EP3963042A1 - Dispositif lumineux et thermique pour l'optimisation de la production de biomasse dans des bioréacteurs - Google Patents

Dispositif lumineux et thermique pour l'optimisation de la production de biomasse dans des bioréacteurs

Info

Publication number
EP3963042A1
EP3963042A1 EP20724429.4A EP20724429A EP3963042A1 EP 3963042 A1 EP3963042 A1 EP 3963042A1 EP 20724429 A EP20724429 A EP 20724429A EP 3963042 A1 EP3963042 A1 EP 3963042A1
Authority
EP
European Patent Office
Prior art keywords
light
temperature device
led light
incubation container
arrangement
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP20724429.4A
Other languages
German (de)
English (en)
Inventor
Horst Theisen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rhenac Greentec AG
Original Assignee
Rhenac Greentec AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rhenac Greentec AG filed Critical Rhenac Greentec AG
Publication of EP3963042A1 publication Critical patent/EP3963042A1/fr
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00—Means for providing, directing, scattering or concentrating light
    • C12M31/02—Means for providing, directing, scattering or concentrating light located outside the reactor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00—Means for providing, directing, scattering or concentrating light
    • C12M31/08—Means for providing, directing, scattering or concentrating light by conducting or reflecting elements located inside the reactor or in its structure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00—Means for providing, directing, scattering or concentrating light
    • C12M31/10—Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/06—Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes

Definitions

  • the invention relates to a light and temperature device for increasing and optimizing the production of biomass in bioreactors.
  • the invention relates to the provision of a light and temperature cuff for the production of microorganisms such as microalgae in photobioreactors.
  • microorganisms such as microalgae in photobioreactors.
  • microorganisms In such photobioreactors, predominantly microorganisms are multiplied and / or cultivated with light, CO 2 , nutrients as well as water and salts.
  • These microorganisms or their components are mainly used to extract oils, fuels, food, food additives, enzymes and proteins, pharmacological agents, chemical products, plastics, or are used in aquaculture, in animal feed, environmental technology and for cosmetics and care.
  • a bioreactor is a plant for the production of microorganisms outside of their natural and within an artificial technical environment.
  • the prefix “Photo” describes the ability of the bioreactor to cultivate phototrophs, that is, to use light for organisms that use their own energy. These organisms use the process of photosynthesis to build their own biomass from light and CO 2 . These organisms include plants, mosses, macroalgae, microalgae, cyanobacteria and purple bacteria.
  • the main goal of a photobioreactor is the controlled provision of a habitat that offers the optimal living conditions for the respective organism.
  • a photobioreactor enables significantly higher growth rates and purities than would be the case in a natural or nature-like environment.
  • Photobioreactors are therefore systems for the cultivation of mainly different eukaryotic organisms that live in water and carry out photosynthesis, such as microalgae.
  • Such systems are known from the prior art.
  • a widespread system for cultivating microorganisms is, for example, a tube circulation system, consisting of tubes that, by changing direction in repetitive loops, form a quasi-endless loop. In these tubes will be Cultivated microorganisms are kept in circulation and in motion by means of pump systems. In the closed circuit, the microorganisms grow - through the addition of carbon dioxide and nutrients that are brought into the system.
  • the growing phototrophic microorganisms also need light in order to carry out photosynthesis and to be able to grow.
  • the light is a decisive factor here for the optimal productivity of the entire phototrophic microorganisms or the entire system.
  • additional light is usually used.
  • light sources such as sodium vapor lamps or metal halide lamps are attached as additional exposure around the entire tube system or above this system.
  • the tube photobioreactors are additionally heated, which has proven to be unfavorable for the development of phototrophic microorganism cultures; the microalgae cultures tend to adhere to the glass tube surfaces as they heat up.
  • the object of the invention is therefore to overcome the disadvantages of the previously described photobioreactors, in particular that of the tube photobioreactors.
  • the aim is to improve the culture conditions for the growth of phototrophic microorganisms or other biomass producers, in particular by optimizing the availability of light within such a photobioreactor.
  • Another object of the invention is to set the temperature conditions as optimally as possible for the phototrophic microorganisms.
  • the objects are achieved by a light and temperature device according to the invention for optimizing the production of biomass in bioreactors according to the independent claim 1.
  • Advantageous developments and preferred designs are specified in the subclaims.
  • the LED light arrangement is arranged in relation to the incubation container in such a way that light radiation emanating from the LED light arrangement is directed towards the incubation container.
  • the cooling fins of the light arrangement are arranged so that they point away from the LED light arrays. The distance which can be present between the light arrangement and the incubation container is advantageously kept as small as possible from a structural point of view.
  • the incubation systems e.g. Tube systems can be converted from glass to plastic, because the constant temperature allows the expansion of the (tube) systems to be controlled. This also lowers the costs for the production of the bioreactor systems.
  • the light and temperature device according to the invention can be expected to increase the organic dry matter from currently around 10-15 grams / liter of circulating liquid to around 25-30 grams / liter.
  • the (tube) bioreactors could be designed with larger (tube) diameters, which significantly improves the plant productivity per unit area.
  • the arrangement of the cooling ribs behind the LED light arrays, with these pointing away from the LED light arrays, has the advantage that the waste heat from the LED light arrays can be dissipated away from the incubation container. This in turn has the consequence that the incubation container and thus the ones in it Microorganisms are not additionally heated and this results in unfavorable culture conditions for the microorganisms.
  • the releasable attachment of the light arrangement by at least one clamp has the advantage that the light and temperature device according to the invention can be adapted to the scaling of a photobioreactor system simply, quickly and as required.
  • the at least one fastening means of the light and temperature device according to the invention can also be constructed from various components.
  • the fastening means per se can represent any type of fastening means as long as it is suitable for the releasable attachment of the light and temperature device according to the invention to a translucent incubation container. It may be advantageous to use more than one fastening means in order to fasten several LED light assemblies to a translucent incubation container or to increase the stability of the entire light and temperature device which is fastened to the incubation container.
  • the fastening means can also be used to accommodate further optional elements such as measuring and / or monitoring devices.
  • the fastening means is a clamp.
  • microorganisms are understood to mean plants, mosses, macroalgae, cyanobacteria, purple bacteria, plankton and, above all, microalgae.
  • the light and temperature device according to the invention is preferably used for the cultivation of microalgae and is designed for this.
  • a bioreactor system or a photobioreactor system is understood to mean a system which is made up of various components, for example pipes, plates, foils or other components suitable for growing microorganisms.
  • a photobioreactor system includes pumps, inlet and outlet valves for nutrients, CO2, salts, water, etc. as well as fastening means for suspending or connecting the individual components.
  • an incubation container is understood to mean a component of a bioreactor system which mainly serves to store the microorganisms to multiply and / or cultivate.
  • Such an incubation container can have various geometric shapes. Tubes, plates, foils and / or hoses have proven to be particularly advantageous.
  • phototrophic microorganisms these are of course made of a translucent material so that the microorganisms can photosynthesize.
  • bioreactors have proven to be constructed as a tube photobioreactor, Christmas tree photobioreactor, plate photobioreactor or film photobioreactor.
  • the present light and temperature device according to the invention can be applied to this as well as to all common photobioreactors.
  • the light and temperature device is designed in the form of a sleeve. That is, the light and temperature device can be arranged or installed around an incubation container (e.g. arrangement around the tube lengths).
  • This light and temperature device designed as a sleeve and equipped with LED light arrays, ensures the best possible light supply for the microorganism cultures.
  • such a cuff has further components such as casing or sheathing parts in order to surround the individual components of the cuff around an incubation container, e.g. to arrange a tube and fix it with suitable fasteners (e.g. a clamp).
  • the light and temperature device comprises at least one channel for connecting cooling water or cooling liquid.
  • This channel runs through the LED light arrangement and can therefore be used for active cooling of the LEDs.
  • active cooling can be operated, for example, on hot days or more intensive light irradiation, and thus an optimal temperature level can be created for the multiplication of the microorganism culture.
  • a desired temperature input can be generated in the incubation container (e.g. tube system), so that the same climate can be cultivated all year round, regardless of location.
  • the device has suitable connections for the supply and discharge of water or coolant.
  • it has at least one electrical interface for connecting measuring and / or monitoring devices.
  • measuring and / or monitoring devices can include, for example, various cameras, such as CCD cameras, temperature sensors, spectrometers, viscometers and fluorescence and chlorophyll measuring devices.
  • Such measuring and / or monitoring devices can be designed to measure fluorescence, luminescence, radiation, fluidity, viscosity, color changes and / or chlorophyll content.
  • the advantage of attaching such measuring and / or monitoring devices to the light and / or temperature device according to the invention is that they are suitable, for example, to determine or to determine the optimal use of light, the harvest time of the microorganism culture and / or possibly possible contamination of the culture detect.
  • the active regulation of the temperature for example via cooling water or cooling liquid, which can then be pumped into the channels of the LED light arrangement, can also be regulated, for example with a temperature sensor.
  • the light and temperature device has insulation between and / or to the side of the LED light arrangement and the incubation container.
  • the insulation is applied in such a way that the light irradiation of the LED light arrangement on the incubation container is not influenced by this.
  • Such insulation includes, for example, rubber, plastic, polyurethanes (PUR) or polyisocyanurate rigid foam (PIR).
  • the fastening means can also have insulation or a special insulation is held around the incubation container by means of a fastening means.
  • An insulation can also completely or partially encase or cover the entire incubation container - with the exception of the LED light unit.
  • the insulation variants have the advantage that, for example, the incubation container is protected from damage by possibly metallic or hard components of the light and Temperature device is not damaged or scratched.
  • Another advantage of such an insulation for example if it is made of rubber or the like, is that it also achieves a certain slip resistance, which prevents twisting of the light and temperature device according to the invention, in particular the LED light arrangement, relative to the incubation container.
  • the light and temperature device can have one or more LED light arrangements which are arranged continuously around a longitudinal axis of an incubation container.
  • these LED light arrangements can be arranged around the incubation container at winding intervals of 1 to 360 degrees.
  • the LED light arrangements are arranged continuously around the longitudinal axis of an incubation container at angular intervals of 180 degrees or 135 degrees or preferably 90 degrees.
  • the LED light arrangement is designed to emit a fixed light spectrum, also referred to as an SMD version (surface-mounted devices), or to emit a variable light spectrum.
  • SMD version surface-mounted devices
  • a changeable light spectrum which is also referred to as a multichannel version
  • each light color receives a separate channel to control 0-100% light output.
  • the spectrum can then be controlled variably, based on requirements.
  • the light and temperature device it is designed in such a way that the smallest possible structural distance is between the LED light arrangement or the LED light arrays and the incubation container.
  • This structural variant has the advantage that the energy input for the exposure of the microorganism culture can be reduced considerably.
  • this is for detachable attachment to a tubular, cylindrical, cuboid, prism-shaped, pentahedral, hexahedral, heptahedral, octahedral or other geometrically shaped elongated incubation container formed.
  • the light and temperature device is made from materials that are suitable for passive cooling.
  • components of the light and temperature device have sections made of aluminum or other metals or materials suitable for cooling.
  • the cooling fins of the LED light arrangement are made from such a material.
  • FIG. 1 a schematic three-dimensional representation of the light and temperature device according to the invention according to an advantageous embodiment in the attached state around an incubation container;
  • FIG. 2 shows a schematic representation of a cross section of the light and temperature device according to the invention from FIG. 1 ;
  • FIG. 3 shows an enlarged schematic representation of the LED light arrangement which is attached to the incubation container according to section X from FIG. 2.
  • FIG. 4 is a schematic side view of the light and temperature device according to the invention according to FIG. 1 ;
  • FIG. 5 a schematic representation in a plan view of the light and temperature device according to the invention according to FIG. 1 ;
  • FIG. 1 is a preferred embodiment of the light and temperature device 10 according to the invention in a schematic three-dimensional representation.
  • the light and temperature device 10 according to the invention is already in the assembled state on an opaque incubation container 40.
  • the incubation container 40 is a plexiglass tube, which can be built into a so-called tube photobioreactor via deflection modules with other plexiglass tubes.
  • the light and temperature device 10 according to the invention has two opposing LED light arrangements 20. Furthermore, in this embodiment, the light and temperature device 10 according to the invention is designed in the form of a sleeve. This is formed around the incubation container by means of sheathing components, in this case quarter shells 70, and clamps provided for this purpose as fastening means.
  • FIG. 2 shows the embodiment from FIG. 1 in a schematic cross section.
  • the two LED light arrangements 20 as well as the quarter shells 70 can be seen.
  • the quarter shells 70 also fasten an insulation material 60 around the incubation container 40 by means of the clamps 30.
  • FIG. 3 shows, in an enlarged area, a schematic cross section of the LED light arrangement 20 from FIG. 2.
  • the LED light arrangement 20 can be seen here, which has cooling fins 22 and LED light arrays 21.
  • a connection for water or cooling liquid can also be seen, which further opens into a channel 50 which runs through the LED light arrangement 20, in this case through the cooling rib profile 22.
  • a special clamp 30 for holding the cooling profile 22 on the incubation container 40 can also be seen.
  • FIG. 5 The special embodiment of the invention Licht- und Temperaturvorrich device 10 according to FIG. 1 is shown in FIG. 5 in plan view and in FIG. 4 can be seen in side view.
  • This special embodiment of the light and temperature device 10 according to the invention, described in FIGS. 1-5, is particularly suitable for the design in tube photobioreactors.
  • Plexiglass tubes for example, can be used as induction containers 40 for this purpose.
  • these tubes 40 are provided with the light and temperature device 10 according to the invention, which forms the shape of a sleeve.
  • This embodiment is also called a light cuff designated.
  • the light cuffs can be placed around the tubes. These are releasably attached again by means of quarter shells 70 and special clamps 30.
  • the light sleeves contain LED light arrangements 20, which can couple light into the tubes directly, on one side or, as in this case, on both sides offset by 180 degrees.
  • the light sleeves are preferably made of aluminum or other materials suitable for cooling.
  • the LED light arrangements 20 in this exemplary embodiment have cooling ribs 22 on the outside of the light arrangement 20 in order to be able to dissipate the resulting waste heat from the LEDs to the outside.
  • the LED light arrangements 20 have cooling channels 50 with connections into which cooling liquid or water can be introduced.
  • the temperature of the cooling liquid can be regulated via a connection to refrigeration systems, heat exchangers or the like. This enables adjustable and controlled temperatures on the tubes, which has a positive effect on the production of the microorganism culture.
  • the light arrangements 20 equipped with LED light arrays 21 ensure the best possible light supply for the microorganism cultures.
  • the LED light arrangement 20 can be designed as an SMD version with a fixed light spectrum or as a multichannel version, with each light color receiving a separate channel for controlling 0-100% light output. Such a spectrum can then be controlled variably, based on requirements.
  • the energy consumption can be reduced by bringing the LED light source as close as possible to the tube without any distance.
  • the use of energy can also be reduced if the special light spectrum is tailored to the microorganisms to be cultivated. With an arrangement described in this specific embodiment, there is also no inherent shading by the tube arrangement itself.
  • the light sleeves can be used in different sizes for different tube diameters.
  • the LED light sources can be perfectly matched to the photosynthesis spectrum of the microorganism cultures, which can be achieved, for example, with the aid of various measuring and / or monitoring devices.
  • a mini camera can be used to measure fluorescence, which can be used to optimally determine the optimal use of light, the time of harvest and other parameters.
  • a temperature sensor can also be used, for example, to measure the optimum temperature for the microorganism culture and, if necessary, can be actively adapted to the LED light arrangement via the cooling channels.

Abstract

Dispositif lumineux et thermique pour l'augmentation et l'optimisation de la production de biomasse dans des bioréacteurs, celui-ci présentant un agencement de lumières LED comportant des réseaux de lumières LED et des ailettes de refroidissement, qui pointent à l'opposé des réseaux de lumières LED. Le dispositif lumineux et thermique comprend en outre au moins un moyen de fixation pour la montage détachable de l'agencement de lumières sur un récipient d'incubation transparent, l'agencement de lumières LED étant orienté vers le récipient d'incubation de telle sorte qu'un rayonnement lumineux provenant de l'agencement de lumières LED est dirigé vers le récipient d'incubation.
EP20724429.4A 2019-04-29 2020-04-28 Dispositif lumineux et thermique pour l'optimisation de la production de biomasse dans des bioréacteurs Pending EP3963042A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102019110989.2A DE102019110989A1 (de) 2019-04-29 2019-04-29 Licht- und Temperaturvorrichtung zur Optimierung der Produktion von Biomasse in Bioreaktoren
PCT/EP2020/061787 WO2020221761A1 (fr) 2019-04-29 2020-04-28 Dispositif lumineux et thermique pour l'optimisation de la production de biomasse dans des bioréacteurs

Publications (1)

Publication Number Publication Date
EP3963042A1 true EP3963042A1 (fr) 2022-03-09

Family

ID=70613733

Family Applications (1)

Application Number Title Priority Date Filing Date
EP20724429.4A Pending EP3963042A1 (fr) 2019-04-29 2020-04-28 Dispositif lumineux et thermique pour l'optimisation de la production de biomasse dans des bioréacteurs

Country Status (4)

Country Link
US (1) US20220204897A1 (fr)
EP (1) EP3963042A1 (fr)
DE (1) DE102019110989A1 (fr)
WO (1) WO2020221761A1 (fr)

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7220018B2 (en) * 2003-12-15 2007-05-22 Orbital Technologies, Inc. Marine LED lighting system and method
DE202006003878U1 (de) * 2005-03-16 2006-06-14 Sartorius Ag Beleuchtungseinrichtung zur Beleuchtung für die Kultivierung von phototrophen Zellkulturen in Bioreaktoren
JP2008237067A (ja) * 2007-03-26 2008-10-09 Ccs Inc 藻類培養システム
DE202007013406U1 (de) * 2007-09-25 2007-11-22 Lehr, Florian, Dipl.-Ing. Beleuchtungseinrichtung für Bioreaktoren
WO2009141287A1 (fr) * 2008-05-22 2009-11-26 Syddansk Universitet Procédé et appareil permettant d'utiliser des diodes électroluminescentes dans une serre
KR20160081301A (ko) * 2014-12-31 2016-07-08 (주)웰니스앤 Led 광원위치 제어를 이용한 간편 미세조류 배양기
DE102017008769B4 (de) * 2017-09-19 2022-03-10 Sartorius Stedim Biotech Gmbh Beleuchtung für einen Einweg-Photo-Bioreaktor

Also Published As

Publication number Publication date
US20220204897A1 (en) 2022-06-30
DE102019110989A1 (de) 2020-10-29
WO2020221761A1 (fr) 2020-11-05

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