EP2707692A1 - Guidage de cellules fluidique pour cytométrie en flux - Google Patents

Guidage de cellules fluidique pour cytométrie en flux

Info

Publication number
EP2707692A1
EP2707692A1 EP12748175.2A EP12748175A EP2707692A1 EP 2707692 A1 EP2707692 A1 EP 2707692A1 EP 12748175 A EP12748175 A EP 12748175A EP 2707692 A1 EP2707692 A1 EP 2707692A1
Authority
EP
European Patent Office
Prior art keywords
channel
flow
guide
cells
channel bottom
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP12748175.2A
Other languages
German (de)
English (en)
Inventor
Oliver Hayden
Michael Johannes HELOU
Sandro Francesco Tedde
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens AG
Original Assignee
Siemens AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Siemens AG filed Critical Siemens AG
Publication of EP2707692A1 publication Critical patent/EP2707692A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects thereof, e.g. conductivity or capacity
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502776—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for focusing or laminating flows
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Fluid Mechanics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Optical Measuring Cells (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un dispositif et un procédé de guidage de cellules fluidique pour cytométrie en flux ou enrichissement d'analytes. Des analytes marqués par voie magnétique, notamment des cellules (1), peuvent être enrichis de manière dynamique dans le flux à partir d'un échantillon et détectés individuellement, en particulier par détection magnétorésistive. Pour le guidage cellulaire, des pièces de guidage (12) sont disposées dans un canal d'écoulement (100) de manière à ce qu'une force de déviation (10x) supplémentaire agisse par les obstacles fluidiques (12) sur les cellules à détecter, en plus d'une force d'enrichissement magnétique (10z) et de la force de cisaillement du flux (10y).
EP12748175.2A 2011-07-28 2012-07-24 Guidage de cellules fluidique pour cytométrie en flux Withdrawn EP2707692A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102011080012.3A DE102011080012B4 (de) 2011-07-28 2011-07-28 Strömungsmechanische Zellführung für Durchflusszytometrie
PCT/EP2012/064470 WO2013014146A1 (fr) 2011-07-28 2012-07-24 Guidage de cellules fluidique pour cytométrie en flux

Publications (1)

Publication Number Publication Date
EP2707692A1 true EP2707692A1 (fr) 2014-03-19

Family

ID=46690476

Family Applications (1)

Application Number Title Priority Date Filing Date
EP12748175.2A Withdrawn EP2707692A1 (fr) 2011-07-28 2012-07-24 Guidage de cellules fluidique pour cytométrie en flux

Country Status (6)

Country Link
US (1) US9651470B2 (fr)
EP (1) EP2707692A1 (fr)
CN (1) CN103718019A (fr)
CA (1) CA2843098A1 (fr)
DE (1) DE102011080012B4 (fr)
WO (1) WO2013014146A1 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102011080012B4 (de) 2011-07-28 2015-09-10 Siemens Aktiengesellschaft Strömungsmechanische Zellführung für Durchflusszytometrie
DE102012210457B4 (de) * 2012-06-21 2015-08-27 Siemens Aktiengesellschaft Verfahren und Anordnung zur partiellen Markierung und anschließenden Quantifizierung von Zellen einer Zellsuspension
US20150107711A1 (en) * 2013-10-21 2015-04-23 Cytoflow, Llc Cytometer flowcell
DE102015218177B4 (de) 2015-09-22 2022-09-01 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Isolation und Anreicherung magnetisch markierter Zellen im Durchfluss
JP7170047B2 (ja) * 2017-12-21 2022-11-11 ラジオメーター・メディカル・アー・ペー・エス 流体試料を収容するためのデバイス

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6736978B1 (en) * 2000-12-13 2004-05-18 Iowa State University Research Foundation, Inc. Method and apparatus for magnetoresistive monitoring of analytes in flow streams
US20110059500A1 (en) * 2009-09-10 2011-03-10 National Taiwan University Method for separating immunomagnetic bead labeled particulates

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030095897A1 (en) 2001-08-31 2003-05-22 Grate Jay W. Flow-controlled magnetic particle manipulation
US6976590B2 (en) * 2002-06-24 2005-12-20 Cytonome, Inc. Method and apparatus for sorting particles
US8361413B2 (en) * 2005-06-09 2013-01-29 The United States Of America, As Represented By The Secretary Of The Navy Sheath flow device
US9220831B2 (en) * 2005-10-06 2015-12-29 Children's Medical Center Corporation Device and method for combined microfluidic-micromagnetic separation of material in continuous flow
US7807454B2 (en) * 2006-10-18 2010-10-05 The Regents Of The University Of California Microfluidic magnetophoretic device and methods for using the same
US8409877B2 (en) * 2006-12-29 2013-04-02 Intel Corporation Enzymatic signal generation and detection of binding complexes in stationary fluidic chip
US8551333B2 (en) * 2007-04-05 2013-10-08 The Regents Of The University Of California Particle-based microfluidic device for providing high magnetic field gradients
WO2009132151A2 (fr) * 2008-04-23 2009-10-29 The Regents Of The University Of California Dispositifs microfluidiques et leurs procédés d’utilisation
CN102713640B (zh) 2009-06-10 2015-09-16 辛温尼奥生物系统公司 鞘流装置和方法
DE102011080012B4 (de) 2011-07-28 2015-09-10 Siemens Aktiengesellschaft Strömungsmechanische Zellführung für Durchflusszytometrie

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6736978B1 (en) * 2000-12-13 2004-05-18 Iowa State University Research Foundation, Inc. Method and apparatus for magnetoresistive monitoring of analytes in flow streams
US20110059500A1 (en) * 2009-09-10 2011-03-10 National Taiwan University Method for separating immunomagnetic bead labeled particulates

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO2013014146A1 *

Also Published As

Publication number Publication date
CN103718019A (zh) 2014-04-09
CA2843098A1 (fr) 2013-01-31
DE102011080012A1 (de) 2013-01-31
DE102011080012B4 (de) 2015-09-10
WO2013014146A1 (fr) 2013-01-31
US9651470B2 (en) 2017-05-16
US20140159714A1 (en) 2014-06-12

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