WO1993025707A3 - Procedimientos de amplificacion de genoma y mezclas de oligonucleotidos iniciadores para la deteccion y la identificacion de secuencias genomicas relacionadas - Google Patents

Procedimientos de amplificacion de genoma y mezclas de oligonucleotidos iniciadores para la deteccion y la identificacion de secuencias genomicas relacionadas Download PDF

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Publication number
WO1993025707A3
WO1993025707A3 PCT/ES1993/000048 ES9300048W WO9325707A3 WO 1993025707 A3 WO1993025707 A3 WO 1993025707A3 ES 9300048 W ES9300048 W ES 9300048W WO 9325707 A3 WO9325707 A3 WO 9325707A3
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WO
WIPO (PCT)
Prior art keywords
detection
mixtures
identification
oligonucleotides
sequences
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Application number
PCT/ES1993/000048
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English (en)
French (fr)
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WO1993025707A2 (es
Inventor
Matanzo Antonio Tenorio
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Inst De Salud Carlos Iii
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Publication date
Application filed by Inst De Salud Carlos Iii filed Critical Inst De Salud Carlos Iii
Priority to AU43275/93A priority Critical patent/AU4327593A/en
Publication of WO1993025707A2 publication Critical patent/WO1993025707A2/es
Publication of WO1993025707A3 publication Critical patent/WO1993025707A3/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/705—Specific hybridization probes for herpetoviridae, e.g. herpes simplex, varicella zoster

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • AIDS & HIV (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Las mezclas utilizadas para la reacción de detección se obtienen como suma simple de oligonucleótidos, contienen secuencias homólogas seleccionadas de entre las secuencias genómicas relacionadas y pueden contener secuencias no homólogas y/o alteraciones respecto a las secuencias conocidas. Las mezclas utilizadas para la reacción de identificación, consecutiva o no a la de detección, se obtienen como suma de oligonucleótidos, terminan en su extremo 3' en una secuencia específica de la secuencia a tipificar, están diseñadas de manera que los fragmentos específicos amplificados son diferentes entre sí por tamaño u otro marcaje físico o químico y pueden contener alteraciones respecto a las secuencias conocidas. Los nuevos procedimientos y mezclas son especialmente utilizables para la detección y tipificación de agentes infecciosos relacionados, especialmente de virus relacionados.
PCT/ES1993/000048 1992-06-05 1993-06-04 Procedimientos de amplificacion de genoma y mezclas de oligonucleotidos iniciadores para la deteccion y la identificacion de secuencias genomicas relacionadas WO1993025707A2 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU43275/93A AU4327593A (en) 1992-06-05 1993-06-04 Methods for amplification of genome and mixtures of initiator oligonucleotides for the detection and identification of related genomic sequences

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ES09201174A ES2048652B1 (es) 1992-06-05 1992-06-05 Procedimientos de amplificacion de genoma para la deteccion e identificacion de secuencias genomicas relacionadas.
ESP9201174 1992-06-05

Publications (2)

Publication Number Publication Date
WO1993025707A2 WO1993025707A2 (es) 1993-12-23
WO1993025707A3 true WO1993025707A3 (es) 1994-02-17

Family

ID=8277245

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/ES1993/000048 WO1993025707A2 (es) 1992-06-05 1993-06-04 Procedimientos de amplificacion de genoma y mezclas de oligonucleotidos iniciadores para la deteccion y la identificacion de secuencias genomicas relacionadas

Country Status (3)

Country Link
AU (1) AU4327593A (es)
ES (1) ES2048652B1 (es)
WO (1) WO1993025707A2 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5856088A (en) * 1989-07-11 1999-01-05 Gen-Probe Incorporated Detection of human immunodeficiency virus type 1
US6589734B1 (en) 1989-07-11 2003-07-08 Gen-Probe Incorporated Detection of HIV
JP4108118B2 (ja) * 1993-03-26 2008-06-25 ジェン−プローブ・インコーポレイテッド ヒト・免疫不全ウイルス１型の検出
ES2093554B1 (es) * 1995-02-17 1997-07-01 Inst De Salud Carlos Iii Procedimientos de amplificacion de genoma y mezclas de oligonucleotidos iniciadores para la deteccion y la identificacion de agentes infecciosos relacionados.
US5912332A (en) * 1996-07-26 1999-06-15 Hybridon, Inc. Affinity-based purification of oligonucleotides using soluble multimeric oligonucleotides
DE19921419A1 (de) 1999-05-08 2000-11-16 Univ Ruprecht Karls Heidelberg Verfahren zum spezifischen Nachweis und zur Identifizierung retroviraler Nukleinsäuren / Retroviren in einem Untersuchungsgut
EP1659187A1 (en) * 2004-11-18 2006-05-24 bioMerieux B.V. Nucleic acid sequences that can be used as primers and probes in the amplification and detection of HSV DNA and method for the amplification and detection of HSV DNA using a transcription based amplification

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0366448A2 (en) * 1988-10-25 1990-05-02 The General Hospital Corporation Method of detecting and identifying nucleic acid containing moieties
WO1991002091A1 (en) * 1989-08-10 1991-02-21 Northwestern University Method of identifying herpesviruses and oligonucleotides for use therein
WO1991010675A1 (en) * 1990-01-19 1991-07-25 Stichting Researchfonds Pathologie Primers and process for detecting human papillomavirus genotypes by pcr

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0491790A (ja) * 1990-08-02 1992-03-25 Shionogi & Co Ltd ２段階ｐｃｒ法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0366448A2 (en) * 1988-10-25 1990-05-02 The General Hospital Corporation Method of detecting and identifying nucleic acid containing moieties
WO1991002091A1 (en) * 1989-08-10 1991-02-21 Northwestern University Method of identifying herpesviruses and oligonucleotides for use therein
WO1991010675A1 (en) * 1990-01-19 1991-07-25 Stichting Researchfonds Pathologie Primers and process for detecting human papillomavirus genotypes by pcr

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
KIMURA ET AL: "Detection and direct typing of HSV by PCR", MED MICROBIOL IMMUNOL, vol. 179, 1990, pages 177 - 184 *
NUNBERG ET AL.: "Identification of the thymidine kinase gene of feline Herpesvirus: Use of degenerate oligonucleotides in PCR to isolate herpesvirus gene homologs", JOURNAL OF VIROLOGY, vol. 63, no. 8, August 1989 (1989-08-01), pages 3240 - 3249 *
VANDENVELDE ET AL.: "Fast multiplex PCR on boiled clinical samples for rapid viral diagnosis", JOURNAL OF VIROLOGICAL METHODS, vol. 30, no. 2, 1990, AMSTERDAM NL, pages 215 - 227 *

Also Published As

Publication number Publication date
ES2048652B1 (es) 1994-10-01
ES2048652A1 (es) 1994-03-16
WO1993025707A2 (es) 1993-12-23
AU4327593A (en) 1994-01-04

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