ES2056041T3 - Procedimiento para la deteccion de anticuerpos y antigenos. - Google Patents

Procedimiento para la deteccion de anticuerpos y antigenos.

Info

Publication number
ES2056041T3
ES2056041T3 ES93890224T ES93890224T ES2056041T3 ES 2056041 T3 ES2056041 T3 ES 2056041T3 ES 93890224 T ES93890224 T ES 93890224T ES 93890224 T ES93890224 T ES 93890224T ES 2056041 T3 ES2056041 T3 ES 2056041T3
Authority
ES
Spain
Prior art keywords
carrier
complexes
antibodies
antigens
natural
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93890224T
Other languages
English (en)
Other versions
ES2056041T1 (es
Inventor
Manfred Dr Schinkinger
Markus Dr Korger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sanochemia Pharmazeutika AG
Original Assignee
Sanochemia Pharmazeutika AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AT0232192A external-priority patent/AT399781B/de
Priority claimed from AT0006593A external-priority patent/AT402674B/de
Application filed by Sanochemia Pharmazeutika AG filed Critical Sanochemia Pharmazeutika AG
Publication of ES2056041T1 publication Critical patent/ES2056041T1/es
Application granted granted Critical
Publication of ES2056041T3 publication Critical patent/ES2056041T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G01N33/6857—Antibody fragments

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

PARA LA COMPROBACION DE UN ANTICUERPO CONTENIDO EN UNA MUESTRA, SE INTRODUCE LA MUESTRA EN UNA MEZCLA DE REACCION QUE CONTIENE UN SOPORTE EN FORMA DE PARTICULAS, EN EL CUAL ESTAN INMOVILIZADOS UN ANTIGENO ESPECIFICO PARA EL ANTICUERPO A COMPROBAR, COMO POR EJEMPLO, ANTIGENO DE VIRUS NATIVO O RECOMBINANTE, ANTIGENO DE BACTERIAS NATIVO O RECOMBINANTE, ANTIGENO DE HONGOS NATIVO O RECOMBINANTE U OTROS ANTIGENOS ESPECIFICOS DE LA CELULA NATIVOS O RECOMBINANTES, Y UN CONJUGADO DE POR LO MENOS UNA PROTEINA REACTIVA AL FRAGMENTO FC DE ANTICUERPOS Y UN INDICADOR COMBINADO A ESTE. A CONTINUACION SE INCUBA PARA FORMAR COMPLEJOS DEL CONJUGADO Y DE LOS ANTICUERPOS Y AL MISMO TIEMPO UNIR ESTOS COMPLEJOS AL SOPORTE. ACTO SEGUIDO SE SEPARAN EL SOPORTE Y LOS AGREGADOS PROCEDENTES DEL SOPORTE Y POR LO MENOS UN COMPLEJO QUE CONTIENE EL ANTICUERPO A COMPROBAR, DE LA MEZCLA DE REACCION LIQUIDA Y DE LOS COMPLEJOS QUE NO CONTIENEN EL ANTICUERPO A COMPROBAR Y SIN REACCION INMUNOQUIMICA ADICIONAL, SE COMPRUEBA LA PRESENCIA DE COMPLEJOS COMBINADOS EN EL SOPORTE A PARTIR DEL CONJUGADO Y DEL ANTICUERPO A COMPROBAR.
ES93890224T 1992-11-23 1993-11-15 Procedimiento para la deteccion de anticuerpos y antigenos. Expired - Lifetime ES2056041T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AT0232192A AT399781B (de) 1992-11-23 1992-11-23 Verfahren und reaktionsgemisch zum nachweisen von antikörpern und verfahren zum herstellen des reaktionsgemisches
AT0006593A AT402674B (de) 1993-01-18 1993-01-18 Verfahren und mittel zum nachweisen von verfahren und mittel zum nachweisen von antigenen antigenen

Publications (2)

Publication Number Publication Date
ES2056041T1 ES2056041T1 (es) 1994-10-01
ES2056041T3 true ES2056041T3 (es) 2000-08-01

Family

ID=25591311

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93890224T Expired - Lifetime ES2056041T3 (es) 1992-11-23 1993-11-15 Procedimiento para la deteccion de anticuerpos y antigenos.

Country Status (8)

Country Link
EP (1) EP0599803B1 (es)
JP (1) JPH06281652A (es)
AT (1) ATE191565T1 (es)
DE (1) DE59309999D1 (es)
DK (1) DK0599803T3 (es)
ES (1) ES2056041T3 (es)
GR (2) GR940300070T1 (es)
PT (1) PT599803E (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8323903B2 (en) 2001-10-12 2012-12-04 Life Technologies Corporation Antibody complexes and methods for immunolabeling
US20050069962A1 (en) 2001-10-12 2005-03-31 Archer Robert M Antibody complexes and methods for immunolabeling
EP1575520B1 (en) * 2002-11-26 2007-11-07 Defense Research & Development Organisation A process for preparation of an agglutination reagent for rapid detection of typhoid
DE102005001362A1 (de) * 2005-01-11 2006-07-20 Protagen Ag Verfahren und Vorrichtung zur Quantifizierung von Proteinen

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2039025T3 (es) * 1987-11-16 1993-08-16 Janssen Pharmaceutica N.V. Un agregado para determinar sustancias combinables.
AU613350B2 (en) * 1988-01-12 1991-08-01 Genelabs Technologies, Inc. Htlv-i peptide antigen and assay
GB8800702D0 (en) * 1988-01-13 1988-02-10 Nycomed As Test method & reagent kit therefor

Also Published As

Publication number Publication date
ATE191565T1 (de) 2000-04-15
PT599803E (pt) 2000-07-31
GR3033389T3 (en) 2000-09-29
EP0599803A1 (de) 1994-06-01
JPH06281652A (ja) 1994-10-07
GR940300070T1 (en) 1994-10-31
EP0599803B1 (de) 2000-04-05
DE59309999D1 (de) 2000-05-11
ES2056041T1 (es) 1994-10-01
DK0599803T3 (da) 2000-07-03

Similar Documents

Publication Publication Date Title
US5270169A (en) Detection of HLA antigen-containing immune complexes
KR960029789A (ko) 생체내 물질의 면역학적 분석용 키트 및 면역학적 분석 방법
AU629886B2 (en) Diagnostic test using chimeric antibodies
CA2130072A1 (en) Method of detecting a pathogen using a virus
DE3265768D1 (en) A method and a kit for the assay of antibodies to soluble antigens
US4693970A (en) Immunoassay using complement
DE59712595D1 (de) Homogene Nachweisverfahren zur Bestimmung von Subpopulationen eines Analyten
KR880014376A (ko) Hiv 특이 항원 영역의 모노클로날 항체 및 그 용법
ES2056041T3 (es) Procedimiento para la deteccion de anticuerpos y antigenos.
AU2375695A (en) Solid-phase filtration method for antigen and antibody assays in bloodgroup serology, and test kit
EP0348159A3 (en) Rapid stick test for detecion of antibodies in a specimen
AU8630898A (en) Antibodies and other binding molecules specific for hepatitis b viral antigens
ES2056074T3 (es) Procedimiento inmunoquimico y reactivo para la determinacion de un antigeno polivalente en una muestra liquida.
AU628293B2 (en) Self-contained multi-immunoassay diagnostic system
CA2380777A1 (en) Hepatitis c viral antigen immunoassay detection systems
KR910004228B1 (ko) 면역집합물을 사용한 다가성분의 측정방법
AU656221B2 (en) Method for immunological testing and composition and kit for use therein
Micheel et al. A solid-phase immunofluorescence assay (SIFA) using membrane filters
Lown et al. Application of the proteolytic enzyme papain in routine platelet serology
KR930000951A (ko) 진단 방법
CA2053618A1 (en) Assay of Substances Using Flow Cytometry
Micheel et al. [18] Solid-phase immunofluorescence assay (SIFA) for detection and characterization of monoclonal antibodies against soluble antigens

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 599803

Country of ref document: ES