EP4741513A2 - Verfahren und zusammensetzungen zur in-situ-synchronisierung von reaktionen - Google Patents
Verfahren und zusammensetzungen zur in-situ-synchronisierung von reaktionenInfo
- Publication number
- EP4741513A2 EP4741513A2 EP26155985.0A EP26155985A EP4741513A2 EP 4741513 A2 EP4741513 A2 EP 4741513A2 EP 26155985 A EP26155985 A EP 26155985A EP 4741513 A2 EP4741513 A2 EP 4741513A2
- Authority
- EP
- European Patent Office
- Prior art keywords
- probe
- nucleic acid
- polymerase
- sample
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163227830P | 2021-07-30 | 2021-07-30 | |
| EP24186285.3A EP4450640B1 (de) | 2021-07-30 | 2022-07-29 | Verfahren und zusammensetzungen zur in-situ-synchronisierung von reaktionen |
| PCT/US2022/038915 WO2023009842A1 (en) | 2021-07-30 | 2022-07-29 | Methods and compositions for synchronizing reactions in situ |
| EP22757777.2A EP4326900B1 (de) | 2021-07-30 | 2022-07-29 | Verfahren und zusammensetzungen zur in-situ-synchronisierung von reaktionen |
Related Parent Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP22757777.2A Division EP4326900B1 (de) | 2021-07-30 | 2022-07-29 | Verfahren und zusammensetzungen zur in-situ-synchronisierung von reaktionen |
| EP24186285.3A Division-Into EP4450640B1 (de) | 2021-07-30 | 2022-07-29 | Verfahren und zusammensetzungen zur in-situ-synchronisierung von reaktionen |
| EP24186285.3A Division EP4450640B1 (de) | 2021-07-30 | 2022-07-29 | Verfahren und zusammensetzungen zur in-situ-synchronisierung von reaktionen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| EP4741513A2 true EP4741513A2 (de) | 2026-05-13 |
Family
ID=83004727
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP22757777.2A Active EP4326900B1 (de) | 2021-07-30 | 2022-07-29 | Verfahren und zusammensetzungen zur in-situ-synchronisierung von reaktionen |
| EP26155985.0A Pending EP4741513A2 (de) | 2021-07-30 | 2022-07-29 | Verfahren und zusammensetzungen zur in-situ-synchronisierung von reaktionen |
| EP24186285.3A Active EP4450640B1 (de) | 2021-07-30 | 2022-07-29 | Verfahren und zusammensetzungen zur in-situ-synchronisierung von reaktionen |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP22757777.2A Active EP4326900B1 (de) | 2021-07-30 | 2022-07-29 | Verfahren und zusammensetzungen zur in-situ-synchronisierung von reaktionen |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP24186285.3A Active EP4450640B1 (de) | 2021-07-30 | 2022-07-29 | Verfahren und zusammensetzungen zur in-situ-synchronisierung von reaktionen |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US12360105B2 (de) |
| EP (3) | EP4326900B1 (de) |
| CN (1) | CN117730161B (de) |
| AU (2) | AU2022317053B2 (de) |
| CA (1) | CA3224093A1 (de) |
| ES (1) | ES2988400T3 (de) |
| WO (1) | WO2023009842A1 (de) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA3078158A1 (en) | 2017-10-06 | 2019-04-11 | Cartana Ab | Rna templated ligation |
| GB201919032D0 (en) | 2019-12-20 | 2020-02-05 | Cartana Ab | Method of detecting an analyte |
| US12209273B2 (en) | 2020-06-12 | 2025-01-28 | 10X Genomics, Inc. | Nucleic acid assays using click chemistry bioconjugation |
| US12297499B2 (en) | 2020-08-17 | 2025-05-13 | 10X Genomics, Inc. | Multicomponent nucleic acid probes for sample analysis |
| US12509717B2 (en) | 2020-12-11 | 2025-12-30 | 10X Genomics, Inc. | Methods and compositions for multimodal in situ analysis |
| US12275984B2 (en) | 2021-03-02 | 2025-04-15 | 10X Genomics, Inc. | Sequential hybridization and quenching |
| CA3224093A1 (en) | 2021-07-30 | 2023-02-02 | Jorge Ivan Hernandez Neuta | Methods and compositions for synchronizing reactions in situ |
| US12529096B2 (en) | 2021-08-03 | 2026-01-20 | 10X Genomics, Inc. | Stabilization and/or compaction of nucleic acid structures |
| WO2023015192A1 (en) | 2021-08-03 | 2023-02-09 | 10X Genomics, Inc. | Nucleic acid concatemers and methods for stabilizing and/or compacting the same |
| WO2023023484A1 (en) | 2021-08-16 | 2023-02-23 | 10X Genomics, Inc. | Probes comprising a split barcode region and methods of use |
| WO2023172915A1 (en) | 2022-03-08 | 2023-09-14 | 10X Genomics, Inc. | In situ code design methods for minimizing optical crowding |
| AU2023243205A1 (en) * | 2022-04-01 | 2024-09-19 | 10X Genomics, Inc. | Compositions and methods for targeted masking of autofluorescence |
| US12559791B2 (en) | 2022-05-06 | 2026-02-24 | 10X Genomics, Inc. | Methods and compositions for in situ analysis of V(D)J sequences |
| WO2023220300A1 (en) | 2022-05-11 | 2023-11-16 | 10X Genomics, Inc. | Compositions and methods for in situ sequencing |
| EP4540607A1 (de) | 2022-06-17 | 2025-04-23 | 10X Genomics, Inc. | Katalytische entvernetzung von proben zur in-situ-analyse |
| WO2025029831A1 (en) | 2023-07-31 | 2025-02-06 | 10X Genomics, Inc. | Methods and systems for targeted rna cleavage and target rna-primed rolling circle amplification |
| CN119040442B (zh) * | 2024-10-31 | 2025-01-21 | 北京百力格生物科技有限公司 | 一种pcr扩增方法及其试剂盒 |
| WO2026096758A1 (en) * | 2024-11-01 | 2026-05-07 | 10X Genomics, Inc. | Methods and compositions for amplification reactions in situ |
| CN119530350B (zh) * | 2024-11-28 | 2025-09-26 | 浙江大学 | 一种可实现细胞内目标核酸特定修饰可视化的原位成像方法 |
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