ES2062394T3 - Medio que contiene un ligando para la separacion cromatografica, procedimiento para la preparacion del medio y uso del medio para aislar moleculas naturales o sinteticas de una mezcla liquida. - Google Patents

Medio que contiene un ligando para la separacion cromatografica, procedimiento para la preparacion del medio y uso del medio para aislar moleculas naturales o sinteticas de una mezcla liquida.

Info

Publication number
ES2062394T3
ES2062394T3 ES90310154T ES90310154T ES2062394T3 ES 2062394 T3 ES2062394 T3 ES 2062394T3 ES 90310154 T ES90310154 T ES 90310154T ES 90310154 T ES90310154 T ES 90310154T ES 2062394 T3 ES2062394 T3 ES 2062394T3
Authority
ES
Spain
Prior art keywords
media
ligand
preparing
procedure
liquid mixture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90310154T
Other languages
English (en)
Inventor
Ferdinand Carl Haase
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rohm and Haas Co
Original Assignee
Rohm and Haas Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rohm and Haas Co filed Critical Rohm and Haas Co
Application granted granted Critical
Publication of ES2062394T3 publication Critical patent/ES2062394T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B01D15/3823—Affinity chromatography of other types, e.g. avidin, streptavidin, biotin
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3206—Organic carriers, supports or substrates
    • B01J20/3208—Polymeric carriers, supports or substrates
    • B01J20/321—Polymeric carriers, supports or substrates consisting of a polymer obtained by reactions involving only carbon to carbon unsaturated bonds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3214—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the method for obtaining this coating or impregnating
    • B01J20/3217—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond
    • B01J20/3219—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond involving a particular spacer or linking group, e.g. for attaching an active group
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3268—Macromolecular compounds
    • B01J20/3272—Polymers obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
    • B01J20/3274—Proteins, nucleic acids, polysaccharides, antibodies or antigens
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/81—Carrier - bound or immobilized peptides or proteins and the preparation thereof, e.g. biological cell or cell fragment as carrier
    • Y10S530/812—Peptides or proteins is immobilized on, or in, an organic carrier
    • Y10S530/815—Carrier is a synthetic polymer
    • Y10S530/816—Attached to the carrier via a bridging agent

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Peptides Or Proteins (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

SE PROPORCIONAN MEDIOS QUE CONTIENEN LIGACION MEJORADOS, UN METODO PARA PREPARAR LOS MEDIOS, Y USO DE LOS MEDIOS EN LA PRODUCCION DE PEPTIDOS, PROTEINAS, Y SIMILARES, MEDIANTE SEPARACION CROMATOGRAFICA. MAS ESPECIFICAMENTE, LOS MEDIOS LLEVAN PERMANENTEMENTE UNIDOS, POR MEDIO DE UN AGLOMERANTE COVALENTE A UN SUBSTRATO SOLIDO INERTE, UN LIGAZON DE POLIPEPTIDOS DE AVIDINA EN LA FORMA RENATURALIZADA DISOCIADA QUE SE UNE DE FORMA REVERSIBLE A ALGUNAS MOLECULAS TALES COMO PROTEINAS, PEPTIDOS, NUCLEOTIDOS, OLIGONUCLEOTIDOS, Y SIMILARES, Y A OTRAS MOLECULAS QUE SE UNEN MEDIANTE BIOTINILACION O POR MEDIOS DE SUS ESTRUCTURAS MICROMOLECULARES SECUNDARIAS/TERCIARIAS.
ES90310154T 1989-09-29 1990-09-17 Medio que contiene un ligando para la separacion cromatografica, procedimiento para la preparacion del medio y uso del medio para aislar moleculas naturales o sinteticas de una mezcla liquida. Expired - Lifetime ES2062394T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US41478589A 1989-09-29 1989-09-29

Publications (1)

Publication Number Publication Date
ES2062394T3 true ES2062394T3 (es) 1994-12-16

Family

ID=23642952

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90310154T Expired - Lifetime ES2062394T3 (es) 1989-09-29 1990-09-17 Medio que contiene un ligando para la separacion cromatografica, procedimiento para la preparacion del medio y uso del medio para aislar moleculas naturales o sinteticas de una mezcla liquida.

Country Status (18)

Country Link
US (1) US5276062A (es)
EP (1) EP0423938B1 (es)
JP (1) JPH03197500A (es)
KR (1) KR910006320A (es)
AT (1) ATE102074T1 (es)
BR (1) BR9004848A (es)
CA (1) CA2025487A1 (es)
DE (1) DE69007006T2 (es)
DK (1) DK0423938T3 (es)
EG (1) EG19353A (es)
ES (1) ES2062394T3 (es)
IL (1) IL95772A (es)
NO (1) NO180558C (es)
NZ (1) NZ235480A (es)
PL (1) PL287102A1 (es)
PT (1) PT95468B (es)
TR (1) TR26082A (es)
ZA (1) ZA907678B (es)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5556748A (en) * 1991-07-30 1996-09-17 Xenopore Corporation Methods of sandwich hybridization for the quantitative analysis of oligonucleotides
BE1006312A3 (fr) * 1991-11-29 1994-07-19 Univ Catholique Louvain Procede de selection de microorganismes recombinants comportant a leur surface au moins une molecule a activite enzymatique.
CN1091315A (zh) * 1992-10-08 1994-08-31 E·R·斯奎布父子公司 血纤维蛋白封闭剂组合物及其使用方法
US5512169A (en) * 1992-12-30 1996-04-30 Dow Corning Corporation Liquid column packing materials
US5503933A (en) * 1994-02-25 1996-04-02 Purdue Research Foundation Covalently bonded coatings
US5691152A (en) * 1995-11-09 1997-11-25 E. R. Squibb & Sons, Inc. Stable avidin composition
WO2001058561A1 (en) * 2000-02-09 2001-08-16 Resq Lab B.V. Packing materials for separation of biomolecules
US6638728B1 (en) * 2000-06-16 2003-10-28 Pierce Biotechnology, Inc. Coated surfaces with high capacity for capturing target molecules
US20040126890A1 (en) * 2002-06-10 2004-07-01 Gjerde Douglas T. Biomolecule open channel solid phase extraction systems and methods
US20040224329A1 (en) * 2003-05-08 2004-11-11 Gjerde Douglas T. Three-dimensional solid phase extraction surfaces
US20040224425A1 (en) * 2003-05-08 2004-11-11 Gjerde Douglas T. Biomolecule open channel solid phase extraction systems and methods
KR100988321B1 (ko) * 2003-07-26 2010-10-18 포항공과대학교 산학협력단 쿠커비투릴을 포함하는 고분자, 이를 이용한 정지상 및 컬럼
DE102008050588A1 (de) * 2008-10-09 2010-04-15 Martin-Luther-Universität Halle-Wittenberg Monolithische Säule mit immobilisiertem monomeren Avidin zur Anreicherung und Identifizierung biotinylierter Spezies
JPWO2019138957A1 (ja) * 2018-01-12 2021-01-28 株式会社カネカ アミノ基を有するリガンドの固定化方法
CN114371243A (zh) * 2021-12-28 2022-04-19 湖南中晟全肽生化有限公司 一种d-生物素的HPLC检测方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4382124B1 (en) * 1958-07-18 1994-10-04 Rohm & Haas Process for preparing macroreticular resins, copolymers and products of said process
DE2603319C3 (de) * 1976-01-29 1979-08-23 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren zur Fixierung von biologisch aktiven Proteinen an Trägern
US4656252A (en) * 1980-01-24 1987-04-07 Giese Roger W Amidobiotin compounds useful in a avidin-biotin multiple layering process
IL65131A0 (en) * 1982-02-28 1982-04-30 Yeda Res & Dev Process for the production of agarose-polyaldehyde beads and their biological applications
US4582810A (en) * 1983-09-30 1986-04-15 Becton, Dickinson And Company Immuno-agglutination particle suspensions
US4687820A (en) * 1984-08-22 1987-08-18 Cuno Incorporated Modified polypeptide supports
FR2571498B1 (fr) * 1984-10-04 1988-04-08 Immunotech Sa Procede de separation de cellules utilisant des anticorps et des billes de faible densite
US4874813A (en) * 1987-02-09 1989-10-17 Shannessy Daniel J O Proteins bound to a marker or solid phase support matrix using a hydrazone linkage
CA1320718C (en) * 1987-06-08 1993-07-27 Richard Frederick Hammen Chromatographic material
JPH0819010B2 (ja) * 1987-06-24 1996-02-28 エーザイ株式会社 光学異性体用分離剤
NL8701915A (nl) * 1987-08-14 1989-03-01 Waander Riethorst Adsorbensmateriaal en toepassing daarvan voor het isoleren van stollingsfaktoren.

Also Published As

Publication number Publication date
DK0423938T3 (da) 1994-03-28
BR9004848A (pt) 1991-09-10
IL95772A0 (en) 1991-06-30
AU6314390A (en) 1991-04-11
NO180558B (no) 1997-01-27
DE69007006T2 (de) 1994-08-25
US5276062A (en) 1994-01-04
AU629692B2 (en) 1992-10-08
JPH03197500A (ja) 1991-08-28
NZ235480A (en) 1992-12-23
KR910006320A (ko) 1991-04-29
NO180558C (no) 1997-05-07
EG19353A (en) 1994-12-30
EP0423938B1 (en) 1994-03-02
TR26082A (tr) 1994-12-15
DE69007006D1 (de) 1994-04-07
NO904160L (no) 1991-04-02
ZA907678B (en) 1991-06-26
PT95468A (pt) 1991-05-22
PT95468B (pt) 1997-07-31
CA2025487A1 (en) 1991-03-30
IL95772A (en) 1995-05-26
NO904160D0 (no) 1990-09-25
PL287102A1 (en) 1991-07-29
ATE102074T1 (de) 1994-03-15
EP0423938A1 (en) 1991-04-24

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