EP3083699A1 - Procédé d'immobilisation et de séchage d'enzymes - Google Patents

Procédé d'immobilisation et de séchage d'enzymes

Info

Publication number
EP3083699A1
EP3083699A1 EP14808645.7A EP14808645A EP3083699A1 EP 3083699 A1 EP3083699 A1 EP 3083699A1 EP 14808645 A EP14808645 A EP 14808645A EP 3083699 A1 EP3083699 A1 EP 3083699A1
Authority
EP
European Patent Office
Prior art keywords
dryer
protein
carrier
drying
enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP14808645.7A
Other languages
German (de)
English (en)
Inventor
Steffen Maurer
Robert Bayer
Michael Budde
Michael Kerber
Farzad FARIVAR-MEMAR
Jürgen Däuwel
Thomas Berg
Sascha ROLLIE
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF SE
Original Assignee
BASF SE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BASF SE filed Critical BASF SE
Priority to EP14808645.7A priority Critical patent/EP3083699A1/fr
Publication of EP3083699A1 publication Critical patent/EP3083699A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/087—Acrylic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01003—Triacylglycerol lipase (3.1.1.3)

Definitions

  • CALB-derived lipases are lipases that have at least one, preferably two or more, amino acid changes, such as insertions, deletions or substitutions, to the CALB polypeptide sequence.
  • Suitable carriers are various organic or inorganic materials such as silica gel, activated carbon or polymer carrier.
  • macroporous crosslinked polymers having a particle size of 100 to 1000 ⁇ m and an average pore radius of 10 to 20 nm are suitable.
  • macroporous crosslinked acrylate polymers such as, for example, poly (meth) acrylates which are crosslinked with divinylbenzene, which may comprise, for example, acrylic acid, acrylic acid esters, methacrylic acid and methacrylic acid esters.
  • Such polymers are sold, for example, by the company Lanxess under the name Lewatit® VP OC 1600 or by the company DOW under the name Amberlite® XAD-7.
  • TBU Total volatilestrogenicity
  • US [%] [g / L] protein
  • the infra-red balance was used in a triple measurement to determine the mean dry content of the sample taken from the filled big-bag.
  • the measurements gave the following result (infrared balance, end point: 30 sec. Balance standstill, triple measurement: 5.3 g / 9.3 g / 4.6 g):
  • the mean dry content of the bottled product is 99.1 1%. This in turn corresponds to a residual moisture content of 0.89%.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Procédé d'immobilisation de protéines sur un support, caractérisé en ce que l'incubation de la protéine avec le support dans une phase aqueuse a lieu dans un mélangeur-sécheur sous vide par contact à fonctionnement discontinu et le séchage de la protéine immobilisée est réalisé immédiatement après dans le même mélangeur-sécheur sous vide par contact, éventuellement après une étape de lavage facultative.
EP14808645.7A 2013-12-16 2014-12-08 Procédé d'immobilisation et de séchage d'enzymes Withdrawn EP3083699A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP14808645.7A EP3083699A1 (fr) 2013-12-16 2014-12-08 Procédé d'immobilisation et de séchage d'enzymes

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP13197469 2013-12-16
PCT/EP2014/076844 WO2015091046A1 (fr) 2013-12-16 2014-12-08 Procédé d'immobilisation et de séchage d'enzymes
EP14808645.7A EP3083699A1 (fr) 2013-12-16 2014-12-08 Procédé d'immobilisation et de séchage d'enzymes

Publications (1)

Publication Number Publication Date
EP3083699A1 true EP3083699A1 (fr) 2016-10-26

Family

ID=49765918

Family Applications (1)

Application Number Title Priority Date Filing Date
EP14808645.7A Withdrawn EP3083699A1 (fr) 2013-12-16 2014-12-08 Procédé d'immobilisation et de séchage d'enzymes

Country Status (6)

Country Link
US (1) US20160312209A1 (fr)
EP (1) EP3083699A1 (fr)
JP (1) JP2016540515A (fr)
CN (1) CN105849130A (fr)
CA (1) CA2933661A1 (fr)
WO (1) WO2015091046A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021127479A1 (fr) * 2019-12-18 2021-06-24 Danisco Us Inc Stabilisation d'oxydases par séchage sous pression partielle réduite d'oxygène

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3666627A (en) * 1968-10-14 1972-05-30 Corning Glass Works Method of stabilizing enzymes
US3708397A (en) * 1969-12-22 1973-01-02 Baxter Laboratories Inc Syrup conversion with immobilized glucose isomerase
WO1989002916A1 (fr) * 1987-09-28 1989-04-06 Novo-Nordisk A/S Procede d'immobilisation de lipase
DK2245146T3 (en) 2007-12-20 2015-06-29 Basf Se NEW CALB muteins AND THEIR USE

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
None *
See also references of WO2015091046A1 *

Also Published As

Publication number Publication date
US20160312209A1 (en) 2016-10-27
JP2016540515A (ja) 2016-12-28
CA2933661A1 (fr) 2015-06-25
WO2015091046A1 (fr) 2015-06-25
CN105849130A (zh) 2016-08-10

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