ES2069277T3 - Cepa ral-4, util para la produccion de proteinas y peptidos pequeños. - Google Patents

Cepa ral-4, util para la produccion de proteinas y peptidos pequeños.

Info

Publication number
ES2069277T3
ES2069277T3 ES91903044T ES91903044T ES2069277T3 ES 2069277 T3 ES2069277 T3 ES 2069277T3 ES 91903044 T ES91903044 T ES 91903044T ES 91903044 T ES91903044 T ES 91903044T ES 2069277 T3 ES2069277 T3 ES 2069277T3
Authority
ES
Spain
Prior art keywords
ral
proteins
cepa
useful
production
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91903044T
Other languages
English (en)
Inventor
Robert Alan Lepley
Eric Robert Olson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pharmacia and Upjohn Co
Original Assignee
Upjohn Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Upjohn Co filed Critical Upjohn Co
Application granted granted Critical
Publication of ES2069277T3 publication Critical patent/ES2069277T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/60—Growth hormone-releasing factor [GH-RF], i.e. somatoliberin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Endocrinology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Diabetes (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

ESTA INVENCION PRESENTA UNA NUEVA DEFORMACION DE E.COLI, RAL-4, QUE ES UN AUXOTROFO DE TRIPTOFANO Y MUESTRA EXPRESION MEJORADA DE PROTEINAS Y PEPTIDOS. ADEMAS, ESTA INVENCION PRESENTA UN METODO PARA LA OBTENCION DE UNA EXPRESION MEJORADA DE PROTEINAS Y PEPTIDOS MEDIANTE EL USO DE RAL-4 O VIA GENERACION DE UNA AUXOTROFIA DE CELULA HUESPED PARA UN AMINOACIDO NO RESIDENTE EN LA PROTEINA HETEROLOGA A EXPRESAR. EN UNA REALIZACION DE LA INVENCION, IGF-I Y GRF SE EXPRESAN EN NIVELES POR ENCIMA DE LOS ENCONTRADOS EN CONTROLES DE TIPO COMUN.
ES91903044T 1990-01-31 1991-01-18 Cepa ral-4, util para la produccion de proteinas y peptidos pequeños. Expired - Lifetime ES2069277T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US47264090A 1990-01-31 1990-01-31

Publications (1)

Publication Number Publication Date
ES2069277T3 true ES2069277T3 (es) 1995-05-01

Family

ID=23876348

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91903044T Expired - Lifetime ES2069277T3 (es) 1990-01-31 1991-01-18 Cepa ral-4, util para la produccion de proteinas y peptidos pequeños.

Country Status (10)

Country Link
EP (1) EP0514405B1 (es)
JP (1) JPH05504063A (es)
AT (1) ATE116687T1 (es)
AU (2) AU643752B2 (es)
CA (1) CA2070994A1 (es)
DE (1) DE69106498T2 (es)
DK (1) DK0514405T3 (es)
ES (1) ES2069277T3 (es)
GR (1) GR3015629T3 (es)
WO (1) WO1991011523A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9712818D0 (en) * 1996-07-08 1997-08-20 Cambridge Antibody Tech Labelling and selection of specific binding molecules
US6291245B1 (en) 1998-07-15 2001-09-18 Roche Diagnostics Gmbh Host-vector system
EP0972838B1 (en) * 1998-07-15 2004-09-15 Roche Diagnostics GmbH Escherichia coli host/vector system based on antibiotic-free selection by complementation of an auxotrophy
US6656721B1 (en) 2000-08-08 2003-12-02 Roche Vitamins, Inc. Polynucleotide portions of the biotin operon from B. subtilis for use in enhanced fermentation
ATE468392T1 (de) * 2003-12-23 2010-06-15 Council Scient Ind Res Verfahren zur herstellung von streptokinase durch kultivierung von genetisch veränderten escherichia coli

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5685289A (en) * 1979-12-13 1981-07-11 Ajinomoto Co Inc Preparation of l-valine by fermentation
JPS6078590A (ja) * 1983-10-07 1985-05-04 Ajinomoto Co Inc 発酵法によるｌ−フエニルアラニンの製造法
CA1338664C (en) * 1985-04-10 1996-10-22 Cetus Oncology Corporation Recombinant hosts
JPS63503356A (ja) * 1986-05-20 1988-12-08 ザ・トラステイーズ・オブ・コロンビア・ユニヴアーシテイ・イン・ザ・シテイ・オブ・ニユー・ヨーク ヒトｔ細胞リンパ球向性ウイルス（ｈｔｌｖ‐３）逆転写酵素の発現及びその使用
WO1988006186A2 (en) * 1987-02-19 1988-08-25 The Upjohn Company cDNAs ENCODING SOMATOTROPIN, EXPRESSION VECTORS AND HOSTS

Also Published As

Publication number Publication date
GR3015629T3 (en) 1995-06-30
AU658312B2 (en) 1995-04-06
DE69106498D1 (de) 1995-02-16
DE69106498T2 (de) 1995-05-24
ATE116687T1 (de) 1995-01-15
AU7184091A (en) 1991-08-21
DK0514405T3 (da) 1995-07-10
AU643752B2 (en) 1993-11-25
JPH05504063A (ja) 1993-07-01
CA2070994A1 (en) 1991-08-01
WO1991011523A1 (en) 1991-08-08
EP0514405B1 (en) 1995-01-04
EP0514405A1 (en) 1992-11-25
AU4181593A (en) 1993-09-30

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