EP3380599B1 - Composition détergente liquide pour le lavage - Google Patents

Composition détergente liquide pour le lavage Download PDF

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Publication number
EP3380599B1
EP3380599B1 EP16794255.6A EP16794255A EP3380599B1 EP 3380599 B1 EP3380599 B1 EP 3380599B1 EP 16794255 A EP16794255 A EP 16794255A EP 3380599 B1 EP3380599 B1 EP 3380599B1
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EP
European Patent Office
Prior art keywords
detergent composition
protease
liquid
liquid detergent
amino acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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EP16794255.6A
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German (de)
English (en)
Other versions
EP3380599A1 (fr
Inventor
Gleb Urevich PRIIMOV
Matthew Peter WRIGHT
Alastair Richard Sanderson
Vincent Charles Mole
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Unilever PLC
Unilever NV
Original Assignee
Unilever PLC
Unilever NV
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Priority claimed from PCT/EP2016/076639 external-priority patent/WO2017089093A1/fr
Publication of EP3380599A1 publication Critical patent/EP3380599A1/fr
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38618—Protease or amylase in liquid compositions only
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21062—Subtilisin (3.4.21.62)

Definitions

  • a gene encoding a subtilase variant may be obtained by mutation or DNA shuffling techniques known in the art from the gene encoding the wild-type subtilase.
  • a substitution means replacement of the amino acid occupying a position with a different amino acid;
  • a deletion means removal of the amino acid occupying a position;
  • an insertion means adding one or more (e.g., several) amino acids, e.g., 1, 2, 3, 4 or 5 amino acids adjacent to and immediately following the amino acid occupying a position.
  • Variants comprising multiple alterations are separated by addition marks ("+"), e.g., "Arg170Tyr+Gly195Glu” or "R170Y+G195E” representing a substitution of arginine and glycine at positions 170 and 195 with tyrosine and glutamic acid, respectively.
  • multiple alterations may be separated be space or a comma, e.g., A170Y G195E or A170Y, G195E respectively.
  • the term "contiguous” or “consecutive”, with regard to nucleic acid or amino acid sequences described herein, means to be connected in an unbroken sequence.
  • first sequence to comprise 30 contiguous (or consecutive) amino acids of a second sequence means that the first sequence includes an unbroken sequence of 30 amino acid residues that is 100% identical to an unbroken sequence of 30 amino acid residues in the second sequence.
  • first sequence to have "100% identity" or being "100% identical" with a second sequence means that the first sequence exactly matches the second sequence with no gaps between nucleotides or amino acids.
  • conservative substitutions are within the groups of basic amino acids (arginine, lysine and histidine), acidic amino acids (glutamic acid and aspartic acid), polar amino acids (glutamine and asparagine), hydrophobic amino acids (leucine, isoleucine and valine), aromatic amino acids (phenylalanine, tryptophan and tyrosine), and small amino acids (glycine, alanine, serine, threonine and methionine).
  • Amino acid substitutions that do not generally alter specific activity are known in the art and are described, for example, by H. Neurath and R.L. Hill, 1979, In, The Proteins, Academic Press, New York .
  • Preferred cationic surfactants are quaternary ammonium salts of the general formula R 1 R 2 R 3 R 4 N + X - , for example where R 1 is a C 12 -C 14 alkyl group, R 2 and R 3 are methyl groups, R 4 is a 2-hydroxyethyl group, and X - is a chloride ion.
  • This material is available commercially as Praepagen (Trade Mark) HY from Clariant GmbH, in the form of a 40 % w/w aqueous solution.
  • Sulfobetaines such as: 3-(dodecyldimethyl-ammonium)-2-hydroxy-1 -propane sulfonate; 3 -(tetradecyldimethylammonium)-1-propane sulfonate; 3-(C 12 -C 14 alkyl-amidopropyldimethylammonium)-2-hydroxy-1-propane sulfonate; and 3-(cocodimethylammonium)-1-propane sulfonate.
  • a highly preferred surfactant system comprises two different anionic surfactants, preferably linear alkyl benzene sulphonate and a sulphate, for example LAS and SLES.
  • the liquid detergent composition of the invention comprises calcium, preferably at a concentration of 0.01% - 0.3% w/w, 0.05% - 2% w/w, or 0.05 - 1.5% w/w, such as about 0.05 or about 0.15% w/w, CaCl 2 .2H 2 0 (or an equivalent % of an alternative calcium (II) source).
  • Example 1 Preparation and expression of a subtilase variant

Claims (11)

  1. Composition de détergent liquide comprenant une protéase ayant une séquence d'acide aminé qui présente 100 % d'identité de séquence par rapport à SEQ ID NO : 1, dans laquelle la protéase est un variant de subtilase comprenant les substitutions X9E+X206L+X262E.
  2. Composition de détergent liquide selon la revendication 1, dans laquelle la protéase comprend de plus la substitution X76D.
  3. Composition de détergent liquide selon la revendication 1 ou 2, dans laquelle la protéase comprend de plus une ou plusieurs altérations choisies dans le groupe consistant en X3T, X4I, X14T, X24G, X24R, X27R, *36D, X43A, X43C, X43L, X43R, X43W, X68A, X72A, X72V, X78D, X87R, X87S, *97E, X98S, X99A, X99D, X99A, X99D, X99E, X99G, *99D, X101D, X101E, X101G, X101I, X101K, X101L, X101M, X101N, X101R, X103A, X104F, X104I, X104N, X104Y, X106A, X114V, X115T, X115W, X118R, X118V, X120D, X120I, X120N, X120T, X120V, X123S, X128A, X128L, X128S, X129D, X129N, X129Q, X130A, X147W, X149C, X149N, X158E, X160D, X160P, X161C, X161E, X162L, X163A, X163D, X167A, X170S, X182C, X182E, X185C, X185E, X188C, X188D, X188E, X191N, X194P, X195E, X199M, X204D, X204V, X205I, X209W, X212A, X212D, X212G, X212N, X216I, X216T, X216V, X217C, X217D, X217E, X217M, X217Q, X217Y, X218D, X218E, X218T, X222C, X222R, X222S, X225A, X232V, X235L, X236H, X245K, X245R, X252K, X255C, X255E, X256A, X256C, X256D, X256V, X256Y, X259D, X260E, X260P, X261C, X261E, X261F, X261L, X261M, X261V, X261W, X261Y et X274A.
  4. Composition de détergent liquide selon l'une quelconque des revendications 1-3, dans laquelle la protéase comprend de plus 1, 2, 3, 4, 5, 6, ou la totalité des substitutions choisies dans le groupe consistant en : X43R, X185E, X188E, X191N, X194P, X206L, X209W, X259D.
  5. Composition de détergent liquide selon l'une quelconque des revendications 1-4, dans laquelle la protéase est un variant d'une subtilase provenant de Bacillus lentus.
  6. Composition de détergent liquide selon l'une quelconque des revendications 1-5, qui comprend une ou plusieurs enzymes supplémentaires choisies dans le groupe consistant en amylases, catalases, cellulases (par exemple, endoglucanases), cutinases, haloperoxygénases, lipases, mannanases, pectinases, pectine lyases, peroxydases, protéases, xanthanases, et xyloglucanases, ou un mélange quelconque de celles-ci.
  7. Composition de détergent liquide selon la revendication 1, qui comprend un inhibiteur de protéase à base de peptide, dans laquelle l'inhibiteur de protéase à base de peptide présente la structure suivante : des composés de peptides de la formule B2-B1-B0-R, dans laquelle
    • R est l'hydrogène, CH3, CX3, CHX2 ou CH2X, où X est un atome d'halogène ;
    • B0 est un résidu de phénylalanine avec un substituant OH à la position p et/ou à la position m;
    • B1 est un résidu d'acide aminé simple ; et
    B2 consiste en un ou plusieurs résidus d'acides aminés, comprenant éventuellement un groupe de protection N-terminal.
  8. Composition de détergent liquide selon l'une quelconque des revendications 1 à 7, dans laquelle la composition de détergent est emballée dans la forme d'une composition de détergent liquide emballée en dose unitaire.
  9. Composition de détergent liquide selon l'une quelconque des revendications 1 à 8, dans laquelle la quantité totale de tensioactif est de 0,1 à 85 % en masse/masse, de préférence de 1 à 60 % en masse/masse, encore mieux de 2 à 40 % en masse/masse, bien mieux encore de 3 à 35 % en masse/masse et particulièrement de préférence de 5 à 20 % en masse/masse.
  10. Composition de détergent liquide selon l'une quelconque des revendications 1 à 9, dans laquelle la composition de détergent liquide est une composition de lavage de vaisselle liquide ou une composition de lessive liquide, de préférence est une composition de lave-vaisselle liquide ou une composition de lessive liquide, et est encore mieux une composition de lessive liquide.
  11. Procédé de lessive utilisant une composition de détergent de lessive liquide comprenant une protéase présentant une séquence d'acide aminé qui présente 100 % d'identité de séquence par rapport à SEQ ID NO : 1, dans lequel la protéase est un variant de subtilase comprenant les substitutions X9E+X206L+X262E, comprenant l'étape de mise en contact du textile avec la composition de détergent de lessive liquide dans des conditions appropriées pour nettoyer le textile.
EP16794255.6A 2015-11-25 2016-11-04 Composition détergente liquide pour le lavage Active EP3380599B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP2015196270 2015-11-25
PCT/EP2016/076639 WO2017089093A1 (fr) 2015-11-25 2016-11-04 Composition de détergent liquide

Publications (2)

Publication Number Publication Date
EP3380599A1 EP3380599A1 (fr) 2018-10-03
EP3380599B1 true EP3380599B1 (fr) 2019-03-06

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Family Applications (1)

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Country Status (2)

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EP (1) EP3380599B1 (fr)
TR (1) TR201904150T4 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3976776A1 (fr) 2019-05-24 2022-04-06 Danisco US Inc. Variants de subtilisine et procédés d'utilisation
WO2023114936A2 (fr) 2021-12-16 2023-06-22 Danisco Us Inc. Variants de subtilisine et procédés d'utilisation
WO2023114932A2 (fr) 2021-12-16 2023-06-22 Danisco Us Inc. Variants de subtilisine et procédés d'utilisation
WO2023114939A2 (fr) 2021-12-16 2023-06-22 Danisco Us Inc. Variants de subtilisine et procédés d'utilisation
WO2024050343A1 (fr) 2022-09-02 2024-03-07 Danisco Us Inc. Variants de subtilisine et procédés associés
WO2024050346A1 (fr) 2022-09-02 2024-03-07 Danisco Us Inc. Compositions détergentes et procédés associés

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
None *

Also Published As

Publication number Publication date
TR201904150T4 (tr) 2019-05-21
EP3380599A1 (fr) 2018-10-03

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