EP2429705B1 - Anordnung aus einer filtrationsmembran und einer trägerplatte - Google Patents

Anordnung aus einer filtrationsmembran und einer trägerplatte Download PDF

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Publication number
EP2429705B1
EP2429705B1 EP10721456.1A EP10721456A EP2429705B1 EP 2429705 B1 EP2429705 B1 EP 2429705B1 EP 10721456 A EP10721456 A EP 10721456A EP 2429705 B1 EP2429705 B1 EP 2429705B1
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EP
European Patent Office
Prior art keywords
membrane
plate
fact
assembly according
plane
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP10721456.1A
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English (en)
French (fr)
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EP2429705A1 (de
Inventor
Morgan Gilet
Géraldine TAINE
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biomerieux SA
Original Assignee
AES Chemunex SA
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Publication of EP2429705A1 publication Critical patent/EP2429705A1/de
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/04—Exchange or ejection of cartridges, containers or reservoirs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter

Definitions

  • the present invention is in the field of microbiological testing equipment for liquid phase products.
  • the detection and analysis of particles, living or not, contained in liquid or gaseous fluids, food, pharmaceutical, biological, cosmetic or hygiene products, as well as the monitoring of fermentation processes comprises in general, a step of depositing the particles on a support and the optical analysis, for example by scanning, of the surface of this support.
  • the present applicant has developed a technique whereby the particles are retained on a filter membrane, after which it is brought into contact with a material capable of reacting with the contaminating particles to mark them, so that the labeling product reacts with the enzymatic activity of the contaminant to create a fluorescent signal.
  • a support plate which generally comprises a cellulose element or pad impregnated with a marking solution of viable microorganisms.
  • the present invention therefore aims to solve the difficulty detailed above by providing means for correctly positioning a membrane on its support plate, so as to prevent the creation of an air bubble between them.
  • a first object of the invention consists of a set of activation and / or microbiological contaminant disclosure consisting of a flat and rigid filtration membrane, and a generally flat plate, receiving and supporting this membrane, in particular for the purpose of activating and / or revealing immobilized contaminants thereon, this support plate comprising retaining and immobilizing means capable of cooperating with the periphery of the membrane.
  • said means comprise at least one non-deformable member which protrudes above the general plane of said plate, and which comprises at least one tab forming a stop, generally perpendicular to said plane, which is extended by a retaining appendage generally parallel to said plane, so that the engagement of said membrane with this member can be done only in a first movement of the membrane substantially parallel to the plane of the plate, followed by a second movement substantially perpendicular to said plane and that the plate ( 5) comprises, in its central part, a reservation (R) for receiving a material for marking or revealing said contaminants.
  • the presence of at least one non-deformable member constitutes an obstacle that opposes the direct engagement of the membrane on the plate in a directional movement generally perpendicular to the plane thereof.
  • membrane By the term “membrane” is meant both a self-supporting membrane, a flexible filtration structure attached to a rigid frame.
  • first movement of the membrane substantially parallel to the plane of the plate it is to be understood that, in carrying out this operation, the membrane occupies a position very close to the plate, parallel to it, or, at the very least, forming with it, an acute angle of not more than a few degrees (10 to 15 ° maximum).
  • the receptacle 1 and the base 2 shown in FIGS. Figures 1 and 2 annexed are part of a microbiological test device.
  • the receptacle 1 is intended to receive the liquid product that is proposed to test. It has, as it were, a dual function of container and funnel.
  • It comprises a plastic bottle 10, closed by a removable cap 11, which is provided with openings for connection to a pump, for example.
  • This container is preferably transparent so that the operator can view the level of liquid that it contains.
  • this lower part is provided with two notches 120 arranged facing each other, whose function will be explained later.
  • the bottom of the container 10 which ensures its connection with the lower part 12, is open at its center to provide a circular passage 13 whose lower side is lined with a seal 130.
  • This receptacle 1 is intended to cooperate with a base 2 forming a base.
  • This base 2 has a generally cylindrical shape and its peripheral outer wall is, as shown in FIG. figure 5 , provided with two extra thicknesses 23 intended to cooperate with the complementary notches 120 of the lower part 12 of the receptacle 1.
  • This base also comprises two tabs 21, elastically deformable, each carrying a finger 210 intended to engage in the openings 121 of the lower part 12 of the receptacle.
  • These legs 21 are integral with the base 2. They are directed parallel to its longitudinal axis and separated from the rest of the base.
  • the base 2 comprises, parallel to and above a bottom wall 22 provided with a discharge orifice 23, a perforated surface formed of a network of grooves. concentric, which constitutes a filtration surface.
  • Such a structure advantageously replaces a sintered glass surface which previously required to be completed by the use of a cellulose filter support.
  • This filtration surface is intended to receive a rigid filtration membrane such as that represented in FIG. figure 4 .
  • This filtration membrane consists of a filtration structure 31 disposed on a rigid peripheral frame 30 of circular shape.
  • This frame 30 consists for example of a molded plastic material, while the structure 31 of the membrane is for example based on polyethylene terephthalate of a usual thickness of the order of 23 microns and variable porosity of 0.1 a few micrometers.
  • the membrane is provided with two diametrically opposed lugs.
  • the lug 32 has the general shape of a "T" with a base 320 which extends in a radial direction, and a transverse bar 321 slightly curved, which extends perpendicularly to this base.
  • the second lug has the shape of a Latin or Greek cross, with a radial base 330, and a transverse branch 331.
  • This membrane is intended to be positioned on the filtration surface of the base 2 described above so that the structure 31 is superimposed on the aforementioned network of concentric grooves.
  • the base 2 comprises a cutout 24 for the engagement of the lugs 32 and 33, respectively. This cut forms at the same time a means of immobilization and foolproofing.
  • test operation then consists of marking and / or revealing these contaminants so that they can be visualized or even counted.
  • This cap essentially comprises a peripheral cylindrical skirt 4 whose outside diameter corresponds, with the clearance, to the outside diameter of the frame 30 of the membrane 3.
  • This peripheral skirt comprises two indentations 41 diametrically opposite one another, directed parallel to the generatrices of the constituent cylinder of the skirt.
  • Each indentation 41 has an enlarged lower portion 420 with convergent edges 420.
  • the spacing between the edges 420 is smaller than the width of the bases 320 and 330 of the lugs 32 and 33 of the membrane.
  • the cap 4 is also provided with two diametrically opposed lugs 44 that protrude upwards above the skirt 40. They are angularly spaced notches 41 of 90 °.
  • a gripping hand 43 extends perpendicularly to the ears 44.
  • This cap 4 is intended to engage on the membrane 3 when it is positioned on the base 2.
  • the convergent edges 420 deviate by elastic deformation and snap elastically on these lugs so as to join.
  • This "release" operation of the membrane is carried out so as to effect its transfer on a receiving and support plate which appears in particular to figures 7 and 8 .
  • This plate is intended to allow the marking and / or the revelation of the contaminants carried by the membrane 3.
  • This support plate 5 full that is to say not perforated, is a molded plastic plate, generally flat, median plane referenced P to the figure 9 .
  • This plate is provided with an appendix 53, which is in the plane P above and which is in one piece with the rest of the plate.
  • This appendix 53 comprises, in its zone of attachment to the remainder of the plate, a line of mechanical weakening 530, materialized by a groove whose function will be explained later.
  • the plate comprises on its upper surface, integrally molded, a central circular wall 50 whose diameter is equal to the inner diameter of the frame 30 of the membrane 3.
  • This reservation R is intended to accommodate a material such as a cellulose pad impregnated with a material capable of marking or revealing the contaminants carried by the membrane.
  • the plate 5 also comprises other walls 51, which are formed of sections, and which materialize the edge of the plate.
  • the plate 5 comprises means for retaining and immobilizing the membrane 3.
  • These means comprise a non-deformable member 6, which is positioned opposite and opposite the appendix 53.
  • This member 6 consists of a tab 60 forming a stop, which extends from the plate 5 vertically upwards, in a direction generally perpendicular to the plane P.
  • This tab extends, parallel to the plane P, by three horizontal appendages 61.
  • the aforementioned appendix 53 comprises a second member 7 forming a locking member, which also projects above the general plane and the plate, this member 7 being elastically deformable.
  • this member 7 has the shape of a hook, with a foot 70 perpendicular to said plane P, and a locking pin 71, generally parallel to this plane P.
  • the installation of the membrane on the support is done as follows.
  • the lug 32 of the membrane 3 is positioned in a movement substantially parallel to the plane of the plate 5 and represented by the arrow k at the figure 9 .
  • the axis formed by the membrane 3 and the plane P is an acute angle ⁇ of a few degrees.
  • the membrane 3 occupies the position shown in dashed lines at the figure 10 .

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Claims (10)

  1. Anordnung für die Aktivierung und/oder den Nachweis von mikrobiologischen Verunreinigungen, gebildet aus einer ebenen und steifen Filtrationsmembran (3) und einer allgemein ebenen Aufnahme- und Trägerplatte (5) dieser Membran (3), vor allem im Hinblick auf die Aktivierung und/oder den Nachweis von auf dieser blockierten Verunreinigungen, wobei diese Trägerplatte (5) Rückhalte- und Blockademittel aufweist, die imstande sind, mit der Peripherie der Membran (3) zusammenzuwirken, dadurch gekennzeichnet, dass die Mittel mindestens ein nicht verformbares Organ (6) umfassen, das über die allgemeine Ebene der Platte (P) hinausragt und das mindestens ein Bein (60) aufweist, das einen Anschlag bildet, das im Allgemeinen senkrecht zu der Ebene (P) ist, das von einem im Allgemeinen zu der Ebene parallelen Rückhalteansatz verlängert ist, so dass das Eingreifen der Membran (3) in dieses Organ (6) nur gemäß einer ersten Bewegung (k) der Membran etwa parallel zur Ebene (P) der Platte, gefolgt von einer zweiten Bewegung (1) etwa senkrecht zu der Ebene (P), erfolgen kann, und dass die Platte (5) in ihrem mittigen Teil eine Empfangsaussparung (R) eines Markierungs- oder Nachweismaterials der Verunreinigungen aufweist.
  2. Anordnung nach Anspruch 1, dadurch gekennzeichnet, dass sie mindestens ein zweites Organ (7) umfasst, das ein Verriegelungsorgan bildet, das ebenfalls über die allgemeine Ebene (P) der Platte (5) hinausragt, wobei dieses Organ (7) derart elastisch verformbar ist, dass es sich bei der zweiten Bewegung (1) einzieht und dann wieder seine Ausgangsstellung einnimmt, um somit die Membran (3) zu verriegeln.
  3. Anordnung nach Anspruch 2, dadurch gekennzeichnet, dass sich das nicht verformbare Organ (6) entgegen und gegenüber dem zweiten Organ (7) befindet.
  4. Anordnung nach einem der vorangehenden Ansprüche, dadurch gekennzeichnet, dass die Membran (3) mit einem Sporn (32) ausgestattet ist, der imstande ist, mit dem nicht verformbaren Organ (6) zusammenzuwirken.
  5. Anordnung nach Anspruch 4, dadurch gekennzeichnet, dass der Sporn (32) von oben gesehen die allgemeine Form eines "T" hat.
  6. Anordnung nach einem der Ansprüche 2 bis 5, dadurch gekennzeichnet, dass die Membran (3) mit einem Sporn (33) ausgestattet ist, der imstande ist, mit dem zweiten Organ (7) zusammenzuwirken.
  7. Anordnung nach Anspruch 6, dadurch gekennzeichnet, dass der Sporn (33) von oben gesehen die allgemeine Form eines lateinischen oder griechischen Kreuzes hat.
  8. Anordnung nach einem der Ansprüche 4 bis 7, dadurch gekennzeichnet, dass die Membran (3) einen Kreis bildet, wobei sich der Sporn (32; 33) radial an der Peripherie dieses Kreises erstreckt.
  9. Anordnung nach einem der Ansprüche 2 bis 8, dadurch gekennzeichnet, dass die Platte (5) eine Region (53) aufweist, die das zweite Organ (7) stützt und die vom Rest der Platte durch eine mechanische Schwächungszone (530) getrennt ist, so dass infolge einer mechanischen Beanspruchung, die auf diese Region (53) ausgeübt wird, man ihr eine besondere Ausrichtung in Bezug auf den Rest der Platte (5) verleiht, was erlaubt, die Membran (5) vom zweiten Organ (7) freizusetzen.
  10. Anordnung nach vorangehendem Anspruch, dadurch gekennzeichnet, dass die Region (53) in Bezug auf den Rest der Platte (5) ein Anhängsel bildet, wobei sich die Schwächungszone (530) an ihrer Grenze befindet.
EP10721456.1A 2009-05-12 2010-05-12 Anordnung aus einer filtrationsmembran und einer trägerplatte Active EP2429705B1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0953120A FR2945457B1 (fr) 2009-05-12 2009-05-12 Ensemble forme d'une membrane de filtration et d'une plaque de support, ainsi que dispositif de test microbiologique en faisant usage.
PCT/EP2010/056567 WO2010130792A1 (fr) 2009-05-12 2010-05-12 Ensemble forme d'une membrane de filtration et d'une plaque de support

Publications (2)

Publication Number Publication Date
EP2429705A1 EP2429705A1 (de) 2012-03-21
EP2429705B1 true EP2429705B1 (de) 2016-12-07

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Application Number Title Priority Date Filing Date
EP10721456.1A Active EP2429705B1 (de) 2009-05-12 2010-05-12 Anordnung aus einer filtrationsmembran und einer trägerplatte

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US (1) US20120061308A1 (de)
EP (1) EP2429705B1 (de)
JP (1) JP5735489B2 (de)
ES (1) ES2617757T3 (de)
FR (1) FR2945457B1 (de)
WO (1) WO2010130792A1 (de)

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FR2961525B1 (fr) 2010-06-22 2012-12-28 Aes Chemunex Boite de petri comportant des moyens de verrouillage en vue de la constitution d'une pile
FR2961524B1 (fr) 2010-06-22 2012-08-17 Aes Chemunex Boite de petri pourvue de moyens formant temoins d'utilisation
JP6482459B2 (ja) 2012-05-02 2019-03-13 チャールズ リバー ラボラトリーズ， インコーポレイテッド 細胞サンプル中の生存細胞を検出する方法
WO2013166338A2 (en) * 2012-05-02 2013-11-07 Charles River Laboratories, Inc. Cell capture system and use thereof
USD829340S1 (en) * 2017-05-15 2018-09-25 Charles River Laboratories, Inc. Depressor
USD821605S1 (en) * 2017-05-15 2018-06-26 Charles River Laboratories, Inc. Scanning device adapter
JP2019012999A (ja) 2017-06-30 2019-01-24 日本電産株式会社 導波路装置モジュール、マイクロ波モジュール、レーダ装置およびレーダシステム
MX2020002159A (es) 2017-09-06 2020-07-20 Merck Patent Gmbh Conjunto de filtracion y metodo para pruebas microbiologicas.
US20230257692A1 (en) * 2020-07-08 2023-08-17 Rapid Micro Biosystems, Inc. Filtration assemblies, cassettes, systems, and methods for filtration and cell growth

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Publication number Priority date Publication date Assignee Title
FR2801660A1 (fr) * 1999-11-29 2001-06-01 Chemunex Dispositif de manipulation d'un porte-support solide pour contaminants

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US4783321A (en) * 1984-12-18 1988-11-08 Instrumed, Inc. Sterlization container system
JPH0452703Y2 (de) * 1985-12-26 1992-12-10
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JP4158094B2 (ja) * 2003-01-15 2008-10-01 株式会社クニムネ 尿検体の採取・保存器具
DE20302819U1 (de) * 2003-02-21 2003-05-08 Filtertek Sa Filter für medizinische und Laborzwecke, insbesondere für Blutanalysen u.dgl.
DE10339083B4 (de) * 2003-08-26 2014-12-24 Mahle Filtersysteme Gmbh Gasfilter mit einer Mehrzahl an Befestigungseinrichtungen zur lösbaren Halterung eines Ringfilterelementes
JP4779433B2 (ja) * 2004-08-26 2011-09-28 パナソニック株式会社 ろ過フィルタデバイス
TWM303375U (en) * 2006-05-19 2006-12-21 Inst Of Occupational Safety & Health Council Of Labor Affairs Dust sampler
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Also Published As

Publication number Publication date
WO2010130792A1 (fr) 2010-11-18
JP5735489B2 (ja) 2015-06-17
US20120061308A1 (en) 2012-03-15
EP2429705A1 (de) 2012-03-21
FR2945457B1 (fr) 2011-07-15
ES2617757T3 (es) 2017-06-19
JP2012526534A (ja) 2012-11-01
FR2945457A1 (fr) 2010-11-19

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