EP2526199A4 - Procédés de production de nucléases en doigt de zinc ayant une activité modifiée - Google Patents

Procédés de production de nucléases en doigt de zinc ayant une activité modifiée

Info

Publication number
EP2526199A4
EP2526199A4 EP11735277.3A EP11735277A EP2526199A4 EP 2526199 A4 EP2526199 A4 EP 2526199A4 EP 11735277 A EP11735277 A EP 11735277A EP 2526199 A4 EP2526199 A4 EP 2526199A4
Authority
EP
European Patent Office
Prior art keywords
methods
zinc finger
catalytic activity
zfn
altered
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP11735277.3A
Other languages
German (de)
English (en)
Other versions
EP2526199A2 (fr
Inventor
Iii Carlos F Barbas
Jing Guo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Scripps Research Institute
Original Assignee
Scripps Research Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripps Research Institute filed Critical Scripps Research Institute
Publication of EP2526199A2 publication Critical patent/EP2526199A2/fr
Publication of EP2526199A4 publication Critical patent/EP2526199A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C07K14/4703—Inhibitors; Suppressors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
    • G01N2333/922—Ribonucleases (RNAses); Deoxyribonucleases (DNAses)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
EP11735277.3A 2010-01-22 2011-01-21 Procédés de production de nucléases en doigt de zinc ayant une activité modifiée Withdrawn EP2526199A4 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US29759810P 2010-01-22 2010-01-22
US32903510P 2010-04-28 2010-04-28
PCT/US2011/022154 WO2011091324A2 (fr) 2010-01-22 2011-01-21 Procédés de production de nucléases en doigt de zinc ayant une activité modifiée

Publications (2)

Publication Number Publication Date
EP2526199A2 EP2526199A2 (fr) 2012-11-28
EP2526199A4 true EP2526199A4 (fr) 2013-08-07

Family

ID=44307625

Family Applications (1)

Application Number Title Priority Date Filing Date
EP11735277.3A Withdrawn EP2526199A4 (fr) 2010-01-22 2011-01-21 Procédés de production de nucléases en doigt de zinc ayant une activité modifiée

Country Status (2)

Country Link
EP (1) EP2526199A4 (fr)
WO (1) WO2011091324A2 (fr)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102660642A (zh) * 2012-04-24 2012-09-12 西北农林科技大学 一种筛选锌指蛋白的筛选系统
US20180156807A1 (en) 2015-04-29 2018-06-07 New York University Method for treating high-grade gliomas
WO2018183766A1 (fr) * 2017-03-29 2018-10-04 Editas Medicine, Inc. Méthodes de sélection
JP2020524497A (ja) 2017-06-09 2020-08-20 エディタス・メディシン，インコーポレイテッド 操作されたｃａｓ９ヌクレアーゼ
CN108118059B (zh) * 2017-12-30 2021-03-19 苏州金唯智生物科技有限公司 一种改进的启动子及其组成的载体和应用
WO2021048291A1 (fr) * 2019-09-12 2021-03-18 Glaxosmithkline Intellectual Property Development Limited Procédé de criblage de bibliothèques
JP2023524666A (ja) 2020-04-28 2023-06-13 インテリア セラピューティクス，インコーポレイテッド インビトロ細胞送達の方法
CN116406383A (zh) * 2020-11-06 2023-07-07 日商基因编辑力股份有限公司 FokI核酸酶结构域的突变体
JP2024501246A (ja) 2020-12-23 2024-01-11 インテリア セラピューティクス，インコーポレイテッド 細胞中のｃｉｉｔａを遺伝子修飾するための組成物及び方法
JP2024500858A (ja) 2020-12-23 2024-01-10 インテリア セラピューティクス，インコーポレイテッド 細胞中のｈｌａ－ａを低減するための組成物及び方法
KR20230153356A (ko) 2020-12-30 2023-11-06 인텔리아 테라퓨틱스, 인크. 조작된 t 세포
WO2022221696A1 (fr) 2021-04-17 2022-10-20 Intellia Therapeutics, Inc. Inhibiteurs de protéine kinase dépendante de l'adn, et compositions et utilisations de ceux-ci
WO2022221695A1 (fr) 2021-04-17 2022-10-20 Intellia Therapeutics, Inc. Compositions de nanoparticules lipidiques
BR112023021477A2 (pt) 2021-04-17 2024-01-30 Intellia Therapeutics Inc Composições de nanopartículas lipídicas
WO2023205148A1 (fr) 2022-04-19 2023-10-26 Intellia Therapeutics, Inc. Compositions de récepteurs antigéniques chimériques et utilisations
TW202409271A (zh) 2022-06-16 2024-03-01 美商英特利亞醫療公司 用於減少細胞中之ｍｈｃ １類之組成物及方法
WO2024006955A1 (fr) 2022-06-29 2024-01-04 Intellia Therapeutics, Inc. Lymphocytes t modifiés

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007139898A2 (fr) * 2006-05-25 2007-12-06 Sangamo Biosciences, Inc. Demi-domaines résultant de clivage par génie génétique

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE347593T1 (de) * 2002-01-23 2006-12-15 Univ Utah Res Found Zielgerichtete chromosomale mutagenese mit zinkfingernukleasen
WO2006121703A2 (fr) * 2005-05-06 2006-11-16 The Board Of Trustees Of The University Of Illinois Cartographie de nouveaux sites pour action antibiotique dans le ribosome
EP2167666A2 (fr) * 2007-06-29 2010-03-31 Pioneer Hi-Bred International Inc. Procédés de modification du génome d'une cellule de plante monocotylédone

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007139898A2 (fr) * 2006-05-25 2007-12-06 Sangamo Biosciences, Inc. Demi-domaines résultant de clivage par génie génétique

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
"Structure-Based Engineering of the FokI DNA Cleavage Domain in the Context of a Chimeric Endonuclease: Towards Maximally Efficient ''Gene Editing'' Therapy", MOLECULAR THERAPY, NATURE PUBLISHING GROUP, GB, vol. 9, 1 May 2004 (2004-05-01), pages 272, XP004635080, ISSN: 1525-0016 *
JING GUO ET AL: "Directed Evolution of an Enhanced and Highly Efficient FokI Cleavage Domain for Zinc Finger Nucleases", JOURNAL OF MOLECULAR BIOLOGY, vol. 400, no. 1, 4 May 2010 (2010-05-04), pages 96 - 107, XP055065197, ISSN: 0022-2836, DOI: 10.1016/j.jmb.2010.04.060 *
MANI M ET AL: "Design, engineering, and characterization of zinc finger nucleases", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 335, no. 2, 23 September 2005 (2005-09-23), pages 447 - 457, XP027230194, ISSN: 0006-291X, [retrieved on 20050811], DOI: 10.1016/J.BBRC.2005.07.089 *
URNOV F D ET AL: "Highly efficient endogenous human gene correction using designed zinc-finger nucleases", NATURE: INTERNATIONAL WEEKLY JOURNAL OF SCIENCE, NATURE PUBLISHING GROUP, UNITED KINGDOM, vol. 435, no. 7042, 2 June 2005 (2005-06-02), pages 646 - 651, XP002411069, ISSN: 0028-0836, DOI: 10.1038/NATURE03556 *

Also Published As

Publication number Publication date
WO2011091324A8 (fr) 2012-08-30
WO2011091324A3 (fr) 2011-12-22
EP2526199A2 (fr) 2012-11-28
WO2011091324A2 (fr) 2011-07-28

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