ES2000089A6 - Procedimiento para la preparacion de un conjugado apropiado para la determinacion enzimoinunologica - Google Patents

Procedimiento para la preparacion de un conjugado apropiado para la determinacion enzimoinunologica

Info

Publication number
ES2000089A6
ES2000089A6 ES8600379A ES8600379A ES2000089A6 ES 2000089 A6 ES2000089 A6 ES 2000089A6 ES 8600379 A ES8600379 A ES 8600379A ES 8600379 A ES8600379 A ES 8600379A ES 2000089 A6 ES2000089 A6 ES 2000089A6
Authority
ES
Spain
Prior art keywords
enzyme
conjugate
enzyme immunoassay
conjugated reagent
reduction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES8600379A
Other languages
English (en)
Inventor
Harald Haug
Gerd Klinhammer
Johann Mattersberger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of ES2000089A6 publication Critical patent/ES2000089A6/es
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

PARA LA PREPARACION DE UN CONJUGADO APROPIADO PARA LA DETERMINACION ENZIMOINMUNOLOGICA A PARTIR DE UNA ENZIMA DE MARCACION, QUE CONTIENE UNA PORCION DE HIDRATO DE CARBONO, TAL COMO PEROXIDASA, Y UNA SUSTANCIA INMUNOLOGICAMENTE ACTIVA, ANTES O DESPUES DE LA COPULACION CON LA SUSTANCIA INMUNOLOGICAMENTE ACTIVA, CON UN METODO DE COPULACION QUE ATACA A LA PORCION PROTEINICA DE LA ENZIMA DE MARCACION, SE OXIDA LA ENZIMA DE MARCACION CON ACIDO PERYODICO O CON UNA SAL DE METAL ALCALINO DEL MISMO, EN UN MEDIO AUCOSO, Y LUEGO EL PRODUCTO DE OXIDACION SE REDUCE CON LA NABH4.
ES8600379A 1985-07-19 1986-07-18 Procedimiento para la preparacion de un conjugado apropiado para la determinacion enzimoinunologica Expired ES2000089A6 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19853525911 DE3525911A1 (de) 1985-07-19 1985-07-19 Konjugat fuer die enzymimmunobestimmung

Publications (1)

Publication Number Publication Date
ES2000089A6 true ES2000089A6 (es) 1987-12-01

Family

ID=6276256

Family Applications (1)

Application Number Title Priority Date Filing Date
ES8600379A Expired ES2000089A6 (es) 1985-07-19 1986-07-18 Procedimiento para la preparacion de un conjugado apropiado para la determinacion enzimoinunologica

Country Status (7)

Country Link
US (1) US4843001A (es)
EP (1) EP0209155B1 (es)
JP (1) JPS6222065A (es)
AT (1) ATE58246T1 (es)
CA (1) CA1263601A (es)
DE (2) DE3525911A1 (es)
ES (1) ES2000089A6 (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5248592A (en) * 1987-06-04 1993-09-28 Boehringer Mannheim Gmbh Use of a derivatized alkaline phosphatase as a standard
US5324650A (en) * 1990-03-20 1994-06-28 E. I. Du Pont De Nemours And Company Situ process for production of conjugates
DE4108748A1 (de) * 1991-03-18 1992-09-24 Boehringer Mannheim Gmbh Diagnose von bakteriellen infektionen durch nachweis von glykokonjugaten
DE4227102C2 (de) * 1992-08-17 2002-11-14 Dade Behring Marburg Gmbh Immunchemisches Verfahren zum Nachweis eines Analyten
CA2104413C (en) * 1992-09-04 1996-05-21 Dean William Schroer Intrinsic factor - horse radish peroxidase conjugates
DE4237479A1 (de) * 1992-11-06 1994-05-11 Behringwerke Ag Verfahren zur Herstellung von Konjugaten aus einem spezifischen Bindungspartner und einem kohlenhydrathaltigen Protein
FR2729760B1 (fr) * 1995-01-23 1997-08-14 Pasteur Sanofi Diagnostics Conjugue immunoenzymatique, son procede de preparation et ses applications
FR2734365B1 (fr) * 1995-05-18 1999-04-09 Pasteur Sanofi Diagnostics Conjugue immunoenzymatique, son procede de preparation et ses applications
DK0752102T3 (da) * 1995-01-23 2001-01-08 Pasteur Sanofi Diagnostics Immunoenzymatisk konjugat, fremgangsmåde til fremstilling af konjugatet og anvendelse af dette.
US5795784A (en) 1996-09-19 1998-08-18 Abbott Laboratories Method of performing a process for determining an item of interest in a sample
US5856194A (en) 1996-09-19 1999-01-05 Abbott Laboratories Method for determination of item of interest in a sample

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2923139A1 (de) * 1979-06-07 1980-12-18 Boehringer Mannheim Gmbh Verfahren zur herstellung von immunologisch aktiven enzymmarkierten konjugaten
US4410633A (en) * 1980-09-25 1983-10-18 Corning Glass Works Method for the measurement of free thyroxine or 3,5,3'-triiodothyronine in a liquid sample
JPS58122459A (ja) * 1982-01-14 1983-07-21 Yatoron:Kk 酵素の会合を利用した測定方法

Also Published As

Publication number Publication date
EP0209155B1 (de) 1990-11-07
CA1263601A (en) 1989-12-05
EP0209155A1 (de) 1987-01-21
US4843001A (en) 1989-06-27
DE3525911A1 (de) 1987-01-29
JPH0476579B2 (es) 1992-12-04
ATE58246T1 (de) 1990-11-15
JPS6222065A (ja) 1987-01-30
DE3675447D1 (de) 1990-12-13

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20050719