EP2353717A1 - Plaque de microtitration - Google Patents

Plaque de microtitration Download PDF

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Publication number
EP2353717A1
EP2353717A1 EP11000810A EP11000810A EP2353717A1 EP 2353717 A1 EP2353717 A1 EP 2353717A1 EP 11000810 A EP11000810 A EP 11000810A EP 11000810 A EP11000810 A EP 11000810A EP 2353717 A1 EP2353717 A1 EP 2353717A1
Authority
EP
European Patent Office
Prior art keywords
plate
microtiter plate
microtiter
vessels
plastic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP11000810A
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German (de)
English (en)
Other versions
EP2353717B1 (fr
Inventor
Thomas Uschkureit
Christian Ziegmann
Hanna Rethwisch
Carsten Koch
Matthias Haak
Martin Seippel
Rainer Jürgen Schulz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
Original Assignee
Eppendorf SE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eppendorf SE filed Critical Eppendorf SE
Publication of EP2353717A1 publication Critical patent/EP2353717A1/fr
Application granted granted Critical
Publication of EP2353717B1 publication Critical patent/EP2353717B1/fr
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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50855—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates using modular assemblies of strips or of individual wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/023—Adapting objects or devices to another adapted for different sizes of tubes, tips or container
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/028—Modular arrangements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates

Definitions

  • the invention relates to a microtiter plate having a plurality of wells in a plate having at least one line of weakness, which divides the plate into different segments.
  • Microtiter plates are used for a variety of microbiological, molecular biological, cell biological and immunological operations.
  • microtiter plates are used for the PCR or the cultivation of microorganisms or cells.
  • Microplates are only partially exploited in many laboratories. This is based in particular on the fact that the reagents used are expensive and the smallest possible number of reactions is carried out. Common applications, including qualitative PCR, often only require up to a maximum of 24 reactions. As a result, in a 96-well microtiter plate, only a portion of the vessels are used and the microtiter plate is disposed of with a portion of unused vessels.
  • a microtiter plate which has a frame-like base and a plurality of vascular strips.
  • the vascular strips are removable from the base and individual vessels are separable from each strip.
  • the vessels are connected to each other by cross-sectionally T-shaped connecting elements which hold the vessels in a plane, rectilinear row, when the vascular strips are either held in or removed from the base.
  • the T-shaped fasteners are easily destructible to allow easy separation of individual vessels. This construction is expensive. In addition, it is also expansive when it is equipped with only a few vascular strips, since the base is not adaptable to the number of vessels required.
  • Corning commercializes 96-well microtiter plates under the trade designation "Thermowell® 96 Well Polypropylene PCR Microplate".
  • the wells are arranged in vessels that protrude beyond the bottom of the plate and slightly beyond the top of the plate.
  • the skirt equally protrudes beyond the bottom and top of the plate.
  • Plate, jars and apron are made in one piece from polypropylene.
  • a microtiter plate for PCR which comprises a plurality of vessels held together by a plate in matrix arrangement.
  • the plate is divided into segments of predetermined size by one or more slots extending substantially across the width of the plate.
  • the slots extend through the entire wall of the panel, with adjacent segments interconnected by one or more connection areas associated with each of the slots.
  • the connecting portions are formed by substantially circular portions extending across the slots, the circular portions being weakened at their periphery to facilitate detachment from the disc.
  • the order Slots and connecting areas are suitable for facilitating the division of the microtiter plate into segments of a predetermined size, which then fit into a thermal cycler without interfering with each other.
  • the plate can be broken by bending over the slots. Alternatively, it may be cut using a pair of scissors, a knife, a scalpel or other cutting tool. Remains of material remaining at the joint areas should be removed with a cutting tool.
  • microtiter plates for the PCR are made of polypropylene, because polypropylene is neutral to DNA and has a sufficient temperature resistance.
  • the known PCR plate can be dismantled only after multiple bending back and forth adjacent segments to the line of weakness and tearing apart of the segments.
  • the user therefore preferably uses a pair of scissors or other cutting tools.
  • sharp edges may remain at which the user can injure or cut into protective gloves of the user or can tear them, so that there is a risk of contamination for sample and / or user.
  • the object of the invention is to provide a microtiter plate which facilitates targeted disassembly into different segments and does not break unintentionally during use or during transport.
  • microtiter plate with the features of claim 1.
  • the plastic microtiter plate according to the invention has a plurality of depressions in a plate having at least one line of weakness which divides the plate into different segments, the line of weakness comprising a weld line formed by spraying various segments of the plate together.
  • the weakening line comprises a weld line.
  • the weld line is created by injecting the various segments of the plate together during injection molding. If the adjacent segments of the plate are injection-molded simultaneously, separately plasticized plastic compounds are introduced into the cavities of the tool, which are assigned to the different segments. The flow fronts of the plasticized plastic masses meet and there forms the weld line.
  • the injection mold When injection molding a microtiter plate having a reduced cross-section at the line of weakness, the injection mold has a reduced gap in the region of the line of weakness. In the gap, the flow fronts slow down, so that they meet very evenly and in a defined position.
  • the line of weakness can be defined solely by the weld line. In the weld line, the strength of the plate is reduced so that it is well breakable along the line of weakness.
  • the plate can be produced by the coinjection process, whereby different segments are injected simultaneously. Different segments of the plate can also be sprayed one after the other using transfer technology, turning or shifting technology or core retraction technique. In this case, at least one segment is first sprayed and at least one further segment is injection-molded onto the sprue produced in this way in a further injection molding step. During injection molding, the flow front of the plasticized plastic mass hits the edge of the already solidified Plastic compound and melts this. This also creates a weakened weld line, which favors a clean breakage of the plate.
  • the different segments can be injected from the same plastic. Further, they may be molded from the same plastic, wherein the material of the different segments may have the same color or different colors. Furthermore, the different segments can be injected from different plastics. By using different plastics for the different segments of the plate, the fracture behavior can be favorably influenced.
  • microtiter plate with the features of claim 2.
  • the plastic microtiter plate according to the invention has a plurality of depressions in a plate which has at least one line of weakness which divides the plate into different segments, wherein the plate is made of an amorphous plastic or of a fiber-reinforced, semi-crystalline plastic.
  • the polypropylene used for conventional PCR plates has a relatively tough behavior. It has namely a relatively low stiffness and thus a relatively low modulus of elasticity (Young's modulus) and a relatively high elongation at break, so that it can not be broken in principle. This also applies to other semi-crystalline materials.
  • amorphous materials are used, which are basically glassy and highly transparent. Amorphous materials have a relatively high modulus of elasticity and a relatively low elongation at break. Therefore, amorphous materials are relatively stiff, making them difficult to bend. Also, they tend to a sudden, uncontrolled break. In itself, therefore, they do not seem suitable for the Production of a microtiter plate, which should be separable into defined segments.
  • a plate made of an amorphous plastic along a line of weakness is good breakable, on which the plate has a reduced wall thickness and / or one or more holes and / or a weld line.
  • This can also be achieved with a plate made of fiber-reinforced, semi-crystalline plastic.
  • the fiber-reinforced, partially crystalline plastic has an increased modulus of elasticity and a reduced elongation at break compared to the semi-crystalline plastic. In combination with the weakening line, this also leads to a good fracture behavior.
  • microtiter plate with the features of claim 3.
  • the plastic microtiter plate according to the invention has a multiplicity of depressions in a plate which has at least one line of weakness which divides the plate into different segments, the line of weakness comprising a groove on the underside of the plate.
  • a good breaking behavior is achieved by the line of weakness with the groove on the underside of the plate.
  • the plate breaks due to the notch effect on the groove, without a multiple back and forth bending is required. This results in a clean break.
  • the arrangement of the groove on the underside favors the formation of the wells in vessels, which protrude from the underside of the plate.
  • the vessels adjacent to the line of weakness are swung apart and do not disturb the breaking of the plate.
  • this arrangement avoids that the vessels adjacent to the line of weakness are damaged by colliding forces when the plate is broken.
  • the groove expands at least in sections towards the underside of the plate. This is manufacturing technology advantageous because it favors the demolding of the molding.
  • the groove is V-shaped, U-shaped or trapezoidal. Most preferably, it is V-shaped, because a line is defined by the converging, sloping walls on which the forces are concentrated when breaking and along which the break occurs.
  • the good fracture behavior is characterized by the fact that the plate preferably breaks once along the line of weakness after selectively bending once around the line of weakness to an extent that significantly exceeds the amount of bending due to usual handling and transport stresses, avoiding sharp breaklines or occur only to a small extent.
  • the bending angle, which leads to breakage is preferably at least 5 ° and can be up to 180 °. More preferably, the bending angle leading to breakage is at least 10 °, more preferably at least 15 °.
  • the microtiter plate does not break unintentionally during handling and transport and can be easily dismantled into segments.
  • a key application advantage is that the broken-apart segments are not sharp-edged.
  • the weakening line comprises at least one groove in the underside and / or in the top of the plate and / or at least one hole in the plate.
  • the weakening line may have a single groove or a plurality of grooves arranged one behind the other.
  • the hole may be slit-shaped or circular.
  • the line of weakness may have a single hole, preferably slit-shaped, or a series of holes which may be slit-shaped or circular.
  • the groove or the hole have a notch effect, which promotes good fracture behavior.
  • the groove is V-shaped.
  • the plate next to the groove has a wall thickness of 1 to 3 mm and / or in the groove has a wall thickness of 0.1 to 1 mm. Further preferably, the plate next to the groove has a wall thickness of 1.5 to 2.5 mm and / or in the groove has a wall thickness of 0.2 to 0.4 mm. Further preferably, the plate in the groove has a wall thickness of 0.25 to 0.35 mm, preferably of about 0.3 mm.
  • the weakening line extends parallel to the narrow sides and / or the longitudinal sides of the plate.
  • the line of weakness extends only parallel to the narrow sides or exclusively parallel to the longitudinal sides of the plate.
  • the weakening lines terminate in edges of the plate and / or in recesses in the edges of the plate. This facilitates the breaking of the plate into different segments.
  • the plate is made of a plastic having an E-modulus of at least 1500 N / mm 2 . According to a further embodiment, the plate is made of a plastic having an E-modulus of at least 2000 N / mm 2 .
  • the plate is made of polycarbonate (PC), cyclo-olefin copolymer (COC), cyclo-olefin polymer (COP), polystyrene (PS) or polymethylmethacrylate (PMMA) or fiber-reinforced polypropylene (PP) or polyethylene (PE ) or other polyolefin.
  • PC polycarbonate
  • COC cyclo-olefin copolymer
  • COP cyclo-olefin polymer
  • PS polystyrene
  • PMMA polymethylmethacrylate
  • PP polymethylmethacrylate
  • PE polyethylene
  • the plate is made of a polycarbonate having an elongation at break of at most 100 to 120%.
  • the plate is made of a reinforced by long glass fibers semi-crystalline plastic.
  • a semi-crystalline plastic reinforced by long glass fibers the fibers lay parallel to the weld line when spraying different segments together so that no reinforcement occurs in the weld line.
  • the material in the weld line fails at the interface between the fiber reinforced area and the adjacent fiber free zone.
  • a reinforcement of a partially crystalline plastic with short glass fibers leads to an increased rigidity of the plate.
  • the short glass fibers also reinforced the board beyond the weld line, such that the board is only breakable under more severe deformation than when reinforcing the semi-crystalline material with long glass fibers.
  • the depressions can have different shapes. According to one embodiment, they are pot-shaped (cylindrical) and / or cup-shaped (for example spherical shell-shaped) and / or conical (conical). You can also have different shapes in different sections, for example, in an upper area cylindrical and conical below and cup-shaped at the bottom.
  • the microtiter plate in different positions wells of different shape and / or in different segments wells of different shapes or exclusively wells with the same shape.
  • the variation of the shape of the wells can thus refer to wells on different positions of the same microtiter plate, on wells in different segments of the microtiter plate or on different microtiter plates. Preference is given to a microtiter plate in which the entirety of the wells have only a single matching shape.
  • the invention includes possible embodiments in which the surface of the microtiter plate varies. For example, over the plate protruding edges of the wells may be different. This different expression can refer to individual positions of the same microtiter plate, to different segments of the same microtiter plate or to different microtiter plates. However, a microtiter plate is preferred in which the surface is uniform over all segments.
  • the microtiter plate has 96 wells or a multiple of 96 wells (eg 384 or 1536 wells). According to another embodiment, the microtiter plate fulfills one or more of the above-mentioned standards ANSI / SBS.
  • the microtiter plate has one, two or three lines of weakness, so that it can be broken into two, three or four segments. The resulting segments are easy to handle.
  • the microtiter plate can also be provided with more lines of weakness. In particular, it can be provided with lines of weakness between all adjacent columns of depressions or between all adjacent rows of depressions, so that strips comprising one column or one row of juxtaposed depressions can be separated off. But it can also be present between all adjacent columns and all adjacent rows lines of weakness, so that segments with an arbitrary number of wells can be separated. In particular, segments are separable comprising only a single recess.
  • the depressions may be formed in a solid plate.
  • the recesses are arranged in vessels, which are connected to the plate. This favors wells with a relatively large volume. Furthermore, it can be ensured by a suitable choice of the materials of the plate and the vessels that on the one hand, the plate has the required stability and is breakable and on the other hand comes in the wells filled sample liquid with a suitable plastic in contact. In particular, this makes it possible to carry out microtiter plates for the PCR in such a way that the plate is relatively stiff and the vessels consist of a plastic suitable for the PCR. In principle, it is also possible that the plate and the vessels are made of the same material, wherein the vessels and the plate may have the same or different colors.
  • the vessels may be designed so that they do not protrude beyond the underside or the top of the plate.
  • the vessels protrude from the underside and / or the top of the plate. This favors vessels with a relatively large filling volume.
  • microtiter plates in which the vessels project from the underside of the plate are particularly well suited for use in PCR thermal cyclers, since the heat exchange can take place directly between the plate of the thermocycler and the walls of the vessels. If the vessels project over the top of the plate, this is advantageous for sealingly attaching a cover sheet directly to the upper edges of the vessels.
  • the plate and the vessels are integrally connected to each other.
  • the plate and the vessels can be integrally connected to each other in particular by injection molding.
  • the plate has a plurality of holes and the vessels are integrally connected by injection molding on the edges of the holes with the plate.
  • the plate consists of a first plastic and the vessels consist of a different from the first plastic second plastic. According to another embodiment, plate and vessels consist of the same plastic.
  • a microtiter plate in which the vessels are integrally connected to the plate by injection molding on the edges of the holes and the vessels and the plate have different plastics is in EP 1 161 994 B2 described.
  • the plate is made of a relatively rigid plastic.
  • plastic for the plate is eg PC and as plastic for the vessels for use in the PCR is eg PP and for use in the oxygen supply
  • silicone can be used for samples.
  • This microtiter plate can be made very dimensionally stable and with very thin-walled vessels for good heat transfer or oxygen supply of samples.
  • a microtiter plate in which the plate has a plurality of holes and vessels of the same plastic as the plate are integrally connected by injection to the edges of the holes in the plate is shown in EP 1 346 772 A2 described.
  • This microtiter plate has the advantage of high dimensional stability and stability.
  • the plate and the vessels may be made of PP, for example. Plastics of the plate and the vessels may have the same color or different colors.
  • the plate according to the invention can according to the plate according to EP 1 161 994 B2 / US 2001051112 A1 or EP 1 346 772 A2 / US 2003 180 192 A1 executed or manufactured.
  • vessels are connected to the plate by clipping, screwing, gluing, welding, sealing or otherwise solvable or non-detachable.
  • the plate has segments which consist of different plastics and / or have different colors.
  • the invention relates to a method for manufacturing a microtiter plate made of plastic with a plurality of wells in a plate having at least one line of weakness, which divides the plate into different segments, wherein the different segments of the plate are sprayed together along the line of weakness.
  • the method is a coinjection method, a conversion method, a rotation or displacement method or a core withdrawal method.
  • top and bottom refer to an orientation of the microtiter plate, wherein the wells are arranged with the openings above and with its closed end at the bottom.
  • matching elements have the same reference numerals.
  • the description of the matching elements is valid for all embodiments having them.
  • a microtiter plate 1.1 comprises a plate 2.1 and a plurality of vessels 3.1. In the eight columns and in twelve rows, a total of 96 vessels 3.1 are arranged like a matrix. In the vessels 3.1 depressions 4.1 are arranged.
  • the plate 2.1 is substantially rectangular with two parallel longitudinal sides 5.1, 5.2 and two parallel narrow sides 6.1, 6.2.
  • the corners between adjacent longitudinal sides 5 and narrow sides 6 may have chamfers 7.1 to 7.4.
  • centering holes 8.1 to 8.4 are arranged.
  • the plate has 2.1 printed on the top 9, lasered or raised numbers 1 to 12, which mark the columns of the vessels 3.1.
  • the plate has 2.1 printed on the top 9, lasered or raised letters A to H, which identify the rows of vessels 3.1.
  • the plate 2.1 has in the bottom 11 between the third and fourth column, the sixth and seventh column and the ninth and tenth column each have a V-shaped groove 12.1, 12.2, 12.3.
  • the V-shaped grooves 12.1, 12.2, 12.3 have a to the bottom 11 of the plate 2.1 expanding towards cross section.
  • the wall thickness of the plate 2.1 next to the grooves 12.1, 12.2, 12.3 is 1 mm to 3 mm, preferably about 2 mm.
  • the residual wall thickness of the plate is 0.2 mm to 0.4 mm, preferably about 0.3 mm.
  • the grooves 12.1, 12.2, 12.3 terminate in slot-shaped recesses 13.11, 13.12, 13.21, 13.22, 13.31, 13.32, extending in the direction of the grooves 12.1, 12.2, 12.3 from the longitudinal sides 5.1, 5.2 of the plate 2.1 to the outer rows A. and H of the vessels 3.1 extend.
  • the vessels 3.1 are molded onto the edges of the holes 4. They have an over the top 9 of the plate 2.1 slightly projecting, annular upper edge 14 and a projecting beyond the bottom 11 of the plate 2.1, conical portion 15 and a cup-shaped portion 16 at the bottom. At the top 9 of the plate 2.1 and at the bottom 11 of the plate 2.1, the vessels 3.1 each have a projection 17.1, 17.2 or radial projection, which supports the vessels on the top 9 and the bottom 11 of the plate 2.1.
  • the plate 2.1 is injection-molded, for example, made of polycarbonate.
  • the vessels 3.1 are injection-molded, for example, from polypropylene or from a silicone.
  • the microtiter plate 1.1 is preferably produced in a multi-component injection molding process. It can in particular the characteristics of the plate and the vessels according to EP 1 161 994 B2 or US2001051112 A1 and prepared according to the method described in the aforementioned publications. The relevant statements in the cited references are incorporated by reference in the present application. Alternatively, the microtiter plate 1.1, the characteristics of the plate and the vessels according to EP 1 346 772 A2 or US2003180192 A1 and prepared according to the method described therein. The relevant embodiments of the cited references are incorporated by reference in the present application.
  • the plate 1.2 according to Fig. 2 differs from the above-described characterized in that the lines of weakness 20.12, 20.22, 20.32 instead of the V-grooves 12.1, 12.2, 12.3 each have a series of rectangular slots 21.1, 21.2, 21.3. Furthermore, the weakening lines 20.12, 20.22, 20.32 each comprise a weld line 19.12, 19.22, 19:32. The weld line 19.12, 19.22, 19.32 in combination with the plastic used for the plate 2.2 and the notch effect due to the slots 21.1, 22.2, 21.3 also leads to a haptic pleasing fracture behavior and a smooth, supple fracture surface, if individual segments 18.1 accordingly Fig. 1 f) with respect to the remainder 18.2, 18.3, 18.4 of the plate 2.2 are folded up.
  • the microtiter plate 1.3 of Fig. 3 differs from the microtiter plate 1.1 in that in the various segments vessels 3.1, 3.2, 3.3, 3.4 different shape with corresponding recesses 4.1, 4.2, 4.3, 4.4 are arranged, the shape of the recesses 4.1, 4.2, 4.3, 4.4 in the respective segment 18.1, 18.2, 18.3, 18.4 are each uniform.
  • the left segment 18.1 has vessels 3.1 whose shape corresponds to the shape of the vessels 3.1 of the microtiter plate 1.1.
  • the vessels 3.2 have a circular cylindrical shape.
  • the vessels 3.3 have a cup shape.
  • the vessels 3.4 again have a cylindrical shape, these cylinders being less high than in the vessels of the segment 18.2.
  • the height of these vessels 3.4 is only about one third of the height of the vessels 3.2.
  • the vessels 3.1, 3.2, 3.3, 3.4 of different shapes can be used for various investigations, in which vessels with different volumes or with different vessel shapes are useful.
  • microtiter plate 1.3 Due to the lines of weakness 20.11, 20.21, 20.31 and the microtiter plate 1.3 in the same manner as the microtiter plate 1.1 can be divided into segments.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
EP20110000810 2010-02-05 2011-02-02 Plaque de microtitration Active EP2353717B1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE201010008036 DE102010008036A1 (de) 2010-02-05 2010-02-05 Mikrotiterplatte

Publications (2)

Publication Number Publication Date
EP2353717A1 true EP2353717A1 (fr) 2011-08-10
EP2353717B1 EP2353717B1 (fr) 2012-12-26

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Country Status (3)

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EP (1) EP2353717B1 (fr)
CN (1) CN102151590A (fr)
DE (1) DE102010008036A1 (fr)

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EP2539073B1 (fr) * 2010-02-22 2014-12-17 4titude Ltd. Lamelle aux multipuits
WO2019178703A1 (fr) 2018-03-23 2019-09-26 Ambartsoumian Gourgen Porte-tube d'acp
WO2020043687A1 (fr) * 2018-08-27 2020-03-05 Sabic Global Technologies B.V. Agencement à coupelles multiples pour emballage de produits alimentaires

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CN102943031A (zh) * 2012-10-30 2013-02-27 无锡耐思生物科技有限公司 96孔聚合酶链式反应联排管结构
CN104015306B (zh) * 2014-06-09 2016-08-31 东莞市积健生物科技有限公司 Pcr板注塑成型模具及其方法
US10137453B2 (en) * 2014-12-10 2018-11-27 Biotix, Inc. Static-defeating apparatus for pipette tips
EP3393663B1 (fr) * 2015-12-22 2021-04-14 3M Innovative Properties Company Méthode de séparation d'un échantillon de test aqueux
JP6635532B1 (ja) * 2019-03-25 2020-01-29 グローバルサイエンス株式会社 サンプルカップ集合体

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EP2353717B1 (fr) 2012-12-26
DE102010008036A1 (de) 2011-08-11

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