369T3 (es) 1994-05-16

Family

ID=23379467

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90304872T Expired - Lifetime ES2050369T3 (es) 1989-05-12 1990-05-04 Pistola de particulas mejorada.

Country Status (5)

Country Link
EP (1) EP0397413B1 (es)
AT (1) ATE100138T1 (es)
DE (1) DE69005894T2 (es)
DK (1) DK0397413T3 (es)
ES (1) ES2050369T3 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5610042A (en) * 1991-10-07 1997-03-11 Ciba-Geigy Corporation Methods for stable transformation of wheat
SK115094A3 (en) * 1993-01-29 1995-07-11 Pioneer Hi Bred Int Pretreatment of microprojectiles prior to using a particle gun
DE4438232A1 (de) * 1994-10-26 1996-05-02 Guenter Prof Dr Fuhr Kryokonservierung und Tieftemperaturbearbeitung von biologischen Zellen

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3853278T2 (de) * 1988-02-29 1995-07-13 Du Pont Vorrichtung für das Liefern von Substanzen in Zellen und Gewebe auf eine nichtletale Weise.

Also Published As

Publication number Publication date
DK0397413T3 (da) 1994-02-14
DE69005894T2 (de) 1994-04-28
EP0397413B1 (en) 1994-01-12
EP0397413A1 (en) 1990-11-14
ATE100138T1 (de) 1994-01-15
DE69005894D1 (de) 1994-02-24

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