EP3963042A1 - Light and temperature device for optimizing the production of biomass in bioreactors - Google Patents

Light and temperature device for optimizing the production of biomass in bioreactors

Info

Publication number
EP3963042A1
EP3963042A1 EP20724429.4A EP20724429A EP3963042A1 EP 3963042 A1 EP3963042 A1 EP 3963042A1 EP 20724429 A EP20724429 A EP 20724429A EP 3963042 A1 EP3963042 A1 EP 3963042A1
Authority
EP
European Patent Office
Prior art keywords
light
temperature device
led light
incubation container
arrangement
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP20724429.4A
Other languages
German (de)
French (fr)
Inventor
Horst Theisen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rhenac Greentec AG
Original Assignee
Rhenac Greentec AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rhenac Greentec AG filed Critical Rhenac Greentec AG
Publication of EP3963042A1 publication Critical patent/EP3963042A1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00—Means for providing, directing, scattering or concentrating light
    • C12M31/02—Means for providing, directing, scattering or concentrating light located outside the reactor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00—Means for providing, directing, scattering or concentrating light
    • C12M31/08—Means for providing, directing, scattering or concentrating light by conducting or reflecting elements located inside the reactor or in its structure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00—Means for providing, directing, scattering or concentrating light
    • C12M31/10—Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/06—Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes

Definitions

  • the invention relates to a light and temperature device for increasing and optimizing the production of biomass in bioreactors.
  • the invention relates to the provision of a light and temperature cuff for the production of microorganisms such as microalgae in photobioreactors.
  • microorganisms such as microalgae in photobioreactors.
  • microorganisms In such photobioreactors, predominantly microorganisms are multiplied and / or cultivated with light, CO 2 , nutrients as well as water and salts.
  • These microorganisms or their components are mainly used to extract oils, fuels, food, food additives, enzymes and proteins, pharmacological agents, chemical products, plastics, or are used in aquaculture, in animal feed, environmental technology and for cosmetics and care.
  • a bioreactor is a plant for the production of microorganisms outside of their natural and within an artificial technical environment.
  • the prefix “Photo” describes the ability of the bioreactor to cultivate phototrophs, that is, to use light for organisms that use their own energy. These organisms use the process of photosynthesis to build their own biomass from light and CO 2 . These organisms include plants, mosses, macroalgae, microalgae, cyanobacteria and purple bacteria.
  • the main goal of a photobioreactor is the controlled provision of a habitat that offers the optimal living conditions for the respective organism.
  • a photobioreactor enables significantly higher growth rates and purities than would be the case in a natural or nature-like environment.
  • Photobioreactors are therefore systems for the cultivation of mainly different eukaryotic organisms that live in water and carry out photosynthesis, such as microalgae.
  • Such systems are known from the prior art.
  • a widespread system for cultivating microorganisms is, for example, a tube circulation system, consisting of tubes that, by changing direction in repetitive loops, form a quasi-endless loop. In these tubes will be Cultivated microorganisms are kept in circulation and in motion by means of pump systems. In the closed circuit, the microorganisms grow - through the addition of carbon dioxide and nutrients that are brought into the system.
  • the growing phototrophic microorganisms also need light in order to carry out photosynthesis and to be able to grow.
  • the light is a decisive factor here for the optimal productivity of the entire phototrophic microorganisms or the entire system.
  • additional light is usually used.
  • light sources such as sodium vapor lamps or metal halide lamps are attached as additional exposure around the entire tube system or above this system.
  • the tube photobioreactors are additionally heated, which has proven to be unfavorable for the development of phototrophic microorganism cultures; the microalgae cultures tend to adhere to the glass tube surfaces as they heat up.
  • the object of the invention is therefore to overcome the disadvantages of the previously described photobioreactors, in particular that of the tube photobioreactors.
  • the aim is to improve the culture conditions for the growth of phototrophic microorganisms or other biomass producers, in particular by optimizing the availability of light within such a photobioreactor.
  • Another object of the invention is to set the temperature conditions as optimally as possible for the phototrophic microorganisms.
  • the objects are achieved by a light and temperature device according to the invention for optimizing the production of biomass in bioreactors according to the independent claim 1.
  • Advantageous developments and preferred designs are specified in the subclaims.
  • the LED light arrangement is arranged in relation to the incubation container in such a way that light radiation emanating from the LED light arrangement is directed towards the incubation container.
  • the cooling fins of the light arrangement are arranged so that they point away from the LED light arrays. The distance which can be present between the light arrangement and the incubation container is advantageously kept as small as possible from a structural point of view.
  • the incubation systems e.g. Tube systems can be converted from glass to plastic, because the constant temperature allows the expansion of the (tube) systems to be controlled. This also lowers the costs for the production of the bioreactor systems.
  • the light and temperature device according to the invention can be expected to increase the organic dry matter from currently around 10-15 grams / liter of circulating liquid to around 25-30 grams / liter.
  • the (tube) bioreactors could be designed with larger (tube) diameters, which significantly improves the plant productivity per unit area.
  • the arrangement of the cooling ribs behind the LED light arrays, with these pointing away from the LED light arrays, has the advantage that the waste heat from the LED light arrays can be dissipated away from the incubation container. This in turn has the consequence that the incubation container and thus the ones in it Microorganisms are not additionally heated and this results in unfavorable culture conditions for the microorganisms.
  • the releasable attachment of the light arrangement by at least one clamp has the advantage that the light and temperature device according to the invention can be adapted to the scaling of a photobioreactor system simply, quickly and as required.
  • the at least one fastening means of the light and temperature device according to the invention can also be constructed from various components.
  • the fastening means per se can represent any type of fastening means as long as it is suitable for the releasable attachment of the light and temperature device according to the invention to a translucent incubation container. It may be advantageous to use more than one fastening means in order to fasten several LED light assemblies to a translucent incubation container or to increase the stability of the entire light and temperature device which is fastened to the incubation container.
  • the fastening means can also be used to accommodate further optional elements such as measuring and / or monitoring devices.
  • the fastening means is a clamp.
  • microorganisms are understood to mean plants, mosses, macroalgae, cyanobacteria, purple bacteria, plankton and, above all, microalgae.
  • the light and temperature device according to the invention is preferably used for the cultivation of microalgae and is designed for this.
  • a bioreactor system or a photobioreactor system is understood to mean a system which is made up of various components, for example pipes, plates, foils or other components suitable for growing microorganisms.
  • a photobioreactor system includes pumps, inlet and outlet valves for nutrients, CO2, salts, water, etc. as well as fastening means for suspending or connecting the individual components.
  • an incubation container is understood to mean a component of a bioreactor system which mainly serves to store the microorganisms to multiply and / or cultivate.
  • Such an incubation container can have various geometric shapes. Tubes, plates, foils and / or hoses have proven to be particularly advantageous.
  • phototrophic microorganisms these are of course made of a translucent material so that the microorganisms can photosynthesize.
  • bioreactors have proven to be constructed as a tube photobioreactor, Christmas tree photobioreactor, plate photobioreactor or film photobioreactor.
  • the present light and temperature device according to the invention can be applied to this as well as to all common photobioreactors.
  • the light and temperature device is designed in the form of a sleeve. That is, the light and temperature device can be arranged or installed around an incubation container (e.g. arrangement around the tube lengths).
  • This light and temperature device designed as a sleeve and equipped with LED light arrays, ensures the best possible light supply for the microorganism cultures.
  • such a cuff has further components such as casing or sheathing parts in order to surround the individual components of the cuff around an incubation container, e.g. to arrange a tube and fix it with suitable fasteners (e.g. a clamp).
  • the light and temperature device comprises at least one channel for connecting cooling water or cooling liquid.
  • This channel runs through the LED light arrangement and can therefore be used for active cooling of the LEDs.
  • active cooling can be operated, for example, on hot days or more intensive light irradiation, and thus an optimal temperature level can be created for the multiplication of the microorganism culture.
  • a desired temperature input can be generated in the incubation container (e.g. tube system), so that the same climate can be cultivated all year round, regardless of location.
  • the device has suitable connections for the supply and discharge of water or coolant.
  • it has at least one electrical interface for connecting measuring and / or monitoring devices.
  • measuring and / or monitoring devices can include, for example, various cameras, such as CCD cameras, temperature sensors, spectrometers, viscometers and fluorescence and chlorophyll measuring devices.
  • Such measuring and / or monitoring devices can be designed to measure fluorescence, luminescence, radiation, fluidity, viscosity, color changes and / or chlorophyll content.
  • the advantage of attaching such measuring and / or monitoring devices to the light and / or temperature device according to the invention is that they are suitable, for example, to determine or to determine the optimal use of light, the harvest time of the microorganism culture and / or possibly possible contamination of the culture detect.
  • the active regulation of the temperature for example via cooling water or cooling liquid, which can then be pumped into the channels of the LED light arrangement, can also be regulated, for example with a temperature sensor.
  • the light and temperature device has insulation between and / or to the side of the LED light arrangement and the incubation container.
  • the insulation is applied in such a way that the light irradiation of the LED light arrangement on the incubation container is not influenced by this.
  • Such insulation includes, for example, rubber, plastic, polyurethanes (PUR) or polyisocyanurate rigid foam (PIR).
  • the fastening means can also have insulation or a special insulation is held around the incubation container by means of a fastening means.
  • An insulation can also completely or partially encase or cover the entire incubation container - with the exception of the LED light unit.
  • the insulation variants have the advantage that, for example, the incubation container is protected from damage by possibly metallic or hard components of the light and Temperature device is not damaged or scratched.
  • Another advantage of such an insulation for example if it is made of rubber or the like, is that it also achieves a certain slip resistance, which prevents twisting of the light and temperature device according to the invention, in particular the LED light arrangement, relative to the incubation container.
  • the light and temperature device can have one or more LED light arrangements which are arranged continuously around a longitudinal axis of an incubation container.
  • these LED light arrangements can be arranged around the incubation container at winding intervals of 1 to 360 degrees.
  • the LED light arrangements are arranged continuously around the longitudinal axis of an incubation container at angular intervals of 180 degrees or 135 degrees or preferably 90 degrees.
  • the LED light arrangement is designed to emit a fixed light spectrum, also referred to as an SMD version (surface-mounted devices), or to emit a variable light spectrum.
  • SMD version surface-mounted devices
  • a changeable light spectrum which is also referred to as a multichannel version
  • each light color receives a separate channel to control 0-100% light output.
  • the spectrum can then be controlled variably, based on requirements.
  • the light and temperature device it is designed in such a way that the smallest possible structural distance is between the LED light arrangement or the LED light arrays and the incubation container.
  • This structural variant has the advantage that the energy input for the exposure of the microorganism culture can be reduced considerably.
  • this is for detachable attachment to a tubular, cylindrical, cuboid, prism-shaped, pentahedral, hexahedral, heptahedral, octahedral or other geometrically shaped elongated incubation container formed.
  • the light and temperature device is made from materials that are suitable for passive cooling.
  • components of the light and temperature device have sections made of aluminum or other metals or materials suitable for cooling.
  • the cooling fins of the LED light arrangement are made from such a material.
  • FIG. 1 a schematic three-dimensional representation of the light and temperature device according to the invention according to an advantageous embodiment in the attached state around an incubation container;
  • FIG. 2 shows a schematic representation of a cross section of the light and temperature device according to the invention from FIG. 1 ;
  • FIG. 3 shows an enlarged schematic representation of the LED light arrangement which is attached to the incubation container according to section X from FIG. 2.
  • FIG. 4 is a schematic side view of the light and temperature device according to the invention according to FIG. 1 ;
  • FIG. 5 a schematic representation in a plan view of the light and temperature device according to the invention according to FIG. 1 ;
  • FIG. 1 is a preferred embodiment of the light and temperature device 10 according to the invention in a schematic three-dimensional representation.
  • the light and temperature device 10 according to the invention is already in the assembled state on an opaque incubation container 40.
  • the incubation container 40 is a plexiglass tube, which can be built into a so-called tube photobioreactor via deflection modules with other plexiglass tubes.
  • the light and temperature device 10 according to the invention has two opposing LED light arrangements 20. Furthermore, in this embodiment, the light and temperature device 10 according to the invention is designed in the form of a sleeve. This is formed around the incubation container by means of sheathing components, in this case quarter shells 70, and clamps provided for this purpose as fastening means.
  • FIG. 2 shows the embodiment from FIG. 1 in a schematic cross section.
  • the two LED light arrangements 20 as well as the quarter shells 70 can be seen.
  • the quarter shells 70 also fasten an insulation material 60 around the incubation container 40 by means of the clamps 30.
  • FIG. 3 shows, in an enlarged area, a schematic cross section of the LED light arrangement 20 from FIG. 2.
  • the LED light arrangement 20 can be seen here, which has cooling fins 22 and LED light arrays 21.
  • a connection for water or cooling liquid can also be seen, which further opens into a channel 50 which runs through the LED light arrangement 20, in this case through the cooling rib profile 22.
  • a special clamp 30 for holding the cooling profile 22 on the incubation container 40 can also be seen.
  • FIG. 5 The special embodiment of the invention Licht- und Temperaturvorrich device 10 according to FIG. 1 is shown in FIG. 5 in plan view and in FIG. 4 can be seen in side view.
  • This special embodiment of the light and temperature device 10 according to the invention, described in FIGS. 1-5, is particularly suitable for the design in tube photobioreactors.
  • Plexiglass tubes for example, can be used as induction containers 40 for this purpose.
  • these tubes 40 are provided with the light and temperature device 10 according to the invention, which forms the shape of a sleeve.
  • This embodiment is also called a light cuff designated.
  • the light cuffs can be placed around the tubes. These are releasably attached again by means of quarter shells 70 and special clamps 30.
  • the light sleeves contain LED light arrangements 20, which can couple light into the tubes directly, on one side or, as in this case, on both sides offset by 180 degrees.
  • the light sleeves are preferably made of aluminum or other materials suitable for cooling.
  • the LED light arrangements 20 in this exemplary embodiment have cooling ribs 22 on the outside of the light arrangement 20 in order to be able to dissipate the resulting waste heat from the LEDs to the outside.
  • the LED light arrangements 20 have cooling channels 50 with connections into which cooling liquid or water can be introduced.
  • the temperature of the cooling liquid can be regulated via a connection to refrigeration systems, heat exchangers or the like. This enables adjustable and controlled temperatures on the tubes, which has a positive effect on the production of the microorganism culture.
  • the light arrangements 20 equipped with LED light arrays 21 ensure the best possible light supply for the microorganism cultures.
  • the LED light arrangement 20 can be designed as an SMD version with a fixed light spectrum or as a multichannel version, with each light color receiving a separate channel for controlling 0-100% light output. Such a spectrum can then be controlled variably, based on requirements.
  • the energy consumption can be reduced by bringing the LED light source as close as possible to the tube without any distance.
  • the use of energy can also be reduced if the special light spectrum is tailored to the microorganisms to be cultivated. With an arrangement described in this specific embodiment, there is also no inherent shading by the tube arrangement itself.
  • the light sleeves can be used in different sizes for different tube diameters.
  • the LED light sources can be perfectly matched to the photosynthesis spectrum of the microorganism cultures, which can be achieved, for example, with the aid of various measuring and / or monitoring devices.
  • a mini camera can be used to measure fluorescence, which can be used to optimally determine the optimal use of light, the time of harvest and other parameters.
  • a temperature sensor can also be used, for example, to measure the optimum temperature for the microorganism culture and, if necessary, can be actively adapted to the LED light arrangement via the cooling channels.

Abstract

The invention relates to a light and temperature device for increasing and optimizing the production of biomass in bioreactors, said device comprising an LED light arrangement with LED light arrays and cooling fins facing away from the LED light arrays. The light and temperature device further comprises at least one fastening means for removably fastening the light arrangement to a transparent incubation vessel, the LED light arrangement being oriented in relation to the incubation vessel such that light radiation emitted by the LED light arrangement is directed towards the incubation vessel.

Description

Licht- und Temperaturvorrichtung zur Optimierung der Produktion von Biomasse in Bioreaktoren Light and temperature device for optimizing the production of biomass in bioreactors
Beschreibung description
Die Erfindung betrifft eine Licht- und Temperaturvorrichtung zur Erhöhung und Optimierung der Produktion von Biomasse in Bioreaktoren. Insbesondere betrifft die Erfindung die Bereitstellung einer Licht- und Temperaturmanschette für die Produktion von Mikroorganismen wie z.B. Mikroalgen in Photobioreaktoren. In solchen Photobioreaktoren werden mit Licht, CO2, Nährstoffen sowie Wasser und Salzen vorwiegend Mikroorganismen vermehrt und/oder kultiviert. Diese Mikroorganismen bzw. deren Bestandteile dienen hauptsächlich zur Gewinnung von Ölen, Kraftstoffen, Nahrungsmitteln, Nahrungsmittelzusatzstoffen, Enzymen und Proteinen, pharmakologischen Wirkstoffen, chemischen Produkten, Kunststoffen, oder werden in Aquakulturen, in Tierfutter, der Umwelttechnik und für Kosmetik und Pflege genutzt. The invention relates to a light and temperature device for increasing and optimizing the production of biomass in bioreactors. In particular, the invention relates to the provision of a light and temperature cuff for the production of microorganisms such as microalgae in photobioreactors. In such photobioreactors, predominantly microorganisms are multiplied and / or cultivated with light, CO 2 , nutrients as well as water and salts. These microorganisms or their components are mainly used to extract oils, fuels, food, food additives, enzymes and proteins, pharmacological agents, chemical products, plastics, or are used in aquaculture, in animal feed, environmental technology and for cosmetics and care.
Ein Bioreaktor ist eine Anlage zur Produktion von Mikroorganismen außerhalb ihrer natürlichen und innerhalb einer künstlichen technischen Umgebung. Die Vorsilbe „Photo“ beschreibt die Eigenschaft des Bioreaktors zur Kultivierung von Phototrophen, das heißt Licht zur eigenen Energiegewinnung nutzenden Organismen zu dienen. Diese Organismen nutzen den Prozess der Photosynthese, um aus Licht und CO2 ihre eigene Biomasse aufzubauen. Zu diesen Organismen zählen Pflanzen, Moose, Makroalgen, Mikroalgen, Cyanobakterien und Purpurbakterien. Das Kernziel eines Photobioreaktors ist die kontrollierte Bereitstellung eines Lebensraums, der für den jeweiligen Organismus die optimalen Lebensbedingungen bietet. Damit ermöglicht ein Photobioreaktor deutlich höhere Wachstumsraten und Reinheiten, als es in einer natürlichen oder naturähnlichen Umgebung der Fall wäre. A bioreactor is a plant for the production of microorganisms outside of their natural and within an artificial technical environment. The prefix “Photo” describes the ability of the bioreactor to cultivate phototrophs, that is, to use light for organisms that use their own energy. These organisms use the process of photosynthesis to build their own biomass from light and CO 2 . These organisms include plants, mosses, macroalgae, microalgae, cyanobacteria and purple bacteria. The main goal of a photobioreactor is the controlled provision of a habitat that offers the optimal living conditions for the respective organism. A photobioreactor enables significantly higher growth rates and purities than would be the case in a natural or nature-like environment.
Photobioreaktoren sind also Systeme zur Kultivierung von hauptsächlich verschiedenen eukaryotischen Lebewesen, die im Wasser leben und Photosynthese betreiben, wie beispielsweise Mikroalgen. Solche Systeme sind aus dem Stand der Technik bekannt. Ein weit verbreitetes System zur Kultivierung von Mikroorganismen ist beispielsweise ein Röhrenumlaufsystem, bestehend aus Röhren, die durch Richtungsänderungen in wiederholenden Schleifen eine quasi Endlosschleife bilden. In diesen Röhren werden angezüchtete Mikroorganismen mittels Pumpsystemen in Umlauf und in Bewegung gehalten. In dem geschlossenen Kreislauf wachsen die Mikroorganismen - durch Zuführung von Kohlenstoffdioxid und Nährstoffen, die in das System eingetragen werden - heran. Photobioreactors are therefore systems for the cultivation of mainly different eukaryotic organisms that live in water and carry out photosynthesis, such as microalgae. Such systems are known from the prior art. A widespread system for cultivating microorganisms is, for example, a tube circulation system, consisting of tubes that, by changing direction in repetitive loops, form a quasi-endless loop. In these tubes will be Cultivated microorganisms are kept in circulation and in motion by means of pump systems. In the closed circuit, the microorganisms grow - through the addition of carbon dioxide and nutrients that are brought into the system.
Ebenfalls benötigen die heranwachsenden phototrophen Mikroorganismen hierzu Licht, um Photosynthese betreiben und wachsen zu können. Das Licht ist hierbei ein entscheidender Faktor für die optimale Produktivität der gesamten phototrophen Mikroorganismen bzw. des gesamten Systems. Üblicherweise wird, um den Faktor Licht ganzjährig auf der erforderlichen Lichtintensität zu halten, zusätzlich mit Ergänzungslicht gearbeitet. Dazu werden um das gesamte Röhrensystem oder oberhalb dieses Systems Lichtquellen wie Natriumdampflampen, Halogenmetalldampflampen als Zusatzbelichtung angebracht. Durch diese Zusatzbelichtung werden die Röhren- Photobioreaktoren jedoch zusätzlich erwärmt, was sich als ungünstig für die phototrophe Mikroorganismenkulturentwicklung erwiesen hat, die Mikroalgen-Kulturen neigen dabei zum anheften an den sich erwärmenden Glasröhren-Oberflächen. The growing phototrophic microorganisms also need light in order to carry out photosynthesis and to be able to grow. The light is a decisive factor here for the optimal productivity of the entire phototrophic microorganisms or the entire system. In order to keep the light factor at the required light intensity all year round, additional light is usually used. For this purpose, light sources such as sodium vapor lamps or metal halide lamps are attached as additional exposure around the entire tube system or above this system. However, through this additional exposure, the tube photobioreactors are additionally heated, which has proven to be unfavorable for the development of phototrophic microorganism cultures; the microalgae cultures tend to adhere to the glass tube surfaces as they heat up.
Ein weiterer Nachteil eines solchen Systems ist der, dass das Licht nur in Teilen die Röhren erreicht, da sich die Röhren gegenseitig verschatten. Dieser Effekt steigt noch an, wenn sich die phototrophen Mikroorganismenkulturen in der Umlauf-Kulturflüssigkeit vermehren und die Flüssigkeit zunehmend durch den höheren Chlorophyllanteil grün wird. Tritt dieser Effekt ein, erreichen nur noch wenige Lichtstrahlen das Innere der Röhren, hierdurch sinkt der effektive Strahlungsanteil, der an der Photosynthese teilnehmen kann und somit auch letztendlich die Ausbeute an verwertbaren Stoffen in der Biomasse. Another disadvantage of such a system is that the light only partially reaches the tubes because the tubes shade each other. This effect increases when the phototrophic microorganism cultures multiply in the circulating culture liquid and the liquid becomes increasingly green due to the higher chlorophyll content. If this effect occurs, only a few rays of light reach the inside of the tubes, which means that the effective proportion of radiation that can take part in photosynthesis decreases and thus ultimately the yield of usable substances in the biomass.
Die Aufgabe der Erfindung besteht deshalb darin, die Nachteile der zuvor beschriebenen Photobioreaktoren, insbesondere der der Röhren- Photobioreaktoren, zu überwinden. Hierbei soll die Kulturbedingung für das Wachstum von phototrophen Mikroorganismen oder anderen Biomasseproduzenten verbessert werden, insbesondere durch die Optimierung der Lichtverfügbarkeit innerhalb eines solchen Photobioreaktoren. The object of the invention is therefore to overcome the disadvantages of the previously described photobioreactors, in particular that of the tube photobioreactors. The aim is to improve the culture conditions for the growth of phototrophic microorganisms or other biomass producers, in particular by optimizing the availability of light within such a photobioreactor.
Eine weitere Aufgabe der Erfindung besteht darin, die Temperaturbedingungen möglichst optimal für die phototrophen Mikroorganismen einzustellen. Die Aufgaben werden durch eine erfindungsgemäße Licht- und Temperaturvorrichtung zur Optimierung der Produktion von Biomasse in Bioreaktoren gemäß dem unabhängi gen Anspruch 1 gelöst. In den Unteransprüchen sind vorteilhafte Weiterbildungen und bevorzugte Ausführungen angegeben. Another object of the invention is to set the temperature conditions as optimally as possible for the phototrophic microorganisms. The objects are achieved by a light and temperature device according to the invention for optimizing the production of biomass in bioreactors according to the independent claim 1. Advantageous developments and preferred designs are specified in the subclaims.
Erfindungsgemäß wird eine Licht- und Temperaturvorrichtung zur Optimierung der Produktion von Biomasse in Bioreaktoren, insbesondere von Photobioreaktoren, zur Verfügung gestellt, wobei die Vorrichtung hierbei eine LED-Lichtanordnung mit LED- Lichtarrays und Kühlrippen sowie mindestens ein Befestigungsmittel zur wiederlösbaren Anbringung der Lichtanordnung an einen lichtdurchlässigen Inkubationsbehälter umfasst. Die LED-Lichtanordnung ist dabei zum Inkubationsbehälter derart angeordnet, dass eine von der LED-Lichtanordnung ausgehende Lichtstrahlung zum Inkubationsbehälter gerichtet ist. Die Kühlrippen der Lichtanordnung sind dabei so angeordnet, dass sie von den LED-Lichtarrays wegzeigen. Vorteilhafterweise wird dabei der Abstand, weicher zwischen Lichtanordnung und Inkubationsbehälter vorhanden sein kann, so gering wie bautechnisch möglich gehalten. Dies hat den Vorteil, dass die Lichtstrahlung nahezu ohne Verluste in den Inkubationsbehälter eingebracht wird. Dadurch wird eine Verschattung durch andere benachbarte Inkubationsbehälter vermieden und es kann eine optimale Lichtabgabe an die jeweils einzelnen Inkubationsbehälter eines zusammengesetzten Photobioreaktors erzielt werden. Durch die kontrollierten Temperaturbedingungen, welche durch die erfindungsgemäße Licht- und Temperaturvorrichtung zur Verfügung stehen, können auch die Inkubationssysteme z.B. Röhrensysteme, von Glas auf Kunststoff umgestellt werden, da durch die gleichbleibende Temperatur die Ausdehnung der (Röhren-)Systeme kontrolliert werden kann. Dies senkt zusätzlich die Kosten für die Herstellung der Bioreaktorsysteme. Die erfindungsgemäße Licht- und Temperaturvorrichtung lässt eine Steigerung der Bio- Trockenmasse von derzeit rund 10-15 Gramm/Liter umlaufende Flüssigkeit auf rund 25- 30 Gramm/Liter erwarten. Durch die Erfindung könnten die (Röhren-)Bioreaktoren mit größeren (Rohr-)Durchmessern ausgelegt werden, wodurch sich die Anlagenproduktivität pro Flächeneinheit deutlich verbessert. According to the invention, a light and temperature device for optimizing the production of biomass in bioreactors, in particular photobioreactors, is provided, the device in this case being an LED light arrangement with LED light arrays and cooling fins as well as at least one fastening means for releasable attachment of the light arrangement to a translucent one Incubation container includes. The LED light arrangement is arranged in relation to the incubation container in such a way that light radiation emanating from the LED light arrangement is directed towards the incubation container. The cooling fins of the light arrangement are arranged so that they point away from the LED light arrays. The distance which can be present between the light arrangement and the incubation container is advantageously kept as small as possible from a structural point of view. This has the advantage that the light radiation is introduced into the incubation container with almost no losses. This avoids shading by other neighboring incubation containers and an optimal emission of light to the respective individual incubation containers of an assembled photobioreactor can be achieved. Due to the controlled temperature conditions, which are available by the light and temperature device according to the invention, the incubation systems e.g. Tube systems can be converted from glass to plastic, because the constant temperature allows the expansion of the (tube) systems to be controlled. This also lowers the costs for the production of the bioreactor systems. The light and temperature device according to the invention can be expected to increase the organic dry matter from currently around 10-15 grams / liter of circulating liquid to around 25-30 grams / liter. As a result of the invention, the (tube) bioreactors could be designed with larger (tube) diameters, which significantly improves the plant productivity per unit area.
Die Anordnung der Kühlrippen hinter den LED-Lichtarrays, wobei diese von den LED- Lichtarrays wegzeigen, hat den Vorteil, dass hierdurch die Abwärme der LED- Lichtarrays weg vom Inkubationsbehälter abgeführt werden kann. Dies wiederum hat zur Folge, dass sich der Inkubationsbehälter und dadurch die darin befindlichen Mikroorganismen nicht zusätzlich erwärmt werden und sich für die Mikroorganismen hierdurch ungünstige Kulturbedingungen ergeben. The arrangement of the cooling ribs behind the LED light arrays, with these pointing away from the LED light arrays, has the advantage that the waste heat from the LED light arrays can be dissipated away from the incubation container. This in turn has the consequence that the incubation container and thus the ones in it Microorganisms are not additionally heated and this results in unfavorable culture conditions for the microorganisms.
Die wieder lösbare Anbringung der Lichtanordnung durch mindestens eine Klemme hat den Vorteil, dass die erfindungsgemäße Licht- und Temperaturvorrichtung einfach, schnell und bedarfsgerecht an die Skalierung eines Photobioreaktorsystems angepasst werden kann. The releasable attachment of the light arrangement by at least one clamp has the advantage that the light and temperature device according to the invention can be adapted to the scaling of a photobioreactor system simply, quickly and as required.
Das mindestens eine Befestigungsmittel der erfindungsgemäßen Licht- und Temperaturvorrichtung kann hierbei auch aus verschiedenen Komponenten aufgebaut sein. Das Befestigungsmittel an sich kann dabei jede Art von Befestigungsmittel darstellen, solange es zur wiederlösbaren Anbringung der erfindungsgemäßen Licht- und Temperaturvorrichtung an einen lichtdurchlässigen Inkubationsbehälter geeignet ist. Gegebenenfalls kann es von Vorteil sein, mehr als ein Befestigungsmittel zu verwenden, um mehrere LED-Lichtanordnungen an einem lichtdurchlässigen Inkubationsbehälter zu befestigen oder die Stabilität der gesamten Licht- und Temperaturvorrichtung, welche am Inkubationsbehälter befestigt wird, zu erhöhen. Weiter kann das Befestigungsmittel auch dazu dienen, weitere optionale Elemente wie beispielsweise Mess- und/oder Überwachungsgeräte aufzunehmen. Gemäß einer vorteilhaften Ausführungsform der erfindungsgemäßen Licht- und Temperaturvorrichtung ist das Befestigungsmittel eine Klemme. The at least one fastening means of the light and temperature device according to the invention can also be constructed from various components. The fastening means per se can represent any type of fastening means as long as it is suitable for the releasable attachment of the light and temperature device according to the invention to a translucent incubation container. It may be advantageous to use more than one fastening means in order to fasten several LED light assemblies to a translucent incubation container or to increase the stability of the entire light and temperature device which is fastened to the incubation container. Furthermore, the fastening means can also be used to accommodate further optional elements such as measuring and / or monitoring devices. According to an advantageous embodiment of the light and temperature device according to the invention, the fastening means is a clamp.
Im Sinne der Erfindung werden unter Mikroorganismen Pflanzen, Moose, Makroalgen, Cyanobakterien, Purpurbakterien, Plankton und vor allem Mikroalgen verstanden. Bevorzugt wird die erfindungsgemäße Licht- und Temperaturvorrichtung zur Kultivierung von Mikroalgen verwendet und ist dafür ausgelegt. For the purposes of the invention, microorganisms are understood to mean plants, mosses, macroalgae, cyanobacteria, purple bacteria, plankton and, above all, microalgae. The light and temperature device according to the invention is preferably used for the cultivation of microalgae and is designed for this.
Im Sinne der Erfindung wird unter einem Bioreaktorsystem oder einem Photobioreaktorsystem ein System verstanden, das aus verschiedenen Komponenten, beispielsweise aus Rohren, Platten, Folien oder anderen für die Aufzucht von Mikroorganismen geeigneten Bauteilen aufgebaut ist. Zudem umfasst solch ein Photobioreaktorsystem Pumpen, Einlass- und Auslassventile für Nährstoffe, CO2, Salze, Wasser etc. sowie Befestigungsmittel zur Aufhängung oder Verbindung der einzelnen Komponenten. In the context of the invention, a bioreactor system or a photobioreactor system is understood to mean a system which is made up of various components, for example pipes, plates, foils or other components suitable for growing microorganisms. In addition, such a photobioreactor system includes pumps, inlet and outlet valves for nutrients, CO2, salts, water, etc. as well as fastening means for suspending or connecting the individual components.
Im Sinne der Erfindung wird unter einem Inkubationsbehälter ein Bauteil eines Bioreaktorsystems verstanden, welches hauptsächlich dazu dient, die Mikroorganismen zu vermehren und/oder zu kultivieren. Ein solcher Inkubationsbehälter kann verschiedene geometrische Formen aufweisen. Besonders vorteilhaft haben sich Röhren, Platten, Folien und/oder Schläuche erwiesen. Bei der Verwendung von phototrophen Mikroorganismen sind diese natürlich aus einem lichtdurchlässigen Material gefertigt, so dass die Mikroorganismen Photosynthese betreiben können. In the context of the invention, an incubation container is understood to mean a component of a bioreactor system which mainly serves to store the microorganisms to multiply and / or cultivate. Such an incubation container can have various geometric shapes. Tubes, plates, foils and / or hoses have proven to be particularly advantageous. When using phototrophic microorganisms, these are of course made of a translucent material so that the microorganisms can photosynthesize.
Vorteilhafterweise haben sich Bioreaktoren in der Bauweise als Röhren-Photobioreaktor, Tannenbaum-Photobioreaktor, Platten-Photobioreaktor oder Folien-Photobioreaktor erwiesen. Die vorliegende erfindungsgemäße Licht- und Temperaturvorrichtung lässt sich auf diese sowie auf alle gängigen Photobioreaktoren anwenden. Advantageously, bioreactors have proven to be constructed as a tube photobioreactor, Christmas tree photobioreactor, plate photobioreactor or film photobioreactor. The present light and temperature device according to the invention can be applied to this as well as to all common photobioreactors.
Gemäß einer vorteilhaften Ausführungsform der erfindungsgemäßen Licht- und Temperaturvorrichtung, ist diese in Form einer Manschette ausgebildet. Das heißt, die Licht- und Temperaturvorrichtung lässt sich um einen Inkubationsbehälter (z.B. Anordnung um die Röhrenlängen herum) herum anordnen oder installieren. Diese als Manschette ausgebildete Licht- und Temperaturvorrichtung, die mit LED-Lichtarrays bestückt ist, sorgt für eine bestmögliche Lichtzuführung für die Mikroorganismenkulturen. Gemäß einer weiteren vorteilhaften Ausführungsform der erfindungsgemäßen Licht- und Temperaturvorrichtung weist eine solche Manschette weitere Bauteile wie Verschalungs- oder Ummantelungsteile auf, um die einzelnen Komponenten der Manschette um einen Inkubationsbehälter z.B. eine Röhre anzuordnen und mittels geeigneter Befestigungsmittel (z.B. einer Klemme) zu fixieren. According to an advantageous embodiment of the light and temperature device according to the invention, it is designed in the form of a sleeve. That is, the light and temperature device can be arranged or installed around an incubation container (e.g. arrangement around the tube lengths). This light and temperature device, designed as a sleeve and equipped with LED light arrays, ensures the best possible light supply for the microorganism cultures. According to a further advantageous embodiment of the light and temperature device according to the invention, such a cuff has further components such as casing or sheathing parts in order to surround the individual components of the cuff around an incubation container, e.g. to arrange a tube and fix it with suitable fasteners (e.g. a clamp).
Gemäß einer weiteren vorteilhaften Ausführungsform der erfindungsgemäßen Licht- und Temperaturvorrichtung umfasst diese zumindest einen Kanal zum Anschluss von Kühlwasser oder Kühlflüssigkeit. Dabei durchzieht dieser Kanal die LED-Lichtanordnung und kann somit zur aktiven Kühlung der LEDs eingesetzt werden. Dies hat den Vorteil, dass zusätzlich zur passiven Kühlung mittels der Kühlrippen beispielsweise an heißen Tagen oder intensiverer Lichtbestrahlung eine aktive Kühlung betrieben werden kann, und somit ein optimales Temperaturniveau zur Vermehrung der Mikroorganismenkultur geschaffen werden kann. Ebenso kann durch geregelte Kühlmittel-Zirkulation in der LED-Lichtanordnung, ein gewünschter Temperatureintrag in dem Inkubationsbehälter (z.B. Röhrensystem) erzeugt werden, sodass ganzjährig und ortsunabhängig mit dem gleichen Klima kultiviert werden kann. Gemäß einer weiteren vorteilhaften Ausführungsform der Erfindung weist die Vorrichtung passende Anschlüsse für Zu- und Ableitung von Wasser oder Kühlmittel auf. Gemäß einer weiteren vorteilhaften Ausführungsform der erfindungsgemäßen Licht- und Temperaturvorrichtung weist diese mindestens eine elektrische Schnittstelle zum Anschluss von Mess- und/oder Überwachungsgeräten auf. Derartige Mess- und/oder Überwachungsgeräte können beispielsweise verschiedene Kameras, wie z.B. CCD- Kameras, Temperaturfühler, Spektrometer, Viskosimeter und Fluoreszenz- und Chlorophyll-Messgeräte, umfassen. According to a further advantageous embodiment of the light and temperature device according to the invention, it comprises at least one channel for connecting cooling water or cooling liquid. This channel runs through the LED light arrangement and can therefore be used for active cooling of the LEDs. This has the advantage that, in addition to passive cooling by means of the cooling fins, active cooling can be operated, for example, on hot days or more intensive light irradiation, and thus an optimal temperature level can be created for the multiplication of the microorganism culture. Likewise, through the regulated circulation of coolant in the LED light arrangement, a desired temperature input can be generated in the incubation container (e.g. tube system), so that the same climate can be cultivated all year round, regardless of location. According to a further advantageous embodiment of the invention, the device has suitable connections for the supply and discharge of water or coolant. According to a further advantageous embodiment of the light and temperature device according to the invention, it has at least one electrical interface for connecting measuring and / or monitoring devices. Such measuring and / or monitoring devices can include, for example, various cameras, such as CCD cameras, temperature sensors, spectrometers, viscometers and fluorescence and chlorophyll measuring devices.
Derartige Mess- und/oder Überwachungsgeräte können dabei zur Messung von Fluoreszenz, Lumineszenz, Strahlung, Fluidität, Viskosität, Farbumschlägen und/oder Chlorophyllgehalt ausgebildet sein. Such measuring and / or monitoring devices can be designed to measure fluorescence, luminescence, radiation, fluidity, viscosity, color changes and / or chlorophyll content.
Der Vorteil der Anbringung solcher Mess- und/oder Überwachungsgeräte an die erfindungsgemäße Licht- und/oder Temperaturvorrichtung besteht darin, dass diese dazu geeignet sind, beispielsweise den optimalen Lichteinsatz, den Erntezeitpunkt der Mikroorganismenkultur und/oder gegebenenfalls mögliche Kontaminationen der Kultur zu bestimmen oder zu erkennen. Ebenfalls kann beispielsweise mit einem Temperaturfühler die aktive Regulierung der Temperatur beispielsweise über Kühlwasser oder Kühlflüssigkeit, welche(s) dann in die Kanäle der LED-Lichtanordnung gepumpt werden kann, geregelt werden. The advantage of attaching such measuring and / or monitoring devices to the light and / or temperature device according to the invention is that they are suitable, for example, to determine or to determine the optimal use of light, the harvest time of the microorganism culture and / or possibly possible contamination of the culture detect. The active regulation of the temperature, for example via cooling water or cooling liquid, which can then be pumped into the channels of the LED light arrangement, can also be regulated, for example with a temperature sensor.
Gemäß einer weiteren Ausführungsform der erfindungsgemäßen Licht- und Temperaturvorrichtung weist diese zwischen und/oder seitlich der LED-Lichtanordnung und des Inkubationsbehälters eine Isolierung auf. Die Isolierung ist dabei derart ange bracht, dass die Lichteinstrahlung der LED-Lichtanordnung auf den Inkubationsbehälter hiervon nicht beeinflusst ist. Eine solche Isolierung umfasst beispielsweise Gummi, Kunststoff, Polyurethanen (PUR) oder Polyisocyanurat-Hartschaum (PIR). According to a further embodiment of the light and temperature device according to the invention, it has insulation between and / or to the side of the LED light arrangement and the incubation container. The insulation is applied in such a way that the light irradiation of the LED light arrangement on the incubation container is not influenced by this. Such insulation includes, for example, rubber, plastic, polyurethanes (PUR) or polyisocyanurate rigid foam (PIR).
Gemäß einer weiteren vorteilhaften Ausführungsform der erfindungsgemäßen Licht- und Temperaturvorrichtung kann auch das Befestigungsmittel eine Isolierung aufweisen oder eine spezielle Isolierung wird mittels eines Befestigungsmittels um den Inkubationsbehälter herum gehalten. Ebenfalls kann auch eine Isolierung den gesamten Inkubationsbehälter - mit Ausnahme der LED-Lichteinheit - gänzlich oder teilweise ummantelt oder abdecken. Die Isolierungsvarianten haben den Vorteil, dass beispielsweise der Inkubationsbehälter vor Beschädigung durch gegebenenfalls metallische oder harte Komponenten der erfindungsgemäßen Licht- und Temperaturvorrichtung nicht beschädigt oder zerkratzt wird. Ein weiterer Vorteil einer solchen Isolierung, beispielsweise wenn diese aus Gummi oder ähnlichem besteht, liegt darin, dass hierbei auch eine gewisse Rutschfestigkeit erreicht wird, was ein Verdrehen der erfindungsgemäßen Licht- und Temperaturvorrichtung insbesondere der LED- Lichtanordnung gegenüber dem Inkubationsbehälter verhindert. According to a further advantageous embodiment of the light and temperature device according to the invention, the fastening means can also have insulation or a special insulation is held around the incubation container by means of a fastening means. An insulation can also completely or partially encase or cover the entire incubation container - with the exception of the LED light unit. The insulation variants have the advantage that, for example, the incubation container is protected from damage by possibly metallic or hard components of the light and Temperature device is not damaged or scratched. Another advantage of such an insulation, for example if it is made of rubber or the like, is that it also achieves a certain slip resistance, which prevents twisting of the light and temperature device according to the invention, in particular the LED light arrangement, relative to the incubation container.
Gemäß einer weiteren Ausführungsform der erfindungsgemäßen Licht- und Temperaturvorrichtung kann diese eine oder mehrere LED-Lichtanordnungen aufweisen, welche kontinuierlich um eine Längsachse eines Inkubationsbehälters angeordnet sind. Bei einem vorwiegend kreisförmigen Querschnitt des Inkubationsbehälters können diese LED-Lichtanordnungen in Wickelabständen von 1 - 360 Grad um den Inkubationsbehäl ter angeordnet sein. According to a further embodiment of the light and temperature device according to the invention, it can have one or more LED light arrangements which are arranged continuously around a longitudinal axis of an incubation container. In the case of a predominantly circular cross section of the incubation container, these LED light arrangements can be arranged around the incubation container at winding intervals of 1 to 360 degrees.
Gemäß einer weiteren vorteilhaften Ausführungsform der erfindungsgemäßen Licht- und Temperaturvorrichtung sind die LED-Lichtanordnungen kontinuierlich um die Längsachse eines Inkubationsbehälters in Wnkelabständen von 180 Grad oder von 135 Grad oder bevorzugt von 90 Grad angeordnet. According to a further advantageous embodiment of the light and temperature device according to the invention, the LED light arrangements are arranged continuously around the longitudinal axis of an incubation container at angular intervals of 180 degrees or 135 degrees or preferably 90 degrees.
Gemäß einer weiteren vorteilhaften Ausführungsform der erfindungsgemäßen Licht- und Temperaturvorrichtung ist die LED-Lichtanordnung zur Emission eines fixen Lichtspektrums, auch bezeichnet als SMD-Ausführung (surface-mounted devices), oder zur Emission eines veränderbaren Lichtspektrums ausgebildet. Bei einem veränderbaren Lichtspektrum, welches auch als Multichannel-Ausführung bezeichnet wird, erhält jede Lichtfarbe einen separaten Kanal zum Ansteuern von 0 - 100% Lichtoutput. Das Spektrum kann somit dann variabel, bedarfsorientiert gesteuert werden. According to a further advantageous embodiment of the light and temperature device according to the invention, the LED light arrangement is designed to emit a fixed light spectrum, also referred to as an SMD version (surface-mounted devices), or to emit a variable light spectrum. With a changeable light spectrum, which is also referred to as a multichannel version, each light color receives a separate channel to control 0-100% light output. The spectrum can then be controlled variably, based on requirements.
Gemäß einer weiteren Ausführungsform der erfindungsgemäßen Licht- und Temperaturvorrichtung ist diese so konzipiert, dass zwischen der LED-Lichtanordnung bzw. den LED-Lichtarrays und dem Inkubationsbehälter der bautechnisch geringstmögliche Abstand vorliegt. Diese bautechnische Variante hat den Vorteil, dass der Energieeinsatz für die Belichtung der Mikroorganismenkultur hierdurch erheblich reduziert werden kann. According to a further embodiment of the light and temperature device according to the invention, it is designed in such a way that the smallest possible structural distance is between the LED light arrangement or the LED light arrays and the incubation container. This structural variant has the advantage that the energy input for the exposure of the microorganism culture can be reduced considerably.
Gemäß einer weiteren vorteilhaften Ausführungsform der erfindungsgemäßen Licht- und Temperaturvorrichtung ist diese zur wiederlösbaren Anbringung an einen röhrenförmigen, zylindrischen, quaderförmigen, prismenförmigen, pentaedrischen, hexaedrischen, heptaedrischen, oktaedrischen oder sonstig geometrisch gearteten länglichen Inkuba tionsbehälter ausgebildet. According to a further advantageous embodiment of the light and temperature device according to the invention, this is for detachable attachment to a tubular, cylindrical, cuboid, prism-shaped, pentahedral, hexahedral, heptahedral, octahedral or other geometrically shaped elongated incubation container formed.
Gemäß einer weiteren vorteilhaften Ausführungsform der erfindungsgemäßen Licht- und Temperaturvorrichtung ist diese aus Materialien, die für eine passive Kühlung geeignet sind, ausgebildet. Beispielsweise weisen Komponenten der Licht- und Temperaturvorrichtung Abschnitte aus Aluminium oder anderen zur Kühlung geeigneten Metallen oder Materialien auf. Insbesondere sind hierbei die Kühlrippen der LED- Lichtanordnung aus einem solchen Material gefertigt. According to a further advantageous embodiment of the light and temperature device according to the invention, it is made from materials that are suitable for passive cooling. For example, components of the light and temperature device have sections made of aluminum or other metals or materials suitable for cooling. In particular, the cooling fins of the LED light arrangement are made from such a material.
Nachfolgend wird die Erfindung anhand von spezifischen Ausführungsformen und Abbildungen näher beschrieben. Es zeigen: The invention is described in more detail below on the basis of specific embodiments and illustrations. Show it:
FIG. 1 eine schematische dreidimensionale Darstellung der erfindungsgemäßen Licht- und Temperaturvorrichtung gemäß einer vorteilhaften Ausführungsform im befestigten Zustand um einen Inkubationsbehälter; FIG. 1 a schematic three-dimensional representation of the light and temperature device according to the invention according to an advantageous embodiment in the attached state around an incubation container;
FIG. 2 eine schematische Darstellung eines Querschnitts der erfindungsgemäßen Licht- und Temperaturvorrichtung aus FIG. 1 ; FIG. 2 shows a schematic representation of a cross section of the light and temperature device according to the invention from FIG. 1 ;
FIG. 3 eine vergrößerte schematische Darstellung der LED-Lichtanordnung, welche am Inkubationsbehälter befestigt vorliegt gemäß dem Ausschnitt X aus FIG. 2. FIG. 3 shows an enlarged schematic representation of the LED light arrangement which is attached to the incubation container according to section X from FIG. 2.
FIG. 4 eine schematische Seitenansicht der erfindungsgemäßen Licht- und Tempera turvorrichtung gemäß FIG. 1 ; FIG. 4 is a schematic side view of the light and temperature device according to the invention according to FIG. 1 ;
FIG. 5 eine schematische Darstellung in der Aufsicht auf die erfindungsgemäße Licht- und Temperaturvorrichtung gemäß FIG. 1 ; FIG. 5 a schematic representation in a plan view of the light and temperature device according to the invention according to FIG. 1 ;
Nachfolgend wird die Erfindung anhand von bevorzugten beispielhaften Ausführungs formen und dazugehörigen Figuren beschrieben. Es wird angemerkt, dass die Figuren hierbei rein schematisch sind und keinerlei Rückschlüsse auf den exakten Maßstab zulassen. Die Figuren und die dazugehörige Beschreibung dienen lediglich dazu, das Prinzip der Erfindung zu verdeutlichen, jedoch schränken diese die Erfindung in keiner Weise ein. In FIG. 1 ist eine bevorzugte Ausführungsform der erfindungsgemäßen Licht- und Temperaturvorrichtung 10 in einer schematischen dreidimensionalen Darstellung abge bildet. Die erfindungsgemäße Licht- und Temperaturvorrichtung 10 befindet sich hierbei bereits im montierten Zustand an einem lichtundurchlässigen Inkubationsbehälter 40. Der Inkubationsbehälter 40 ist hierbei ein Plexiglasrohr, welches über Umlenkmodule mit anderen Plexiglasröhren zu einem sogenannten Röhren-Photobioreaktor verbaut werden kann. In diesen speziellen Ausführungsbeispielen weist die erfindungsgemäße Licht- und Temperaturvorrichtung 10 zwei gegenüberliegende LED-Lichtanordnungen 20 auf. Weiter ist in dieser Ausführung die erfindungsgemäße Licht- und Temperaturvorrichtung 10 in Form einer Manschette ausgebildet. Diese wird mittels Ummantelungskomponenten, in diesem Fall Viertelschalen 70, und hierfür vorgesehene Klemmen als Befestigungsmitteln um den Inkubationsbehälter herum ausgebildet. The invention will be described with reference to preferred exemplary embodiment forms and associated figures. It is noted that the figures here are purely schematic and do not allow any conclusions to be drawn about the exact scale. The figures and the associated description only serve to clarify the principle of the invention, but they do not restrict the invention in any way. In FIG. 1 is a preferred embodiment of the light and temperature device 10 according to the invention in a schematic three-dimensional representation. The light and temperature device 10 according to the invention is already in the assembled state on an opaque incubation container 40. The incubation container 40 is a plexiglass tube, which can be built into a so-called tube photobioreactor via deflection modules with other plexiglass tubes. In these special exemplary embodiments, the light and temperature device 10 according to the invention has two opposing LED light arrangements 20. Furthermore, in this embodiment, the light and temperature device 10 according to the invention is designed in the form of a sleeve. This is formed around the incubation container by means of sheathing components, in this case quarter shells 70, and clamps provided for this purpose as fastening means.
FIG. 2 zeigt die Ausführungsform aus FIG. 1 in einem schematischen Querschnitt. Zu sehen sind die beiden LED-Lichtanordnungen 20 sowie die Viertelschalen 70. Durch die Viertelschallen 70 wird außerdem ein Isolationsmaterial 60 um den Inkubationsbehälter 40 mittels der Klemmen 30 befestigt. FIG. 2 shows the embodiment from FIG. 1 in a schematic cross section. The two LED light arrangements 20 as well as the quarter shells 70 can be seen. The quarter shells 70 also fasten an insulation material 60 around the incubation container 40 by means of the clamps 30.
FIG. 3 zeigt in einen vergrößerten Bereich einen schematischen Querschnitt der LED- Lichtanordnung 20 aus FIG. 2. Hierbei ist die LED-Lichtanordnung 20 zu sehen, welche über Kühlrippen 22 sowie LED-Lichtarrays 21 verfügt. Weiter ist ein Anschluss für Wasser oder Kühlflüssigkeit sichtbar, welcher weiter in einen Kanal 50 mündet, der sich durch die LED-Lichtanordnung 20, in diesem Fall durch das Kühlrippenprofil 22, zieht. Ebenfalls zu sehen ist eine spezielle Klemme 30 zur Halterung des Kühlprofiles 22 am Inkubationsbehälter 40. FIG. 3 shows, in an enlarged area, a schematic cross section of the LED light arrangement 20 from FIG. 2. The LED light arrangement 20 can be seen here, which has cooling fins 22 and LED light arrays 21. A connection for water or cooling liquid can also be seen, which further opens into a channel 50 which runs through the LED light arrangement 20, in this case through the cooling rib profile 22. A special clamp 30 for holding the cooling profile 22 on the incubation container 40 can also be seen.
Die spezielle Ausführungsform der erfindungsgemäßen Licht- und Temperaturvorrich tung 10 gemäß FIG. 1 ist in FIG. 5 in der Aufsicht sowie in FIG. 4 in Seitenansicht zu sehen. The special embodiment of the invention Licht- und Temperaturvorrich device 10 according to FIG. 1 is shown in FIG. 5 in plan view and in FIG. 4 can be seen in side view.
Diese in den Figuren 1 - 5 beschriebene spezielle Ausführungsform der erfindungsge mäßen Licht- und Temperaturvorrichtung 10 ist besonders geeignet für die Konzeption in Röhren-Photobioreaktoren. Als Induktionsbehälter 40 kommen hierfür zum Beispiel Plexiglasröhren infrage. In der speziellen Ausführungsform sind diese Röhren 40 mit der erfindungsgemäßen Licht- und Temperaturvorrichtung 10 versehen, welche die Form einer Manschette bildet. Diese Ausführungsform wird auch als Lichtmanschette bezeichnet. Die Lichtmanschetten können dabei um die Röhren herum gelegt werden. Wobei diese mittels Viertelschalen 70 und speziellen Klemmen 30 wieder lösbar angebracht werden. Die Lichtmanschetten beinhalten dabei LED-Lichtanordnungen 20, die direkt, einseitig oder wie in diesem Fall zweiseitig um 180 Grad versetzt Licht in die Röhren einkoppeln können. Die Lichtmanschetten sind dabei bevorzugt aus Aluminium oder anderen, für Kühlung geeigneten Materialien gefertigt. Die LED-Lichtanordnungen 20 in diesem Ausführungsbeispiel verfügen über Kühlrippen 22 auf der Außenseite der Lichtanordnung 20, um die entstehende Abwärme der LEDs nach außen abführen zu können. Zudem verfügen die LED-Lichtanordnungen 20 über Kühlkanäle 50 mit Anschlüssen, in die Kühlflüssigkeit oder Wasser eingebracht werden kann. Die Temperatur der Kühlflüssigkeit kann über eine Verbindung zu Kälteanlagen, Wärmetauschern oder ähnlichem geregelt werden. Dies ermöglicht einstellbare und geregelte Temperaturen an den Röhren, was eine positive Wirkung auf die Produktion der Mikroorganismenkultur hat. Die mit LED-Lichtarrays 21 bestückten Lichtanordnungen 20 sorgen für eine bestmögliche Lichtzuführung für die Mikroorganismenkulturen. Die LED-Lichtanordnung 20 kann dabei als SMD-Ausführung mit einem fixen Lichtspektrum oder als Multichannel-Ausführung, wobei jede Lichtfarbe einen separaten Kanal zum Ansteuern von 0 - 100% Lichtoutput erhält, ausgestaltet sein. Ein solches Spektrum kann dann variabel, bedarfsorientiert gesteuert werden. Der Energieeinsatz kann dadurch reduziert werden, dass die LED-Lichtquelle möglichst ohne Abstand an die Röhre herangebracht wird. Weiter kann der Energieeinsatz reduziert werden, wenn das spezielle Lichtspektrum auf die jeweils zu kultivierenden Mikroorganismen abgestellt wird. Mit einer in dieser speziellen Ausführungsform beschriebenen Anordnung gibt es auch keine Eigenverschattung durch die Röhrenanordnung selbst. Die Lichtmanschetten können hierbei in verschiedenen Größen für verschiedene Röhrendurchmesser zum Einsatz kommen. Die LED- Lichtquellen sind perfekt auf das Photosynthesespektrum der Mikroorganismenkulturen abstimmbar, was beispielsweise unter Zuhilfenahme von verschiedenen Mess- und/oder Überwachungsgeräten realisiert werden kann. Um solche Mess- und/oder Überwachungsgeräte an die Lichtmanschetten anschließen zu können, verfügen diese vorteilhafterweise über mindestens eine elektronische Schnittstelle. So kann beispielsweise mittels einer Mini-Kamera eine Fluoreszenzmessung vorgenommen werden, die dazu genutzt werden kann, den optimalen Lichteinsatz, den Erntezeitpunkt und andere Parameter optimal zu bestimmen. Auch kann mit einem Temperaturfühler beispielsweise die optimale Temperatur für die Mikroorganismenkultur gemessen werden und bei Bedarf aktiv über die Kühlkanäle der LED-Lichtanordnung angepasst werden. This special embodiment of the light and temperature device 10 according to the invention, described in FIGS. 1-5, is particularly suitable for the design in tube photobioreactors. Plexiglass tubes, for example, can be used as induction containers 40 for this purpose. In the special embodiment, these tubes 40 are provided with the light and temperature device 10 according to the invention, which forms the shape of a sleeve. This embodiment is also called a light cuff designated. The light cuffs can be placed around the tubes. These are releasably attached again by means of quarter shells 70 and special clamps 30. The light sleeves contain LED light arrangements 20, which can couple light into the tubes directly, on one side or, as in this case, on both sides offset by 180 degrees. The light sleeves are preferably made of aluminum or other materials suitable for cooling. The LED light arrangements 20 in this exemplary embodiment have cooling ribs 22 on the outside of the light arrangement 20 in order to be able to dissipate the resulting waste heat from the LEDs to the outside. In addition, the LED light arrangements 20 have cooling channels 50 with connections into which cooling liquid or water can be introduced. The temperature of the cooling liquid can be regulated via a connection to refrigeration systems, heat exchangers or the like. This enables adjustable and controlled temperatures on the tubes, which has a positive effect on the production of the microorganism culture. The light arrangements 20 equipped with LED light arrays 21 ensure the best possible light supply for the microorganism cultures. The LED light arrangement 20 can be designed as an SMD version with a fixed light spectrum or as a multichannel version, with each light color receiving a separate channel for controlling 0-100% light output. Such a spectrum can then be controlled variably, based on requirements. The energy consumption can be reduced by bringing the LED light source as close as possible to the tube without any distance. The use of energy can also be reduced if the special light spectrum is tailored to the microorganisms to be cultivated. With an arrangement described in this specific embodiment, there is also no inherent shading by the tube arrangement itself. The light sleeves can be used in different sizes for different tube diameters. The LED light sources can be perfectly matched to the photosynthesis spectrum of the microorganism cultures, which can be achieved, for example, with the aid of various measuring and / or monitoring devices. In order to be able to connect such measuring and / or monitoring devices to the light sleeves, they advantageously have at least one electronic interface. For example, a mini camera can be used to measure fluorescence, which can be used to optimally determine the optimal use of light, the time of harvest and other parameters. A temperature sensor can also be used, for example, to measure the optimum temperature for the microorganism culture and, if necessary, can be actively adapted to the LED light arrangement via the cooling channels.
Bezugszeichenliste List of reference symbols
10 Licht- und Temperaturvorrichtung 20 LED-Lichtanordnung 10 Light and temperature device 20 LED light arrangement
21 LED-Lichtarrays 21 LED light arrays
22 Kühlrippen 22 cooling fins
30 Befestigungsmittel 30 fasteners
40 Inkubationsbehälter 40 incubation containers
50 Kanal 50 channel
60 Isolierung 60 insulation
70 Verschalung 70 casing

Claims

Patentansprüche Claims
1. Licht- und Temperaturvorrichtung (10) zur Erhöhung und Optimierung der Produktion von Biomasse in Bioreaktoren, umfassend 1. Light and temperature device (10) for increasing and optimizing the production of biomass in bioreactors, comprising
eine LED-Lichtanordnung (20), wobei diese LED-Lichtarrays (21) und Kühlrippen (22), welche von den LED-Lichtarrays (21) wegzeigen, aufweist; und an LED light assembly (20), said LED light arrays (21) and cooling fins (22) facing away from the LED light arrays (21); and
mindestens ein Befestigungsmittel (30) zur wiederlösbaren Anbringung der Lichtanordnung (20) an einen lichtdurchlässigen Inkubationsbehälter (40), wobei die LED-Lichtanordnung 20 derart zum Inkubationsbehälter (40) ausgerichtet ist, dass eine von der LED-Lichtanordnung 20 ausgehende Lichtstrahlung zum Inkubationsbehälter (40) gerichtet ist. at least one fastening means (30) for the releasable attachment of the light arrangement (20) to a translucent incubation container (40), the LED light arrangement 20 being aligned with the incubation container (40) in such a way that a light radiation emanating from the LED light arrangement 20 to the incubation container ( 40) is directed.
2. Licht- und Temperaturvorrichtung (10) nach Anspruch 1 , dadurch gekennzeichnet, dass die Licht- und Temperaturvorrichtung (10) in Form einer Manschette ausge bildet ist, wobei diese eine Verschalung (70) umfasst, die mittels des Befestigungsmittels (30) um den Inkubationsbehälter (40) herum wieder lösbar angeordnet ist. 2. light and temperature device (10) according to claim 1, characterized in that the light and temperature device (10) is formed in the form of a sleeve, which comprises a casing (70) which by means of the fastening means (30) around the incubation container (40) is detachably arranged around it again.
3. Licht- und Temperaturvorrichtung (10) nach einem der vorhergehenden Ansprüche, dadurch gekennzeichnet, dass die Licht- und Temperaturvorrichtung (10) mindestens einen Kanal (50) für Kühlwasser oder Kühlflüssigkeit umfasst, wobei der Kanal (50) in der LED-Lichtanordnung (20) angeordnet ist. 3. light and temperature device (10) according to any one of the preceding claims, characterized in that the light and temperature device (10) comprises at least one channel (50) for cooling water or cooling liquid, wherein the channel (50) in the LED light arrangement (20) is arranged.
4. Licht- und Temperaturvorrichtung (10) nach einem der vorhergehenden Ansprüche, dadurch gekennzeichnet, dass die Licht- und Temperaturvorrichtung (10) mindestens eine elektrische Schnittstelle zum Anschluss von Mess- und/oder Überwachungsgeräten umfasst. 4. light and temperature device (10) according to any one of the preceding claims, characterized in that the light and temperature device (10) comprises at least one electrical interface for connecting measuring and / or monitoring devices.
5. Licht- und Temperaturvorrichtung (10) nach Anspruch 4, dadurch gekennzeichnet, dass die Mess- und/oder Überwachungsgeräte Kameras, CCD-Kameras, Temperaturfühler, Spektrometer, Viskosimeter, Fluoreszenz-/Lumineszenz-Messgeräte und/oder Chlorophyll-Messgeräte umfassen, wobei diese zur Messung von Fluoreszenz, Lumineszenz, Strahlung, Fluidität, Farbumschlägen und/oder Chlorophyllgehalt ausgebildet sind. 5. Light and temperature device (10) according to claim 4, characterized in that the measuring and / or monitoring devices include cameras, CCD cameras, temperature sensors, spectrometers, viscometers, fluorescence / luminescence measuring devices and / or chlorophyll measuring devices, these being designed to measure fluorescence, luminescence, radiation, fluidity, color changes and / or chlorophyll content.
6. Licht- und Temperaturvorrichtung (10) nach einem der vorhergehenden Ansprüche, dadurch gekennzeichnet, dass zwischen und/oder seitlich der LED- Lichtanordnung (20) und des Inkubationsbehälters (40) eine Isolierung (60) derart angebracht ist, dass die Lichteinstrahlung der LED-Lichtanordnung (20) auf den Inkubationsbehälter (40) hiervon nicht beeinflusst ist und/oder dass das Befestigungsmittel (30) eine Isolierung (60) aufweist und/oder dass eine Isolierung (60) den gesamten Inkubationsbehälter (40) - mit Ausnahme der LED-Lichteinheit (20) - ummantelt. 6. light and temperature device (10) according to any one of the preceding claims, characterized in that between and / or side of the LED light arrangement (20) and the incubation container (40) an insulation (60) is attached such that the light radiation of the LED light arrangement (20) on the incubation container (40) is not influenced by this and / or that the fastening means (30) has an insulation (60) and / or that an insulation (60) covers the entire incubation container (40) - with the exception of the LED light unit (20) - encased.
7. Licht- und Temperaturvorrichtung (10) nach einem der vorhergehenden Ansprüche, dadurch gekennzeichnet, dass eine oder mehrere LED-Lichtanord- nungen (20) um eine Längsachse des Inkubationsbehälters (40) in Winkelschritten von 1 Grad bis 360 Grad angeordnet sind, oder dass die LED-Lichtanordnungen (20) um die Längsachse des Inkubationsbehälters (40) in Winkelschritten von 90 Grad oder von 135 Grad oder von 180 Grad angeordnet sind. 7. light and temperature device (10) according to one of the preceding claims, characterized in that one or more LED light arrangements (20) are arranged around a longitudinal axis of the incubation container (40) in angular steps of 1 degree to 360 degrees, or that the LED light arrangements (20) are arranged around the longitudinal axis of the incubation container (40) in angular steps of 90 degrees or of 135 degrees or of 180 degrees.
8. Licht- und Temperaturvorrichtung (10) nach einem der vorhergehenden Ansprüche, dadurch gekennzeichnet, dass die LED-Lichtanordnung (20) zur Emission eines fixen Lichtspektrums oder zur Emission eines veränderbaren Lichtspektrums ausgebildet ist, wobei jede Lichtfarbe einen separaten Kanal zum Ansteuern von 0 - 100% Lichtoutput aufweist. 8. light and temperature device (10) according to any one of the preceding claims, characterized in that the LED light arrangement (20) is designed to emit a fixed light spectrum or to emit a variable light spectrum, each light color having a separate channel for controlling 0 - Has 100% light output.
9. Licht- und Temperaturvorrichtung (10) nach einem der vorhergehenden Ansprüche, dadurch gekennzeichnet, dass diese zur wiederlösbaren Anbringung an einen röhrenförmigen, zylindrischen, quaderförmigen, prismenförmigen, pentae- drischen, hexaedrischen, heptaedrischen, oktaedrischen oder sonstig gearteten geometrisch Inkubationsbehälter (40) ausgebildet ist. 9. Light and temperature device (10) according to one of the preceding claims, characterized in that it is for releasable attachment to a tubular, cylindrical, cuboid, prism-shaped, pentahedral, hexahedral, heptahedral, octahedral or other type of geometric incubation container (40) is trained.
10. Licht- und Temperaturvorrichtung (10) nach einem der vorhergehenden Ansprüche, dadurch gekennzeichnet, dass das Befestigungsmittel (30) eine Klemme ist. 10. Light and temperature device (10) according to one of the preceding claims, characterized in that the fastening means (30) is a clamp.
EP20724429.4A 2019-04-29 2020-04-28 Light and temperature device for optimizing the production of biomass in bioreactors Pending EP3963042A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102019110989.2A DE102019110989A1 (en) 2019-04-29 2019-04-29 Light and temperature device for optimizing the production of biomass in bioreactors
PCT/EP2020/061787 WO2020221761A1 (en) 2019-04-29 2020-04-28 Light and temperature device for optimizing the production of biomass in bioreactors

Publications (1)

Publication Number Publication Date
EP3963042A1 true EP3963042A1 (en) 2022-03-09

Family

ID=70613733

Family Applications (1)

Application Number Title Priority Date Filing Date
EP20724429.4A Pending EP3963042A1 (en) 2019-04-29 2020-04-28 Light and temperature device for optimizing the production of biomass in bioreactors

Country Status (4)

Country Link
US (1) US20220204897A1 (en)
EP (1) EP3963042A1 (en)
DE (1) DE102019110989A1 (en)
WO (1) WO2020221761A1 (en)

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7220018B2 (en) * 2003-12-15 2007-05-22 Orbital Technologies, Inc. Marine LED lighting system and method
DE202006003878U1 (en) * 2005-03-16 2006-06-14 Sartorius Ag Lighting device for cultivating phototropic cell cultures in incubators, comprises controllable light emitting diodes arranged on light elements, which forms a lighting matrix
JP2008237067A (en) * 2007-03-26 2008-10-09 Ccs Inc Method for culturing algae
DE202007013406U1 (en) * 2007-09-25 2007-11-22 Lehr, Florian, Dipl.-Ing. Lighting device for bioreactors
CA2724794C (en) * 2008-05-22 2015-09-08 Syddansk Universitet Method and apparatus for using light emitting diodes in a greenhouse setting
KR20160081301A (en) * 2014-12-31 2016-07-08 (주)웰니스앤 Algae culture medium system using controlling LED lamp position
DE102017008769B4 (en) * 2017-09-19 2022-03-10 Sartorius Stedim Biotech Gmbh Illumination for a disposable photobioreactor

Also Published As

Publication number Publication date
US20220204897A1 (en) 2022-06-30
DE102019110989A1 (en) 2020-10-29
WO2020221761A1 (en) 2020-11-05

Similar Documents

Publication Publication Date Title
EP2417243B1 (en) Photobioreactor and method for cultivating biomass by means of photosynthesis
US20060035370A1 (en) Multi-layered photobioreactor and method of culturing photosynthetic microorganisms using the same
EP3041924B1 (en) Device for obtaining phytoplankton (microalgae)
US20100170149A1 (en) Algae production systems and associated methods
WO2005079560A1 (en) Device for cultivating and mass-producing algae
DE102009028474A1 (en) Device and method for the production of biomass
CA2581100A1 (en) Method for raising aquatic animals
CN105393973A (en) Fish and vegetable co-existing water circulation system
CN102162308A (en) Indoor three-dimensional ecological planting and breeding system
DE102011002763A1 (en) Photobioreactor with illumination by means of light fittings
WO2015106733A9 (en) Photobioreactor and method for thin layer cultivation
EP2289308A3 (en) Breeding plants
EP3963042A1 (en) Light and temperature device for optimizing the production of biomass in bioreactors
DE102009021059A1 (en) System for microalgae production, comprises a lateral rectangular basin having a removable flat cover, an automatic lighting- and cleaning device, and nutrient- and carbon dioxide supply over a feed system admitted in a basin bottom side
EP3377604B1 (en) System for growing and reproducing microorganisms
DE102012013587A1 (en) Bioreactor designed as a photo-bioreactor comprises a disposable bag, which has a reactor chamber bounded by a wall, and light sources arranged in the immediate vicinity of the wall of the disposable bags
TWM562004U (en) Coral aquaculture system in production
WO2018115092A1 (en) Agricultural unit, agricultural module, agricultural system and method for operating an agricultural system
CN208143743U (en) A kind of agricultural planting device of ploughing
CN207227404U (en) A kind of biological inoculum equipment for breeding
CH710738A2 (en) Method and system for utilizing the waste heat of a data processing system.
CN206390033U (en) Aquatile visualizes circulation
DE102008031769A1 (en) Photobioreactor in flat design
CN105052808A (en) Whole ecological aquaculture system
DE202017107091U1 (en) Algae culturing device

Legal Events

Date Code Title Description
STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: UNKNOWN

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE

PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE

17P Request for examination filed

Effective date: 20211125

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

DAV Request for validation of the european patent (deleted)
DAX Request for extension of the european patent (deleted)