ES2074483T3 - Procedimiento de determinacion de una enzima en una mezcla de isoenzimas, asi como un soporte del test apto para el mismo y su aplicacion. - Google Patents

Procedimiento de determinacion de una enzima en una mezcla de isoenzimas, asi como un soporte del test apto para el mismo y su aplicacion.

Info

Publication number
ES2074483T3
ES2074483T3 ES90101657T ES90101657T ES2074483T3 ES 2074483 T3 ES2074483 T3 ES 2074483T3 ES 90101657 T ES90101657 T ES 90101657T ES 90101657 T ES90101657 T ES 90101657T ES 2074483 T3 ES2074483 T3 ES 2074483T3
Authority
ES
Spain
Prior art keywords
support
enzyme
application
inhibited
enzymes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90101657T
Other languages
English (en)
Inventor
Hans-Erich Dr Rer Nat Wilk
Anselm Dr Rer Nat Rothe
Erich Dr Schneider
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Application granted granted Critical
Publication of ES2074483T3 publication Critical patent/ES2074483T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/007—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving isoenzyme profiles
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/962—Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/969—Multiple layering of reactants
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/97—Test strip or test slide

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

EL INVENTO SE REFIERE A UN PROCEDIMIENTO PARA DETERMINAR UNAS ENZIMAS DE UNA MEZCLA DE ISOENZIMAS EN UNA MUESTRA LIQUIDO A TRAVES DE LA INHIBICION DE LA ISOENZIMAS PERTURBADORA Y DETERMINACION DE LAS ENZIMAS NO INHIBIDAS: TRATA DE UN SOPORTE TESTIGO APROPIADO PARA DETERMINAR LAS ISOENZIMAS Y DESCRIBE LA APLICACION DEL SOPORTE TESTIGO. SE CARACTERIZA EL PROCEDIMIENTO PORQUE LA MEZCLA ISOENZIMA CONTACTA CON UNO O VARIAS SUBSTANCIAS, QUE SON LAS ISOENZIMAS PERTTURBADORAS A INHIBIRSE. LA MUESTRA CONTENIENDO LAS SUSTANCIAS A INHIBIRSE SE SUMINISTRA EN UN MEDIO DE REACCION CON PEQUEÑOS INTERSTICIOS QUE INHIBE ALLI LA ENZIMA PERTURBADORA Y LA DETERMINACION DE LAS ENZIMAS NO INHIBIDAS SE REALIZAN EN EL LIQUIDO RESULTANTE
ES90101657T 1989-02-02 1990-01-27 Procedimiento de determinacion de una enzima en una mezcla de isoenzimas, asi como un soporte del test apto para el mismo y su aplicacion. Expired - Lifetime ES2074483T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE3903114A DE3903114A1 (de) 1989-02-02 1989-02-02 Verfahren zur bestimmung eines enzyms aus einem isoenzymgemisch sowie hierfuer geeigneter testtraeger und dessen verwendung

Publications (1)

Publication Number Publication Date
ES2074483T3 true ES2074483T3 (es) 1995-09-16

Family

ID=6373306

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90101657T Expired - Lifetime ES2074483T3 (es) 1989-02-02 1990-01-27 Procedimiento de determinacion de una enzima en una mezcla de isoenzimas, asi como un soporte del test apto para el mismo y su aplicacion.

Country Status (8)

Country Link
US (1) US5314803A (es)
EP (1) EP0381091B1 (es)
JP (1) JPH0659235B2 (es)
AT (1) ATE123313T1 (es)
AU (1) AU630147B2 (es)
CA (1) CA2009125A1 (es)
DE (2) DE3903114A1 (es)
ES (1) ES2074483T3 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9024970D0 (en) * 1990-11-16 1991-01-02 Genzyme Uk Ltd Assay
JP3124435B2 (ja) * 1993-10-20 2001-01-15 キッコーマン株式会社 α‐アミラーゼアイソザイム活性の分別定量法
DE19523049A1 (de) * 1995-06-24 1997-01-02 Boehringer Mannheim Gmbh Mehrschichtiges Analysenelement zur Bestimmung eines Analyten in einer Flüssigkeit
US5753497A (en) * 1995-12-22 1998-05-19 Universal Health Watch Inc Diagnostic assay providing blood separation
US6673629B2 (en) * 1998-01-15 2004-01-06 Abbott Laboratories Neutralization of polycations in a chromatographic device for whole blood use
JPH11299498A (ja) * 1998-02-19 1999-11-02 Toyobo Co Ltd アミラ―ゼアイソザイム活性測定用試薬
GB2470962A (en) * 2009-06-12 2010-12-15 Dna Supernova Ltd Amplification medium for use with a solid-phase substrate

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2910134A1 (de) * 1979-03-15 1980-09-25 Boehringer Mannheim Gmbh Diagnostisches mittel zum nachweis von bestandteilen von koerperfluessigkeiten
DE3029579C2 (de) * 1980-08-05 1985-12-12 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren und Mittel zur Abtrennung von Plasma oder Serum aus Vollblut
JPS57208997A (en) * 1981-06-17 1982-12-22 Fuji Photo Film Co Ltd Liquid analyzing material for oxidase enzyme reaction system
DE3342736A1 (de) * 1983-11-25 1985-06-05 Boehringer Mannheim Gmbh, 6800 Mannheim Hybridoma-antikoerper
DE3500526A1 (de) * 1985-01-09 1986-07-10 Boehringer Mannheim Gmbh, 6800 Mannheim Spezifisch hemmender s-amylase-mak
DE3508427A1 (de) * 1985-03-09 1986-09-11 Merck Patent Gmbh, 6100 Darmstadt Mittel und verfahren zur abtrennung von plasma oder serum aus vollblut
DE3511012A1 (de) * 1985-03-27 1986-10-02 Behringwerke Ag, 3550 Marburg Verfahren und testvorrichtung zur bestimmung von analyten
DE3525926A1 (de) * 1985-07-19 1987-01-29 Boehringer Mannheim Gmbh Verfahren und reagenz zur spezifischen bestimmung von pankreas-alpha-amylase
DE3621271A1 (de) * 1986-06-25 1988-01-07 Boehringer Mannheim Gmbh Verfahren und reagenz zur bestimmung von alpha-amylase
US4950592A (en) * 1987-05-12 1990-08-21 Eastman Kodak Company Blend of monoclonal antibodies
DE3740471A1 (de) * 1987-11-28 1989-06-08 Boehringer Mannheim Gmbh Testtraeger zur analyse einer probenfluessigkeit und verfahren zu seiner herstellung
US4891313A (en) * 1988-01-21 1990-01-02 Boehringer Manheim Corporation Method for determination of a component of a sample
DE3814370A1 (de) * 1988-04-28 1989-11-09 Boehringer Mannheim Gmbh Testtraeger fuer die analyse einer probenfluessigkeit, verfahren zur durchfuehrung einer solchen analyse und herstellungsverfahren

Also Published As

Publication number Publication date
JPH0659235B2 (ja) 1994-08-10
CA2009125A1 (en) 1990-08-02
US5314803A (en) 1994-05-24
EP0381091B1 (de) 1995-05-31
JPH02245199A (ja) 1990-09-28
DE3903114A1 (de) 1990-08-09
EP0381091A3 (de) 1991-09-11
AU4894290A (en) 1990-08-09
AU630147B2 (en) 1992-10-22
ATE123313T1 (de) 1995-06-15
DE59009166D1 (de) 1995-07-06
EP0381091A2 (de) 1990-08-08

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