EP3463383A4 - Direkte umprogrammierung einer menschlichen somatischen zelle auf eine ausgewählte (vorbestimmte) differenzierte zelle mit funktionalisierten nanopartikeln - Google Patents

Direkte umprogrammierung einer menschlichen somatischen zelle auf eine ausgewählte (vorbestimmte) differenzierte zelle mit funktionalisierten nanopartikeln Download PDF

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Publication number
EP3463383A4
EP3463383A4 EP17807622.0A EP17807622A EP3463383A4 EP 3463383 A4 EP3463383 A4 EP 3463383A4 EP 17807622 A EP17807622 A EP 17807622A EP 3463383 A4 EP3463383 A4 EP 3463383A4
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EP
European Patent Office
Prior art keywords
cell
predetermined
functionalized nanoparticles
human somatic
direct reprogramming
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP17807622.0A
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English (en)
French (fr)
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EP3463383A1 (de
Inventor
Andranik Andrew Aprikyan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Stemgenics Inc
Original Assignee
Stemgenics Inc
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Filing date
Publication date
Application filed by Stemgenics Inc filed Critical Stemgenics Inc
Publication of EP3463383A1 publication Critical patent/EP3463383A1/de
Publication of EP3463383A4 publication Critical patent/EP3463383A4/de
Pending legal-status Critical Current

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    • A61K47/69—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
    • A61K47/6921—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere
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    • A61K47/6923—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere the form being an inorganic particle, e.g. ceramic particles, silica particles, ferrite or synsorb
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EP17807622.0A 2016-06-03 2017-06-02 Direkte umprogrammierung einer menschlichen somatischen zelle auf eine ausgewählte (vorbestimmte) differenzierte zelle mit funktionalisierten nanopartikeln Pending EP3463383A4 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662345360P 2016-06-03 2016-06-03
PCT/US2017/035823 WO2017210638A1 (en) 2016-06-03 2017-06-02 Direct reprogramming of a human somatic cell to a selected (predetermined) differentiated cell with functionalized nanoparticles

Publications (2)

Publication Number Publication Date
EP3463383A1 EP3463383A1 (de) 2019-04-10
EP3463383A4 true EP3463383A4 (de) 2020-02-12

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EP17807622.0A Pending EP3463383A4 (de) 2016-06-03 2017-06-02 Direkte umprogrammierung einer menschlichen somatischen zelle auf eine ausgewählte (vorbestimmte) differenzierte zelle mit funktionalisierten nanopartikeln

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US (1) US20190224335A1 (de)
EP (1) EP3463383A4 (de)
JP (2) JP2019517531A (de)
KR (1) KR20190028665A (de)
CN (2) CN114634908A (de)
AU (2) AU2017274665A1 (de)
BR (1) BR112018075051A2 (de)
CA (1) CA3026365A1 (de)
IL (1) IL263410A (de)
MX (1) MX2018014967A (de)
RU (1) RU2018146815A (de)
SG (1) SG11201810827VA (de)
WO (1) WO2017210638A1 (de)

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JP2023519078A (ja) * 2020-01-10 2023-05-10 アンドラニック アンドリュー アプリキャン， 目的の遺伝子の発現および／またはシグナル伝達経路の調節のためのナノ粒子

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013059831A1 (en) * 2011-10-21 2013-04-25 Stemgenics, Inc Functionalized nanoparticles for intracellular delivery of biologically active molecules
US20140087416A1 (en) * 2012-09-07 2014-03-27 Genentech, Inc. Methods and compositions for producing induced hepatocytes

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3112467B1 (de) * 2009-12-07 2018-02-14 The Trustees of the University of Pennsylvania Rna-präparate mit gereinigter modifizierter rna zur zellneuprogrammierung
CN103857797A (zh) * 2011-07-19 2014-06-11 帷幄生物技术公司 用于修复软骨损伤的非遗传修饰性重编程细胞的组合物和方法
US20150299698A1 (en) * 2012-11-01 2015-10-22 The Regents Of The University Of California Methods for engineering non-neuronal cells into neurons and using newly engineered neurons to treat neurodegenerative diseases

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013059831A1 (en) * 2011-10-21 2013-04-25 Stemgenics, Inc Functionalized nanoparticles for intracellular delivery of biologically active molecules
US20140087416A1 (en) * 2012-09-07 2014-03-27 Genentech, Inc. Methods and compositions for producing induced hepatocytes

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
MIN WEI ET AL: "Nanomaterials modulate stem cell differentiation: biological interaction and underlying mechanisms", JOURNAL OF NANOBIOTECHNOLOGY, vol. 15, no. 1, 25 October 2017 (2017-10-25), XP055650869, DOI: 10.1186/s12951-017-0310-5 *
NAOTO MURAOKA ET AL: "Direct Reprogramming of Fibroblasts into Myocytes to Reverse Fibrosis", ANNUAL REVIEW OF PHYSIOLOGY, vol. 76, no. 1, 10 February 2014 (2014-02-10), pages 21 - 37, XP055138589, ISSN: 0066-4278, DOI: 10.1146/annurev-physiol-021113-170301 *
VAIBHAV PANDIT ET AL: "Multilayered Nanoparticles for Gene Delivery Used to Reprogram Human Foreskin Fibroblasts to Neurospheres", TISSUE ENGINEERING. PART C, METHODS DEC 2008, vol. 21, no. 8, 1 August 2015 (2015-08-01), US, pages 786 - 794, XP055358654, ISSN: 1937-3384, DOI: 10.1089/ten.tec.2014.0482 *
XIAO CHEN ET AL: "Nanoparticle delivery of stable miR-199a-5p agomir improves the osteogenesis of human mesenchymal stem cells via the HIF1a pathway", BIOMATERIALS., vol. 53, 1 June 2015 (2015-06-01), GB, pages 239 - 250, XP055651275, ISSN: 0142-9612, DOI: 10.1016/j.biomaterials.2015.02.071 *
Y.-J. NAM ET AL: "Reprogramming of human fibroblasts toward a cardiac fate", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 110, no. 14, 4 March 2013 (2013-03-04), pages 5588 - 5593, XP055210948, ISSN: 0027-8424, DOI: 10.1073/pnas.1301019110 *
YIMIN ZHAO ET AL: "Microarc-oxidized titanium surfaces functionalized with microRNA-21-loaded chitosan/hyaluronic acid nanoparticles promote the osteogenic differentiation of human bone marrow mesenchymal stem cells", INTERNATIONAL JOURNAL OF NANOMEDICINE, vol. 2015, no. 10, 27 October 2015 (2015-10-27), pages 6675 - 6687, XP055589035, DOI: 10.2147/IJN.S94689 *

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CN114634908A (zh) 2022-06-17
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AU2017274665A1 (en) 2018-12-20
RU2018146815A3 (de) 2020-10-15
IL263410A (en) 2018-12-31
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