EP4728092A1 - Bestimmung von modifizierten cytosinen - Google Patents

Bestimmung von modifizierten cytosinen

Info

Publication number
EP4728092A1
EP4728092A1 EP24735888.0A EP24735888A EP4728092A1 EP 4728092 A1 EP4728092 A1 EP 4728092A1 EP 24735888 A EP24735888 A EP 24735888A EP 4728092 A1 EP4728092 A1 EP 4728092A1
Authority
EP
European Patent Office
Prior art keywords
strand
polynucleotide
hairpin
sequence
library
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP24735888.0A
Other languages
English (en)
French (fr)
Inventor
Colin Brown
Sarah SHULTZABERGER
Aathavan KARUNAKARAN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Illumina Inc
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of EP4728092A1 publication Critical patent/EP4728092A1/de
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP24735888.0A 2023-06-14 2024-06-13 Bestimmung von modifizierten cytosinen Pending EP4728092A1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363508166P 2023-06-14 2023-06-14
PCT/EP2024/066447 WO2024256581A1 (en) 2023-06-14 2024-06-13 Determination of modified cytosines

Publications (1)

Publication Number Publication Date
EP4728092A1 true EP4728092A1 (de) 2026-04-22

Family

ID=91664534

Family Applications (1)

Application Number Title Priority Date Filing Date
EP24735888.0A Pending EP4728092A1 (de) 2023-06-14 2024-06-13 Bestimmung von modifizierten cytosinen

Country Status (2)

Country Link
EP (1) EP4728092A1 (de)
WO (1) WO2024256581A1 (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025254779A1 (en) * 2024-06-06 2025-12-11 Epicrispr Biotechnologies, Inc. Engineered epigenetic effectors

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020055100A1 (en) 1997-04-01 2002-05-09 Kawashima Eric H. Method of nucleic acid sequencing
JP2002503954A (ja) 1997-04-01 2002-02-05 グラクソ、グループ、リミテッド 核酸増幅法
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
EP1301591A4 (de) 2000-07-13 2004-05-26 Invitrogen Corp Verfahren und zusammensetzungen zur schnellen protein- und peptidextraktion und -isolierung mittels lysismatrize
KR20050008651A (ko) 2002-03-05 2005-01-21 솔렉사 리미티드 표현형과 관련된 게놈 와이드 서열 변형을 검출하는 방법
WO2005042781A2 (en) 2003-10-31 2005-05-12 Agencourt Personal Genomics Corporation Methods for producing a paired tag from a nucleic acid sequence and methods of use thereof
GB0400584D0 (en) 2004-01-12 2004-02-11 Solexa Ltd Nucleic acid chacterisation
ES2404311T3 (es) 2005-04-12 2013-05-27 454 Life Sciences Corporation Métodos para determinar variantes de secuencias usando secuenciación ultraprofunda
US7601499B2 (en) 2005-06-06 2009-10-13 454 Life Sciences Corporation Paired end sequencing
US8428882B2 (en) 2005-06-14 2013-04-23 Agency For Science, Technology And Research Method of processing and/or genome mapping of diTag sequences
GB0514910D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Method for sequencing a polynucleotide template
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
DK1987159T4 (da) 2006-02-08 2020-11-16 Illumina Cambridge Ltd Fremgangsmåde til sekventering af en polynukleotidtemplate
WO2007107710A1 (en) 2006-03-17 2007-09-27 Solexa Limited Isothermal methods for creating clonal single molecule arrays
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
EP2718465B1 (de) 2011-06-09 2022-04-13 Illumina, Inc. Verfahren zur vorbereitung eines analyt-arrays
CA2859660C (en) 2011-09-23 2021-02-09 Illumina, Inc. Methods and compositions for nucleic acid sequencing
WO2013138644A2 (en) 2012-03-15 2013-09-19 New England Biolabs, Inc. Methods and compositions for discrimination between cytosine and modifications thereof, and for methylome analysis
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
US20150011396A1 (en) * 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
WO2017075436A1 (en) 2015-10-30 2017-05-04 New England Biolabs, Inc. Compositions and methods for determining modified cytosines by sequencing
US11378544B2 (en) 2018-01-08 2022-07-05 Illumina, Inc. High-throughput sequencing with semiconductor-based detection
CN114502742A (zh) * 2019-08-01 2022-05-13 特温斯特兰德生物科学有限公司 用于核酸测序及相关应用的方法和试剂
US11359238B2 (en) * 2020-03-06 2022-06-14 Singular Genomics Systems, Inc. Linked paired strand sequencing
IL302207A (en) 2020-10-21 2023-06-01 Illumina Inc Sequencing templates comprising multiple inserts and compositions and methods for improving sequencing throughput
WO2023034814A1 (en) * 2021-09-03 2023-03-09 Singular Genomics Systems, Inc. Methods for differentiating modified nucleobases

Also Published As

Publication number Publication date
WO2024256581A1 (en) 2024-12-19

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