ES2048652B1 - Procedimientos de amplificacion de genoma para la deteccion e identificacion de secuencias genomicas relacionadas. - Google Patents
Procedimientos de amplificacion de genoma para la deteccion e identificacion de secuencias genomicas relacionadas.Info
- Publication number
- ES2048652B1 ES2048652B1 ES09201174A ES9201174A ES2048652B1 ES 2048652 B1 ES2048652 B1 ES 2048652B1 ES 09201174 A ES09201174 A ES 09201174A ES 9201174 A ES9201174 A ES 9201174A ES 2048652 B1 ES2048652 B1 ES 2048652B1
- Authority
- ES
- Spain
- Prior art keywords
- detection
- identification
- sequences
- genomic sequences
- designed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/702—Specific hybridization probes for retroviruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/702—Specific hybridization probes for retroviruses
- C12Q1/703—Viruses associated with AIDS
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/705—Specific hybridization probes for herpetoviridae, e.g. herpes simplex, varicella zoster
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- AIDS & HIV (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PROCEDIMIENTOS DE AMPLIFICACION DE GENOMA PARA LA DETECCION E IDENTIFICACION DE SECUENCIAS GENOMICAS RELACIONADAS. AL MENOS UNO DE LOS INICIADORES QUE INTERVIENEN EN LA DETECCION SE DISEÑA COMO MEZCLA SIMPLE DE OLIGONUCLEOTIDOS QUE CONTIENEN SECUENCIAS HOMOLOGAS SELECCIONADAS DE ENTRE LAS SECUENCIAS GENOMICAS RELACIONADAS QUE SE QUIEREN AMPLIFICAR, Y QUE PUEDEN CONTENER SECUENCIAS NO HOMOLOGAS Y/O ALTERACIONES RESPECTO A LAS SECUENCIAS CONOCIDAS. AL MENOS UNA DE LOS INICIADORES QUE INTERVIENEN EN LA REACCION DE IDENTIFICACION, CONSECUTIVA O NO A LA DE DETECCION, SE DISEÑA COMO MEZCLA SIMPLE DE OLIGONUCLEOTIDOS QUE TERMINAN EN SU EXTREMO 3'' EN UNA SECUENCIA ESPECIFICA DE LA SECUENCIA QUE SE QUIERE IDENTIFICAR, Y QUE PUEDEN CONTENER ALTERACIONES RESPECTO A LAS SECUENCIAS CONOCIDAS; LOS INICIADORES ESTAN DISEÑADOS DE MANERA QUE LOS FRAGMENTOS ESPECIFICOS AMPLIFICADOS SON DIFERENTES ENTRE SI POR TAMAÑO UOTRO MARCAJE FISICO O QUIMICO. LOS NUEVOS PROCEDIMIENTOS SON ESPECIALMENTE UTILES PARA LA DETECCION Y LA IDENTIFICACION DE AGENTES INFECCIOSOS RELACIONADOS EN UNA UNICA REACCION MULTIPLE.
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES09201174A ES2048652B1 (es) | 1992-06-05 | 1992-06-05 | Procedimientos de amplificacion de genoma para la deteccion e identificacion de secuencias genomicas relacionadas. |
AU43275/93A AU4327593A (en) | 1992-06-05 | 1993-06-04 | Methods for amplification of genome and mixtures of initiator oligonucleotides for the detection and identification of related genomic sequences |
PCT/ES1993/000048 WO1993025707A2 (es) | 1992-06-05 | 1993-06-04 | Procedimientos de amplificacion de genoma y mezclas de oligonucleotidos iniciadores para la deteccion y la identificacion de secuencias genomicas relacionadas |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES09201174A ES2048652B1 (es) | 1992-06-05 | 1992-06-05 | Procedimientos de amplificacion de genoma para la deteccion e identificacion de secuencias genomicas relacionadas. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2048652A1 ES2048652A1 (es) | 1994-03-16 |
ES2048652B1 true ES2048652B1 (es) | 1994-10-01 |
Family
ID=8277245
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES09201174A Expired - Fee Related ES2048652B1 (es) | 1992-06-05 | 1992-06-05 | Procedimientos de amplificacion de genoma para la deteccion e identificacion de secuencias genomicas relacionadas. |
Country Status (3)
Country | Link |
---|---|
AU (1) | AU4327593A (es) |
ES (1) | ES2048652B1 (es) |
WO (1) | WO1993025707A2 (es) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6589734B1 (en) | 1989-07-11 | 2003-07-08 | Gen-Probe Incorporated | Detection of HIV |
US5856088A (en) * | 1989-07-11 | 1999-01-05 | Gen-Probe Incorporated | Detection of human immunodeficiency virus type 1 |
CA2159103C (en) * | 1993-03-26 | 2002-03-12 | Sherrol H. Mcdonough | Detection of human immunodeficiency virus type 1 |
ES2093554B1 (es) * | 1995-02-17 | 1997-07-01 | Inst De Salud Carlos Iii | Procedimientos de amplificacion de genoma y mezclas de oligonucleotidos iniciadores para la deteccion y la identificacion de agentes infecciosos relacionados. |
US5912332A (en) * | 1996-07-26 | 1999-06-15 | Hybridon, Inc. | Affinity-based purification of oligonucleotides using soluble multimeric oligonucleotides |
DE19921419A1 (de) | 1999-05-08 | 2000-11-16 | Univ Ruprecht Karls Heidelberg | Verfahren zum spezifischen Nachweis und zur Identifizierung retroviraler Nukleinsäuren / Retroviren in einem Untersuchungsgut |
EP1659187A1 (en) * | 2004-11-18 | 2006-05-24 | bioMerieux B.V. | Nucleic acid sequences that can be used as primers and probes in the amplification and detection of HSV DNA and method for the amplification and detection of HSV DNA using a transcription based amplification |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5077192A (en) * | 1988-10-25 | 1991-12-31 | The General Hospital Corporation | Method of detecting antigenic, nucleic acid-containing macromolecular entities |
WO1991002091A1 (en) * | 1989-08-10 | 1991-02-21 | Northwestern University | Method of identifying herpesviruses and oligonucleotides for use therein |
NL9000134A (nl) * | 1990-01-19 | 1991-08-16 | Stichting Res Fonds Pathologie | Primers en werkwijze voor het detecteren van humaan papilloma virus genotypen m.b.v. pcr. |
JPH0491790A (ja) * | 1990-08-02 | 1992-03-25 | Shionogi & Co Ltd | 2段階pcr法 |
-
1992
- 1992-06-05 ES ES09201174A patent/ES2048652B1/es not_active Expired - Fee Related
-
1993
- 1993-06-04 AU AU43275/93A patent/AU4327593A/en not_active Abandoned
- 1993-06-04 WO PCT/ES1993/000048 patent/WO1993025707A2/es active Application Filing
Also Published As
Publication number | Publication date |
---|---|
WO1993025707A3 (es) | 1994-02-17 |
AU4327593A (en) | 1994-01-04 |
ES2048652A1 (es) | 1994-03-16 |
WO1993025707A2 (es) | 1993-12-23 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FD2A | Announcement of lapse in spain |
Effective date: 20141120 |