EP4144349A4 - Rna-interferenz-induzierende nukleinsäure, die 8-oxoguanin umfasst, modifizierte nukleinsäure, die an microrna bindet und 8-oxoguanin umfasst, und verwendungen davon - Google Patents

Rna-interferenz-induzierende nukleinsäure, die 8-oxoguanin umfasst, modifizierte nukleinsäure, die an microrna bindet und 8-oxoguanin umfasst, und verwendungen davon Download PDF

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Publication number
EP4144349A4
EP4144349A4 EP20892292.2A EP20892292A EP4144349A4 EP 4144349 A4 EP4144349 A4 EP 4144349A4 EP 20892292 A EP20892292 A EP 20892292A EP 4144349 A4 EP4144349 A4 EP 4144349A4
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EP
European Patent Office
Prior art keywords
oxoguanine
nucleic acid
microrna
rna interference
inducing
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Pending
Application number
EP20892292.2A
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English (en)
French (fr)
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EP4144349A1 (de
Inventor
Sung Wook Chi
Hee Young Seok
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Korea University Research and Business Foundation
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Korea University Research and Business Foundation
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Publication of EP4144349A1 publication Critical patent/EP4144349A1/de
Publication of EP4144349A4 publication Critical patent/EP4144349A4/de
Pending legal-status Critical Current

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A61K31/19—Carboxylic acids, e.g. valproic acid
    • A61K31/195—Carboxylic acids, e.g. valproic acid having an amino group
    • A61K31/197—Carboxylic acids, e.g. valproic acid having an amino group the amino and the carboxyl groups being attached to the same acyclic carbon chain, e.g. gamma-aminobutyric acid [GABA], beta-alanine, epsilon-aminocaproic acid or pantothenic acid
    • A61K31/198—Alpha-amino acids, e.g. alanine or edetic acid [EDTA]
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    • A01K2227/10—Mammal
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EP20892292.2A 2019-11-28 2020-11-30 Rna-interferenz-induzierende nukleinsäure, die 8-oxoguanin umfasst, modifizierte nukleinsäure, die an microrna bindet und 8-oxoguanin umfasst, und verwendungen davon Pending EP4144349A4 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20190156147 2019-11-28
PCT/KR2020/017341 WO2021107747A1 (ko) 2019-11-28 2020-11-30 8-옥소구아닌을 포함하는 rna 간섭 유도 핵산, 8-옥소구아닌을 포함하는 마이크로rna와 결합하는 변형 핵산 및 이들의 이용

Publications (2)

Publication Number Publication Date
EP4144349A1 EP4144349A1 (de) 2023-03-08
EP4144349A4 true EP4144349A4 (de) 2024-04-24

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Application Number Title Priority Date Filing Date
EP20892292.2A Pending EP4144349A4 (de) 2019-11-28 2020-11-30 Rna-interferenz-induzierende nukleinsäure, die 8-oxoguanin umfasst, modifizierte nukleinsäure, die an microrna bindet und 8-oxoguanin umfasst, und verwendungen davon

Country Status (6)

Country Link
US (1) US20230227820A1 (de)
EP (1) EP4144349A4 (de)
JP (1) JP2023513657A (de)
KR (1) KR20210066758A (de)
CN (1) CN115052590A (de)
WO (1) WO2021107747A1 (de)

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WO2010111290A1 (en) * 2009-03-23 2010-09-30 University Of Utah Research Foundation Methods and compositions related to modified guanine bases for controlling off-target effects in rna interference
WO2014108529A1 (en) * 2013-01-14 2014-07-17 Sarepta Therapeutics, Inc. Inhibitory oligonucleotides and their use in therapy
CN104878117A (zh) * 2015-06-24 2015-09-02 青岛大学 一种氧化修饰的微小rna筛选方法
CN104940230A (zh) * 2015-06-24 2015-09-30 青岛大学 一种通过自由基攻击微小rna造成rna氧化损伤的方法
WO2018209288A1 (en) * 2017-05-12 2018-11-15 Massachusetts Institute Of Technology Argonaute protein-double stranded rna complexes and uses related thereto

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KR101481007B1 (ko) 2012-07-19 2015-01-14 전남대학교산학협력단 심장 비대 억제 조성물 및 이를 포함하는 심장비대증 치료제
KR102129852B1 (ko) * 2016-10-11 2020-07-24 주식회사 인코드젠 Rna 간섭 유도 핵산 및 이를 포함하는 hpv 감염 유발 암의 치료용 약학 조성물
KR102012943B1 (ko) * 2018-05-31 2019-08-21 고려대학교 산학협력단 마이크로rna의 비정규 표적을 억제하는 rna 간섭 유도 핵산 및 그 용도

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WO2010111290A1 (en) * 2009-03-23 2010-09-30 University Of Utah Research Foundation Methods and compositions related to modified guanine bases for controlling off-target effects in rna interference
WO2014108529A1 (en) * 2013-01-14 2014-07-17 Sarepta Therapeutics, Inc. Inhibitory oligonucleotides and their use in therapy
CN104878117A (zh) * 2015-06-24 2015-09-02 青岛大学 一种氧化修饰的微小rna筛选方法
CN104940230A (zh) * 2015-06-24 2015-09-30 青岛大学 一种通过自由基攻击微小rna造成rna氧化损伤的方法
WO2018209288A1 (en) * 2017-05-12 2018-11-15 Massachusetts Institute Of Technology Argonaute protein-double stranded rna complexes and uses related thereto

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CHOI YU J. ET AL: "Biophysical properties, thermal stability and functional impact of 8-oxo-7,8-dihydroguanine on oligonucleotides of RNA-a study of duplex, hairpins and the aptamer for preQ 1 as models", NUCLEIC ACIDS RESEARCH, 5 October 2016 (2016-10-05), GB, pages gkw885, XP093122842, ISSN: 0305-1048, Retrieved from the Internet <URL:https://watermark.silverchair.com/gkw885.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAA2EwggNdBgkqhkiG9w0BBwagggNOMIIDSgIBADCCA0MGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQM13PwgQH12Df4GFglAgEQgIIDFLM3-wSH7xx5yTEcJ_49tkXj8KXiRabp7bKHTchacDiQzZPGiWSXJsk_iRChF4pWGx8GSB5arkm2lPsb9nIElyZkFzIge> DOI: 10.1093/nar/gkw885 *
HAYDEN PEACOCK ET AL: "Chemical Modification of siRNA Bases To Probe and Enhance RNA Interference", THE JOURNAL OF ORGANIC CHEMISTRY, vol. 76, no. 18, 16 September 2011 (2011-09-16), pages 7295 - 7300, XP055087444, ISSN: 0022-3263, DOI: 10.1021/jo2012225 *
HENRIK E POULSEN ET AL: "RNA modifications by oxidation: A novel disease mechanism?", FREE RADICAL BIOLOGY & MEDICINE, ELSEVIER INC, US, vol. 52, no. 8, 18 January 2012 (2012-01-18), pages 1353 - 1361, XP028475217, ISSN: 0891-5849, [retrieved on 20120128], DOI: 10.1016/J.FREERADBIOMED.2012.01.009 *
KANNAN ARUNKUMAR ET AL: "8-Oxoguanosine Switches Modulate the Activity of Alkylated siRNAs by Controlling Steric Effects in the Major versus Minor Grooves", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 133, no. 16, 31 March 2011 (2011-03-31), pages 6343 - 6351, XP093115266, ISSN: 0002-7863, DOI: 10.1021/ja2003878 *
MARGIT MUTSO ET AL: "RNA Interference-Guided Targeting of Hepatitis C Virus Replication with Antisense Locked Nucleic Acid-Based Oligonucleotides Containing 8-oxo-dG Modifications", PLOS ONE, vol. 10, no. 6, 3 June 2015 (2015-06-03), pages e0128686, XP055555138, DOI: 10.1371/journal.pone.0128686 *
See also references of WO2021107747A1 *
SIMMS CARRIE L ET AL: "Quality control of chemically damaged RNA", CMLS CELLULAR AND MOLECULAR LIFE SCIENCES, BIRKHAUSER VERLAG, HEIDELBERG, DE, vol. 73, no. 19, 7 May 2016 (2016-05-07), pages 3639 - 3653, XP036040429, ISSN: 1420-682X, [retrieved on 20160507], DOI: 10.1007/S00018-016-2261-7 *
ZHENG GENHUA ET AL: "Inhibiting miRNA in Caenorhabditis elegans using a potent and selective antisense reagent", SILENCE, BIOMED CENTRAL, vol. 1, no. 1, 1 April 2010 (2010-04-01), pages 9, XP021084877, ISSN: 1758-907X, DOI: 10.1186/1758-907X-1-9 *

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KR20210066758A (ko) 2021-06-07
JP2023513657A (ja) 2023-04-03
CN115052590A (zh) 2022-09-13
WO2021107747A1 (ko) 2021-06-03
EP4144349A1 (de) 2023-03-08
US20230227820A1 (en) 2023-07-20

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