ES2051810T3 - Fijador histologico. - Google Patents

Fijador histologico.

Info

Publication number
ES2051810T3
ES2051810T3 ES88116454T ES88116454T ES2051810T3 ES 2051810 T3 ES2051810 T3 ES 2051810T3 ES 88116454 T ES88116454 T ES 88116454T ES 88116454 T ES88116454 T ES 88116454T ES 2051810 T3 ES2051810 T3 ES 2051810T3
Authority
ES
Spain
Prior art keywords
histological
acids
fixator
osmotically
alcanols
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES88116454T
Other languages
English (en)
Inventor
Dr Barry A Siegfried
Eugene A Holland
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Application granted granted Critical
Publication of ES2051810T3 publication Critical patent/ES2051810T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N2001/305—Fixative compositions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2474/00—Immunochemical assays or immunoassays characterised by detection mode or means of detection
    • G01N2474/20—Immunohistochemistry assay

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Surgical Instruments (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

UN FIJATIVO HISTOLOGICO SIN MERCURIO Y FORMALDEHIDO. EL FIJATIVO UTILIZA COMPONENTES NO TOXICOS NORMALES QUE COMPRENDEN UNO O MAS ALCANOLES, UNO O MAS DIOLES Y TRIOLES, TALES COMO ETILENGLICOL, Y UNO O MAS ACIDOS, TALES COMO ACIDOS ACETICO Y FORMICO. EN UNA DISOLUCION ACUOSA. SE PUEDE AÑADIR UNA SAL DE UN ION METALICO, QUE TIENE UN ESTADO DE OXIDACION DE DOS POR LO MENOS, COMO MORDIENTE OPCIONAL. SE PUEDEN UTILIZAR SUSTANCIAS OSMOTICAMENTE ACTIVAS, TALES COMO CLORURO SODICO, COMO UNA OPCION CUANDO SE DESEA CONTROLAR LOS CAMBIOS DE VOLUMEN DE CELULA INDUCIDOS OSMOTICAMENTE.
ES88116454T 1987-10-09 1988-10-05 Fijador histologico. Expired - Lifetime ES2051810T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/107,026 US4946669A (en) 1987-10-09 1987-10-09 Histological fixatives

Publications (1)

Publication Number Publication Date
ES2051810T3 true ES2051810T3 (es) 1994-07-01

Family

ID=22314473

Family Applications (1)

Application Number Title Priority Date Filing Date
ES88116454T Expired - Lifetime ES2051810T3 (es) 1987-10-09 1988-10-05 Fijador histologico.

Country Status (9)

Country Link
US (1) US4946669A (es)
EP (1) EP0311035B1 (es)
JP (1) JPH076893B2 (es)
CA (1) CA1323550C (es)
DE (1) DE3873675T2 (es)
DK (1) DK167134B1 (es)
ES (1) ES2051810T3 (es)
GR (1) GR3005550T3 (es)
IE (1) IE62265B1 (es)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5104640A (en) * 1989-03-17 1992-04-14 Wescor, Inc. Fixative composition for fixing blood smears to slides
US5460797A (en) * 1991-05-08 1995-10-24 Streck Laboratories, Inc. Method for fixing tissues and cells for analysis using oxazolidine compounds
US5459073A (en) * 1991-05-08 1995-10-17 Streck Laboratories, Inc. Method and composition for preserving antigens and process for utilizing cytological material produced by same
US5260048A (en) * 1991-05-08 1993-11-09 Streck Laboratories, Inc. Tissue fixative solution and method
US5849517A (en) * 1991-05-08 1998-12-15 Streck Laboratories, Inc. Method and composition for preserving antigens and nucleic acids and process for utilizing cytological material produced by same
US5196182A (en) * 1991-05-08 1993-03-23 Streck Laboratories, Inc. Tissue fixative
EP0627879A1 (en) * 1991-09-20 1994-12-14 CAMIENER, Gerald, W. Methods and compositions with aldehyde stabilizing solution
CA2123227C (en) * 1992-09-10 2009-04-28 Wayne L. Ryan Method and composition for preserving antigens and process for utilizing cytological material produced by same
US5290706A (en) * 1992-11-10 1994-03-01 Camiener Gerald W Visualization system for retrieval, identification, and positioning of biological samples for subsequent microscopic examination
US5401625A (en) * 1993-06-24 1995-03-28 E. K. Industries, Inc. Histological composition for light microscopy
US5492837A (en) * 1993-08-27 1996-02-20 Biogenex Laboratories Mounting medium for microscope slide preparations
ES2116717T3 (es) * 1993-12-03 1998-07-16 Efh Inc Agente de conservacion biologico y anatomico, composicion para embalsamar mejorada y procedimiento asociado.
US5405606A (en) * 1993-12-03 1995-04-11 Efh, Inc. Embalming composition and method
DE4343461C1 (de) * 1993-12-20 1995-06-14 Erno Raumfahrttechnik Gmbh Verfahren zur Fixierung biologischer Präparate
US6221128B1 (en) * 1997-06-20 2001-04-24 Brij P. Giri Recovery and removal of mercury from effluent
KR20010023070A (ko) 1997-08-20 2001-03-26 더 유니버시티 오브 마이애미 고품질의 연속적인 재료 처리, 조직 고정-탈수-지방제거-함침 방법
US6311691B1 (en) * 1998-07-23 2001-11-06 Lincoln D. Russin Method for determining the shortest distance between the periphery of a lesion and the cut edge of the tissue specimen
US8015677B2 (en) * 2000-12-01 2011-09-13 Aard-Balm Limited Embalming fluid
US7138226B2 (en) 2002-05-10 2006-11-21 The University Of Miami Preservation of RNA and morphology in cells and tissues
DE60300218T2 (de) * 2003-03-05 2005-12-29 Milestone S.R.L. Fixierungslösung
FR2852392B1 (fr) * 2003-03-12 2005-07-08 Inst Claudius Regaud Composition de fixation tissulaire
WO2005054466A2 (en) * 2003-07-25 2005-06-16 Ambion, Inc. Methods and compositions for preparing rna from a fixed sample
AU2004284461A1 (en) * 2003-10-23 2005-05-06 Georgetown University Method for two- and three-dimensional microassembly of patterns and structures
US7470401B2 (en) 2003-10-24 2008-12-30 The University Of Miami Simplified tissue processing
ES2535047T3 (es) * 2004-12-17 2015-05-04 Ventana Medical Systems, Inc. Método para la desparafinación de una muestra tisular a base de una microemulsión
EP1804045B1 (de) * 2005-12-30 2014-03-12 QIAGEN GmbH Ein Verfahren und ein Kit zur Behandlung einer biologischen Probe
EP2469263A1 (en) * 2006-01-13 2012-06-27 Ventana Medical Systems, Inc. Biological sample processing composition and method
EP1965190A1 (en) * 2007-02-27 2008-09-03 Qiagen GmbH Fixation of a biological sample
EP2027922A1 (de) 2007-08-02 2009-02-25 Qiagen GmbH Verfahren und Vorrichtung für das Fixieren/Stabilisieren einer Probe
US8338130B2 (en) * 2011-02-25 2012-12-25 Medical Chemical Corporation Universal fecal fixative comprising a low molecular weight alcohol, a zinc salt and an organic acid
CN115184120A (zh) * 2014-05-28 2022-10-14 凯杰有限公司 用于含细胞液体样品的固定剂组合物
EP3329244B1 (en) 2015-07-30 2022-10-05 Qiagen GmbH Method of preparing a frozen biological sample
EP3374751B1 (en) 2015-11-13 2024-01-24 The United States of America, as represented by The Secretary, Department of Health and Human Services Fixatives and methods of use
CN107802889B (zh) * 2017-12-08 2019-11-01 上海微创心通医疗科技有限公司 一种干态动物源性胶原纤维组织材料及其制备方法
CN110864957A (zh) * 2019-11-18 2020-03-06 安徽雷根生物技术有限公司 一种不含甲醛的组织固定方法
WO2022087741A1 (en) * 2020-10-28 2022-05-05 University Health Network Fixative compositions and methods of preserving biological samples
CN116670497A (zh) * 2020-11-11 2023-08-29 乔治·梅森研究基金会股份有限公司 激光捕获显微切割可视化化学物质

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1017026A (fr) * 1950-05-03 1952-11-28 Technicon Chemical Company Liquide pour le traitement des tissus histologiques
US3546334A (en) * 1965-05-21 1970-12-08 Lerner Lab Inc Composition for fixing and protecting a smear of body cells and method of applying same
JPS5428628B2 (es) * 1972-05-27 1979-09-18
US3862300A (en) * 1973-03-07 1975-01-21 Applied Bioscience Histological fixative
US3997656A (en) * 1973-06-25 1976-12-14 Applied Bioscience Tissue staining method and composition
CA1103475A (en) * 1978-08-18 1981-06-23 Cesar Romero-Sierra Preservation of green plant tissues
US4328256A (en) * 1978-08-18 1982-05-04 Queen's University At Kingston Preservation of green plant tissues
CA1103476A (en) * 1979-01-30 1981-06-23 Cesar Romero-Sierra Preservation of douglas fir needles
US4486416A (en) * 1981-03-02 1984-12-04 Soll David B Protection of human and animal cells subject to exposure to trauma
US4404181A (en) * 1981-10-15 1983-09-13 Cambridge Chemical Products, Inc. Extended-life tissue fixative composition and method of using the same
US4493821A (en) * 1982-02-04 1985-01-15 Harrison James S Preservative and fixative preparations for biological systems
DE3238639A1 (de) * 1982-10-19 1984-04-19 Karlheinz 7900 Ulm Bachhuber Verfahren zur herstellung von duennschnitten biologischer gewebe und haertbares infiltrationsmittel fuer solche gewebe
JPS6130501A (ja) * 1984-07-20 1986-02-12 Kanegafuchi Chem Ind Co Ltd 生体の保存方法

Also Published As

Publication number Publication date
JPH01199160A (ja) 1989-08-10
JPH076893B2 (ja) 1995-01-30
EP0311035B1 (en) 1992-08-12
DE3873675D1 (de) 1992-09-17
CA1323550C (en) 1993-10-26
US4946669A (en) 1990-08-07
IE62265B1 (en) 1995-01-11
EP0311035A2 (en) 1989-04-12
DK562288D0 (da) 1988-10-07
DK562288A (da) 1989-04-10
DE3873675T2 (de) 1992-12-24
DK167134B1 (da) 1993-09-06
GR3005550T3 (es) 1993-06-07
EP0311035A3 (en) 1990-01-10
IE883004L (en) 1989-04-09

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