EP2623591A3 - Geobacillus stearothermophilus alpha-amylase (AMYS) variants with improved properties - Google Patents

Geobacillus stearothermophilus alpha-amylase (AMYS) variants with improved properties Download PDF

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Publication number
EP2623591A3
EP2623591A3 EP20130165079 EP13165079A EP2623591A3 EP 2623591 A3 EP2623591 A3 EP 2623591A3 EP 20130165079 EP20130165079 EP 20130165079 EP 13165079 A EP13165079 A EP 13165079A EP 2623591 A3 EP2623591 A3 EP 2623591A3
Authority
EP
European Patent Office
Prior art keywords
amylase
variants
amys
improved properties
geobacillus stearothermophilus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP20130165079
Other languages
German (de)
French (fr)
Other versions
EP2623591B1 (en
EP2623591A2 (en
Inventor
William Cuevas
David Estell
Sura Hussain Hadi
Sang-Kyu Lee
Sandra Ramer
Andrew Shaw
Amr Toppozada
Walter Weyler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Danisco US Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=41100457&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EP2623591(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Danisco US Inc filed Critical Danisco US Inc
Publication of EP2623591A2 publication Critical patent/EP2623591A2/en
Publication of EP2623591A3 publication Critical patent/EP2623591A3/en
Application granted granted Critical
Publication of EP2623591B1 publication Critical patent/EP2623591B1/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M16/00—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
    • D06M16/003—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic with enzymes or microorganisms
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38609—Protease or amylase in solid compositions only
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38618—Protease or amylase in liquid compositions only
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/3869—Enzyme enhancers or mediators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001—Alpha-amylase (3.2.1.1)
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06L—DRY-CLEANING, WASHING OR BLEACHING FIBRES, FILAMENTS, THREADS, YARNS, FABRICS, FEATHERS OR MADE-UP FIBROUS GOODS; BLEACHING LEATHER OR FURS
    • D06L4/00—Bleaching fibres, filaments, threads, yarns, fabrics, feathers or made-up fibrous goods; Bleaching leather or furs
    • D06L4/40—Bleaching fibres, filaments, threads, yarns, fabrics, feathers or made-up fibrous goods; Bleaching leather or furs using enzymes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Textile Engineering (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Described are variants of a parent alpha-amylase that exhibits an alteration in at least one of the following properties relative to said parent alpha-amylase: specific activity, substrate specificity, substrate binding, substrate cleavage, thermal stability, pH-dependent activity, pH-dependent stability, oxidative stability, calcium dependency, pI, and wash performance. The variants are suitable for starch conversion, ethanol production, laundry washing, dish washing, hard surface cleaning, textile desizing, and/or sweetener production.
EP13165079.8A 2008-06-06 2009-06-02 Geobacillus stearothermophilus alpha-amylase (AMYS) variants with improved properties Active EP2623591B1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US5942308P 2008-06-06 2008-06-06
EP09759289A EP2300605A2 (en) 2008-06-06 2009-06-02 Geobacillus stearothermophilus alpha-amylase (amys) variants with improved properties
EP11187405.3A EP2447361B1 (en) 2008-06-06 2009-06-02 Geobacillus stearothermophilus alpha-amylase (AMYS) variants with improved properties

Related Parent Applications (5)

Application Number Title Priority Date Filing Date
EP11187405.3A Division EP2447361B1 (en) 2008-06-06 2009-06-02 Geobacillus stearothermophilus alpha-amylase (AMYS) variants with improved properties
EP11187405.3A Division-Into EP2447361B1 (en) 2008-06-06 2009-06-02 Geobacillus stearothermophilus alpha-amylase (AMYS) variants with improved properties
EP09759289.3 Division 2009-06-02
EP09759289A Division EP2300605A2 (en) 2008-06-06 2009-06-02 Geobacillus stearothermophilus alpha-amylase (amys) variants with improved properties
EP11187405.3 Division 2011-11-01

Publications (3)

Publication Number Publication Date
EP2623591A2 EP2623591A2 (en) 2013-08-07
EP2623591A3 true EP2623591A3 (en) 2013-11-20
EP2623591B1 EP2623591B1 (en) 2016-08-24

Family

ID=41100457

Family Applications (3)

Application Number Title Priority Date Filing Date
EP11187405.3A Active EP2447361B1 (en) 2008-06-06 2009-06-02 Geobacillus stearothermophilus alpha-amylase (AMYS) variants with improved properties
EP09759289A Withdrawn EP2300605A2 (en) 2008-06-06 2009-06-02 Geobacillus stearothermophilus alpha-amylase (amys) variants with improved properties
EP13165079.8A Active EP2623591B1 (en) 2008-06-06 2009-06-02 Geobacillus stearothermophilus alpha-amylase (AMYS) variants with improved properties

Family Applications Before (2)

Application Number Title Priority Date Filing Date
EP11187405.3A Active EP2447361B1 (en) 2008-06-06 2009-06-02 Geobacillus stearothermophilus alpha-amylase (AMYS) variants with improved properties
EP09759289A Withdrawn EP2300605A2 (en) 2008-06-06 2009-06-02 Geobacillus stearothermophilus alpha-amylase (amys) variants with improved properties

Country Status (12)

Country Link
US (2) US8084240B2 (en)
EP (3) EP2447361B1 (en)
JP (1) JP5492879B2 (en)
CN (2) CN105483099B (en)
AU (2) AU2009256280B2 (en)
BR (1) BRPI0913402B1 (en)
CA (1) CA2726265A1 (en)
DK (1) DK2447361T3 (en)
ES (1) ES2527645T3 (en)
MX (1) MX2010013113A (en)
PL (1) PL2447361T3 (en)
WO (1) WO2009149130A2 (en)

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