EP4728091A1 - Gleichzeitige sequenzierung mit räumlich getrennten ringen - Google Patents

Gleichzeitige sequenzierung mit räumlich getrennten ringen

Info

Publication number
EP4728091A1
EP4728091A1 EP24734826.1A EP24734826A EP4728091A1 EP 4728091 A1 EP4728091 A1 EP 4728091A1 EP 24734826 A EP24734826 A EP 24734826A EP 4728091 A1 EP4728091 A1 EP 4728091A1
Authority
EP
European Patent Office
Prior art keywords
immobilised
proportion
primers
solid support
immobilised primers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP24734826.1A
Other languages
English (en)
French (fr)
Inventor
Aathavan KARUNAKARAN
Jonathan Boutell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Illumina Inc
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of EP4728091A1 publication Critical patent/EP4728091A1/de
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP24734826.1A 2023-06-14 2024-06-13 Gleichzeitige sequenzierung mit räumlich getrennten ringen Pending EP4728091A1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363508070P 2023-06-14 2023-06-14
PCT/EP2024/066446 WO2024256580A1 (en) 2023-06-14 2024-06-13 Concurrent sequencing with spatially separated rings

Publications (1)

Publication Number Publication Date
EP4728091A1 true EP4728091A1 (de) 2026-04-22

Family

ID=91616878

Family Applications (1)

Application Number Title Priority Date Filing Date
EP24734826.1A Pending EP4728091A1 (de) 2023-06-14 2024-06-13 Gleichzeitige sequenzierung mit räumlich getrennten ringen

Country Status (2)

Country Link
EP (1) EP4728091A1 (de)
WO (1) WO2024256580A1 (de)

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
JP2002503954A (ja) 1997-04-01 2002-02-05 グラクソ、グループ、リミテッド 核酸増幅法
US20020055100A1 (en) 1997-04-01 2002-05-09 Kawashima Eric H. Method of nucleic acid sequencing
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
EP1301591A4 (de) 2000-07-13 2004-05-26 Invitrogen Corp Verfahren und zusammensetzungen zur schnellen protein- und peptidextraktion und -isolierung mittels lysismatrize
KR20050008651A (ko) 2002-03-05 2005-01-21 솔렉사 리미티드 표현형과 관련된 게놈 와이드 서열 변형을 검출하는 방법
WO2005042781A2 (en) 2003-10-31 2005-05-12 Agencourt Personal Genomics Corporation Methods for producing a paired tag from a nucleic acid sequence and methods of use thereof
GB0400584D0 (en) 2004-01-12 2004-02-11 Solexa Ltd Nucleic acid chacterisation
WO2006084132A2 (en) 2005-02-01 2006-08-10 Agencourt Bioscience Corp. Reagents, methods, and libraries for bead-based squencing
ES2404311T3 (es) 2005-04-12 2013-05-27 454 Life Sciences Corporation Métodos para determinar variantes de secuencias usando secuenciación ultraprofunda
US7601499B2 (en) 2005-06-06 2009-10-13 454 Life Sciences Corporation Paired end sequencing
US8428882B2 (en) 2005-06-14 2013-04-23 Agency For Science, Technology And Research Method of processing and/or genome mapping of diTag sequences
GB0514910D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Method for sequencing a polynucleotide template
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
DK1987159T4 (da) 2006-02-08 2020-11-16 Illumina Cambridge Ltd Fremgangsmåde til sekventering af en polynukleotidtemplate
WO2007107710A1 (en) 2006-03-17 2007-09-27 Solexa Limited Isothermal methods for creating clonal single molecule arrays
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
JP5794572B2 (ja) 2008-10-24 2015-10-14 エピセンター テクノロジーズ コーポレイションＥｐｉｃｅｎｔｒｅ Ｔｅｃｈｎｏｌｏｇｉｅｓ Ｃｏｒｐｏｒａｔｉｏｎ 核酸を修飾するためのトランスポゾン末端組成物及び方法
US9347092B2 (en) * 2009-02-25 2016-05-24 Roche Molecular System, Inc. Solid support for high-throughput nucleic acid analysis
US9005935B2 (en) 2011-05-23 2015-04-14 Agilent Technologies, Inc. Methods and compositions for DNA fragmentation and tagging by transposases
EP2718465B1 (de) 2011-06-09 2022-04-13 Illumina, Inc. Verfahren zur vorbereitung eines analyt-arrays
CA2859660C (en) 2011-09-23 2021-02-09 Illumina, Inc. Methods and compositions for nucleic acid sequencing
WO2013085918A1 (en) 2011-12-05 2013-06-13 The Regents Of The University Of California Methods and compostions for generating polynucleic acid fragments
US9670529B2 (en) * 2012-02-28 2017-06-06 Population Genetics Technologies Ltd. Method for attaching a counter sequence to a nucleic acid sample
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
DK3218511T3 (da) * 2014-11-11 2020-07-20 Illumina Cambridge Ltd Fremgangsmåder og arrays til frembringelse og sekventering af monoklonale klynger af nukleinsyre
WO2016189331A1 (en) 2015-05-28 2016-12-01 Illumina Cambridge Limited Surface-based tagmentation
US11378544B2 (en) 2018-01-08 2022-07-05 Illumina, Inc. High-throughput sequencing with semiconductor-based detection
FI3794012T3 (fi) 2018-05-15 2024-01-08 Illumina Inc Koostumuksia ja menetelmiä pintaan sitoutuneiden oligonukleotidien kemiallista pilkkomista ja suojauksen poistoa varten
IL302207A (en) 2020-10-21 2023-06-01 Illumina Inc Sequencing templates comprising multiple inserts and compositions and methods for improving sequencing throughput

Also Published As

Publication number Publication date
WO2024256580A1 (en) 2024-12-19

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