EP3183060B1 - Préstockage de réactifs avec prélèvement défini - Google Patents

Préstockage de réactifs avec prélèvement défini Download PDF

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Publication number
EP3183060B1
EP3183060B1 EP15756364.4A EP15756364A EP3183060B1 EP 3183060 B1 EP3183060 B1 EP 3183060B1 EP 15756364 A EP15756364 A EP 15756364A EP 3183060 B1 EP3183060 B1 EP 3183060B1
Authority
EP
European Patent Office
Prior art keywords
opening
membrane
closure means
receiving region
chamber
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP15756364.4A
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German (de)
English (en)
Other versions
EP3183060A1 (fr
Inventor
Sven Zinober
Yvonne Beyl
Daniel Czurratis
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Robert Bosch GmbH
Original Assignee
Robert Bosch GmbH
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Filing date
Publication date
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Publication of EP3183060A1 publication Critical patent/EP3183060A1/fr
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Publication of EP3183060B1 publication Critical patent/EP3183060B1/fr
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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/52—Containers specially adapted for storing or dispensing a reagent
    • B01L3/523—Containers specially adapted for storing or dispensing a reagent with means for closing or opening
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/044—Connecting closures to device or container pierceable, e.g. films, membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/046—Function or devices integrated in the closure
    • B01L2300/049—Valves integrated in closure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0638—Valves, specific forms thereof with moving parts membrane valves, flap valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0655—Valves, specific forms thereof with moving parts pinch valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0677—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0677—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
    • B01L2400/0683—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers mechanically breaking a wall or membrane within a channel or chamber

Definitions

  • reagents In microfluidics, reagents must be stored for a longer period of time for later use.
  • the pre-storage is usually carried out in specially prepared chambers, with inflows and outflows of the chambers must be sealed fluid-tight.
  • US 7,980,272 B2 For example, a microfluidic device with a sample chamber is known, wherein the opening is closed in the chamber with a removable material by heating.
  • US Pat. No. 6,082,185 A describe a microfluidic device for analyzing samples with closure means for temporarily closing openings.
  • the invention relates to a device, in particular a microfluidic device, having a chamber for receiving a fluid, wherein the chamber has a wall with an opening and wherein the opening is closed by a sealing means impermeable to predetermined substances.
  • the predetermined substances are preferably in fluid form or are dissolved in a fluid.
  • the device comprises a membrane, wherein the membrane in a region of an outer side of the wall, which adjoins the opening, bears against the outside of the wall and covers the opening.
  • the invention thus provides an advantageous device, which by means of the impermeable for predetermined substances closure means a Long-term stable storage of these substances allows.
  • an uncontrolled opening of the chamber is prevented at a removal of the closure means from the opening.
  • the invention prevents an uncontrolled escape of the predetermined substances, the upstream fluid and / or the closure means from the chamber and allows a defined removal of the fluid via a, preferably controlled, deflection of the membrane over the opening.
  • a removal of the closure means can be done in particular by a previously made at least partial heating of the closure means.
  • a connection between a first part of the closure means and a part of a boundary of the opening and / or between a first part of the closure means and a second part of the closure means is achieved.
  • the dissolved part can be removed from the opening.
  • the device has a fluid channel which extends at least partially between the outside of the wall and the membrane such that, given a predetermined deflection of a first section of the membrane over the opening, in particular into a first receiving region, the fluid channel is fluidically connected to the opening.
  • the device comprises a side facing away from the opening of the membrane adjacent the second receiving area.
  • the second receiving region has a minimum size in order, in the case of a predetermined expansion of the first section or of a second section of the membrane over the opening into the first receiving region, to release the dissolved part of the closure device into the first receiving region together with the first section or with the second section Record membrane.
  • the first receiving area and / or the second receiving area have a pneumatic access for setting a negative pressure in the first receiving area or in the second receiving area.
  • the pneumatic access thus makes it possible to deflect the first section or the second section of the membrane into the first receiving region or into the second receiving region by the set negative pressure.
  • the pneumatic access is an opening or a valve in a wall bounding the first or second receiving area.
  • the pneumatic access is coupled to a pump for generating the negative pressure in the first receiving area or in the second receiving area, wherein the pump may be part of the device according to the invention and / or connected for example via a fluid-tight line to the respective pneumatic access.
  • the device preferably comprises a layer adjoining the opening of the membrane, the layer having the first receiving area and / or the second receiving area in the form of a recess.
  • the second receiving area has a constriction so that a partial area of the second receiving area located behind the constriction relative to the membrane has the minimum size for receiving the first section or the second section of the membrane and the dissolved part of the closure means.
  • This restriction makes it difficult for the accommodated part of the closure means to move back in the direction of the opening of the wall, thereby hindering removal of the predetermined substances from the chamber.
  • the opening has a predetermined shape for a targeted removal of the dissolved part of the closure means from the opening.
  • the predetermined shape advantageously supports a movement of the dissolved part in a preferred direction, for example in the direction of the first receiving area.
  • the opening in the wall of the chamber may have a width which increases or decreases in the direction of the outside of the wall.
  • the broadening or reduction can be carried out in the form of a cone, a pyramidal or a tetrahedral stump.
  • the chamber is filled with a fluid, wherein the fluid has a greater or lesser density than the closure means, so that in the at least partial removal of the closure means from the opening of the part of the closure means in a direction the gravity-related upper portion of the device rises or falls in a direction of gravity related lower portion of the device.
  • the closure means comprises paraffin.
  • Paraffin is particularly suitable for sealing in microfluidics because it is inert to many chemicals and insoluble and harmless to health. Another advantage is that paraffin has a low melting point and thus supports the removal of a part of the closure means via heating. By choosing the composition of the paraffin from different alkanes, the melting point of the paraffin can be adjusted.
  • the closure means comprises metals or metal compounds for inductive heating. Since microfluidic devices are largely constructed of electrically non-conductive materials, heating of the closure means via electromagnetic induction is particularly advantageous. This means that closure means which are located inside a microfluidic device and thus are difficult to access from the outside to heat radiation can be heated in a simple manner, without an environment of the closure means being excessively heated.
  • the closure means comprises predefined substances, the substances having an absorptivity greater than 0.8, preferably greater than 0.9, for electromagnetic radiation having a wavelength between 780 and 3000 nm, preferably between 900 and 1100 nm.
  • the use of soot particles in the closure agent is particularly advantageous here.
  • the chamber has at least partially an inner coating with a material impermeable to given substances.
  • the inner coating preferably comprises a paraffin, metal layers with aluminum, aluminum oxide, platinum, silver, gold, silicon dioxide layers and / or plasma-polymerized hexamethyldisiloxane layers.
  • the invention also relates to a method for removing a fluid from a device according to the invention.
  • the method comprises in a first step a solution, in particular by heating the closure means, at least part of the closure means from the opening of the chamber.
  • the first section of the membrane is deflected over the opening, in particular into the first receiving area, in order to fluidically connect the fluid channel to the opening.
  • at least a portion of the fluid received in the chamber is removed.
  • the first section or the second section of the membrane is deflected into the second receiving area before the expansion of the first section of the membrane, to the dissolved part of the closure means together with the first section or to record with the second portion of the membrane in the second receiving area.
  • the invention also relates to a manufacturing method for a device according to the invention.
  • the manufacturing method comprises in a first step connecting a first substrate having the chamber for receiving the fluid with the opening, with the membrane, so that the membrane rests against a first side of the substrate and covers the opening.
  • the membrane is connected to the layer which has the first and / or the second receiving region for receiving the deflecting first section of the membrane as a recess, via the side of the membrane which faces away from the opening.
  • the opening is closed by means of a dispenser introduced into the chamber via an at least partially open side of the substrate with the closure means.
  • the at least partially open side of the substrate is sealed with a sealing film.
  • FIG. 1 a part of a device 10 according to the invention is shown, which has a chamber 20 for receiving a fluid 21.
  • the chamber 20 is bounded on one side by a wall 30, wherein the chamber 20 has an opening 40 which is closed with a sealing means 50 impermeable to predetermined substances.
  • the device 10 comprises a membrane 60, wherein the membrane 60 in a region of an outer side of the wall 30, which adjoins the opening 40, rests against the outside of the wall 30 and covers the opening 40. Due to the covering of the opening 40 by the membrane 60, removal of the closure means 50 prevents at least immediate leakage of the fluid 21 from the chamber 20, even if the membrane 60 is not completely vapor impermeable to the substances present in the fluid 21.
  • a coating 14 may be applied to inner sides of walls of the chamber 20, which prevents diffusion of substances from the fluid 21 through the walls of the chambers and thus supports a long-term stable storage of the fluid 21.
  • thermoplastics such as polycarbonates (PC), polypropylene (PP), polyethylene (PE), polymethyl methacrylate (PMMA) or cyclo-olefin copolymers (COC, COP), with a preferred thickness of 0 , 5 to 5 mm.
  • the membrane 60 may be embodied as a 0.005 to 0.3 mm thick polymer membrane and may include, for example, an elastomer, a thermoplastic elastomer, a thermoplastic, or a heat sealable film.
  • the volume of the chamber 20 is, for example, between 0.005 and 50 ml.
  • the optional coating 14 may include paraffin, metal layers with aluminum, alumina, platinum, silver, gold, silicon dioxide layers, and / or plasma polymerized hexamethyldisiloxane layers.
  • the closure means 50 may comprise softened or hard paraffins with melting or solidification points which can be predetermined by a choice of the alkane composition, whereby it is possible in a simple manner to close the closure means 50 by means of a heat source 100 arranged near the opening 40 by heating the closure means 50 To remove opening 40.
  • a heating of the paraffin-containing closure means 50 via the solidification point of the paraffin used causes a softening and thus a detachment of the closure means 50 from the opening 40 by the onset of fusion.
  • the heat source 100 for heating a part or the entire closure means 50 may comprise an external heating element, for example a laser, or else a heating element embedded in the device 10 according to the invention, for example a heating resistor integrated near the closure means 50.
  • the closure means 50 may also comprise metals or metal compounds, so that heating of the closure means 50 via electromagnetic induction can take place.
  • the closure means 50 may include a metal structure.
  • the closure means 50 may also include substances having a high degree of electromagnetic radiation absorption in a predetermined wavelength range to assist rapid and efficient heating of the closure means 50.
  • paraffin-embedded soot particles are suitable here.
  • FIG. 1b shows the inventive device FIG. 1a wherein the closure means 50 has been removed from the opening 40.
  • the closure means 50 has a lower density than the fluid 21 stored in the chamber 20, so that the closure means 50 has risen in an upper region of the chamber 20 with respect to the direction 200 of gravity and thus the opening 40 is exposed. Since the membrane 60 covers the opening 40, leakage of the fluid 21 from the chamber 20 is prevented.
  • the fluid 21 can be removed from the chamber 20 due to the known size of the opening 40 in well-defined quantities subsequently.
  • the device 10 according to the invention further comprises a fluid channel 70 which extends at least partially between the outside of the wall 30 and the membrane 60 such that at a predetermined deflection of a first portion 61 of the membrane 60 above the opening 40, the fluid channel 70 with the Opening 40 is fluidly connected.
  • the deflection of the first portion 61 of the membrane 60 can be effected by applying a negative pressure.
  • the device 10 according to the invention comprises a layer 80 adjacent to the opening 40 of the membrane 60, with a first receiving region 91 for receiving the deflecting first section 61 of the membrane 60.
  • the membrane 60 may be joined to the wall 60 and / or the membrane 80 adjacent layer at those locations where deflection of the membrane 60 is not desired.
  • a pneumatic access 90 in the form of a first passage opening 90 in the layer 80 to the first receiving area 91 a negative pressure for the deflection of the first portion 61 of the membrane 60 can be applied.
  • the passage opening 90 as in FIG. 1b shown connected to a pump 110, for example.
  • the fluid 21 located in the chamber 20 can be removed via the fluid channel 70.
  • a predetermined shape of the opening 40 is characterized by a decreasing toward the outside of the wall 30 width of the opening 40. Furthermore, the opening 40 is bounded by a first surface 41, wherein the first surface 41 is inclined at a predetermined for the operation of the device orientation of the device with respect to the direction 200 of the acting buoyancy and / or gravity.
  • the included by the surface normal of the first surface 41 and the direction of the acting buoyancy and / or gravity angle is greater than 0 ° and less than 180 ° and is preferably between 30 ° and 60 °, more preferably about 45 °.
  • the opening 40 may in particular be shaped such that the closure means 50 located therein, which completely fills the opening, has the shape of a cone, a pyramid or a tetrahedral stocking.
  • Such an inclined surface 41 of the opening 40 in cooperation with the buoyancy and / or gravity, predetermines a preferred direction 51 for the guidance of the released closure means 50.
  • the inclined first surface 41 effects a buoyancy force for the outflowing closure means 50 that is directed towards the interior of the chamber 20. If the closure means 50 is only softened by heating but not changed in shape, this prevents 40 decreasing width of the softened closure means 50 from the chamber. Furthermore, the buoyancy force resulting at the inclined first surface 41 causes a targeted removal of the closing means 50 from the opening 40 into the interior of the chamber 20th
  • the opening 40 has a width which increases in the direction of the outside of the wall 30. This has the advantageous effect that in a solution of the connection between the closure means 50 and the opening 40, the closure means 50 is initially hindered from moving into the chamber 20 and remains in the opening 40. After a deflection of the first section 61 of the membrane 60, the closure means 50 can be led away from the opening 40 via the fluid channel 70.
  • FIGS. 2a to 2d a manufacturing method for the device 10 according to the invention is shown as a polymeric multilayer structure.
  • the device 10 is manufactured by connecting a first substrate 15, which has the chamber 20 in the form of a recess with the opening 40, to the flexible membrane 60 so that the membrane 60 bears against a first side of the substrate 15 and the opening 40, and by connecting the membrane 60 with the layer 80 via the side facing away from the opening 40 of the membrane 60.
  • the layer 80 has a receiving area 91, 92 for receiving the deflecting first portion 61 of the membrane 60 as a recess.
  • FIG. 2b shows how the opening 40 is closed with the closure means 50 by means of a dispenser 300 introduced into the chamber 20 via the at least partially open second side 16, before, as in FIG Figure 2c shown, the chamber 20 is filled with the fluid 21 via the dispenser 300.
  • FIG. 3 shows how the open side 16 of the substrate 15 and thus the chamber 20 are sealed in a fluid-tight manner by gluing or welding with a sealing film 17, for example a polymer composite film or a polymer plate.
  • FIGS. 3a to 3c a development of the device 10 according to the invention is shown based on the in the FIGS. 1a to 1c illustrated embodiment.
  • the development differs from the illustrated embodiment of FIGS. 1a to 1c through an opening 40, wherein the opening 40 has a width which increases in the direction of the outside of the wall 30.
  • the enlarging width is indicated adjacent to the first surface 41 by a second aperture 40 delimiting surface 42, the first surface 41 and the second surface 42 being oriented with respect to the direction 200 of the acting buoyancy at a device orientation of the device. and / or gravity are inclined.
  • the included by the surface normals of the first surface 41 and the second surface 42 and the direction of the acting buoyancy and / or gravity angle is greater than 0 ° and less than 180 ° and is preferably between 30 ° and 60 °, more preferably about 45 °.
  • the opening 40 thus has, due to the inclined from the bottom of the opening 40 inclined second surface 42 and from above the opening 40 bounding inclined Surface 41 a 30 in the direction of the outside of the wall enlarging width. Due to the inclination of the first and second surfaces 41, 42, in cooperation with the buoyant force in the fluid 21, slipping or draining of the softened or sealed closure means 50 along the first and second surfaces 41, 42 toward the outside of the wall 30 is promoted.
  • the membrane 60 has a second section 62, wherein the first section 61 of the membrane 60 can be deflected into the first receiving region 91 of the layer 80 and the second section 62 can be deflected into a second receiving region 92 of the layer 80.
  • a further pneumatic access 93 in the form of a second passage opening 93 in the layer 80 to the second receiving area 92 Via a further pneumatic access 93 in the form of a second passage opening 93 in the layer 80 to the second receiving area 92, a negative pressure for the deflection of the second portion 62 of the membrane 60 can be applied.
  • the second receiving area 92 has a minimum size for receiving the removed closing means 50 together with the second portion 62 when the second portion 62 is deflected into the second receiving area 92. According to the in the FIGS.
  • the removal of the fluid 21 from the chamber 20 can take place in two steps.
  • the closure means 50 is heated via the heat source 100 and received by deflection of the second portion 62 of the membrane 60 into the second receiving region 92.
  • a fluidic connection of the chamber 20 to the fluid channel 70 is produced by deflection of the first section 61 of the membrane 60 into the first receiving region 91.
  • the second receiving region 92 of the layer 80 comprises a constriction 94, the constriction 94 being arranged in the recess 92 in such a way that a partial region 95 of the second receiving region 92 located behind the constriction 94 relative to the membrane 60 has a minimum size for receiving the second portion 62 of the membrane 60 together with the removed closure means 50.
  • FIG. 5 an embodiment of the inventive method 500 for removing a fluid 21 from the chamber 20 of the device 10 according to the invention is shown.
  • a first step 501 at least part of the closure means 50 is released from the opening 40 by heating and removed.
  • the first portion 61 of the membrane 60 is deflected over the opening 40 to fluidly connect the fluid channel 70 with the opening 40.
  • a third step 503 at least part of the fluid 21 received in the chamber 20 is removed.
  • the second portion 62 of the membrane 60 may be deflected into the second receiving area 92 to receive the remote closure means into the second receiving area 92 along with the second portion 62 of the membrane 60.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Reciprocating Pumps (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)

Claims (13)

  1. Dispositif (10), en particulier dispositif microfluidique, avec une chambre (20) destinée à contenir un fluide (21), dans lequel la chambre (20) présente une paroi (30) avec une ouverture (40) et dans lequel l'ouverture (40) est fermée avec un moyen de fermeture amovible (50) imperméable à des substances prédéterminées, caractérisé en ce que le dispositif (10) comprend une membrane (60), dans lequel la membrane (60) s'applique, dans une région d'un côté extérieur de la paroi (30) qui est adjacente à l'ouverture (40), sur le côté extérieur de la paroi (30) et recouvre l'ouverture (40), caractérisé en ce que le dispositif (10) comprend un canal de fluide (70), qui s'étend au moins en partie entre le côté extérieur de la paroi (30) et la membrane (60), de telle manière que, pour une déviation prédéterminée d'une première partie (61) de la membrane (60) au-dessus de l'ouverture (40), en particulier dans une première région de réception (91), le canal de fluide (70) soit raccordé fluidiquement à l'ouverture (40).
  2. Dispositif (10) selon l'une quelconque des revendications précédentes, dans lequel le dispositif (10) comprend une deuxième région de réception (92) adjacente au côté de la membrane (60) détourné de l'ouverture (40), dans lequel la deuxième région de réception (92) présente une grandeur minimale, pour contenir, lors d'une déviation prédéterminée de la première partie (61) de la membrane (60) ou d'une deuxième partie (62) de la membrane (60) au-dessus de l'ouverture (40) dans la deuxième région de réception (92), une partie du moyen de fermeture (50) dissoute hors de l'ouverture (40), en particulier par un chauffage du moyen de fermeture (50), de concert avec la première partie (61) ou la deuxième partie (62) de la membrane (60) .
  3. Dispositif (10) selon la revendication 2, dans lequel la première région de réception (91) et/ou la deuxième région de réception (92) présentent un accès pneumatique (90, 93) pour le réglage d'une dépression dans la première région de réception (90) ou dans la deuxième région de réception, afin de provoquer par la dépression réglée la déviation de la première partie (61) ou de la deuxième partie (62) de la membrane dans la première région de réception (91) ou dans la deuxième région de réception (92).
  4. Dispositif (10) selon la revendication 2 ou 3, dans lequel le dispositif (10) comprend une couche (80) adjacente au côté de la membrane (60) détourné de l'ouverture (40), dans lequel la couche (80) présente la première région de réception (91) et/ou la deuxième région de réception sous la forme d'une découpe.
  5. Dispositif (10) selon l'une quelconque des revendications 2 à 4, dans lequel la deuxième région de réception (92) présente un rétrécissement (94), de telle manière qu'une région partielle (95) de la deuxième région de réception (92) se trouvant après le rétrécissement (94) par rapport à la membrane (60) présente la grandeur minimale pour la réception de la première partie (61) ou de la deuxième partie (62) de la membrane (60) et de la partie dissoute du moyen de fermeture (50).
  6. Dispositif (10) selon l'une quelconque des revendications précédentes, dans lequel l'ouverture (40) présente une forme prédéterminée pour une évacuation ciblée de la partie dissoute du moyen de fermeture (50) hors de l'ouverture (40), dans lequel la forme prédéterminée est caractérisée par une largeur de l'ouverture (40) croissante ou décroissante en direction du côté extérieur de la paroi (30).
  7. Dispositif (10) selon l'une quelconque des revendications précédentes, dans lequel la chambre (20) est remplie d'un fluide (21) et le fluide (21) présente une densité plus élevée ou plus faible que le moyen de fermeture (50), de telle manière que la partie dissoute du moyen de fermeture (50) monte dans une région supérieure du dispositif (10) par rapport à la direction de la gravité ou descende dans une région inférieure du dispositif (10) par rapport à la direction de la gravité.
  8. Dispositif (10) selon l'une quelconque des revendications précédentes, dans lequel le moyen de fermeture (50) comprend de la paraffine.
  9. Dispositif (10) selon l'une quelconque des revendications précédentes, dans lequel le moyen de fermeture (50) comprend des métaux ou des composés métalliques pour un chauffage par induction.
  10. Dispositif (10) selon l'une quelconque des revendications précédentes, dans lequel la chambre (20) présente au moins en partie un revêtement intérieur (14) avec un matériau imperméable à des substances prédéterminées.
  11. Procédé (500) pour le prélèvement d'un fluide (21) hors d'une chambre (20), en particulier microfluidique, dans lequel la chambre (20) présente une paroi (30) avec une ouverture (40) et dans lequel l'ouverture (40) est fermée avec un moyen de fermeture amovible (50), imperméable à des substances prédéterminées, comprenant:
    a. la dissolution (501), en particulier par chauffage du moyen de fermeture (50), d'au moins une partie du moyen de fermeture (50) hors de l'ouverture (40),
    b. la déviation (502) d'une première partie (61) d'une membrane (60) appliquée sur un côté extérieur de la paroi (30) et recouvrant l'ouverture (40), en particulier dans une première région de réception (91), afin de relier un canal de fluide (70), qui s'étend au moins en partie entre le côté extérieur de la paroi (30) et la membrane (60), fluidiquement à l'ouverture (40),
    c. le prélèvement (503) d'au moins une partie du fluide (21) contenu dans la chambre (20) par l'ouverture (40) et le canal de fluide (70).
  12. Procédé selon la revendication 11, dans lequel on effectue avant la déviation (502) de la première partie de la membrane (60) une déviation (5011) d'une deuxième partie (62) de la membrane (60) dans une deuxième région de réception (92) adjacente au côté de la membrane (60) détourné de l'ouverture (40), afin de recevoir la partie dissoute du moyen de fermeture (50) de concert avec la deuxième partie (62) de la membrane (60) dans la deuxième région de réception (92).
  13. Procédé de fabrication d'un dispositif (10), en particulier microfluidique, avec une chambre (20) destinée à contenir un fluide (21), comprenant
    a. la liaison d'un premier substrat (15), qui présente la chambre (20) destinée à recevoir le fluide (21) avec une ouverture (40), avec une membrane (60), de telle manière que la membrane (60) s'applique sur un premier côté du substrat (15) et recouvre l'ouverture (40),
    b. la liaison de la membrane (60) avec une couche (80) par le côté de la membrane (60) détourné de l'ouverture (40), dans lequel la couche (80) présente une région de réception (91, 92) pour recevoir une partie déviée (61, 62) de la membrane (60) pour une liaison fluidique de l'ouverture (40) avec un canal de fluide (70) comme évidement,
    c. la fermeture de l'ouverture (40) au moyen d'un distributeur (300) introduit au moins en partie dans la chambre (20) par un deuxième côté au moins partiellement ouvert (16) du substrat (15) avec un moyen de fermeture (50),
    d. le scellage du deuxième côté au moins partiellement ouvert (16) du substrat (15) avec une feuille de scellage (17).
EP15756364.4A 2014-08-19 2015-08-07 Préstockage de réactifs avec prélèvement défini Active EP3183060B1 (fr)

Applications Claiming Priority (2)

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DE102014216391.9A DE102014216391A1 (de) 2014-08-19 2014-08-19 Reagenzienvorlagerung mit definierter Entnahme
PCT/EP2015/068255 WO2016026718A1 (fr) 2014-08-19 2015-08-07 Préstockage de réactifs avec prélèvement défini

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DE (1) DE102014216391A1 (fr)
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US6082185A (en) * 1997-07-25 2000-07-04 Research International, Inc. Disposable fluidic circuit cards
US20030013205A1 (en) * 2001-07-06 2003-01-16 Franz Konrad Separating device
US8852862B2 (en) * 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
US7980272B2 (en) 2007-06-21 2011-07-19 Samsung Electronics Co., Ltd. Microfluidic valve, method of manufacturing the same, and microfluidic device comprising the microfluidic valve
EP2138233B1 (fr) * 2008-06-02 2010-10-20 Boehringer Ingelheim microParts GmbH Structure de feuille microliquide destinée au dosage de liquides
JP5401542B2 (ja) * 2008-06-19 2014-01-29 ベーリンガー インゲルハイム マイクロパーツ ゲゼルシャフト ミット ベシュレンクテル ハフツング 流体計量容器
AU2011212916B2 (en) * 2010-02-03 2015-07-02 President And Fellows Of Harvard College Devices and methods for multiplexed assays
DE102010061909A1 (de) * 2010-11-24 2012-05-24 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Fluidischer Aktor mit verformbarer Verschlussanordnung und langer Lagerfähigkeit
US20140255275A1 (en) * 2013-03-07 2014-09-11 Quidel Corporation Dual chamber liquid packaging system

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CN106573244A (zh) 2017-04-19
WO2016026718A1 (fr) 2016-02-25
DE102014216391A1 (de) 2016-02-25
US10562029B2 (en) 2020-02-18
CN106573244B (zh) 2020-02-07
EP3183060A1 (fr) 2017-06-28
US20170225168A1 (en) 2017-08-10
ES2719600T3 (es) 2019-07-11

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