ES2030097T3 - Metodo para la determinacion de acido hialuronico. - Google Patents

Metodo para la determinacion de acido hialuronico.

Info

Publication number
ES2030097T3
ES2030097T3 ES198787850360T ES87850360T ES2030097T3 ES 2030097 T3 ES2030097 T3 ES 2030097T3 ES 198787850360 T ES198787850360 T ES 198787850360T ES 87850360 T ES87850360 T ES 87850360T ES 2030097 T3 ES2030097 T3 ES 2030097T3
Authority
ES
Spain
Prior art keywords
hyaluronic acid
reactant
reactants
complex
range
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES198787850360T
Other languages
English (en)
Inventor
Ragnhild Kristina Brandt
Margareta Elisabeth Hedlof
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pfizer Health AB
Original Assignee
Kabi Pharmacia AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kabi Pharmacia AB filed Critical Kabi Pharmacia AB
Application granted granted Critical
Publication of ES2030097T3 publication Critical patent/ES2030097T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/815—Test for named compound or class of compounds

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)

Abstract

METODO PARA LA DETERMINACION DE ACIDO HIALURONICO POR MEDIO DE TECNICA DE INHIBICION, DONDE ADEMAS DEL ACIDO HIALURONICO DE LA MUESTRA (ANALITO) SE HACEN REACCIONAR UN PRIMER REACTIVO QUE POSEE ESTRUCTURA DE ACIDO HIALURONICO (REACTIVO (1), DERIVADO DE ACIDO HIALURONICO) Y UN SEGUNDO REACTIVO QUE POSEE UNA ESTRUCTURA ENLAZANTE DE ACIDO HIALURONICO (REACTIVO (2)) CON OTRO PARA FORMAR UN COMPLEJO EN EL QUE LOS REACTIVOS (1) Y (2) SE MANTIENEN JUNTOS DEBIDO A AFINIDAD ESPECIFICA. EL COMPLEJO SE SEÑALA MEDIANTE UN MARCADOR QUE SE UNE COVALENTEMENTE A UNO DE LOS REACTIVOS O INDIRECTAMENTE MEDIANTE UN ANTICUERPO REACTIVO CON DICHO COMPLEJO. LOS RASGOS CARACTERISTICOS DEL METODO SON QUE (I) EL REACTIVO (2) ES UN REACTIVO QUE POSEE LA ESTRUCTURA ENLAZANTE DE ACIDO HIALURONICO DE PROTEOGLICANO DE CARTILAGO Y (II) LA REACCION DE LOS REACTIVOS (1) Y (2) ENTRE SI SE LLEVA A CABO (A) A UN PH ENTRE 5,8 A 7,3, HABIENDOSE OBTENIDO ESTE PH CON COMPONENTES TAMPONANTES AÑADIDOS EXTERNAMENTE (PAR ACIDO-BASE) QUE TIENEN UNA CAPACIDAD TAMPON DENTRO DE DICHO INTERVALO Y TIENEN UNA CONCENTRACION TOTAL SUPERIOR A 0,04 M EN LA MEZCLA DE REACCION, Y (B) A UNA TEMPERATURA ENTRE 0 Y 27 (GRADOS) C
ES198787850360T 1986-12-08 1987-11-20 Metodo para la determinacion de acido hialuronico. Expired - Lifetime ES2030097T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE8605270A SE8605270D0 (sv) 1986-12-08 1986-12-08 Hyaluronsyrabestemningsmetod och reagenssats for anvendning vid metoden

Publications (1)

Publication Number Publication Date
ES2030097T3 true ES2030097T3 (es) 1992-10-16

Family

ID=20366562

Family Applications (1)

Application Number Title Priority Date Filing Date
ES198787850360T Expired - Lifetime ES2030097T3 (es) 1986-12-08 1987-11-20 Metodo para la determinacion de acido hialuronico.

Country Status (7)

Country Link
US (1) US4826776A (es)
EP (1) EP0271461B1 (es)
JP (1) JP2726905B2 (es)
AT (1) ATE74207T1 (es)
DE (1) DE3777773D1 (es)
ES (1) ES2030097T3 (es)
SE (1) SE8605270D0 (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1319593C (en) * 1987-03-03 1993-06-29 Kenji Chichibu Method of assaying high molecular hyaluronic acid and kit of reagents for such assay
US5378637A (en) * 1987-07-07 1995-01-03 Ciba Corning Diagnostics Corp. Method for measuring hyaluronic acid
CA2005793A1 (en) * 1988-12-19 1990-06-19 Prachya Kongtawelert Method for the detection and quantification of hyaluronan
US5377124A (en) * 1989-09-20 1994-12-27 Aptix Corporation Field programmable printed circuit board
US5460944A (en) * 1991-10-28 1995-10-24 Boehringer Mannheim Gmbh Storable protein solution
US6350571B1 (en) 1996-02-01 2002-02-26 Vinata B. Lokeshwar Methods for detection and evaluation of bladder cancer
JP4529109B2 (ja) * 2001-01-12 2010-08-25 富士レビオ株式会社 ヒアルロン酸の測定方法および測定用キット
WO2002101389A1 (fr) * 2001-06-12 2002-12-19 Wako Pure Chemical Industries, Ltd. Procede de dosage de l'acide hyaluronique
ATE516498T1 (de) * 2004-05-20 2011-07-15 Wako Pure Chem Ind Ltd Verfahren zum testen von hyaluronsäure mit hyaluronsäure-bindungsprotein
EP2058659A4 (en) 2006-08-08 2009-10-21 Seikagaku Kogyo Co Ltd PROCESS FOR DETERMINING THE MOLECULAR WEIGHT OF HYALURONIC ACID

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4704356A (en) * 1984-03-27 1987-11-03 Rush-Presbyterian-St. Luke's Medical Center Method of diagnosing cartilage tissue abnormalities

Also Published As

Publication number Publication date
SE8605270D0 (sv) 1986-12-08
JP2726905B2 (ja) 1998-03-11
US4826776A (en) 1989-05-02
ATE74207T1 (de) 1992-04-15
JPS63150669A (ja) 1988-06-23
EP0271461A1 (en) 1988-06-15
DE3777773D1 (de) 1992-04-30
EP0271461B1 (en) 1992-03-25

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