EP4041860A1 - Dispositif et procédé d'exposition de cellules à un aérosol - Google Patents
Dispositif et procédé d'exposition de cellules à un aérosolInfo
- Publication number
- EP4041860A1 EP4041860A1 EP20797761.2A EP20797761A EP4041860A1 EP 4041860 A1 EP4041860 A1 EP 4041860A1 EP 20797761 A EP20797761 A EP 20797761A EP 4041860 A1 EP4041860 A1 EP 4041860A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- atmosphere
- exposure
- study
- duct
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 239000000443 aerosol Substances 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 24
- 238000004113 cell culture Methods 0.000 claims abstract description 42
- 230000008021 deposition Effects 0.000 claims abstract description 34
- 230000000694 effects Effects 0.000 claims abstract description 12
- 238000005192 partition Methods 0.000 claims abstract description 5
- 238000007599 discharging Methods 0.000 claims abstract description 3
- 239000007788 liquid Substances 0.000 claims description 8
- 239000001963 growth medium Substances 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 238000002347 injection Methods 0.000 claims description 5
- 239000007924 injection Substances 0.000 claims description 5
- 230000001413 cellular effect Effects 0.000 claims description 4
- 238000009630 liquid culture Methods 0.000 claims description 4
- 239000003153 chemical reaction reagent Substances 0.000 claims description 3
- 210000004072 lung Anatomy 0.000 description 6
- 230000008569 process Effects 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 239000002245 particle Substances 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 238000009833 condensation Methods 0.000 description 2
- 230000005494 condensation Effects 0.000 description 2
- 239000003571 electronic cigarette Substances 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 210000005265 lung cell Anatomy 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 230000003278 mimic effect Effects 0.000 description 2
- 239000006199 nebulizer Substances 0.000 description 2
- 229920000139 polyethylene terephthalate Polymers 0.000 description 2
- 239000005020 polyethylene terephthalate Substances 0.000 description 2
- 230000000241 respiratory effect Effects 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- 239000000779 smoke Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- 231100000027 toxicology Toxicity 0.000 description 2
- -1 vapor Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 230000001174 ascending effect Effects 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000036755 cellular response Effects 0.000 description 1
- 230000004637 cellular stress Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 230000000391 smoking effect Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000002110 toxicologic effect Effects 0.000 description 1
- 231100000041 toxicology testing Toxicity 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
- 238000009834 vaporization Methods 0.000 description 1
- 230000008016 vaporization Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/12—Well or multiwell plates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/24—Heat exchange systems, e.g. heat jackets or outer envelopes inside the vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
Definitions
- TITLE DEVICE AND METHOD FOR EXPOSING CELLS TO A
- the invention relates to a device and method for exposing cell cultures to an atmosphere such as an aerosol for toxicological studies.
- Cellular exposure devices to a gas or an aerosol have been proposed to study in vitro the toxic or therapeutic effects associated with the inhalation of substances through the respiratory tract.
- To reproduce the characteristics of lung tissue cell cultures are adhered to a permeable support maintained in liquid culture medium, so as to reproduce the air / liquid interface of lung cells. Exposure of cells is achieved by diluting a test substance in a gas, vapor, or aerosol.
- This atmosphere to be studied is then injected into a cell exposure device comprising an atmosphere inlet connected to a network of conduits supplying a plurality of cell culture chambers.
- the different cell samples thus receive the same atmosphere injected into the device, and these samples are then used to measure a cellular response to the atmosphere tested.
- Vitrocell Systems GmbH and Cultex Laboratories GmbH market these devices.
- the American patent application No. US 2018/0171280 A1 discloses a cell exposure device comprising an aerosol discharge upper part comprising an aerosol inlet dividing into a network of vertical conduits adapting to a plurality of exposure chambers of a cell culture plate.
- this document suggests creating a temperature gradient in the ducts to ensure the deposition on the cells of all of the aerosol pushed through the ducts.
- the aim of the present invention is to provide a cellular exposure device making it possible to approximate the natural conditions of exposure of lung tissue to an atmosphere such as an aerosol or a gas.
- a second aim of the invention is to study the toxicological or therapeutic effect of an atmosphere as a function of a time of exposure of a cell culture to said atmosphere, as well as to observe its effects on cell cultures. medium and long term.
- the invention provides a device for cell exposure to an atmosphere comprising an upper study atmosphere discharge part and a lower part comprising a cell culture plate provided with a plurality of exposure chambers, said upper part being supplied by a study atmosphere and comprising a network of conduits conveying said atmosphere to the exposure chambers, characterized in that:
- the upper part has at least one pair of conduits, an inlet conduit and an atmosphere outlet conduit connected to a deposit nozzle,
- said nozzle is connected in a sealed manner to one of the exposure chambers of the lower part, and comprises a partition separating an atmosphere inlet sub-duct from an atmosphere outlet sub-duct of said exposure chamber, and wherein an atmosphere injected to said exposure chamber is discharged through the outlet sub-duct of the deposition nozzle by suction, in a controlled time and by creating a passing flow of atmosphere.
- the device of the invention makes it possible to control the exposure time of cultures to an atmosphere by programming the evacuation of the atmosphere from the culture chamber. A passing flow of atmosphere is also created on the surface of cell cultures to mimic the process of the respiratory cycle.
- the entry of atmosphere is controlled by opening a solenoid valve connected to the inlet conduit, and the discharge of atmosphere from the exposure chamber is controlled by a proportional solenoid valve connected. to the outlet duct.
- a solenoid valve connected to the inlet conduit
- a proportional solenoid valve connected to the outlet duct.
- the upper discharge portion may include all or some of the following features:
- the at least one pair of atmosphere inlet and outlet conduits is connected to a plurality of deposition nozzles, and preferably to three deposition nozzles;
- the inlet duct comprises successive outlets for supplying a plurality of nozzles, and an internal structure gradually reducing an internal section of the inlet duct as a flow of atmosphere is divided into its interior to supply said successive outlets .
- At least one pair of the atmosphere inlet and outlet ducts is arranged in a horizontal axis, and is connected to S at least one deposition nozzle by means of an inlet pipe and a outlet pipe arranged in a substantially vertical axis, said pipes being optionally connected, respectively, to the deposition nozzle by a fitting.
- the device comprises a plurality of pairs of aerosol inlet and outlet conduits, each inlet conduit allowing independent power supply of a study atmosphere.
- the incubator also includes:
- -a robotic arm comprising interchangeable gripping tools and allowing to manipulate the culture plates of the lower cell exposure part
- a culture step in which samples of cells are cultured in a cell culture plate comprising a plurality of exposure chambers, each exposure chamber being provided with a well comprising liquid culture medium and a sample cells supported by an insert, said insert being introduced into the well so that the cells are located at the air / liquid interface;
- the method of the invention may include all or some of the following characteristics:
- a time of exposure of cells to said study atmosphere is controlled by an output rate of the study atmosphere injected into the exposure chamber.
- the atmosphere discharge device is disconnected from the exposure chambers and a post-exposure culture step is carried out.
- FIG. 1 A simplified front perspective view of the upper atmosphere discharge portion and the lower portion of the device of the invention according to one embodiment.
- FIG. 4 A schematic view of a longitudinal section of a deposition nozzle and insert, exploded, according to one embodiment of the invention.
- FIG. 6 A perspective view of a deposition nozzle according to one embodiment of the invention.
- FIG. 7 A schematic example of an incubator according to one embodiment of the invention.
- FIG. 8 A schematic example showing the inside of the incubator.
- FIG. 9 A diagram of the steps in the process of the invention.
- the present invention provides an exposure device making it possible to control the exposure time of cells to a study atmosphere, as well as reproducing the natural conditions of exposure of lung cells during inhalation of an atmosphere such as a gas or a vapor.
- the device of the invention makes it possible to study the effects of the controlled passage of an atmosphere on cell tissues cultured at the liquid / air interface.
- atmosphere is meant a gas mixture (i.e. air) having a specific chemical composition and which may include liquid or solid particles in suspension.
- the study atmosphere is, for example, an aerosol, vapor, smoke, gas or a suspension of particles in the air.
- the deposition nozzle connects in a sealed manner to one of the exposure chambers of the lower part, and comprises a partition separating an atmosphere inlet sub-duct from an atmosphere outlet sub-duct .
- the injection of the atmosphere and its evacuation by suction can be adjusted so as to create a flow of passage of atmosphere on the inserts of the cell cultures present in the exposure chambers.
- This flow of passage prevents the atmosphere from stagnating on cells, so as to mimic the natural conditions of inhaling air or vapor on a person's lung tissue.
- the device helps prevent condensation of droplets on cell cultures.
- a pair of conduits comprising an atmosphere inlet conduit and an atmosphere outlet conduit is understood, within the meaning of the present invention as a pair of inlet conduits. and exit of atmosphere.
- injection is used to describe the introduction of an atmosphere into the inlet duct, and into the exposure chamber, regardless of the mechanism or method used to effect said introduction.
- the atmosphere inlet pipe and the atmosphere outlet pipe are provided, respectively, with a solenoid valve opening or closing the access and the outlet of the atmosphere. 'atmosphere.
- the outlet duct is provided with a proportional solenoid valve making it possible to adjust the flow rate of the atmosphere passing through.
- the exposure time of the cells can thus be easily controlled by programming the opening and closing of the solenoid valves, and in particular the degree of opening of the proportional solenoid valve connected to the outlet duct.
- the proportional solenoid valve of the outlet duct when an atmosphere is injected into the exposure chamber, is in a partially open configuration to avoid a pressure differential that can take off the cells during the evacuation of the cell. the atmosphere injected into the exposure chamber.
- FIGS 1 and 2 illustrate schematically and in a simplified manner an embodiment of the upper part 100 for discharging atmosphere and the lower part 200 for cell culture.
- the lower culture part comprises a support 210 adapted to receive a cell culture plate comprising a plurality of wells for cultivating individual samples of cells on membrane inserts for cell culture adapted to said wells.
- the inserts are, for example, inserts available commercially and marketed under the registered trademark Corning Transwell R comprising a polyethylene terephthalate (PET) membrane.
- PET polyethylene terephthalate
- the upper part 100 comprises three pairs of inlet 120 and outlet 130 atmosphere conduits, each pair of conduits being connected to one or more deposit nozzles 150 by means of pipes 141 and a connector 145.
- each inlet duct is arranged in a horizontal axis and supplies three deposition nozzles with the same atmosphere.
- the inlet duct 120 is provided with an internal structure 121 gradually reducing the internal section of the duct to supply the three nozzles with the same pressure.
- FIG. 3 shows an exemplary embodiment of the internal structure 121 in which an internal diameter of the duct gradually decreases in the direction of arrival of the flow of atmosphere and forms three segments, each supplying an outlet S1, S2, S3 of atmosphere. connected to a deposit nozzle.
- a segment of pipe of constant section is left before supplying an outlet.
- the deposition nozzles 150 are supported by a movable base 110 of the upper part superimposing said nozzles on the wells of the cell culture plate of the lower part 200.
- the deposition nozzles 150 are positioned on the cell culture wells, forming a sealed connection between the tube-inserts of the wells and said nozzles. This sealed connection is for example ensured by means of an O-ring.
- the movable base 110 comprises two plates 111, 11 T horizontal, parallel and provided with orifices 115 for positioning and receiving the deposition nozzles.
- the nozzles deposition are illustrated in the process of insertion and in FIG. 2 the nozzles 150 are illustrated inserted into their orifices 115 for positioning.
- Figure 4 shows a schematic view of a longitudinal section of the deposition nozzle and an insert.
- the deposition nozzle 150 has a general "Y" structure having in an upper part an inlet port 152, and an outlet port 153 for connecting said deposition nozzle to the inlet 120 and outlet 130 conduits respectively.
- An internal septum 155 of the nozzle divides an inlet flow of atmosphere, descending over a cell culture insert 250, from an exhaust flow of atmosphere ascending through said deposition nozzle 150.
- the deposition nozzle is connected to the inlet pipe and to the outlet pipe by means of deposition pipes and fittings 145.
- the deposition nozzle is designed as a pipe having an internal partition and being connected directly to the aerosol inlet and outlet duct.
- the deposition nozzle comprises a cylindrical lower part comprising a lower groove 159 for receiving an O-ring, and a collar 151 making it possible to block the insertion of the nozzle with an upper part of the nozzle. insert 250.
- nozzle collar 151 locks against an upper ring 259 of insert 250 shown in Figure 5.
- the collar 151 has a hexagonal profile making it possible to immobilize the deposition nozzle against an internal wall of the ring 259 of the insert 250.
- the invention proposes to introduce the deposition nozzle 150 into the insert 250 to position it as close as possible to the cells cultured at the bottom of the insert (Fig. 5).
- a lower end of the deposition nozzle is located 7 mm ⁇ 1 mm from the cells when the nozzle is inserted into the insert.
- the dimensions of the cylindrical part of the deposition nozzle are therefore adapted to the dimensions of the insert in order to allow this positioning.
- a second object of the invention is to provide an exposure device allowing prolonged culture of cell cultures under controlled conditions following their exposure to a study atmosphere.
- the cell exposure device comprises an incubator (Fig. 7) in which the upper atmosphere discharge part and the lower cell culture part are integrated.
- the incubator 300 makes it possible to control atmospheric conditions in which cells are cultured following their exposure to a study atmosphere such as an aerosol generated by the vaporization of an electronic cigarette liquid, smoke, etc.
- the device incubator includes:
- At least one airlock 310 for entering and / or leaving equipment and reagents making it possible to maintain the atmospheric conditions configured during the entry and exit of said elements.
- the device of the invention makes it possible to maintain the cells under controlled conditions for a total duration of exposure and of the desired cell culture.
- the risks of microbial contamination and of cellular stress associated with the handling of cultures following their exposure to an atmosphere are advantageously reduced.
- the culture chambers will be exposed to the study atmosphere injected through the upper part of the device. Once the exposure is complete, the top is disconnected and the exposure chambers are in direct contact with the controlled culture atmosphere inside the incubator.
- part of the exposure chambers are not connected by deposition nozzles during the step of exposure to the study atmosphere, these “control” exposure chambers are therefore in direct and constant contact with the culture atmosphere prevailing inside the incubator. Thanks to these controls, the effect of the culture atmosphere on the response of the cells can be advantageously taken into account.
- Figure 7 shows a schematic view of the incubator 300 of the device of the invention.
- the upper discharge part 100 and the lower cell exposure part 200 are integrated in a sealed enclosure supported by feet 312 and comprising a lateral airlock 310 for the entry and exit of material, in particular the plates of. cell culture, in se rts and culture medium.
- a front wall of the enclosure is advantageously made of a transparent material making it possible to observe the interior of the enclosure and also comprises a pair of gloves making it possible to carry out manipulations inside the enclosure without losing the atmosphere confined to its interior.
- FIG. 8 illustrates in a very simplified manner the interior of the enclosure of the incubator.
- the interior of the enclosure is configured to receive two lower parts 200, 200 'of cell culture and two upper atmosphere discharge parts (pipes 141 not visible).
- each lower part 200, 200 'of cell culture is installed on a heating block 340 and between a pair of vertical rails 315 serving to support the part.
- an elevator 350 and a motor provide automated movement of the upper part.
- the enclosure of the incubator also includes sealed inlets (not shown) to supply an atmosphere to the aerosol inlet conduits 120 of the device, as well as to evacuate the atmosphere from the outlet conduits 130.
- enclosure can also be fitted with one or more electrical outlets, temperature, pressure and / or humidity sensors, lamps, air filters, additional doors allowing entry of bulky materials or equipment , etc.
- the roof of the enclosure follows an inclined plane so as to promote the flow of condensation inside the enclosure and to collect and discharge this water by means of a water collection channel.
- the cell exposure device includes a robotic arm 320 within the enclosure of the incubator.
- This arm automates the preparation of culture plates and features interchangeable gripping tools for handling culture plates, placing in se rts in wells, and adding culture medium.
- the robotic arm uses plate carriers 321 to move heat blocks and culture plates.
- the invention also relates to a method of studying the effects of exposure of cell cultures to an aerosol.
- the method 20 of the invention comprises the following steps shown diagrammatically in Figure 9:
- a culture step 21 in which samples of cells are cultivated in a cell culture plate comprising a plurality of exposure chambers, each exposure chamber being provided with a well comprising liquid culture medium and a sample of cells supported by an insert, said insert being introduced into said well so that the cells are located at the liquid / air interface;
- an exposure step 22 in which an aerosol is injected into the plurality of exposure chambers by means of an aerosol discharge device comprising nozzles sealingly connecting to said plurality of exposure chambers, and in which an aerosol passage flow is created on the cells by a concomitant exit of said injected aerosol.
- the method of the invention is advantageous in that it proposes to expose the cells to a passing flow of aerosol approximating the natural conditions of inhalation / exhalation.
- the method of the invention is particular in that it proposes to expose only part of the plurality of exposure chambers present in a single culture plate. Exposure chambers, or unexposed wells, can be used as controls. Advantageously, these controls will have followed a manipulation almost identical to the exposed samples, thus improving the precision of the results and facilitating the organization of the tests.
- the method of the invention proposes to control the exposure time of the cells by configuring an output rate of the atmosphere injected into the exposure chambers.
- a third post-exposure culture step 23 is then carried out for a given time t, and at the end of which the cell culture samples will be studied to assess their response to the study atmosphere.
- the method of the invention proposes to carry out all the steps of culture 21, exposure 22, and post-exposure culture 23 in a single confined environment and in which the parameters of a culture atmosphere such as temperature, relative humidity and air composition. Carrying out all three steps in a single environment or location minimizes handling of cells and prolongs their life capacity, as well as observing the effect of the culture atmosphere.
- the device and method of the invention are particularly useful in studies of the toxicology of electronic cigarette liquids.
- a vaping robot is connected to the device of the invention, supplying with a single vapor, or different vapors, the atmosphere inlet conduits of the device.
- the device is also compatible with a smoking robot, and other steam or gas generating devices, as well as devices allowing the injection of an atmosphere of particles suspended in the air (nebulizer type).
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Sustainable Development (AREA)
- Immunology (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- Toxicology (AREA)
- Urology & Nephrology (AREA)
- General Physics & Mathematics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Thermal Sciences (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Pathology (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
Claims
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR1913457A FR3103825B1 (fr) | 2019-11-29 | 2019-11-29 | Dispositif et procédé d’exposition de cellules a un aérosol |
PCT/EP2020/080282 WO2021104787A1 (fr) | 2019-11-29 | 2020-10-28 | Dispositif et procédé d'exposition de cellules à un aérosol |
Publications (1)
Publication Number | Publication Date |
---|---|
EP4041860A1 true EP4041860A1 (fr) | 2022-08-17 |
Family
ID=71094402
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP20797761.2A Withdrawn EP4041860A1 (fr) | 2019-11-29 | 2020-10-28 | Dispositif et procédé d'exposition de cellules à un aérosol |
Country Status (3)
Country | Link |
---|---|
EP (1) | EP4041860A1 (fr) |
FR (1) | FR3103825B1 (fr) |
WO (1) | WO2021104787A1 (fr) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20200407675A1 (en) * | 2019-06-25 | 2020-12-31 | Aerosol Dynamics Inc. | Efficient deposition of nano-sized particles onto cells at an air liquid interface |
CN113640475A (zh) * | 2021-09-16 | 2021-11-12 | 朱远贵 | 一种有机肥检测装置 |
WO2023073658A1 (fr) * | 2021-10-29 | 2023-05-04 | Universidade Do Algarve | Dispositif portatif pour l'étude de l'exposition de cellules à des poudres sèches |
CN115032123B (zh) * | 2022-03-21 | 2024-06-25 | 哈尔滨工程大学 | 一种研究不同热工条件下管道内气溶胶沉积特性的实验装置 |
WO2023235205A1 (fr) * | 2022-05-31 | 2023-12-07 | Rowan University | Dispositif d'essai de fluides vaporisables dans un modèle des voies respiratoires humaines |
CN115093943A (zh) * | 2022-06-09 | 2022-09-23 | 辽宁工程技术大学 | 一种离心撞击法生物气溶胶富集装置及其细胞暴露与应用 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2010239916A (ja) * | 2009-04-08 | 2010-10-28 | Lion Corp | 培養容器用スペーサ、該スペーサを備えた培養装置、該培養装置を用いた観察方法 |
US11001797B2 (en) | 2012-04-13 | 2021-05-11 | President And Fellows Of Harvard College | Devices and methods for in vitro aerosol delivery |
CN203754702U (zh) * | 2014-03-15 | 2014-08-06 | 北京慧荣和科技有限公司 | 单浓度细胞暴露系统 |
US20180171280A1 (en) | 2016-11-25 | 2018-06-21 | Government of the United States as represented by the Administrator of the U.S. Environmental Protec | Cell culture exposure system (cces) |
-
2019
- 2019-11-29 FR FR1913457A patent/FR3103825B1/fr active Active
-
2020
- 2020-10-28 EP EP20797761.2A patent/EP4041860A1/fr not_active Withdrawn
- 2020-10-28 WO PCT/EP2020/080282 patent/WO2021104787A1/fr unknown
Also Published As
Publication number | Publication date |
---|---|
FR3103825B1 (fr) | 2021-12-03 |
FR3103825A1 (fr) | 2021-06-04 |
WO2021104787A1 (fr) | 2021-06-03 |
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