ES2079386T3 - Ensayos para beta-lactamasa que utilizan los sustratos capaces de precipitar un cromogeno. - Google Patents

Ensayos para beta-lactamasa que utilizan los sustratos capaces de precipitar un cromogeno.

Info

Publication number
ES2079386T3
ES2079386T3 ES90100438T ES90100438T ES2079386T3 ES 2079386 T3 ES2079386 T3 ES 2079386T3 ES 90100438 T ES90100438 T ES 90100438T ES 90100438 T ES90100438 T ES 90100438T ES 2079386 T3 ES2079386 T3 ES 2079386T3
Authority
ES
Spain
Prior art keywords
beta
chromogen
precipitating
assays used
substrates capable
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90100438T
Other languages
English (en)
Inventor
Christopher Bieniarz
Michael J Cornwell
Douglas F Young
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Application granted granted Critical
Publication of ES2079386T3 publication Critical patent/ES2079386T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2326/00—Chromogens for determinations of oxidoreductase enzymes
    • C12Q2326/90—Developer
    • C12Q2326/92—Nitro blue tetrazolium chloride, i.e. NBT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/978—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • G01N2333/986—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides (3.5.2), e.g. beta-lactamase (penicillinase, 3.5.2.6), creatinine amidohydrolase (creatininase, EC 3.5.2.10), N-methylhydantoinase (3.5.2.6)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2415/00—Assays, e.g. immunoassays or enzyme assays, involving penicillins or cephalosporins

Abstract

EL INVENTO CONSISTE EN SUSTRATOS PARA BETA - LACTAMASA Y LAS PRUEBAS REALIZADAS USANDO TALES SUSTRATOS.
ES90100438T 1989-01-17 1990-01-10 Ensayos para beta-lactamasa que utilizan los sustratos capaces de precipitar un cromogeno. Expired - Lifetime ES2079386T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/298,098 US4978613A (en) 1989-01-17 1989-01-17 Beta-lactamase assay employing chromogenic precipitating substrates

Publications (1)

Publication Number Publication Date
ES2079386T3 true ES2079386T3 (es) 1996-01-16

Family

ID=23149021

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90100438T Expired - Lifetime ES2079386T3 (es) 1989-01-17 1990-01-10 Ensayos para beta-lactamasa que utilizan los sustratos capaces de precipitar un cromogeno.

Country Status (6)

Country Link
US (1) US4978613A (es)
EP (1) EP0379048B1 (es)
JP (1) JPH02234699A (es)
CA (1) CA2007885A1 (es)
DE (1) DE69022255T2 (es)
ES (1) ES2079386T3 (es)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3900649A1 (de) * 1989-01-11 1990-07-12 Boehringer Mannheim Gmbh Verfahren zur abloesung eines analyten von seinem bindeprotein
US5126275A (en) * 1990-09-19 1992-06-30 Miles Inc. Analytical method using tetrazolium salt indicators having a reflectance plateau
US5338843A (en) * 1992-01-30 1994-08-16 Becton, Dickinson And Company Fluorogenic and chromogenic β-lactamase substrates
US5354658A (en) * 1993-01-28 1994-10-11 Dennis Wright Non-radioactive method for detecting a labelled segment and a solution or composition therefor
US5741657A (en) * 1995-03-20 1998-04-21 The Regents Of The University Of California Fluorogenic substrates for β-lactamase and methods of use
ATE358276T1 (de) * 1995-06-07 2007-04-15 Bioveris Corp Elektrochemilumineszenz-enzymimmunoassay
US20040157262A1 (en) * 1996-04-30 2004-08-12 Michel Kohl Conjugates of haptens to beta-lactam derivatives and their use for detecting and/or quantifying haptens in solution and device for implementation thereof
US20030235877A1 (en) * 1996-04-30 2003-12-25 La Region Wallone Conjugates of haptens to beta-lactam derivatives and their use for detecting and/or quantifying haptens in solution and device for implementation thereof
US5955604A (en) * 1996-10-15 1999-09-21 The Regents Of The University Of California Substrates for β-lactamase and uses thereof
US6225074B1 (en) 1997-08-18 2001-05-01 Dennis Wright Direct chloramphenicol acetyl transferase assay
US6031094A (en) * 1998-07-23 2000-02-29 The Regents Of The University Of California Beta-lactam substrates and uses thereof
US8148110B2 (en) * 1999-03-15 2012-04-03 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions by β-lactamase reporter fragment complementation
EP1122542A1 (en) * 2000-02-01 2001-08-08 Anda Biologicals S.A. Method for the rapid detection of whole microorganisms on retaining membranes by use of chaotropic agents
PT1799844E (pt) 2004-09-10 2010-03-29 Genencor Int Métodos para detecção de alvos
GB201017547D0 (en) * 2010-10-18 2010-12-01 Univ Cardiff Method and device for the detection of sulphur containing species
US9689021B2 (en) * 2011-10-14 2017-06-27 Université de Liège Method for measuring beta-lactam antibiotics
WO2018152443A1 (en) * 2017-02-20 2018-08-23 Regents Of The University Of California Amplification technology using dual enzyme cascade detection
US11385238B2 (en) * 2017-06-30 2022-07-12 Amgen Inc. Methods of protein clips recovery
CN107607719B (zh) * 2017-11-02 2019-08-02 北京中生金域诊断技术股份有限公司 一种测定样品中β-内酰胺酶方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3006889A1 (de) * 1980-02-23 1981-09-10 Hoechst Ag, 6000 Frankfurt Chromogene cephalosporine und verfahren zu ihrer herstellung
JPS59112973A (ja) * 1983-11-10 1984-06-29 Doujin Kagaku Kenkyusho:Kk テトラゾリウム塩化合物およびその化合物を用いる脱水素酵素の吸光光度定量法
JPS6184A (ja) * 1984-06-09 1986-01-06 Wako Pure Chem Ind Ltd 新規なテトラゾリウム化合物
IL85018A0 (en) * 1988-01-03 1988-06-30 Orgenics Ltd Stable chromogenic substrate mixture of indoxyl phosphate and tetrazolium salt,method of making and using same in biological and diagnostic assays

Also Published As

Publication number Publication date
US4978613A (en) 1990-12-18
DE69022255D1 (de) 1995-10-19
JPH02234699A (ja) 1990-09-17
EP0379048A2 (en) 1990-07-25
DE69022255T2 (de) 1996-04-18
EP0379048B1 (en) 1995-09-13
EP0379048A3 (en) 1991-10-23
CA2007885A1 (en) 1990-07-17

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