EP3817859A1 - Dispositif de capture de particules par diélectrophorèse - Google Patents

Dispositif de capture de particules par diélectrophorèse

Info

Publication number
EP3817859A1
EP3817859A1 EP19735269.3A EP19735269A EP3817859A1 EP 3817859 A1 EP3817859 A1 EP 3817859A1 EP 19735269 A EP19735269 A EP 19735269A EP 3817859 A1 EP3817859 A1 EP 3817859A1
Authority
EP
European Patent Office
Prior art keywords
layer
layers
obstacle
particles
obstacle structure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP19735269.3A
Other languages
German (de)
English (en)
Inventor
Samir KADIC
Christoph FAIGLE
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Robert Bosch GmbH
Original Assignee
Robert Bosch GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Robert Bosch GmbH filed Critical Robert Bosch GmbH
Publication of EP3817859A1 publication Critical patent/EP3817859A1/fr
Pending legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/02—Separators
    • B03C5/022—Non-uniform field separators
    • B03C5/026—Non-uniform field separators using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/005—Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0424—Dielectrophoretic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00—Details of magnetic or electrostatic separation
    • B03C2201/26—Details of magnetic or electrostatic separation for use in medical applications

Definitions

  • the invention relates to a device for dielectrophoretic capture of particles and a method for producing a corresponding device.
  • Circulating tumor cells CTC ' s
  • cell-free tumor DNA Circulating Tumor DNA, ctDNA
  • dielectrophoretic capture of particles at least comprising one or more layers and electrical contacting, the layers each having a top layer, a bottom layer and an obstacle structure.
  • a fluid comprising the particles can flow through the obstacle structure.
  • the obstacle structure is arranged on the top side of the layer, the obstacle structure spacing the top side of the layer from the bottom side of the same layer or a further one of the layers.
  • the solution proposed here is based in particular on the approach of manipulating particles according to their dielectric properties. This approach has the particular advantages that it can be scaled independently of markers and on a large scale, as well as being simple, non-contact, versatile, and very easy to integrate into modern MEMS and microfluidic technologies.
  • a particularly preferred component of the solution proposed here is the superimposition of a layer that can be caught by D EP force, in particular
  • the at least one layer is also referred to below as the so-called “DEP band”.
  • the device is used for (targeted) dielectrophoretic capture of
  • the device serves for the targeted dielectrophoretic capture of particles of a certain type or type (target cells) or
  • the solution presented here advantageously allows at least one specific type of particle to be specifically captured from a medium guided in the channel and thus to be made removable or removable.
  • the type of particle or type of particle is determined in particular by its dielectric properties.
  • tumor cells circulating in the blood differ in their dielectric properties.
  • the particles to be captured are in particular circulating
  • Each cell usually has its own unique morphology. Among other things, it is a function of the cell type, the complexity of the cell interior and the phase in the cell cycle. The cell membrane of most cell types is still not smooth, but is actually littered with wrinkles and microvilli.
  • tumor cells form a solid tissue from which in the course of tumor growth single circulating tumor cells (so-called. CTC's) can be detached. Because of the increased packing density in the original tissue, their actual cell membrane area is therefore larger than that of the healthy blood cells freely present in the blood and increases with increasing disorder due to the progressive growth of the tumor.
  • CTC's tumor growth single circulating tumor cells
  • the cell membrane area is the morphological property that is evident in the different transition frequencies of the two cell types:
  • the transition frequencies for five types of leukemia cells were between 60 kHz and 100 kHz.
  • Crossover frequencies of the most abundant subpopulations of healthy blood cells - namely lymphocytes and granulocytes - have very low standard deviations and are at least 5 to 7 standard deviations away from the crossover frequencies of most tumor types Dielectrophoresis suitable for the reliable isolation of all types of solid tumors and - albeit with lower efficiencies - applicable for (highly concentrated) leukemia populations.
  • Particle types Especially when the operating frequency lies between the respective transition frequencies, tumor cells can be attracted by pDEP and healthy blood cells can be rejected by nDEP.
  • the obstacle structure and / or the electrical contacting is or are in particular set up such that corresponding operating frequencies can be set here.
  • dielectrophoresis is at the basis of dielectrophoretic capture. This describes the movement of (also uncharged) polarizable particles in a non-homogeneous electric field. A dipole induced in the particle as a result of an external electrical alternating field interacts with this external field and leads to a dielectrophoretic force effect on the particle. This force effect can be used here to hold back the particle and thus its dielectrophoretic capture.
  • Constriction instead, in which particles can be captured using pDEP.
  • a DC voltage can often also cause a flow of the fluid at the same time.
  • the field is usually generated by directly applying an electrical voltage to obstacle structures (Electrode structures), especially metal (obstacle) structures
  • the device further comprises one or more layers.
  • the device comprises exactly or only one layer, for example if the layer is e.g. B. is wound into a so-called winding cylinder.
  • the device can have a plurality of layers, for example if the layers are stacked one above the other to form a stack.
  • the layers preferably each comprise at least one (electrically insulating) insulator layer or insulation layer.
  • the layers can each comprise at least one (flat) layer which is set up in such a way that it has an electrically insulating effect.
  • This insulator layer can be formed, for example, with a film.
  • the (insulator) films can be, for example, (thin, spin-coated) polyimide films, in particular with a thickness of, for example, up to 25 ⁇ m.
  • the device comprises an electrical contact.
  • the electrical contacting is in particular set up to form an electrical field within the fluid channel and in particular in the area of the obstacle structures.
  • the electrical contact can generally be connected or connected to a voltage supply.
  • the contacting can be two, for example
  • the contact arms preferably extend on the top side of the layer and / or in the region of the obstacle structure (formed as an isolator structure for iDEP applications). Furthermore, contact arms can preferably be formed both on the top side of the layer and on the underside of the layer. The contact arms are preferably in particular on the top of the layer and / or
  • Layer underside (flat) conductor tracks formed.
  • the contact arms form a positive pole and the other contact arm form a negative pole.
  • a particularly inhomogeneous electric field can thus be set between the contact arms, the field lines of which can be curved or deflected by the obstacle structure (insulator structure).
  • the contacting can be set up, for example, to form the electrodes of the electrodes
  • the contact can have contact arms which, for example, connect some of the electrodes of the electrode structure to a positive pole and others of the electrodes of the electrode structure to a negative pole. A particularly inhomogeneous electric field can thus be set between the electrodes of the electrode structure.
  • the contacting can connect the electrode extending along and / or in position, for example with a positive pole or negative pole.
  • the layers each have a top side and a bottom side.
  • the layers are preferably each flat. As a rule, these each have a flat top side and a flat bottom side. If several layers are provided, these are preferably formed identically.
  • the layers can each be constructed with a plurality of layers arranged one above the other and / or flat.
  • the layers each have an obstacle structure.
  • a fluid comprising the particles can flow through the obstacle structure.
  • the fluid is usually blood. That is further
  • Obstacle structure arranged on top of the layer.
  • an obstacle structure is arranged on the top of the layer and an obstacle structure on the underside of the layer.
  • the obstacle structure is configured to face the top of the layer from the underside of the same layer (for example, if only one layer is provided that is wound into a cylinder) or another of the layers (for example if there are several layers that are stacked into a stack) spacing.
  • the obstacle structures extend in particular along at least one (longitudinal) section of the position and / or (in a developed state of the Location) on one level. At least one of the obstacle structures preferably has a plurality of (electrically insulating and / or electrically insulated or in the form of rod electrodes) posts. Each obstacle structure particularly preferably has a plurality of posts. An obstacle structure can, for example, run along one
  • Longitudinal section of the layer extend that in the longitudinal direction of the layer (which may relate to a development direction of the layer) several posts of the
  • Obstacle structure are arranged side by side.
  • multiple posts of the obstacle structure transverse to the longitudinal direction
  • the obstacle structures are preferably each set up in such a way that they set a (certain, in particular predefined) spatial one
  • the electrical field is formed in particular within the fluid channel and in particular in the area of the obstacle structures (possibly (in the case of mDEP) even from the obstacle structures).
  • the obstacle structures are particularly preferably set up to form particle-type-specific energetic minima in the fluid channel. The meaning of the term “energetic minima” is explained in more detail below. This advantageously allows certain (or only) certain particles or particles of a certain type to be captured in the fluid channel. In this
  • the obstacle structures are dimensioned specifically for the particle type. In other words, this means in particular that the obstacle structures are dimensioned according to the partial chart (target cell) that is to be captured.
  • At least one of the layers is wound.
  • this layer is also preferably wound into a so-called winding cylinder.
  • the layer is particularly preferably wound spirally.
  • the layer is wound in such a way that a (microfluidic) fluid channel is formed between the top side and the bottom side of the same layer, in which the
  • Obstacle structure is arranged.
  • several of the layers are stacked.
  • These layers each have an obstacle structure.
  • at least one (smooth) layer can be provided in the stack or stack, which has no obstacle structure, for example as
  • a (microfluidic) fluid channel is formed, in which one of the obstacle structures is arranged.
  • Obstacle structure is an isolator structure.
  • the obstacle structures are formed with or from an electrically insulating material.
  • Such obstacle structures are used in particular when implementing an iDEP system (insulator based dielectrophoresis, iDEP).
  • the insulating material protrudes into the channel in the manner of posts, possibly even spanning at least part of a channel cross section.
  • Obstacle structure is an electrode structure.
  • the obstacle structures are formed with or from an electrically conductive material.
  • Such obstacle structures are used in particular when implementing an mDEP system (metal based dielectrophoresis, mDEP).
  • the electrically conductive material protrudes into the channel in the manner of posts in particular, possibly even spanning at least part of a channel cross section.
  • the electrode structures are each formed with a large number of microelectrodes. Those are preferred
  • the obstacle structures include both cathodes and anodes. At least one of the obstacle structures particularly preferably comprises the same number of cathodes as anodes.
  • the (necessary) (micro) electrodes could e.g. B. structured with lithographic methods directly on the top of the layer.
  • thermally vapor-deposited or galvanically applied metal such as gold or copper, is suitable for this.
  • the device further comprises at least one electrical passivation or electrical insulation.
  • the layer preferably comprises electrical passivation, in particular over the entire surface, preferably on the top side of the layer.
  • the electrical passivation can also cover at least part of the surface of the obstacle structure that is arranged on the layer.
  • the electrical passivation can be formed, for example, with a chemically inert but electrically transparent material.
  • the electrical insulation can be formed, for example, with one or more insulator layers of one layer.
  • the device further comprises at least one electrode that extends at least partially along (and / or in) one of the layers.
  • the electrode preferably extends within the (flat) material of the layer, which can be achieved, for example, by arranging the electrode between two one above the other
  • the layer is preferably formed with a sandwich arrangement of two insulator layers and a metal electrode embedded therein over the entire surface.
  • a method for producing a device proposed here is also proposed, at least comprising one
  • FIG. 3 a position according to FIG. 1 or from FIG. 2 in a perspective
  • Fig. 7 an illustration of a step of the proposed here
  • Device for dielectrophoretic capture of particles can be:
  • the underlying mechanism, the so-called dielectrophoresis (DEP) describes the movement (also uncharged) of polarizable particles in a non-homogeneous electric field.
  • DEP dielectrophoresis
  • a dipole induced in the particle as a result of an external electrical alternating field interacts with this external field and leads to a dielectrophoretic
  • the time-average dielectrophoretic force on a particle can in the most general case be used for a spatially stationary E- Field as
  • E RMS is the effective value of the E field vector (root mean square
  • the last factor ⁇ E RMS ⁇ in the above expression for (F DEP ) is particularly important, which occurs regardless of the material, shape and size of the target particle. In addition to the amplitude and the temporal distribution of the E field, it expresses its spatial inhomogeneity. This spatial
  • Inhomogeneity in a microfluidic channel can be achieved, for example, by suitable structuring of microelectrodes in the channel and direct application of an appropriate electrical signal to them (metal based
  • mDEP dielectrophoresis
  • Isolator structures in the channel and outside of the created E-field
  • iDEP insulator based dielectrophoresis
  • both variants set themselves the goal, by appropriate dimensioning of V
  • a 10 ml blood sample would have to be taken in a channel with a height of 50 pm within one hour
  • an object of the invention is, in particular, to achieve a
  • dielectrophoretic particle capture at a sufficiently high throughput in would take up a volume that is as manageable as possible
  • the expansion of a quasi-planar DEP structure can be expanded by a third spatial dimension, so that compression flows through as large as possible
  • Cross-sectional area can be made available.
  • the arrangement is in particular able to keep the target cells as small as possible
  • Fig. 1 shows schematically a layer 4 for a device proposed here in a sectional view.
  • the layer 4 has a layer top 6, one
  • the obstacle structure 8 is made of a fluid comprising the particles 2, 3 (not shown here)
  • the obstacle structure 8 is arranged on the top side 6 of the layer.
  • the layer 4 is formed, for example, in the manner of a DEP film.
  • the layer 4 has a sandwich arrangement made up of two insulator layers 13 and one which is embedded over the entire surface thereof
  • the insulator layers 13 represent an example of how the layer 4 can have electrical insulation 11.
  • Counter electrodes form extruded metal posts, which are applied to one of the two insulator layers 13 and are connected to one another on the bottom by flat conductor tracks 14 (not shown here, see FIG. 3) (for example, there is a change of polarities in the checkerboard pattern between the posts) ,
  • the extruded metal posts represent an example of how the obstacle structure 8 can be designed as an electrode structure.
  • the layer 4, as shown in FIG. 1 has, for example, a full-area electrical passivation 10 of the strip.
  • This electrical passivation 10 can be formed, for example, with a chemically inert, but electrically as transparent as possible material.
  • the layer 4 in FIG. 1 has, for example, a thin adhesive layer 15 on the back of the tape or on the bottom 7 of the layer. This adhesive layer 15 could stacking for ultimate strength and later in operation for tightness of the microchannels.
  • FIG. 2 schematically shows a device 1 proposed here in a sectional illustration.
  • the reference symbols are used uniformly, so that reference can be made in full to the above explanations relating to FIG. 1.
  • the device 1 is set up for the dielectrophoretic capture of particles 2, 3 (not shown here).
  • the device 1 comprises one or more layers 4 and an electrical contact 5 (not shown here).
  • the layers 4 each have a layer top 6, a layer bottom 7 and one
  • Obstacle structure 8 A fluid comprising the particles 2, 3 (not shown here) can flow through the obstacle structure 8.
  • the obstacle structure 8 is arranged on the top side 6 of the layer. Also spaced the
  • Obstacle structure 8 the top layer 6 of the bottom layer 7 of the same layer 4 or a further one of the layers 4.
  • the arrangement according to FIG. 2 can be formed, for example, by stacking several layers 4 according to FIG. 1 or alternatively by winding a layer 4 according to FIG. 1.
  • the arrangement can resemble a coplanar line.
  • the effective cross-sectional area of an imaginary microfluidic channel can be defined via the distances and heights of the individual posts.
  • the obstacle structure 8 or the layer with the metal posts is rolled up and is insulated on both sides by two layers 4 of a planar one
  • Obstacle structures 8 or (obstacle structure) layers are completely shielded - winding or stacking is thus made possible in a particularly advantageous manner. This manifests itself in particular in a symmetrical and uniform electrical field 16, which can be found in each of the “cages” (partial microfluidic channels 17).
  • the shielding could also be omitted for simplified manufacture and operation. For example, only one insulator layer 13 could remain, on which the metal posts
  • inflowing particles could pass through the stacked obstacle structures 8 and thereby advantageously interact as strongly as possible with the inhomogeneous E field 16.
  • the summation of many imaginary small partial microfluidic channels 17 over the width of an entire band results in a stacked state in parallel connection to a channel with an acceptable effective cross-sectional area.
  • FIG. 2 it can also be seen that a fluid channel 9, in which the obstacle structure 8 is arranged, is formed between the top side 6 of the bottom 7 facing the layer.
  • the sum of these fluid channels 9 or the partial microfluidic channels 17 results in a flowable (total) cross-sectional area of the device, which previously also was effective
  • FIG. 3 schematically shows a layer 4 according to FIG. 1 or from FIG. 2 in a perspective view.
  • the reference numerals are used uniformly, so that reference can be made in full to the above statements relating to the preceding FIGS. 1 and 2.
  • FIG. 4 schematically shows a further layer 4 for a device proposed here in a perspective view.
  • the reference numerals are used uniformly, so that reference can be made in full to the above statements relating to the previous figures.
  • Electrode structure but an insulator structure. Instead of the metal posts, insulating spacers (posts) are used as the obstacle structure 8 with planar metal electrodes 12 applied (on one or both sides). It is particularly advantageous here if (because of possible
  • FIG. 5 schematically shows a detailed view of the exemplary embodiment according to FIG.
  • FIG. 6 schematically shows a sequence of a method proposed here.
  • the method is used to manufacture a device proposed here.
  • the sequence of the method steps shown with the blocks 110 and 120 results in a regular operating sequence.
  • A occurs in block 110
  • at least one of the layers is wrapped or several of the layers are stacked.
  • FIG. 7 illustrates the provision of a layer 4.
  • the layer 4 is held on a carrier roller 19 by way of example with a fastening means 18.
  • the fastening means 18 is simultaneously formed, for example, in the manner of a spacer.
  • FIG. 8 illustrates a winding of the layer 4 provided according to FIG. 7.
  • a layer 4 (suitably structured film arrangement) is wound onto a carrier roll 19 and electrically contacted at the end.
  • the effective cross-sectional area of such a "DEP winding cylinder" can be determined from the top surfaces of the
  • rolled-up tape and the carrier contained therein are calculated and varies depending on the layout.
  • the diameter of the carrier 19 can be minimized in favor of a maximum throughput.
  • Such a cylinder would be integrable into a likewise cylindrical channel (see FIG. 9) without major difficulties.
  • FIG. 9 schematically shows a further device 1 proposed here in a perspective view.
  • the reference symbols are used uniformly, so that reference can be made in full to the above explanations regarding the previous figures.
  • the device 1 could have been manufactured, for example, using the method steps illustrated in FIGS. 7 and 8. In other words, this means in particular that the device 1 is formed in the manner of a “winding cylinder” according to the division of darts according to FIG. 9.
  • a layer 4 (DEP tape), for example according to FIG. 1, with a thickness of 100 pm (50 pm substrate thickness and 50 pm post height with a ratio of post width to post spacing of 1: 1) and about 1 m length could have over 35 turns on a roll 19 with a diameter of 6 mm are rolled into a cylinder with a total diameter of less than 13 mm.
  • the length of such a winding cylinder could be chosen individually (e.g. 1 cm).
  • a 10 ml blood sample could then also be processed at a maximum flow rate of 100 pm / s within about an hour, with the big difference (compared to the calculation example given above for conventional DEP trapping filtering) that such a filter would then could be installed relatively easily in lab-on-chip systems.
  • the insulator layers 13 could be made with insulator foils.
  • the insulator films could be thin, spin-on polyimide films with a thickness of, for example, up to 25 ⁇ m.
  • a carrier roll 19 from z. B. Plastic could have a bend radius of up to one millimeter or less. Sheets and rolls could be connected to each other with an adhesive tape of a suitable height, which could also serve as a spacer and protection of the posts (obstacle structure 8) during a first winding.
  • a metal, such as copper, could be used as the electrode material or gold, which (previously) for example by photolithography,
  • Obstacle structure 8 was structured and applied.
  • Conductor tracks 14 could have thicknesses between a few nanometers to a few micrometers and posts of an exemplary obstacle structure 8) heights of possibly up to 100 pm.
  • Solder contacts would be conceivable for the electrical contact 5 of the layer 4. If one wanted to passivate the metal electrodes electrically with chemically inert material (as exemplary passivation 10), aluminum oxide could be evaporated for this purpose.
  • a similar effect could also be achieved by stacking several layers 4 to form a “DEP stack” with a defined cross-section.
  • FIG. 10 schematically shows a further device 1 proposed here in a perspective view.
  • the reference symbols are used uniformly, so that reference can be made in full to the above explanations regarding the previous figures.
  • FIG. 10 exemplifies the previously mentioned embodiment as a “DEP stack”. In other words, this relates in particular to a device 1 in which a plurality of the layers 4 are stacked.
  • Layers or “DEP tapes”, as previously presented, could in principle be produced in any width and length and, consequently, individually to form cylinders and stacks with any diameter, length, width and height.
  • the relative flow velocity in the fluidic channel can be reduced in a compact form, with particles keeping a maximum distance from the electrodes • Versatile layout options, as a very large selection of design parameters is available (especially with regard to the length and width of the D EP tapes used, which would be easily adjustable)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Dispersion Chemistry (AREA)
  • Electrochemistry (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Fluid Mechanics (AREA)
  • Engineering & Computer Science (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Electrostatic Separation (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)

Abstract

L'invention concerne un dispositif (1) de capture de particules (2, 3) par diélectrophorèse, comprenant une ou plusieurs couches (4) et un moyen de mise en contact (5) électrique, les couches (4) comportant respectivement une face supérieure de couche (6), une face inférieure de couche (7) et une structure d'obstacle (8), cette structure d'obstacle (8) pouvant être traversée par un fluide comportant les particules (2, 3), et ladite structure d'obstacle (8) étant ménagée sur la face supérieure de couche (6), cette structure d'obstacle (8) espaçant en outre la face supérieure de couche (6) de la face inférieure de couche (7) de la même couche (4) ou d'une autre couche (4).
EP19735269.3A 2018-07-04 2019-06-27 Dispositif de capture de particules par diélectrophorèse Pending EP3817859A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102018211001.8A DE102018211001A1 (de) 2018-07-04 2018-07-04 Vorrichtung zum dielektrophoretischen Einfang von Teilchen
PCT/EP2019/067150 WO2020007703A1 (fr) 2018-07-04 2019-06-27 Dispositif de capture de particules par diélectrophorèse

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EP3817859A1 true EP3817859A1 (fr) 2021-05-12

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EP (1) EP3817859A1 (fr)
CN (1) CN112334234A (fr)
DE (1) DE102018211001A1 (fr)
WO (1) WO2020007703A1 (fr)

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CN112334234A (zh) 2021-02-05
DE102018211001A1 (de) 2020-01-09
WO2020007703A1 (fr) 2020-01-09
US20210260602A1 (en) 2021-08-26
US11975340B2 (en) 2024-05-07

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