EP4361237A1 - Wäschewaschzusammensetzung - Google Patents

Wäschewaschzusammensetzung Download PDF

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Publication number
EP4361237A1
EP4361237A1 EP22204178.2A EP22204178A EP4361237A1 EP 4361237 A1 EP4361237 A1 EP 4361237A1 EP 22204178 A EP22204178 A EP 22204178A EP 4361237 A1 EP4361237 A1 EP 4361237A1
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EP
European Patent Office
Prior art keywords
composition
laundry
detergent
laundry washing
alkyl
Prior art date
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Pending
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EP22204178.2A
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English (en)
French (fr)
Inventor
Viktor Andreevich Filatov
Elena Yur'evna BELOUS
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Skylab Ag
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Skylab Ag
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Priority to EP22204178.2A priority Critical patent/EP4361237A1/de
Priority to PCT/EP2023/078307 priority patent/WO2024088769A1/en
Publication of EP4361237A1 publication Critical patent/EP4361237A1/de
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
    • C11D1/02—Anionic compounds
    • C11D1/04—Carboxylic acids or salts thereof
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
    • C11D1/02—Anionic compounds
    • C11D1/12—Sulfonic acids or sulfuric acid esters; Salts thereof
    • C11D1/14—Sulfonic acids or sulfuric acid esters; Salts thereof derived from aliphatic hydrocarbons or mono-alcohols
    • C11D1/146—Sulfuric acid esters
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
    • C11D1/02—Anionic compounds
    • C11D1/12—Sulfonic acids or sulfuric acid esters; Salts thereof
    • C11D1/29—Sulfates of polyoxyalkylene ethers
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D10/00—Compositions of detergents, not provided for by one single preceding group
    • C11D10/04—Compositions of detergents, not provided for by one single preceding group based on mixtures of surface-active non-soap compounds and soap
    • C11D10/042—Compositions of detergents, not provided for by one single preceding group based on mixtures of surface-active non-soap compounds and soap based on anionic surface-active compounds and soap
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D2111/00—Cleaning compositions characterised by the objects to be cleaned; Cleaning compositions characterised by non-standard cleaning or washing processes
    • C11D2111/10—Objects to be cleaned
    • C11D2111/12—Soft surfaces, e.g. textile

Definitions

  • the invention relates to a biodegradable deoxyribonuclease-based composition, particularly a laundry washing composition, for the breakdown of hard-to-remove fixed skin sebum and regulation of kinetics of sweat crystal formation on fabrics of various materials.
  • the composition is intended to be included in detergents for fabric care, application of which increases efficiency of removal of fixed biocontaminations and human biostains enriched with nucleic acids, as well as regulation of sweat crystallization and decreases intensity of unpleasant smells.
  • the composition is biodegradable, has a safe effect on the skin and can be used to prepare household detergents for sensitive skin, such as dry, liquid, concentrated laundry detergents, surface washes, with lasting cleanliness and a pleasant fragrance.
  • the use of the composition may help to reduce the allergen load on the consumer caused by the specific effect on the bacterial flora.
  • proteases and nucleases have been investigated to achieve a high cleaning capacity of household cleaners.
  • their combined use to date has presented a number of challenges of a biochemical nature, as proteases cleave peptide bonds in proteins and nucleases cleave phosphodiester bonds in nucleic acids (Veluchamy et al., 2011; Morales-Garcia et al., 2020).
  • Subtilisin is a proteolytic enzyme of the serine proteinase class. It is produced by bacteria mainly of the genus Bacillus, particularly Bacillus subtilis, to protect against other microorganisms. Subtilisin is involved in the cleavage of proteins through the hydrolysis of peptide bonds. It initiates a nucleophilic attack on the peptide bond via a serine residue that is located in its active site (Marget et al., 2022). The enzyme has antimicrobial and fungicidal properties and inhibits the formation of microbial biofilms. Subtilisin is widely used in household chemicals, cosmetics, food processing and pharmaceuticals (Veluchamy et al., 2011).
  • As subtilisin is a protease that cleaves proteins, including nucleases, it is used in stability experiments.
  • Nucleases are a broad group of enzymes that cleave phosphodiester bonds between nucleic acid subunits.
  • RNases ribonucleases
  • DNases deoxyribonucleases
  • Taniuchi et al. investigated the stability of the staphylococal nuclease and used subtilisin along with other nucleases. The authors showed that subtilisin cleaves the N-terminus of the nuclease (residues 1 to 5) (Taniuchi et al., 1969). Subtilisin can not only cleave nucleases but also inhibit the nuclease activity of enzymes. Yu et al. investigated the activity of the enzyme RecB, which has chelicase and nuclease activities.
  • subtilisin cleaves proteobacterium Alteromonas espejiana F and S nucleases and critically reduces their exonuclease activities against duplex DNA (Hauser et al., 1990). All the mentioned nucleases have DNAase activity, as does the nuclease described in the present patent application.
  • subtilisin and deoxyribonuclease Despite the conflict between subtilisin and deoxyribonuclease described in the literature, the authors of the invention unexpectedly found a synergistic effect between subtilisin and bionuclease derived from fungi and yeast-like fungi if, and only if, a surfactant as defined as per component C) in claim 1 of the attached claim set is added.
  • a surfactant as defined as per component C) in claim 1 of the attached claim set is added.
  • the technical result of the innovative combination or complex based on nuclease and protease consists in effective removal of biostains and regulation of kinetics of crystal formation of sweat enriched with nucleic acids, removal of unpleasant smells and keeping enzymatic stability of enzymes in combined presence in laundry detergents compositions.
  • the complex is active over a wide pH range, in particular at a pH of 6.0-11.0, and over a wide temperature range, in particular from +15 to +60 °C, which broadens the scope of application in eco-friendly products to preserve the planet's resources.
  • nuclease and protease were found to increase the removal efficiency of complex biological and protein soiling on various fabrics, in particular cotton, synthetic, mixed, delicate fabrics, as well as to increase the detergent efficiency by more than 15% on various fabrics while maintaining surfactant content based on components of natural origin.
  • Nuclease and protease are understood to be raw materials containing the respective pure active ingredients as well as technical impurities that may have formed during production of the target raw materials.
  • Nuclease produced by modern non-GMO biotechnology methods, is an active enzyme that cleaves insoluble nucleic substrates with phosphorus diether bonds.
  • Protease acts as an additive enzyme to break down peptide bonds in the most common proteinaceous contaminants, as well as in exfoliated or peeled epidermal cells.
  • An additional property is the regulation of the kinetics of nucleic acid enriched sweat crystal formation, for deep hygienic cleansing and whitening of clothes.
  • odourous aromatic substances represented by different classes of organic substances: aldehydes and ketones, terpenes, amines, indoles, sulphur-containing components (mercaptans), organic acids and their esters, phenols and cresols.
  • the innovative complex according to claim 1, comprising in particular nuclease and protease (subtilisin), aims at effective cleaning and removal of biocontamination through regulation of reaction kinetics of nucleic acids breakdown and sweat crystals formation, increasing washing efficiency of household cleaning products and neutralization of unpleasant odours.
  • the complex based on natural and biodegradable components is effective in a pH range of 6.0-11.0 in the presence of different synthetic and natural components, that allows its use as a part of wide range of household chemistry products for washing of different fabrics.
  • the components target complex biological contaminations on various fabrics and also bind excreted metabolic products with an unpleasant aroma.
  • the combined use of the components of the claimed composition in a single agent leads to an increase in the kinetics of the enzymatic cleavage reaction of complex biological contaminants by increasing the activity of nuclease due to protease (subtilisin), which allows for fast spot removal even in conditions of cold water.
  • protease subtilisin
  • Aggressive inorganic surfactants and organic solvents, occlusive film-formers and alcohols, quaternary ammonium bases, silicones, optical brighteners, chlorine and phosphorus derivatives are absent in the composition, so it is possible to use detergents and cleaners with this composition on a regular basis without any harm to human skin.
  • the combined use of these ingredients has a synergistic effect, providing complete care for different fabrics in a single household detergent for everyday use.
  • the invention as a whole relates to the composition and its application, allowing to achieve technical results such as effective enzymatic biostain cleavage with simultaneous effective neutralisation of unpleasant odours on various types of fabrics and regulation of kinetics of crystallisation of sweat enriched with nucleic acids, with maintenance of long-term cleanliness and colour of fabrics in coordinates ⁇ L*a*b, which are not achieved or not sufficiently achieved by modern commercially available means in the present technical field.
  • the invention relates to a composition intended for use in laundry detergents and is active at pH of 5.5-10.5 and a water hardness of 0-15.0 °dH, corresponding to " soft" and “medium” hardness (while higher water hardness is not excluded, even though less desired), consisting of:
  • the invention relates to the use of the composition according to the invention in a household detergent for washing laundry.
  • the household detergent according to the invention may contain 0.50 to 25.50 wt.% of the composition according to the invention.
  • the composition may differ in that said nuclease is in a water-glycerol or water-sorbitol or water-glycerol-sorbitol or water-propylene glycol solution, or in a powdered or granulated form with various excipients.
  • the dry nuclease is a commercially available product Pristine ® 100T (https://biosolutions.novozymes.com/en/pristine/products/pristine-100-t).
  • composition may be characterized in that said aqueous-glycerol nuclease solution is the commercially available product Pristine ® 100L.
  • Pristine ® 100L is available in particular from Novozymes and can be identified, for example, by ⁇ https://biosolutions.novozymes.com/en/pristine/products/pristine-100-1>.
  • composition may be characterized in that said aqueous-sorbitol nuclease solution is a commercially available Pristine ® 100L product modified with an additional amount of sorbitol.
  • the composition may be characterized in that it additionally contains an alkyl polyethylene glycol sulfate with the general formula R 1 -O(-CH 2 -CH 2 -O)n 1 (SO 3 ) n 2 X 1 , wherein n 1 takes a value from 0 to 10 and denotes the number of polyethylene groups, R 1 represents an alkyl and/or alkenyl group with a hydrocarbon chain length of 5 to 22 carbon atoms, n 2 takes a value from 0 to 1 and denotes the number of sulfate groups, X 1 is an alkali and/or alkaline earth metal cation, ammonium, alkylammonium, alkanolammonium, glucoammonium, basic amino acid.
  • R 1 takes a value from 0 to 10 and denotes the number of polyethylene groups
  • R 1 represents an alkyl and/or alkenyl group with a hydrocarbon chain length of 5 to 22 carbon atoms
  • n 2 takes
  • composition may be characterized in that said alkyl polyethylene glycol sulfate with the general formula R 1 -O(-CH 2 -CH 2 -O)n 1 (SO 3 ) n 2 X 1 is the commercially available product EMAL 270D or (SULFANOR TM ) Sulfoethoxylates of fatty alcohols.
  • Sulfoethoxylates of fatty alcohols is available from Norkem, in particular, and can be identified, for example, by ⁇ https://www.norchem.ru/products/ani choire-pav/sulfanor-sulfoetoxilaty-zhirnyh-spirtov-sles-marki-b1-b2-b2k-b3.html>.
  • composition may be characterized in that the mass ratio of Pristine ® 100L/ Pristine ® 100T and EMAL 270D/ (SULFANOR TM ) is (0.00125-0.25):(0.50-25.00).
  • composition may be characterized in that the activity of said nuclease is at least 100 U/g at pH 4.0-7.0.
  • composition may differ in that the activity of the said protease is at least 80 U/g, preferably at least 100 U/g, at pH 4.0-7.0.
  • the composition may be characterized in that said household detergent is selected from laundry detergent, including delicate laundry detergent or baby and child laundry detergent, laundry conditioner, stain remover for pre-treating and washing laundry, laundry gel and laundry conditioner.
  • laundry detergent including delicate laundry detergent or baby and child laundry detergent, laundry conditioner, stain remover for pre-treating and washing laundry, laundry gel and laundry conditioner.
  • the invention relates to a laundry detergent containing 0.50-25.50 wt.% of the composition according to the invention.
  • the laundry detergent may be characterized in that the detergent in question is selected from a delicate laundry detergent and a baby or child laundry detergent.
  • the laundry detergent may be characterized in that the detergent in question is selected from a detergent for membrane fabrics and a detergent for sportswear.
  • the laundry detergent may be characterized in that the detergent in question is a powder laundry detergent.
  • the invention relates to the use of a composition according to the invention for enzymatic degradation of biostains, regulation of the kinetics of nucleic acid-rich sweat crystallisation and neutralisation of unpleasant odours on various fabrics, maintaining long-lasting cleanliness and pleasant fragrance.
  • the use may be characterized in that said fabric is selected from cotton, synthetic, blended, delicate (wool, silk, cashmere, merino, down, feather and their mixtures), membrane.
  • the invention relates to a composition intended for use in laundry detergents and active at a pH of 5.5-10.5 and a water hardness of 0-15 °dH, consisting of:
  • the mass content of component A in said mass ratio may be 0.00125, 0.01, 0.05, 0.1, 0.2 or 0.25 or any value in between.
  • the mass content of component B in said mass ratio may be 0.0005, 0.001, 0.01, 0.1, 0.2 or 0.25 or any value in between.
  • the mass content of component C in the said mass ratio may be 0.5, 1, 5, 10, 15, 20 or 25 or any value in between.
  • the composition may be characterized in that the bionuclease is a nuclease or deoxyribonuclease (DNAase) obtained biotechnologically from microorganisms, in particular fungi or yeast-like fungi.
  • the nuclease can be a substance or a commercially available product with registration number CAS 9003-98-9.
  • the composition may be characterized in that the bioprotease is a protease from the class of serine proteases obtained biotechnologically from microorganisms, in particular bacteria.
  • the protease can be a substance or a commercially available product with the registration number CAS 9014-01-1.
  • the composition may be characterized in that the alkyl and/or alkyl sulfate and/or alkyl polyethylene glycol sulfate may be a C12-C18 monoalkyl sulfate, such as Sulfopon G1218 or Sulfopon G1218 MB, C10-16 alkyl polyethylene glycol sulfate 1-3 EO, such as EMAL 270D or (SULFANOR TM ) Sulfoethoxylates of fatty alcohols, potassium cocoate such as Mackadet 40K or Eurasol KPZ SG or C12-C18 fatty acids sodium salts such as sodium palmitate or another commercially available components.
  • These products specified are commercially available products and are suitable for incorporation into the formulations.
  • the present invention also relates to the use of the composition according to the invention in a household detergent.
  • the household detergent according to the invention may contain 0.50-50.25 wt.% of the composition according to the invention.
  • the household detergent according to the invention may contain 0.50, 1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50 or 50.25 wt.% or any value in between of said compositions according to the invention.
  • the present invention also relates to a laundry agent, a laundry agent for sportswear or a laundry agent for baby's or children's laundry containing 0.50-50.25 wt.% of the composition according to the invention.
  • the present invention also relates to a powder detergent for washing white and coloured laundry containing 0.50-50.25 wt.% of the composition according to the invention.
  • the acceptable excipients may be selected from the following categories of components.
  • the remedy or composition according to the invention may contain, wt.%: nuclease Nuclease or Deoxyribonuclease or DNAse 0.00125-0.25%, protease Protease or Subtilisin 0.00050-0.25%, alkyl and/or alkyl sulphate and/or alkyl polyethylene glycol sulphate with the general formula R 1 -O(-CH 2 -CH 2 -O)n 1 (SO 3 )n 2 X 1 0.5-25.00%.
  • the present invention also relates to the use of a composition according to the invention to remove biostains and regulate the kinetics of nucleic acid enriched sweat crystallisation on various fabrics, including cotton, synthetic, mixed, membrane fabrics, to maintain long lasting cleanliness and whiteness.
  • the present invention also relates to a high performance blend intended for use in household cleaning products, consisting of: 1) the nuclease Pristine ® 100L or Pristine ® 100T; 2) a protease (subtilisin) as part of the enzyme complex Medley Brilliant 300L, and 3) an anionic surfactant selected from Eurasol KPZ SG, EMAL 270D, (SULFANOR TM ) Sulfoethoxylates of fatty alcohols, Sulfopon 1216G, MASCID 2012 or a mixture of two or more of these, wherein the specified ingredients of the blend are taken in the ratio (0.00125-0.25):(0.0005-0.25):(0.5-25) respectively.
  • the mass content of component (1) in said mass ratio may be 0.00125, 0.01, 0.05, 0.1, 0.2 or 0.25 or any value in between said values.
  • the mass content of component (2) in said mass ratio may be 0.0005, 0.001, 0.01, 0.1, 0.2 or 0.25, or any value in between said values.
  • the mass content of component (3) in said mass ratio may be 0.5, 1, 5, 10, 15, 20 or 25 or any values in between said values.
  • composition preferably does not contain other active agents and/or excipients, such as washing active agents and/or acceptable excipients, but may contain them.
  • active agents may represent or represent agents traditionally used in the art and which are known to the skilled person in the art.
  • the addition of said agents to the composition of the complex according to the invention does not invalidate the achievement of the claimed technical results, but can improve them.
  • laundry detergent within the scope of the invention are also household chemicals such as laundry detergent, laundry detergent for sportswear, laundry detergent for baby's or children's laundry, powdered laundry detergent.
  • the acceptable excipients may be selected from the following categories of components.
  • Nonionic surfactants are nonionic surfactants:
  • the invention relates to the use of the composition according to the invention for enzymatic cleavage of biostains, regulation of nucleic acid enriched sweat crystallisation and neutralisation of unpleasant odours on various fabrics, maintaining a long lasting purity and pleasant fragrance.
  • the use may be characterized in that said fabric is selected from cotton, synthetic, blended, delicate (wool, silk, cashmere, merino, down, feather and their mixtures), membrane.
  • component C) appears to be a "game-changer" for combining components A) and B) to obtain beneficial and synergistic technical effects of the claimed composition, as described in paragraph [0007] above.
  • component C appears to be a "game-changer" for combining components A) and B) to obtain beneficial and synergistic technical effects of the claimed composition, as described in paragraph [0007] above.
  • a liquid detergent (formula No. 1), in particular a universal laundry detergent for white and coloured laundry, was prepared within the scope of the invention (Table 1). Table 1. Formulation of the liquid laundry detergent with the claimed composition No.
  • n 1 takes a value from 0 to 10 and denotes the number of polyethylene groups
  • R 1 represents an alkyl and/or alkenyl group with a hydrocarbon chain length of 5 to 22 carbon atoms
  • n 2 takes a value from 0 to 1 and denotes number of sulfate groups
  • X 1 represents alkali and/or alkaline earth metal cation, ammonium, alkylammonium, alkanolammonium, glucoammonium, basic amino acid 2.5-10.00 3 alkyl glucoside with the general formula: R 28 -O-[G]p 3 , wherein R 28 is an alkyl and/or alkenyl group with a hydrocarbon chain length of 4 to 22 carbon atoms, G is
  • Prepared liquid detergent for laundry washing and stain removal provides high efficiency of nucleic acids breakdown in the biostains and reduction of crystallisation of sweat enriched with nucleic acids from various fabrics, in particular cotton, synthetic, membrane, linen, at any hardness of tap water of 0-15 °dH and at any washing temperature from +15 °C to +60 °C. It does not cause colour change and dye washout, retains the product's original appearance, leaves no streaks, is completely rinsed from the surface of fabric, suitable for washing children's laundry and people with sensitive hands, stable in storage for 24 months (observation time).
  • composition according to the invention were investigated in various detergent formulations.
  • a dry detergent (formula No. 2), in particular a powdered detergent for washing coloured laundry and stain removal, was prepared within the scope of the invention (Table 2).
  • Table 2 Formulation of the powdered detergent for washing coloured laundry with the claimed composition No.
  • R 3 is an alkyl and/or alkenyl group with a hydrocarbon chain length of 6 to 22 carbon atoms
  • X 3 is an alkali and/or alkaline earth metal cation, ammonium, alkylammonium, alkanolammonium, glucoammonium 2.5-10.0 3 alkylpolyethylene glycol ether with the general formula: R 29 -O(-CH 2 -CH 2 -O-)n 3 H, wherein n 3 can take values from 2 to 20 and denotes the number of polyethylene glycol groups
  • R 29 is an alkyl and/or alkenyl group with a hydrocarbon chain length of 6 to 22 carbon atoms 2.5-10.0 4 alkyl carboxylate with the general formula R1-O(-CH2-CH2-O)n1 (SO3)n2 X1, wherein n1
  • Prepared powdered detergent for washing coloured linen and stain removal ensures high efficiency of breaking down nucleic acids in the composition of biostains and reduction of crystallisation of sweat enriched with nucleic acids from various fabrics, in particular cotton, synthetic, membrane, linen, at any hardness of tap water 0-5 mg-eq/1 and at any washing temperature from +15°C to +60°C. It does not cause colour change and dye washout, retains the product's original appearance, does not leave streaks, rinses off the surface of fabric, suitable for washing children's linen and people with sensitive hands, stable when stored for 36 months (observation time).
  • composition according to the invention were investigated in various detergent formulations.
  • a dry detergent (formula No. 3), in particular a powdered detergent for washing white laundry and stain removal, was prepared within the scope of the invention (Table 3).
  • Table 3 Formulation of the powdered detergent for washing white laundry with the claimed composition No.
  • R 3 is an alkyl and/or alkenyl group with a hydrocarbon chain length of 6 to 22 carbon atoms
  • X 3 is an alkali and/or alkaline earth metal cation, ammonium, alkylammonium, alkanolammonium, glucoammonium 2.5-10.0 3 alkylpolyethylene glycol ether with the general formula: R 29 -O(-CH 2 -CH 2 -O-)n 3 H, wherein n 3 can take values from 2 to 20 and denotes the number of polyethylene glycol groups
  • R 29 is an alkyl and/or alkenyl group with a hydrocarbon chain length of 6 to 22 carbon atoms 1.0-10.0 4 alkyl carboxylate with the general formula R1-O(-CH2-CH2-O)n1 (SO3)n2 X1, wherein n
  • Prepared powdered detergent for washing white laundry and stain removal provides high efficiency of breaking down nucleic acids in the composition of biostains and reducing the crystallisation of sweat enriched with nucleic acids from various fabrics, in particular cotton, synthetic, membrane, linen, at any hardness of tap water of 0-15 °dH and at any washing temperature from +15°C to +60°C. It does not cause destruction of fabric structure and excessive washout of dye, retains the same appearance of the product, does not leave streaks, rinses off the surface of fabric, suitable for washing baby or children laundry and people with sensitive skin of hands, stable in storage for 36 months (observation time).
  • test methodology is based on the generally recognised recommendations of the European Association A.I.S.E. [A.I.S.E. Laundry Detergent Testing Guidelines Minimum requirements for comparative detergents testing, see https://www.aise.eu/documents/document/20180625164030-laundry_detergent testing_guidelines_v_5_2_june_2018_.pdf, to determine the detergent efficacy with regard to stain removal index (SRI) and the values L,a,b.
  • SRI stain removal index
  • the SRI is calculated using the formula from the ASTM D 4265 Standard guide for evaluating stain removal performance in home laundering [DOI: 10.1520/D4265-14].
  • SRI is a quantitative measure of the removal of dirt and stains in units. The values are based on whiteness L and chromaticity a,b, calculated mathematically using a special formula from ASTM D 4265-14.
  • the method has numerous advantages, in particular, high accuracy, reproducibility, low error rate (less than 5%), takes into account colours of laundry and their change in the process of washing, different types of fabric and is the closest to the real visual perception and balances out the effect of optical dyes, common in detergents for laundry washing.
  • a difference of 2 or more SRI units is effective and statistically significant. In practical experience, increased 1 unit of SRI corresponds to 5% increased detergent efficacy and gives a significant contribution to the formulation.
  • the studied composition in the liquid universal laundry detergent for white and colored laundry has a strong stain-removing effect in respect of complex soiling of protein, pigment and oil, mixed nature based on low-fusible and refractory saturated and/or unsaturated acids and their monoacyl, diacyl and triacylglycerides, nucleic acids, coloured pigments and organic biopolymers, compared to the comparison means, not containing the composition according to the invention.
  • composition with 0.006% bioprotease and 0.005% bionuclease increased SRI by 77.9 units for all stains and in particular for sweat, skin sebum, grease and blood based on nucleic acids, showing synergism between the components and statistically significant result in removing biostains during washing (p ⁇ 0.05).
  • the base sample without the composition of the invention did not show a very high removal of the selected biostains, indicating a lack of efficacy of the surfactants in removing the soiling during laundry (Table 5).
  • composition of a universal liquid detergent for washing coloured and white laundry based on natural aerobically biodegradable surfactants, bioprotease and bionuclease allows effective removal of soiling from surface and hard-to-reach areas of various fabrics, thus reducing the total amount of surfactants in household detergents, maintaining dermatological safety for humans and achieving almost complete removal of biological soiling from the laundry.
  • Removal of fatty grease stains, sweat and sebum crystals, epidermal cell proteins and exfoliated particles provides a hygienic function, prevents excessive sorption of contaminants on fabric surfaces, rapid accumulation of bad smells on garments and reduces the number of washings in the long term.
  • Increased SRI demonstrates high light-reflectivity of the fabric, the appearance of whiteness and a reduction of jaundice on the surface of the garment, indicating crystal-clear laundry without optical brighteners.
  • bionuclease improves the effectiveness of removing food stains (cocoa, grass, fruit) and blood stains without damaging the fabric, and allows to regulate the content of bioprotease in the product, without exceeding the dermatological tolerance of protease in laundry detergents from HERA and ECHA.
  • the reduced content of enzymes, in particular proteases, and surfactants allows for low toxicity, low skin irritation potential on the hands and the possibility of allergic reactions in humans when wearing laundry washed with the product.
  • a laboratory study of the detergent efficacy of the composition in a powdered detergent for washing white laundry with the claimed composition was carried out.
  • a powdered laundry detergent for coloured laundry was used as a base for introducing components, as shown in Table 2.
  • test methodology is based on the generally recognised recommendations of the European Association A.I.S.E. [A.I.S.E. Laundry Detergent Testing Guidelines Minimum requirements for comparative detergents testing, see https://www.aise.eu/documents/document/20180625164030-laundry_detergent_testing_guidelines_v_5_2_june_2018_.pdf, to determine the detergent efficacy with regard to stain removal index (SRI) and the values L,a,b.
  • SRI stain removal index
  • the SRI is calculated using the formula from ASTM D 4265 Standard guide for evaluating stain removal performance in home laundering [DOI: 10.1520/D4265-14].
  • SRI is a quantitative measure of the removal of dirt and stains in units. The values are based on whiteness L and chromaticity a,b, calculated mathematically using a special formula from ASTM D 4265-14.
  • the method has many advantages, in particular, high accuracy, reproducibility, low error (less than 5%), takes into account colours of laundry and their change in the process of washing, different types of fabric and is the closest to the real visual perception and balances out the effect of optical dyes, common in detergent composition for laundry washing.
  • a difference of 2 or more SRI units is effective and statistically significant. In practical experience, increased 1 unit of SRI corresponds to 5% increased detergent efficacy and gives a significant contribution to the formulation.
  • the studied composition in the powdered detergent for washing white laundry has a strong stain-removing effect against complex biological stains based on low-fusible and refractory saturated and/or unsaturated acids and their monoacyl, diacyl and triacylglycerides, nucleic acids, coloured pigments compared to the comparison means, not containing the composition according to the invention.
  • composition with 0.05% bioprotease and 0.005% bionuclease increased the SRI by 4.7 units for the sweat and skin sebum biocontamination based on nucleic acids, which is a manifestation of synergy between the components and statistically significant result in biostain removal during washing (p ⁇ 0.05).
  • the base sample without the composition of the invention showed a low removal of the selected biostain, indicating a lack of efficacy of the surfactants for the removal of contaminants during laundering (Table 7).
  • Table 7 Assessment of the effectiveness of C-P244PDE specific contaminant removal Test specimen Components of the composition SRI score C-P244PDE (in. contamination 32.2) sample No.
  • composition of a powdered detergent for washing white laundry based on natural aerobically biodegradable surfactants, bioprotease and bionuclease enables effective removal of soiling from surface and hard-to-reach areas of various fabrics, thus reducing the total amount of surfactants in household detergents, maintaining dermatological safety for humans and achieving almost complete removal of biological soiling from laundry. Removal of fatty grease stains, sweat and sebum crystals, epidermal cell proteins and exfoliated particles provides a hygienic function, prevents excessive sorption of contaminants on fabric surfaces, rapid accumulation of bad smells on garments and reduces the number of washings in the long term.
  • Increased SRI demonstrates high light-reflectivity of the fabric, the appearance of whiteness and a reduction of jaundice on the surface of the garment, indicating crystal-clear laundry without optical brighteners.
  • the addition of a bionuclease enhances the removal of stains from human excreta without damaging the fabric, and controls the bioprotease content of the product, without exceeding the dermatological tolerance of the protease in HERA and ECHA laundry detergents.
  • the reduced content of enzymes, in particular proteases, and surfactants allows for low toxicity, low skin irritation potential on the hands and the possibility of allergic reactions in humans when wearing laundry washed with the detergent.
  • test methodology is based on the generally recognised recommendations of the European Association A.I.S.E. [A.I.S.E. Laundry Detergent Testing Guidelines Minimum requirements for comparative detergents testing, see https://www.aise.eu/documents/document/20180625164030-laundry_detergent_testing_guidelines_v_5_2_june_2018_.pdf, to determine the detergent efficacy with regard to stain removal index (SRI) and the values L,a,b.
  • SRI stain removal index
  • the SRI is calculated using the formula from ASTM D 4265 Standard guide for evaluating stain removal performance in home laundering [DOI: 10.1520/D4265-14].
  • SRI is a quantitative measure of the removal of dirt and stains in units. The values are based on whiteness L and chromaticity a,b, calculated mathematically using a special formula from ASTM D 4265-14.
  • the method has numerous advantages, in particular, high accuracy, reproducibility, low error (less than 5%), takes into account colours of laundry and their change in the process of washing, different types of fabric and is the closest to the real visual perception and balances out the effect of optical dyes, common in detergents for laundry washing.
  • a difference of 2 or more SRI units is effective and statistically significant. In practical experience, 1 unit of SRI corresponds to 5% detergent efficacy and gives a significant contribution to the formulation.
  • the studied composition in the liquid universal laundry detergent for white and colored laundry has a strong stain-removing effect against complex stains of protein, pigment and oil, mixed nature based on nucleic acids, pigments and protein compounds, compared to the comparison means, not containing the composition according to the invention.
  • composition with 0.006% bioprotease and 0.005% bionuclease increased the SRI index by 4.8 units with regard to protein and nucleic acid based contamination, which is a manifestation of synergy between the components and statistically significant result in the removal of biostains during washing (p ⁇ 0.05).
  • the base sample without the composition of the invention did not show high removal of the selected biostains, indicating insufficient efficacy of the surfactants for the removal of soiling during laundering (Table 9).
  • Table 9 Evaluation of the effectiveness of EMPA 116 biocontamination removal Test specimen Components of the composition SRI score EMPA 116 sample No. 1 base aerobically fast biodegradable 57.7 surfactant SLES 5.25+0.25% sample No.
  • composition of the universal liquid laundry detergent for coloured and white laundry based on natural aerobically biodegradable surfactants, bioprotease and bionuclease allows effective removal of soiling from surface and hard-to-reach areas of various fabrics, thus reducing the total amount of surfactants in household detergents, maintaining dermatological safety for humans and achieving almost complete removal of biological soiling from the laundry. Removal of sweat crystals and sebum, epidermal cell proteins and exfoliated particles provides a hygienic function, prevents excessive sorption of contaminants on fabric surfaces, rapid accumulation of unpleasant odours on garments and reduces the number of washings in the long term.
  • Increased SRI demonstrates high light-reflectivity of the fabric, the appearance of whiteness and a reduction of jaundice on the surface of the garment, indicating crystal-clear laundry without optical brighteners.
  • the addition of a bionuclease enhances the removal of blood stains without damaging the fabric, and controls the bioprotease content of the product, without exceeding the dermatological tolerance of the protease in HERA and ECHA laundry detergents.
  • the reduced content of enzymes, in particular proteases, and surfactants allows for low toxicity, low skin irritation potential on the hands and the possibility of allergic reactions in humans when wearing laundry washed with the product.
  • a laboratory study of the detergent efficacy of the composition in a universal liquid laundry detergent was carried out.
  • test methodology is based on the generally recognised recommendations of the European Association A.I.S.E. [A.I.S.E. Laundry Detergent Testing Guidelines Minimum requirements for comparative detergents testing, see https://www.aise.eu/documents/document/20180625164030-laundry_detergent_testing_guidelines_v_5_2_june_2018_.pdf, to determine the detergent efficacy with regard to the stain removal index (SRI) and the values L,a,b.
  • SRI is calculated using the formula from ASTM D 4265 Standard guide for evaluating stain removal performance in home laundering [DOI: 10.1520/D4265-14].
  • Designation Composition Type EMPA 116 blood/milk/ink mixed protein + pigmented EMPA 117 blood/milk/ink mixed: protein + pigmented WE5DASBWKC blood enzymatic protein CFT CS-44 chocolate drink enzymatic protein CFT CS-07 grass enzymatic protein WFK 20PF vegetable oil enzymatic oil CFT CS-32 skin sebum enzymatic oil CFT CS-406 balsamic sauce enzymatic mannanase EMPA 165 chocolate pudding enzymatic mannanase WE5TPWKC tomato puree enzymatic pectate C-H147 fruit jam enzymatic pectate CFT CS-28 rice starch coloured enzymatic amylase CFT CS-29 tapioca starch coloured enzymatic amylase KC-H097 porridge enzymatic cellulase C-01 mineral oil pigmento-oil WE5FSMWKC mustard pigmento-oil WE5FM2WKC decorative cosmetics
  • Nucleic acid (DNA) based skin sebum stain removal was also evaluated: NZ-117 DNA and PC-H244 DNA (Table 11).
  • the selected hard to remove nucleic acid based biostains allowed both the overall detergent efficacy of the formulation and the efficacy against individual contaminants to be evaluated due to the presence of a bionuclease and bioprotease according to the invention.
  • the test was carried out in a TOM laboratory washing machine at 30° C, a washing time of 20 minutes, spin speed of 120 PRM, a water hardness of 15° dH, and a dosage of 3.33 g/l.
  • SRI was measured at Rem 460 nm. Table 11.
  • SRI is a quantitative measure of the removal of dirt and stains in units. The values are based on whiteness L and chromaticity a,b, calculated mathematically using a special formula from ASTM D 4265-14.
  • the method has numerous advantages, in particular, high accuracy, reproducibility, low error (less than 5%), takes into account colours of laundry and their change in the process of washing, different types of fabric and is the closest to the real visual perception and levels the effect of optical dyes, common in detergents for laundry washing. A difference of 2 or more SRI units is effective and statistically significant. In practical experience, increased 1 unit of SRI corresponds to 5% increased detergent efficacy and gives a significant contribution to the formulation.
  • the studied composition in the liquid universal laundry detergent for white and colored laundry has a strong stain-removing effect in respect of complex soiling of protein, pigment-oil, polysaccharide, lipid, bleach and mixed nature based on low-fusible and refractory saturated and/or unsaturated acids and their mono-, diacyl- and triacylglycerides, nucleic acids, stained pigments and organic biopolymers.
  • the addition of a high-performance bionuclease in combination with a bioprotease together with the selected components of the composition increased the degree of removal of complex nucleic acid (DNA)-based sebum contaminants.
  • the addition of 0.005% bionuclease significantly increased the SRI relative to the base product with respect to biological contamination based on sebum, sweat crystals, bacterial biofilms and nucleic acid-rich skin epidermis.
  • composition with 0.0035% bioprotease and 0.005% bionuclease increased the SRI score by 4.7 units for nucleic acid-based sweat and sebum biocontamination, which is a manifestation of synergism between the components and statistically significant result in biostain removal during washing (p ⁇ 0.05).
  • the base sample without the composition of the invention showed a low removal of selected biostains, indicating the insufficient effectiveness of surfactants in removing these contaminants during laundering (Table 13).
  • Table 13 Assessment of the effectiveness of nucleic acid based stain removal Test specimen Components of the composition SRI score C-P244 DNA NZ-117 DNA total SRI for 2 spots sample No.
  • composition of the universal liquid detergent for washing coloured and white laundry based on the natural aerobically biodegradable surfactants, bioprotease and bionuclease allows effective removal of soiling from surface and hard-to-reach areas of various fabrics, thus reducing the total amount of surfactants in household detergents, maintaining dermatological safety for humans and achieving almost complete removal of all types of biological soiling from laundry.
  • the combination of bionuclease and bioprotease significantly increases the overall washing efficiency against all types of stains, improving the removal of protein, mannan, pectin and cellulose based stains, indicating the role of nucleic acids in fixing contaminants.
  • bionuclease improves the removal efficiency of food stains (cocoa, grass, fruit), blood stains and DNA-rich sebum stains without damaging the fabric and allows the bioprotease content of the product to be adjusted without exceeding the dermatological tolerance of the protease in HERA and ECHA laundry detergents.
  • the test methodology was based on the scientific publication "Deodorizing Ability of Houttuynia cordata Thunb. (Dokudami) for Masking Garlic Odar - Hiromi Ikeura” to determine neutralization of unpleasant odours by the organoleptic method.
  • the sources of unpleasant odours were strong food odours in an alcoholic solution: garlic extract, atlantic herring extract and oil acid extract, which is part of the fatty acids of sweat, sebum. This test evaluates the effectiveness of neutralising unpleasant odours using a composite of cotton and synthetic materials, simulating real washing conditions.
  • the test was carried out on 4.5 cm ⁇ 9.0 cm pieces of cotton fabric which were pre-sprayed with these extracts by 3-fold spraying at a distance of 10-15 cm from the contaminated fabric sample.
  • the tissue samples were then air dried in a horizontal position for 45-60 min.
  • One sample obtained in this way was used as a control initial smell, and the other 4 samples after 20 min soaking in 1 l solution of laundry gel (4.5 g/l) was washed in a Linitest laboratory washing machine by 2 pieces in 100 g of this solution in 2 separate cups in the appropriate washing programme for 20 min at 40 °C with 2 subsequent rinses for 2 min.
  • the samples were air dried horizontally for 2 hours at room temperature after washing.
  • Comparison of odours was carried out organoleptically by comparing the initial smell of the fabric (unwashed) with the smell of fabric samples after washing by placing a fabric sample in the bottom of a 1 litre plastic cup.
  • the odour of the original fabric was evaluated by intensity:
  • composition of a universal liquid detergent for washing coloured and white laundry based on natural aerobically biodegradable surfactants, bioprotease and bionuclease allows effective removal of unpleasant odours from surfaces and hard-to-reach areas of various fabrics, thereby improving the organoleptic profile of the laundry.
  • the combination of bionuclease and bioprotease significantly increases the overall deodorising effectiveness against common unpleasant odours, including sweat, nucleic acid-rich sebum.
  • the washing effectiveness of the composition in a universal liquid laundry detergent was investigated in relation to sweat crystals enriched with nucleic acids and mineral deposits of sweat glands, and hard-to-remove fixable skin sebum.
  • test methodology is based on the study of cotton pillowcase samples before and after washing to determine the removal efficiency of hard-to-remove fixable skin sebum and to assess the kinetics of nucleic acid-rich sweat crystal formation.
  • a group of 10 healthy volunteers took part in the study.
  • participants were given clean white cotton pillowcases to use during the 14 days of the study.
  • participants returned the contaminated pillowcases, evaluated the aroma intensity using the methodology in Example 8, and the appearance of the pillowcases.
  • the images were analyzed to obtain data on the fraction of area from which the fluorescence signal from contamination arrives and the signal intensity. Based on the results, the proportion of contaminated area and the number and size of contaminant particles, in particular sweat crystals and dermal sebum, were estimated using ImageScope software.
  • Test specimen Components of the composition Contaminated surface area, % Average size of sweat crystals, ⁇ m prior after 5 washings prior after 5 washings sample No. 1 base aerobically fast biodegradable 14.65 + 5.73 0.53 + 0.13 * 2.15+0.78 2.15+0.78 surfactant SCS 5.00 + 0.25% sample No.
  • Base sample without the composition according to the invention showed removal of selected biocontaminants due to surfactants, but poorly regulated the crystal formation of sweat enriched with nucleic acids, indicating insufficient effectiveness of surfactants for deep hygienic cleaning in the laundry, the subsequent preservation of bad odours and creating a favourable environment for the growth of microflora on the laundry.
  • the test was carried out in the Novozymes A/S laboratory using the company's own methodology to determine enzyme activity after 4 weeks in a chamber at 30 °C. This test evaluates the preservation of enzyme activity in the base of the finished product using an accelerated temperature stability technique to identify the residual activity over the shelf life and expiry live, and thus the efficacy of the product.
  • composition of universal liquid detergent for washing coloured and white laundry based on natural aerobically biodegradable surfactants, bioprotease and bionuclease retains its activity during product shelf life to achieve high removal of a wide range of human biostains and biocontaminants, regulation of crystal formation kinetics, neutralization of unpleasant odours from surface and hard to reach areas of different fabrics, thereby improving organoleptic profile of the laundry.
  • the bionuclease is stable in the presence of bioprotease due to the selected combination of formulations given in Examples 1-3 and the use of aerobically biodegradable surfactant as a mycelling and stabilising agent.
  • the nuclease is not stable in the presence of the protease in most means, the results presented above show a new technical result achieved by the characteristics of the raw materials and the possibility of combined use to achieve the claimed effects of the composition according to the invention.
  • the effects of the composition according to the invention, in particular bioproteases and bionucleases, on the destruction of biofilms formed by bacteria on the various house surfaces were investigated in the laboratory.
  • the universal liquid household detergent (universal detergent) indicated in Table 17 was used as the base for incorporating the components of the composition. Samples of the liquid detergent with a pH of 7.5-8.0 were prepared under laboratory conditions. Table 17. Formulation of the universal liquid detergent with the claimed composition No.
  • R 1 -CO 2 X 1 wherein R 1 is an alkyl and/or alkenyl group with a hydrocarbon chain length of 5 to 21 carbon atoms and X 1 is an alkali and/or alkaline earth metal cation, ammonium, alkylammonium, alkanolammonium, glucoammonium, basic amino acid 1-15 3 alkyl glucoside with the general formula: R 28 -O-[G]p 3 , where R 28 is an alkyl and/or alkenyl 2-15 group with a hydrocarbon chain length from 4 to 22 carbon atoms, G is a saccharide fragment containing 5 or 6 carbon atoms, p 3 can take values from 1 to 4 4 bionuclease 0.001-0.25 5 bioprotease in the form of subtilisin 0.001-0.25 6 complexifier 0.1-0.8 7 cotton extract 0.05-0.
  • Microbial strains widely represented on various surfaces in the home were selected [ Lax S., Smith D.P., Hampton-Marcell J. et al Longitudinal analysis of microbial interaction between humans and the indoor environment // Science, 2014, 345(6200): 1048-1052 ]: conditionally pathogenic and pathogenic microorganisms Staphylococcus aureus ATCC ® 29213, Escherichia coli ATCC ® 25922, Klebsiella pneumoniae (Institute for Medical Microbiology, Giessen, Germany), Pseudomonas aeruginosa ATCC ® 27853, and representatives of the normoflora Staphylococcus epidermidis ATCC ® 14990 and Micrococcus luteus (clinical isolate).
  • the components of the composition were diluted separately and together in a universal liquid detergent to a concentration of 4 wt.%.
  • the universal detergent without the components of the composition was used as a control.
  • the method of analysis is based on the assessment of the antibiofilm activity of compounds and the biofilm-destroying activity of compounds.
  • the first activity is related to the prevention of biofilm formation on surfaces, while the second activity is related to the destruction of already formed stable biofilm on surfaces for elimination and cleaning of surfaces.
  • a special broth was used as a nutrient medium: tryptone 1.0%; yeast extract 0.5%; sodium chloride 0.5%; pH 8.0 [Kayumov et al., 2015], which was prepared from dry components.
  • the medium was sterilized by autoclaving at 121°C for 20 minutes.
  • Agarized medium contained additional 1.5% agar. Glucose was then added to 1% w/v.
  • Microbial biofilms were formed by growing bacteria for two days on BM broth medium containing glucose (1% w/v) in adhesive plates, the culture liquid was removed and test compounds at concentrations of 4, 1, 0.25, 0.06% in a universal liquid detergent were added. After incubation at room temperature for 15 min, the liquid was removed and the wells were washed with PBS, then dried overnight. The wells were then incubated with 100 ⁇ l of a 0.5% solution of crystal violet (Sigma) dissolved in 96% ethanol, followed by a 20 minute incubation period. The plate was then washed with PBS 3-4 times after removing the crystal violet solution from the wells.
  • the parameters for the overall evaluation of the effect of the components of the composition on microbial biofilms were the minimum biofilm suppressing compound concentration (%) and % of the residual microbial biofilm after 15 minutes of exposure to the compounds in the base of said universal liquid detergent, determined by microbiologists according to the results of a laboratory study.
  • the indicator of the residual biofilm of microorganisms makes it possible to assess the effect on the house bacteria and to draw conclusions about the inherent effect of the components of the composition on the various groups of microflora in the house.
  • the composition of the invention has a high antibiofilm activity against various bacteria.
  • Bioprotease and bionuclease have different activity against biofilms of bacteria due to chemical composition of biofilms and predominance of one or another component. It is known that in biofilms of Staphylococcus aureus, Staphylococcus epidermidis and Escherichia coli protein components dominate, while in biofilms of Klebsiella pneumoniae and Pseudomonas aeruginosa - DNA.
  • the highest antibiofilm activity is achieved by combining a bioprotease and a bionuclease in the base of a universal detergent containing alkyl and/or alkyl polyethylene glycol sulphate with the general formula R 1 -O(-CH 2 -CH 2 -O)n 1 (SO 3 ) n 2 X 1 .
  • a universal detergent containing alkyl and/or alkyl polyethylene glycol sulphate with the general formula R 1 -O(-CH 2 -CH 2 -O)n 1 (SO 3 ) n 2 X 1 .
  • the components of the composition have low antibiofilm activity against Staphylococcus epidermidis and only at high concentrations, which makes it possible to target the microflora of house surfaces and retain favourable micro-organisms for dermatological tolerance and the health of house residents.
  • biofilm mass of E. coli, S. aureus, P. aeruginosa and K. pneumoniae was observed after 15 minutes of exposure.
  • Individually components are able to destroy biofilms of conditionally pathogenic microorganisms, but the combination of components of the composition allows to achieve a synergistic antibiofilm effect with preservation of growth of S. epidermidis normoflora (Table 19).
  • Bioprotease allows to destroy biofilms saturated with protein components, while the bionuclease is effective against biofilms of P. aeruginosa and K . pneumoniae, saturated with DNA, which are reservoirs for the growth of microorganisms with resistance to antibacterial agents.
  • the combination of bioprotease and bionuclease (1:1 ratio) can reduce the residual biofilm mass of microorganisms from 20% to 45% during 15 minutes of exposure.
  • the microflora of the home is a harmonious system, so it is important to maintain a balance of micro-organisms both on the skin and on surfaces.
  • Staphylococcus aureus together with other conditionally pathogenic microorganisms such as Escherichia coli, can form stable biofilms from the exopolysaccharide matrix and contribute to various dermatological diseases, including atopic dermatitis [E. Scott, S. Bloomfield. The survival and transfer of microbial contamination via cloths, hands and utensils. J. Appl. Bacteriol. 68 (1990) 271-278 ].
  • the components of the composition according to the invention specifically influence the formation of stable protective biofilms of microorganisms, to which resistance quickly develops, but do not have a negative effect on the normoflora, which allows to provide directed protection against the development of conditionally pathogenic representatives and to maintain a harmonious biodiversity of the house microflora.
  • the reduction of biofilms of conditionally pathogenic and pathogenic strains will help to shift biodiversity towards beneficial resident skin microflora and improve the dermatological condition of the skin in general when in contact with various surfaces.
  • combining the components into a single complex makes it possible to achieve a synergistic effect against conditionally pathogenic microorganisms ( S. aureus, E. coli, P. aeruginosa, K.
  • the claimed composition has a high antibiofilm effect against conditionally pathogenic transient strains that are a causal factor in the development of dermatological and systemic diseases and representatives of the transient microflora at home. Because it preserves the normal microflora of the surfaces of the home at the place of application, it is possible to use it as part of household cleaning products on a regular basis.

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US20200190436A1 (en) * 2017-04-06 2020-06-18 Novozymes A/S Cleaning compositions and uses thereof
US20220333039A1 (en) * 2019-12-23 2022-10-20 The Procter & Gamble Company Compositions comprising enzymes

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EP3898919A1 (de) * 2018-12-21 2021-10-27 Novozymes A/S Waschmittelbeutel mit metalloproteasen

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20200190436A1 (en) * 2017-04-06 2020-06-18 Novozymes A/S Cleaning compositions and uses thereof
US20220333039A1 (en) * 2019-12-23 2022-10-20 The Procter & Gamble Company Compositions comprising enzymes

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CAS , no. 9014-01-1
E. SCOTTS. BLOOMFIELD: "The survival and transfer of microbial contamination via cloths, hands and utensils", J. APPL. BACTERIOL., vol. 68, 1990, pages 271 - 278, XP008039286
LAX S.SMITH D.P.HAMPTON-MARCELL J. ET AL.: "Longitudinal analysis of microbial interaction between humans and the indoor environment", SCIENCE, vol. 345, no. 6200, 2014, pages 1048 - 1052

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