EP2735608B1 - Composite de c'ur-écorce ayant du poly-vinylalcool et de l'alginate et son procédé de fabrication - Google Patents

Composite de c'ur-écorce ayant du poly-vinylalcool et de l'alginate et son procédé de fabrication Download PDF

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Publication number
EP2735608B1
EP2735608B1 EP13165156.4A EP13165156A EP2735608B1 EP 2735608 B1 EP2735608 B1 EP 2735608B1 EP 13165156 A EP13165156 A EP 13165156A EP 2735608 B1 EP2735608 B1 EP 2735608B1
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EP
European Patent Office
Prior art keywords
beads
core
reactant
pva
shell
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Not-in-force
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EP13165156.4A
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German (de)
English (en)
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EP2735608A1 (fr
Inventor
Hyokwan Bae
Seockheon Lee
Yunchul Chung
Jin-Young Jung
Hee Jeong Yang
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Korea Advanced Institute of Science and Technology KAIST
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Korea Advanced Institute of Science and Technology KAIST
Korea Institute of Science and Technology KIST
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J13/00—Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
    • B01J13/02—Making microcapsules or microballoons
    • B01J13/06—Making microcapsules or microballoons by phase separation
    • B01J13/14—Polymerisation; cross-linking
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/02—Aerobic processes
    • C02F3/10—Packings; Fillings; Grids
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/28—Treatment of water, waste water, or sewage by sorption
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/02—Aerobic processes
    • C02F3/10—Packings; Fillings; Grids
    • C02F3/105—Characterized by the chemical composition
    • C02F3/107—Inorganic materials, e.g. sand, silicates
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/02—Aerobic processes
    • C02F3/10—Packings; Fillings; Grids
    • C02F3/105—Characterized by the chemical composition
    • C02F3/108—Immobilising gels, polymers or the like
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/10—Biological treatment of water, waste water, or sewage

Definitions

  • Ca-alginate beads used frequently in the encapsulated immobilization method according to the related art have low mechanical strength and undergo cleavage of their binding structure due to phosphate ions, and thus are limited in practical use.
  • Santos et al. have suggested that a core-shell dual structure is used to provide core-shell structure beads ( V.A.P.M dos Santos et al., Modeling and evaluation of an integrated nitrogen removal system with microorganisms co-immobilization in double-layer gel beads, Biotechnology Progress, 1996, 12, 240-248 ).
  • a core-shell structure composite carrier based on poly(vinyl alcohol) (PVA) and alginate. Both the core region and the shell region are formed from beads of PVA and alginate. After a spherical core region is formed, a shell region is formed on the surface of the core region. Since the composite carrier has a dual structure of a core region and a shell region and uses a synthetic polymeric material, PVA, it ensures high mechanical strength.
  • PVA poly(vinyl alcohol)
  • the beads of a core region are immersed into a saturated boric acid (H 3 BO 3 ) solution containing 0.5-1 w/v% of calcium chloride (CaCl 2 ) to form a crosslinking agent on the surface of the beads of a core region (S104).
  • a saturated boric acid H 3 BO 3
  • calcium chloride functions as a crosslinking agent for alginate
  • a saturated boric acid functions as a crosslinking agent for PVA.
  • the crosslinking agents are formed on the surface of the beads of a core region.
  • the amount of crosslinking agents is in proportion to the immersion time.
  • the amount of crosslinking agents is also in proportion to the thickness of beads.
  • the thickness of the beads of a shell region increases.
  • the thickness of the beads of a shell region increases above a predetermined value, it is possible to realize a gradient in dissolved oxygen concentration depending on the distance between the shell region and the core region. By using this, it is possible to provide aerobic microorganisms and anaerobic microorganisms to the shell region and the core region, respectively. Meanwhile, it can be seen from Fig. 4 that the thickness of the beads of a shell region increases in proportion to the immersion time.
  • the mixed solution for a shell is a mixture of a PVA-alginate mixed solution in combination with a first reactant-containing mixed solution
  • the PVA-alginate mixed solution is a solution containing 10-20 w/v% of PVA in combination with 1-5 w/v% of sodium alginate dissolved in distilled water.
  • the first reactant-containing mixed solution is a solution containing the first reactant.
  • the first reactant is a material to be immobilized to the shell region, i.e.
  • the selective medium maintains (NH 4 ) 2 SO 4 and NaNO 2 that are main energy sources for anaerobic ammonium oxidizing microorganisms at the same concentration of 50 mg/L in terms of nitrogen, and is prepared from NaCO 3 -C 350 mg/L, KH 2 PO 4 -P 6 mg/L, MgSO 4 •H 2 O-Mg 12 mg/L, CaCl 2 •2H 2 O-Ca 48 mg/L, trace element I solution (EDTA 5 g/L and FeSO 4 •7H 2 O 5 g/L) 1ml/L, trace element II solution (ZnSO 4 •7H 2 O 0.43 g/L, CoCl 2 •6H 2 O 0.24 g/L, MnCl 2 •4H 2 O 0.99 g/L, CuSO 4 •5H 2 O 0.25 g/L, Na 2 MoO 4 •2H 2 O 0.22 g/L, NiCl 2 •6H 2 O 0.19 g/L, Na 2 SeO 4

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Water Supply & Treatment (AREA)
  • Hydrology & Water Resources (AREA)
  • Environmental & Geological Engineering (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Inorganic Chemistry (AREA)
  • Materials Engineering (AREA)
  • Dispersion Chemistry (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Biological Treatment Of Waste Water (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Claims (14)

  1. Procédé de préparation d'un support composite ayant une structure noyau-enveloppe à base d'alcool polyvinylique (APV) et d'alginate, comprenant :
    fournir une solution mixte pour un noyau contenant de l'APV, de l'alginate et un deuxième réactif ;
    introduire la solution mixte pour un noyau dans une solution d'acide borique contenant du chlorure de calcium afin de former des billes d'une région de noyau sur lesquelles le deuxième réactif est immobilisé ;
    immerger les billes d'une région de noyau dans une solution d'acide borique contenant du chlorure de calcium afin de former un agent de réticulation sur les billes d'une région de noyau ; et
    introduire les billes d'une région de noyau ayant l'agent de réticulation dans une solution mixte pour une enveloppe afin de former des billes d'une région d'enveloppe sur la surface des billes d'une région de noyau,
    dans lequel la solution mixte pour une enveloppe comprend l'APV, l'alginate et un premier réactif.
  2. Procédé de préparation d'un support composite ayant une structure noyau-enveloppe à base d'APV et d'alginate selon la revendication 1, dans lequel la solution mixte pour un noyau est un mélange d'une solution mixte APV-alginate en combinaison avec une solution mixte contenant un deuxième réactif, la solution mixte APV-alginate est une solution contenant 10-20 w/v% de APV en combinaison avec 1-5 w/v% d'alginate de sodium dissous dans l'eau distillée, et la solution mixte contenant un deuxième réactif est une solution qui contient le deuxième réactif.
  3. Procédé de préparation d'un support composite ayant une structure noyau-enveloppe à base d'APV et d'alginate selon la revendication 1, dans lequel la solution mixte pour une enveloppe est un mélange d'une solution mixte APV-alginate en combinaison avec une solution mixte contenant un premier réactif, la solution mixte APV-alginate est une solution contenant 10-20 w/v% d'APV en combinaison avec 1-5 w/v% d'alginate de sodium dissous dans l'eau distillée, et la solution mixte contenant un premier réactif est une solution qui contient le premier réactif.
  4. Procédé de préparation d'un support composite ayant une structure noyau-enveloppe à base d'APV et d'alginate selon l'une quelconque des revendications 1 à 3, dans lequel le premier réactif est un micro-organisme aérobie nitrifiant et le deuxième réactif est un micro-organisme anaérobie oxydant l'ammoniac.
  5. Procédé de préparation d'un support composite ayant une structure noyau-enveloppe à base d'APV et d'alginate selon l'une quelconque des revendications 1 à 3, dans lequel le premier réactif est le fer à valence zéro par lequel le nitrate est converti en ammoniac et le deuxième réactif est un adsorbant solide sur lequel l'ammoniac est adsorbé.
  6. Procédé de préparation d'un support composite ayant une structure noyau-enveloppe à base d'APV et d'alginate selon l'une quelconque des revendications 1 à 3, dans lequel le premier réactif est un micro-organisme anaérobie pour enlever une substance organique toxique et le deuxième réactif est un micro-organisme anaérobie.
  7. Procédé de préparation d'un support composite ayant une structure noyau-enveloppe à base d'APV et d'alginate selon l'une quelconque des revendications 1 à 6 qui comprend en outre, après ladite formation des billes d'une région de noyau et après ladite formation des billes d'une région d'enveloppe, immerger les billes finies d'une région de noyau ou celles d'une région d'enveloppe dans une solution d'acide phosphorique afin de renforcer la résistance mécanique des billes.
  8. Procédé de préparation d'un support composite ayant une structure noyau-enveloppe à base d'APV et d'alginate selon l'une quelconque des revendications 1 à 7, dans lequel la solution mixte pour un noyau est introduite au moyen d'un quelconque choisi parmi une aiguille, un tube et un entonnoir afin de contrôler la taille des billes d'une région de noyau lors de la formation des billes d'une région de noyau.
  9. Procédé de préparation d'un support composite ayant une structure noyau-enveloppe à base d'APV et d'alginate selon l'une quelconque des revendications 1 à 8, dans lequel la durée pendant laquelle les billes d'une région de noyau sont immergées dans une solution d'acide borique est contrôlée afin de contrôler l'épaisseur des billes d'une région d'enveloppe lors de l'immersion des billes d'une région de noyau dans une solution d'acide borique contenant du chlorure de calcium afin de former un agent de réticulation sur les billes d'une région de noyau.
  10. Procédé de préparation d'un support composite ayant une structure noyau-enveloppe à base d'APV et d'alginate selon l'une quelconque des revendications 1 à 8, dans lequel la durée pendant laquelle les billes d'une région de noyau ayant l'agent de réticulation sont introduites dans la solution mixte pour une enveloppe est contrôlée afin de contrôler l'épaisseur des billes d'une région d'enveloppe lors de l'immersion des billes d'une région de noyau ayant l'agent de réticulation dans une solution mixte pour une enveloppe afin de former des billes d'une région d'enveloppe sur la surface des billes d'une région de noyau.
  11. Support composite ayant une structure noyau-enveloppe à base d'alcool polyvinylique (APV) et d'alginate comprenant des billes d'une région de noyau et des billes d'une région d'enveloppe formées sur la surfaces des billes d'une région de noyau, dans lequel un premier réactif effectuant une première réaction unique est immobilisé sur les billes d'une région d'enveloppe, un deuxième réactif effectuant une deuxième réaction unique est immobilisé sur les billes d'une région de noyau, la première réaction unique et la deuxième réaction unique sont indépendantes l'une de l'autre et sont des réactions continues successives dans le temps, et les billes d'une région de noyau et les billes d'une région d'enveloppe comprennent l'APV et l'alginate.
  12. Support composite ayant une structure noyau-enveloppe à base d'APV et d'alginate selon la revendication 11, dans lequel le premier réactif est un micro-organisme aérobie nitrifiant et le deuxième réactif est un micro-organisme anaérobie oxydant l'ammoniac.
  13. Support composite ayant une structure noyau-enveloppe à base d'APV et d'alginate selon la revendication 11, dans lequel le premier réactif est le fer à valence zéro par lequel le nitrate est converti en ammoniac et le deuxième réactif est un adsorbant solide sur lequel l'ammoniac est adsorbé.
  14. Support composite ayant une structure noyau-enveloppe à base d'APV et d'alginate selon la revendication 11, dans lequel le premier réactif est un micro-organisme anaérobie pour enlever une substance organique toxique et le deuxième réactif est un micro-organisme anaérobie.
EP13165156.4A 2012-11-26 2013-04-24 Composite de c'ur-écorce ayant du poly-vinylalcool et de l'alginate et son procédé de fabrication Not-in-force EP2735608B1 (fr)

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KR20120134310A KR101484608B1 (ko) 2012-11-26 2012-11-26 Pva와 알지네이트 기반 코어-쉘 구조의 복합담체 및 그 제조방법

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CN110342640A (zh) * 2019-08-12 2019-10-18 苏州方元生态科技有限公司 包埋装置及固定化微生物载体的制作方法
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CN105884026A (zh) * 2016-04-27 2016-08-24 罗璐 一种核壳式结构的微生物载体及浮沉式治理污染水体的方法
CN105884026B (zh) * 2016-04-27 2019-04-02 北京清源中科环保科技有限公司 一种核壳式结构的微生物载体及浮沉式治理污染水体的方法

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DK2735608T3 (en) 2015-11-02
KR20140067271A (ko) 2014-06-05
KR101484608B1 (ko) 2015-01-22

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